Yifan Jiao , Sihui Zhang , Jing Yang , Xin-He Lai , Kui Dong , Yanpeng Cheng , Mingchao Xu , Wentao Zhu , Shan Lu , Dong Jin , Ji Pu , Ying Huang , Liyun Liu , Suping Wang , Jianguo Xu
J. Microbiol. 2022;60(2):147-155. Published online January 7, 2022
Two facultatively anaerobic, short rod-shaped, non-motile,
Gram-stain-positive, unknown bacterial strains (JY-X040T
and JY-X174) were isolated from fluvial sediments of Tongtian
River in Yushu Tibetan Autonomous Prefecture, Qinghai
province, China. Cells formed translucent, gray, round and
convex colonies, with a diameter of less than 0.5 mm after 5
days of incubation at 30°C on brain heart infusion-5% sheep
blood agar. The 16S rRNA gene sequence similarity between
strain JY-X040T and Fudania jinshanensis 313T is 93.87%.
In the four phylogenetic trees constructed based on the 16S
rRNA gene and 423 core genes, the two isolates form an independent
branch, phylogenetically closest to F. jinshanensis
313T, but could not be classified as a member of the genus
Fudania or any other genus of the family Arcanobacteriaceae.
The DNA G + C content of strain JY-X040T was 57.8%. Calculation results of average nucleotide identity, digital DNADNA
hybridization value and amino acid identity between
strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%,
and 64.1%. The major cellular fatty acids were C16:0 (23%)
and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α
(L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol,
phosphatidylglycerol, phosphatidylinositol,
phosphatidylinositol mannoside and four unidentified components.
The whole-cell sugars contained rhamnose and ribose.
MK-10(H4) was the sole respiratory quinone. The minimum
inhibitory concentration of streptomycin was 32 μg/ml. All
physiological, biochemical, chemotaxonomic and genomic
characteristics support that strains JY-X040T and JY-X174
represent members of a novel species in a new genus, Changpingibacter
yushuensis gen. nov., sp. nov. The type strain is
JY-X040T (GDMCC 1.1996T = KCTC 49514T).