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- Growth and differentiation properties of pikromycin-producing Streptomyces venezuelae ATCC15439
- Ji-Eun Kim , Joon-Sun Choi , Jung-Hye Roe
- J. Microbiol. 2019;57(5):388-395. Published online February 5, 2019
- DOI: https://doi.org/10.1007/s12275-019-8539-3
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- 6 Crossref
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Abstract
- Streptomycetes naturally produce a variety of secondary metabolites, in the process of physiological differentiation. Streptomyces venezuelae differentiates into spores in liquid media, serving as a good model system for differentiation and a host for exogenous gene expression. Here, we report the growth and differentiation properties of S. venezuelae ATCC- 15439 in liquid medium, which produces pikromycin, along with genome-wide gene expression profile. Comparison of growth properties on two media (SPA, MYM) revealed that the stationary phase cell viability rapidly decreased in SPA. Submerged spores showed partial resistance to lysozyme and heat, similar to what has been observed for better-characterized S. venezuelae ATCC10712, a chloramphenicol producer. TEM revealed that the differentiated cells in the submerged culture showed larger cell size, thinner cell wall than the aerial spores. We analyzed transcriptome profiles of cells grown in liquid MYM at various growth phases. During transition and/or stationary phases, many differentiationrelated genes were well expressed as judged by RNA level, except some genes forming hydrophobic coats in aerial mycelium. Since submerged spores showed thin cell wall and partial resistance to stresses, we examined cellular expression of MreB protein, an actin-like protein known to be required for spore wall synthesis in Streptomycetes. In contrast to aerial spores where MreB was localized in septa and spore cell wall, submerged spores showed no detectable signal. Therefore, even though the mreB transcripts are abundant in liquid medium, its protein level and/or its interaction with spore wall synthetic complex appear impaired, causing thinner- walled and less sturdy spores in liquid culture.
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Citations
Citations to this article as recorded by
- Biosynthesis of Arcyriaflavin F from Streptomyces venezuelae ATCC 10712
Hung‐En Lai, Agata Kennedy, Lewis Tanner, Emma A. Bartram, Soo Mei Chee, Paul S. Freemont, Simon J. Moore
ChemBioChem.2024;[Epub] CrossRef - Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439
Shuai Li, Zhong Li, Guoqiang Zhang, Vlada B. Urlacher, Li Ma, Shengying Li
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Journal of Applied Microbiology.2024;[Epub] CrossRef - Comparative Metagenomics Reveals Microbial Signatures of Sugarcane Phyllosphere in Organic Management
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Frontiers in Microbiology.2021;[Epub] CrossRef - Lysine acetylation of the housekeeping sigma factor enhances the activity of the RNA polymerase holoenzyme
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Microorganisms.2020; 8(12): 2013. CrossRef
- Biosynthesis of Arcyriaflavin F from Streptomyces venezuelae ATCC 10712
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