MSK
Search
- Page Path
- HOME > Search
Research Support, Non-U.S. Gov't
- Biochemical Properties and Physiological Roles of NADP-Dependent Malic Enzyme in Escherichia coli
- Baojuan Wang , Peng Wang , Enxia Zheng , Xiangxian Chen , Hanjun Zhao , Ping Song , Ruirui Su , Xiaoning Li , Guoping Zhu
- J. Microbiol. 2011;49(5):797-802. Published online November 9, 2011
- DOI: https://doi.org/10.1007/s12275-011-0487-5
- 43 View
- 0 Download
- 21 Crossref
-
Abstract
- Malic enzymes catalyze the reversible oxidative decarboxylation of L-malate using NAD(P)+ as a cofactor. NADP-dependent malic enzyme (MaeB) from Escherichia coli MG1655 was expressed and purified as a fusion protein. The molecular weight of MaeB was about 83 kDa, as determined by SDS-PAGE. The recombinant MaeB showed a maximum activity at pH 7.8 and 46°C. MaeB activity was dependent on the presence of Mn2+ but was strongly inhibited by Zn2+. In order to understand the physiological roles, recombinant E. coli strains (icdNADP/ΔmaeB and icdNAD/ΔmaeB) containing NADP-dependent isocitrate dehydrogenase (IDH), or engineered NAD-dependent IDH with the deletion of the maeB gene, were constructed using homologous recombination. During growth on acetate, icdNAD/ΔmaeB grew poorly, having a growth rate only 60% that of the wild-type strain (icdNADP). Furthermore, icdNADP/ΔmaeB exhibited a 2-fold greater adaptability to acetate than icdNAD/ΔmaeB, which may be explained by more NADPH production for biosynthesis in icdNADP/ΔmaeB due to its NADP-dependent IDH. These results indicated that MaeB was important for NADPH production for bacterial growth on acetate. We also observed that MaeB activity was significantly enhanced (7.83-fold) in icdNAD, which was about 3-fold higher than that in icdNADP, when switching from glucose to acetate. The marked increase of MaeB activity was probably induced by the shortage of NADPH in icdNAD. Evidently, MaeB contributed to the NADPH generation needed for bacterial growth on two carbon compounds.
-
Citations
Citations to this article as recorded by
- TA-Cloning for Diabetes Treatment: Expressing Corynebacterium Malic Enzyme Gene in E. coli
Somayeh Farahmand, Saber SamadiAfshar, Ladan Hosseini
Current Microbiology.2024;[Epub] CrossRef - Recent advances, challenges and metabolic engineering strategies in the biosynthesis of 3‐hydroxypropionic acid
Bo Liang, Guannan Sun, Xinping Zhang, Qingjuan Nie, Yukun Zhao, Jianming Yang
Biotechnology and Bioengineering.2022; 119(10): 2639. CrossRef - Metabolic engineering of Halomonas elongata: Ectoine secretion is increased by demand and supply driven approaches
Karina Hobmeier, Martin Oppermann, Natalie Stasinski, Andreas Kremling, Katharina Pflüger-Grau, Hans Jörg Kunte, Alberto Marin-Sanguino
Frontiers in Microbiology.2022;[Epub] CrossRef - Improved production of 2′-fucosyllactose in engineered Saccharomyces cerevisiae expressing a putative α-1, 2-fucosyltransferase from Bacillus cereus
Mingyuan Xu, Xiangfeng Meng, Weixin Zhang, Yu Shen, Weifeng Liu
Microbial Cell Factories.2021;[Epub] CrossRef - One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli
Ningyu Lai, Yuanchan Luo, Peng Fei, Peng Hu, Hui Wu
Synthetic and Systems Biotechnology.2021; 6(3): 144. CrossRef -
Transcriptomics and Methylomics Study on the Effect of Iodine-Containing Drug FS-1 on
Escherichia Coli
ATCC BAA-196
Ilya S Korotetskiy, Ardak B Jumagaziyeva, Sergey V Shilov, Tatyana V Kuznetsova, Auyes N Myrzabayeva, Zhanar A Iskakbayeva, Aleksandr I Ilin, Monique Joubert, Setshaba Taukobong, Oleg N Reva
Future Microbiology.2021; 16(14): 1063. CrossRef - Enzymatic characterization of blood lymphocytes in various clinical and pathogenetic variants of respiratory allergy
A. M. Lazareva, O. A. Kolenchukova, S. V. Smirnova
Medical Immunology (Russia).2021; 23(1): 107. CrossRef - The Protein-Protein Interaction Network Reveals a Novel Role of the Signal Transduction Protein PII in the Control of c-di-GMP Homeostasis in Azospirillum brasilense
Edileusa C. M. Gerhardt, Erick Parize, Fernanda Gravina, Flávia L. D. Pontes, Adrian R. S. Santos, Gillize A. T. Araújo, Ana C. Goedert, Alysson H. Urbanski, Maria B. R. Steffens, Leda S. Chubatsu, Fabio O. Pedrosa, Emanuel M. Souza, Karl Forchhammer, Ele
mSystems.2020;[Epub] CrossRef - Metabolic changes in peripheral blood lymphocytes from children with recurrent respiratory infections
L. M. Kurtasova, N. A. Shakina, T. V. Lubnina
Russian Journal of Infection and Immunity.2020; 10(3): 515. CrossRef - The NADP-dependent malic enzyme MaeB is a central metabolic hub controlled by the acetyl-CoA to CoASH ratio
Luciano F. Huergo, Gillize A.T. Araújo, Adrian S.R. Santos, Edileusa C.M. Gerhardt, Fabio O. Pedrosa, Emanuel M. Souza, Karl Forchhammer
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics.2020; 1868(9): 140462. CrossRef - Rediscovering Acetate Metabolism: Its Potential Sources and Utilization for Biobased Transformation into Value-Added Chemicals
Hyun Gyu Lim, Ji Hoon Lee, Myung Hyun Noh, Gyoo Yeol Jung
Journal of Agricultural and Food Chemistry.2018; 66(16): 3998. CrossRef - Enhanced performance of the methylerythritol phosphate pathway by manipulation of redox reactions relevant to IspC, IspG, and IspH
Jia Zhou, Liyang Yang, Chonglong Wang, Eui-Sung Choi, Seon-Won Kim
Journal of Biotechnology.2017; 248: 1. CrossRef - Rational design of a synthetic Entner–Doudoroff pathway for improved and controllable NADPH regeneration
Chiam Yu Ng, Iman Farasat, Costas D. Maranas, Howard M. Salis
Metabolic Engineering.2015; 29: 86. CrossRef - Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation
Eleni Theodosiou, Oliver Frick, Bruno Bühler, Andreas Schmid
Microbial Cell Factories.2015;[Epub] CrossRef - NADPH-generating systems in bacteria and archaea
Sebastiaan K. Spaans, Ruud A. Weusthuis, John van der Oost, Servé W. M. Kengen
Frontiers in Microbiology.2015;[Epub] CrossRef - Central metabolic responses to the overproduction of fatty acids in Escherichia coli based on 13C‐metabolic flux analysis
Lian He, Yi Xiao, Nikodimos Gebreselassie, Fuzhong Zhang, Maciek R. Antoniewicz, Yinjie J. Tang, Lifeng Peng
Biotechnology and Bioengineering.2014; 111(3): 575. CrossRef - Optimal cofactor swapping can increase the theoretical yield for chemical production in Escherichia coli and Saccharomyces cerevisiae
Zachary A. King, Adam M. Feist
Metabolic Engineering.2014; 24: 117. CrossRef - Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation
Won-Heong Lee, Myoung-Dong Kim, Yong-Su Jin, Jin-Ho Seo
Applied Microbiology and Biotechnology.2013; 97(7): 2761. CrossRef - Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli
Irisappan Ganesh, Sambandam Ravikumar, Si Jae Park, Seung Hwan Lee, Soon Ho Hong
Korean Journal of Chemical Engineering.2013; 30(7): 1443. CrossRef - Optimizing Cofactor Specificity of Oxidoreductase Enzymes for the Generation of Microbial Production Strains—OptSwap
Zachary A. King, Adam M. Feist
Industrial Biotechnology.2013; 9(4): 236. CrossRef - Investigating the effects of perturbations to pgi and eno gene expression on central carbon metabolism in Escherichia coli using 13 C metabolic flux analysis
Yuki Usui, Takashi Hirasawa, Chikara Furusawa, Tomokazu Shirai, Natsuko Yamamoto, Hirotada Mori, Hiroshi Shimizu
Microbial Cell Factories.2012;[Epub] CrossRef
- TA-Cloning for Diabetes Treatment: Expressing Corynebacterium Malic Enzyme Gene in E. coli
First
Prev
TOP
