Journal of Microbiology

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Synthesis of pinene in the industrial strain Candida glycerinogenes by modification of its mevalonate pathway: Tengfei Ma , Hong Zong , Xinyao Lu , Bin Zhuge; J. Microbiol. 2022;60(12):1191-1200. Published online October 24, 2022; DOI: https://doi.org/10.1007/s12275-022-2344-0

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Terpenes have many applications and are widely found in nature, but recent progress in synthetic biology has enabled the use of microorganisms as chassis cells for the synthesis of these compounds. Candida glycerinogenes (C. glycerinogenes) is an industrial strain that may be developed as a chassis for the synthesis of terpenes since it has a tolerance to hyperosmolality and high sugar, and has a complete mevalonate (MVA) pathway. However, monoterpenes such as pinene are highly toxic, and the tolerance of C. glycerinogenes to pinene was investigated. We also measured the content of mevalonate and squalene to evaluate the strength of the MVA pathway. To determine terpene synthesis capacity, a pathway for the synthesis of pinene was constructed in C. glycerinogenes. Pinene production was improved by overexpression, gene knockdown and antisense RNA inhibition. Pinene production was mainly enhanced by strengthening the upstream MVA pathway and inhibiting the production of by-products from the downstream pathway. With these strategies, yield could be increased by almost 16 times, to 6.0 mg/L. Overall, we successfully constructed a pinene synthesis pathway in C. glycerinogenes and enhanced pinene production through metabolic modification.

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Recent advances in genome mining and synthetic biology for discovery and biosynthesis of natural products
Mingpeng Wang, Lei Chen, Zhaojie Zhang, Qinhong Wang
Critical Reviews in Biotechnology.2025; 45(1): 236. CrossRef
Engineering a complete mevalonate pathway in Chlamydomonas reinhardtii for enhanced isoprenoid production
Jingkai Wang, Muhammad Anwar, Jiancheng Li, Lin Dan, Bin Jia, Zhangli Hu
Algal Research.2025; 88: 103987. CrossRef
Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes
Tuo Li, Ximeng Liu, Haoyu Xiang, Hehua Zhu, Xuan Lu, Baomin Feng
Molecules.2024; 29(5): 1127. CrossRef
Acetic acid stress and utilization synergistically enhance squalene biosynthesis in Candida glycerinogenes
Zhenzhen You, Xueqing Du, Hong Zong, Xinyao Lu, Bin Zhuge
Biochemical Engineering Journal.2024; 210: 109413. CrossRef
Recent developments in enzymatic and microbial biosynthesis of flavor and fragrance molecules
Roman M. Dickey, Madan R. Gopal, Priyanka Nain, Aditya M. Kunjapur
Journal of Biotechnology.2024; 389: 43. CrossRef
Recent Advances and Multiple Strategies of Monoterpenoid Overproduction in Saccharomyces cerevisiae and Yarrowia lipolytica
Dong-Xun Li, Qi Guo, Yu-Xin Yang, Shun-Jie Jiang, Xiao-Jun Ji, Chao Ye, Yue-Tong Wang, Tian-Qiong Shi
ACS Synthetic Biology.2024; 13(6): 1647. CrossRef
Gene Editing of Candida glycerinogenes by Designed Toxin–Antitoxin Cassette
Wen Lv, Xinyao Lu, Bin Zhuge, Hong Zong
ACS Synthetic Biology.2024; 13(3): 816. CrossRef
Candida glycerinogenes-Promoted α-Pinene and Squalene Co-production Strategy Based on α-Pinene Stress
Tengfei Ma, Hong Zong, Xinyao Lu, Bin Zhuge
Journal of Agricultural and Food Chemistry.2023; 71(13): 5250. CrossRef

Screening and identification of Aspergillus activity against Xanthomonas oryzae pv. oryzae and analysis of antimicrobial components: Bei Jiang , Zhiying Wang , Chuxuan Xu , Weijia Liu , Donghua Jiang; J. Microbiol. 2019;57(7):597-605. Published online June 27, 2019; DOI: https://doi.org/10.1007/s12275-019-8330-5

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Abstract

To screen for Aspergillus activity against Xanthomonas oryzae pv. oryzae and analyse the antimicrobial components involved, 60 Aspergillus spp. were isolated and purified from fruits, soil and other habitats. As-75, an Aspergillus strain that can antagonize Xanthomonas oryzae pv. oryzae, was identified based on the zone of inhibition formed during co-culture. According to morphological, ITS rDNA gene sequencing and phylogenetic tree results, the strain showed close homology to Aspergillus sclerotiorum. The biochemical characterization tests showed that the fermentation broth of strain As-75 exhibited a high capacity for environmental adaptation. The results of the antimicrobial spectrum experiments demonstrated that As-75 exhibited fairly strong antagonistic activity against five plant pathogenic fungi and six plant pathogenic bacteria in vitro. The fermentation broth of strain As-75 displayed maximum stability under fluorescent illumination at temperatures below 60°C at pH 6.5. A substance with antagonistic activity was obtained from strain As-75 via fractional extraction, silica gel column chromatography and thinlayer chromatography. Through mass spectrometry, nuclear magnetic resonance and electrospray ionization mass spectrometry (ESI-MS) analyses, the target compound was identified as (2Z)-2-butenedioic acid-2-(1-methylethenyl)-4-methyl ester; its molecular weight of 170.06 daltons and formula of C8H10O4 identify it as a novel compound. Trials of the preventative and curative effects demonstrated that compound S1 exhibited a better control efficiency than the control against rice bacterial blight. Additionally, the M1 processing
method
was better, and the efficiency of compound S1 in preventing rice bacterial blight in six rice varieties, TN1, IR24, ZF802, Zhonghua 11, Wuyunjing 21, and Nipponbare, was 78.3%, 77.5%, 74.2%, 75.3%, 70.9%, and 72.1%, respectively.

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Screening and identification of Aspergillus sclerotiorum with activity against Metschnikowia bicuspidata and initial application on "milky disease" in Eriocheir sinensis
Senting Pu, Zhouling Chen, Dong Sheng, Yunmeng Shan, Peilin Zhou, Xinran Shi, Kexin Hao, Shigen Ye
Aquaculture.2025; 595: 741653. CrossRef
Application and antagonistic mechanisms of atoxigenic Aspergillus strains for the management of fungal plant diseases
Suyan Wang, Yanxia Wang, Xinchi Shi, Daniela D. Herrera-Balandrano, Xin Chen, Fengquan Liu, Pedro Laborda, Irina S. Druzhinina
Applied and Environmental Microbiology.2024;[Epub] CrossRef
Screening of indigenous entomopathogenic fungal isolates on plant parasitic nematodes in China
Ming Fang, Jie Sun, Ailing Wang, Hongbo Tang, Lei Wang, Xianqin Wei, Weibin Ruan
European Journal of Plant Pathology.2024; 169(4): 787. CrossRef
Synergy in Rice Immunity: Exploring Strategies of Coordinated Disease Defense Through Receptor-Like Kinases and Receptor- Like Cytoplasmic Kinases
Mengtian Pei, Yingying Cao, Xuze Xie, Ying Cao, Jia Chen, Xi Zhang, Zonghua Wang, Guodong Lu, Shenghang Zhang
Rice Science.2024; 31(6): 643. CrossRef
Antimicrobial cyclic lipopeptides from Bacillus mojovensis B1302 against wheat root rot
Yanjie Yi, Shijie Liu, Shihao Ren, Yunpeng Shen, Xinyue Lin, Jia Shi, Kang Wang, Changfu Zhang
Rhizosphere.2024; 32: 100963. CrossRef
Screening<italic> Streptomyces </italic>against <italic>Xanthomonas axonopodis</italic> pv<italic>. glycines</italic> and study of growth-promoting and biocontrol effect
Xuan-Dong WANG, Sun-Yu-Yue YANG, Run-Jie GAO, Jun-Jie YU, Dan-Pei ZHENG, Feng NI, Dong-Hua JIANG
Acta Agronomica Sinica.2022; 48(6): 1546. CrossRef
Effect of microwave radiation combined with cellulase treatment of soybean residue on the culture of Aspergillus oryzae
Huaixiang Tian, Yao Liu, Li Li, Chen Chen, Haiyan Yu, Xinxin Ma, Juan Huang, Xinman Lou, Haibin Yuan
Food Bioscience.2022; 50: 101988. CrossRef
Compound fermentation supernatants of antagonistic bacteria control Rhizoctonia cerealis and promote wheat growth
Yanjie Yi, Yang Liu, Pengyu Luan, Zhipeng Hou, Yanhui Yang, Ruifang Li, Zhenpu Liang, Xiaoxia Zhang, Shulei Liu
Egyptian Journal of Biological Pest Control.2022;[Epub] CrossRef
Screening of antagonistic bacteria against the blue mold of citrus fruit from soil by a new parallel screening method without prior isolation of single strains
Zhenzhen Sun, Tingting Liu, Zhe Liu, Chaozhen Zeng, Zhixiang Liu
Biological Control.2022; 176: 105066. CrossRef
Berkchaetoazaphilone B has antimicrobial activity and affects energy metabolism
Xudong Ouyang, Jelmer Hoeksma, Gisela van der Velden, Wouter A. G. Beenker, Maria H. van Triest, Boudewijn M. T. Burgering, Jeroen den Hertog
Scientific Reports.2021;[Epub] CrossRef

NMR-based metabolomics reveals the metabolite profiles of Vibrio parahaemolyticus under ferric iron stimulation: Jun Zhou , Chenyang Lu , Dijun Zhang , Chennv Ma , Xiurong Su; J. Microbiol. 2017;55(8):628-634. Published online July 28, 2017; DOI: https://doi.org/10.1007/s12275-017-6551-z

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Abstract

Vibrio parahaemolyticus is a halophilic bacterium endemic to coastal areas, and its pathogenicity has caused widespread seafood poisoning. In our previous research, the protein expression of V. parahaemolyticus in Fe3+ medium was determined using isobaric tags for relative and absolute quantitation (iTRAQ). Here, nuclear magnetic resonance (NMR) was used to detect changes in the V. parahaemolyticus metabolome. NMR spectra were obtained using methanol-water extracts of intracellular metabolites from V. parahaemolyticus under various culture conditions, and 62 metabolites were identified, including serine, arginine, alanine, ornithine, tryptophan, glutamine, malate, NAD+, NADP+, oxypurinol, xanthosine, dCTP, uracil, thymine, hypoxanthine, and betaine. Among these, 21 metabolites were up-regulated after the stimulation of the cells by ferric iron, and 9 metabolites were down-regulated. These metabolites are involved in amino acid and protein synthesis, energy metabolism, DNA and RNA synthesis and osmolality. Based on these results, we conclude that Fe3+ influences the metabolite profiles of V. parahaemolyticus.

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Potential role of alginate in marine bacteria-yeast interactions
Shota Nakata, Ryuichi Takase, Shigeyuki Kawai, Kohei Ogura, Wataru Hashimoto, Jennifer B. Glass
Applied and Environmental Microbiology.2024;[Epub] CrossRef
ZrgA contributes to zinc acquisition in Vibrio parahaemolyticus
Chengkun Zheng, Jun Qiu, Yimeng Zhai, Man Wei, Xiaohui Zhou, Xinan Jiao
Virulence.2023;[Epub] CrossRef
1H NMR-based water-soluble lower molecule characterization and fatty acid composition of Chinese native chickens and commercial broiler
Zhichao Xiao, Wangang Zhang, Hongtao Yang, Ziyu Yan, Changrong Ge, Guozhou Liao, Huawei Su
Food Research International.2021; 140: 110008. CrossRef
Itaconic acid inhibits growth of a pathogenic marine Vibrio strain: A metabolomics approach
Thao Van Nguyen, Andrea C. Alfaro, Tim Young, Saras Green, Erica Zarate, Fabrice Merien
Scientific Reports.2019;[Epub] CrossRef
New Insights Into the Response of Metabolome of Escherichia coli O157:H7 to Ohmic Heating
Xiaojing Tian, Qianqian Yu, Donghao Yao, Lele Shao, Zhihong Liang, Fei Jia, Xingmin Li, Teng Hui, Ruitong Dai
Frontiers in Microbiology.2018;[Epub] CrossRef

Spectral characterization of a pteridine derivative from cyanide-utilizing bacterium Bacillus subtilis - JN989651: S. Durairaju Nisshanthini , Antony K. Teresa Infanta S. , Duraisamy Senthil Raja , Karuppannan Natarajan , M. Palaniswamy , Jayaraman Angayarkanni; J. Microbiol. 2015;53(4):262-271. Published online March 4, 2015; DOI: https://doi.org/10.1007/s12275-015-4138-0

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Abstract

Soil and water samples were collected from various regions of SIPCOT and nearby Vanappadi Lake, Ranipet, Tamilnadu, India. Based on their colony morphology and their stability during subculturing, 72 bacteria were isolated, of which 14 isolates were actinomycetes. Preliminary selection was carried out to exploit the ability of the microorganisms to utilize sodium cyanate as nitrogen source. Those organisms that were able to utilize cyanate were subjected to secondary screening viz., utilization of sodium cyanide as the nitrogen source. The oxygenolytic cleavage of cyanide is dependent on cyanide monooxygenase which obligately requires pterin cofactor for its activity. Based on this, the organisms capable of utilizing sodium cyanide were tested for the presence of pterin. Thin layer chromatography (TLC) of the cell extracts using n-butanol: 5 N glacial acetic acid (4:1) revealed that 10 out of 12 organisms that were able to utilize cyanide had the pterin-related blue fluorescent compound in the cell extract. The cell extracts of these 10 organisms were subjected to high performance thin layer chromatography (HPTLC) for further confirmation using a pterin standard. Based on the incubation period, cell biomass yield, peak height and area, strain VPW3 was selected and was identified as Bacillus subtilis. The Rf value of the cell extract was 0.73 which was consistent with the 0.74 Rf value of the pterin standard when scanned at 254 nm. The compound was extracted and purified by preparative High Performance Liquid Chromatography (HPLC). Characterization of the compound was performed by ultraviolet spectrum, fluorescence spectrum, Electrospray Ionization-Mass Spectrometry (ESI-MS), and Nuclear Magnetic Resonance spectroscopy (NMR). The compound is proposed to be 6-propionyl pterin (2-amino-6- propionyl-3H-pteridin-4-one).

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Cyanide Bioremediation by Bacillus subtilis under Alkaline Conditions
César Julio Cáceda Quiroz, Gabriela de Lourdes Fora Quispe, Milena Carpio Mamani, Gisela July Maraza Choque, Elisban Juani Sacari Sacari
Water.2023; 15(20): 3645. CrossRef
An Overview of Emerging Cyanide Bioremediation Methods
Narges Malmir, Najaf Allahyari Fard, Saeed Aminzadeh, Zahra Moghaddassi-Jahromi, Lukhanyo Mekuto
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Brighter is better: bill fluorescence increases social attraction in a colonial seabird and reveals a potential link with foraging
H. D. Douglas, I. V. Ermakov, W. Gellermann
Behavioral Ecology and Sociobiology.2021;[Epub] CrossRef
Microbial (Enzymatic) Degradation of Cyanide to Produce Pterins as Cofactors
Ramasamy Mahendran, Sabna BS, Murugesan Thandeeswaran, Kiran kG, Muthu Vijayasarathy, Jayaraman Angayarkanni, Gayathri Muthusamy
Current Microbiology.2020; 77(4): 578. CrossRef
Metabolic Processes Preserved as Biosignatures in Iron-Oxidizing Microorganisms: Implications for Biosignature Detection on Mars
Melissa A. Merrill Floyd, Amy J. Williams, Andrej Grubisic, David Emerson
Astrobiology.2019; 19(1): 40. CrossRef
Production and optimization of pterin deaminase from cyanide utilizing bacterium Bacillus cereus AM12
Murugesan Thandeeswaran, Sajitha Bijukumar, Mani Arulkumar, Ramasamy Mahendran, Muthusamy Palaniswamy, Jayaraman Angayarkanni
Biotechnology Research and Innovation.2019; 3(1): 159. CrossRef
Evaluation of Pterin, a Promising Drug Candidate from Cyanide Degrading Bacteria
Ramasamy Mahendran, Murugesan Thandeeswaran, Gopikrishnan Kiran, Mani Arulkumar, K. A. Ayub Nawaz, Jayamanoharan Jabastin, Balraj Janani, Thomas Anto Thomas, Jayaraman Angayarkanni
Current Microbiology.2018; 75(6): 684. CrossRef
Venus' Spectral Signatures and the Potential for Life in the Clouds
Sanjay S. Limaye, Rakesh Mogul, David J. Smith, Arif H. Ansari, Grzegorz P. Słowik, Parag Vaishampayan
Astrobiology.2018; 18(9): 1181. CrossRef
Analeptic agent from microbes upon cyanide degradation
Thandeeswaran Murugesan, Nisshanthini Durairaj, Mahendran Ramasamy, Karunya Jayaraman, Muthusamy Palaniswamy, Angayarkanni Jayaraman
Applied Microbiology and Biotechnology.2018; 102(4): 1557. CrossRef
Pterin function in bacteria
Nathan Feirer, Clay Fuqua
Pteridines.2017; 28(1): 23. CrossRef

Characterization of Exopolysaccharide (EPS) Produced by Weissella hellenica SKkimchi3 Isolated from Kimchi: Min Ju Kim , Ha Na Seo , Tae Sik Hwang , Sung Hun Lee , Doo Hyun Park; J. Microbiol. 2008;46(5):535-541. Published online October 31, 2008; DOI: https://doi.org/10.1007/s12275-008-0134-y

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Abstract: Weissella hellenica SKkimchi3 produces the higher exopolysaccharide (EPS) on sucrose than lactose, glucose, and fructose at pH 5 and 20°C. Sucrose was exclusively used to cultivate SKkimchi3 in all experiments base on the EPS production tests. The molecular mass of EPS, as determined by gel permeation chromatography, was 203,000. 1H and 13C NMR analysis indicated that the identity of EPS may be a glucan. When EPS, starch, and cellulose was treated with α-amylase, glucoamylase, glucosidase, and cellulase, glucose was produced from starch and cellulose but was not produced from EPS. Based on HPLC analysis, elemental analysis, 1H and 13C NMR analysis, and enzymatic hydrolysis tests, EPS was estimated to be a glucan. EPS suspension was not precipitated even by centrifugation at 10,000×g for 60 min, and EPS made the fermented milk and bacterial culture viscous.

Characterization of C-P Lyase gene cluster by in vivo ³¹P-NMR spectroscopy: Lee, Ki Sung , Kwak, In Young; J. Microbiol. 1995;33(4):328-333.

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Abstract: ³¹P-NMR experiment was performed to detect phophonates (Pn) utilization and degradation in the several different C-P lyase mutants of E. coli and in E. aerogenes and the recombinants. The relative peak intensity (RPI) for the standard samples of 0.5 mM methylphosphonate (MPn) and 1.0 mM aminoethylphosphonate in glucose-MOPS medium showed 0.5 : 1.0 ratio. In the case of BW14329 (ΔphnC-P, ΔphoA), RPI did not change significantly after 24 hrs culturing, which means it nearly could not utilize Pn. In vivo ³¹P-NMR spectrum of E. aerogens (BWKL 16627) during 3 hrs starvation showed two intense peaks at 0-2 ppm and at near-10 ppm which indicate intracellular orthophosphate (Pi) and pyrophosphate (PPi), respectively. Both of them might be released by degradation of inorganic polyphosphate pool. When MPn is supplied to the medium as an unique P source, Pi content in the cell has the constant, but PPi seems to be slightly decreased. Recombinants (BWKL 16954) grew slower than E. aerogenes in the glucose-MOPS media with various P sources. In vivo ³¹P-NMR spectrum of recombinant did not show any intense signal in the cell. Surprisingly, under the cultivation adding with MPn, a few intense peaks in the region of Pi AND phospate monoester were detected.

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