Journal of Microbiology

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Repeated Exposure of Vancomycin to Vancomycin-Susceptible Staphylococcus aureus (VSSA) Parent Emerged VISA and VRSA Strains with Enhanced Virulence Potentials: An Nguyen, J Jean Sophy Roy, Ji-Hoon Kim, Kyung-Hee Yun, Wonsik Lee, Kyeong Kyu Kim, Truc Kim, Akhilesh Kumar Chaurasia; J. Microbiol. 2024;62(7):535-553. Published online May 30, 2024; DOI: https://doi.org/10.1007/s12275-024-00139-8

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Abstract: The emergence of resistance against the last-resort antibiotic vancomycin in staphylococcal infections is a serious concern for human health. Although various drug-resistant pathogens of diverse genetic backgrounds show higher virulence potential, the underlying mechanism behind this is not yet clear due to variability in their genetic dispositions. In this study, we investigated the correlation between resistance and virulence in adaptively evolved isogenic strains. The vancomycin-susceptible Staphylococcus aureus USA300 was exposed to various concentrations of vancomycin repeatedly as a mimic of the clinical regimen to obtain mutation(s)-accrued-clonally-selected (MACS) strains. The phenotypic analyses followed by expression of the representative genes responsible for virulence and resistance of MACS strains were investigated. MACS strains obtained under 2 and 8 µg/ml vancomycin, named Van2 and Van8, respectively; showed enhanced vancomycin minimal inhibitory concentrations (MIC) to 4 and 16 µg/ml, respectively. The cell adhesion and invasion of MACS strains increased in proportion to their MICs. The correlation between resistance and virulence potential was partially explained by the differential expression of genes known to be involved in both virulence and resistance in MACS strains compared to parent S. aureus USA300. Repeated treatment of vancomycin against vancomycin-susceptible S. aureus (VSSA) leads to the emergence of vancomycin-resistant strains with variable levels of enhanced virulence potentials.

Nocardioides suum sp. nov. isolated from the air environment in an indoor pig farm: Siwon Lee , Wonseok Lee , Hyen-Mi Chung , Sangjung Park; J. Microbiol. 2017;55(6):417-420. Published online April 20, 2017; DOI: https://doi.org/10.1007/s12275-017-6313-y

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A bacterial strain PBT33-2T was isolated from the air environ-ment in an indoor pig farm. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PBT33-2T be-longed to the genus Nocardioides in the phylum Actinobac-teria, and was most closely related to Nocardioides daphnia D287T in a maximum-likelihood and neighbor-joining phy-logenetic trees. Strain PBT33-2T shared 95.3% sequence iden-tity with N. daphnia D287T. However, the highest sequence similarity was shown with N. sediminis MSL-01T (96.0%). It had less than 96.0% sequence identities with other type spe-cies of the genus Nocardioides. Strain PBT-33-2T grew at 15–45°C (optimum 20–35°C), pH 5.0–11.0 (optimum pH 7.0) and 0–4.0% (w/v) NaCl (optimum 0%). The major fatty acid and quinone were iso-C16:0 and MK-8, and the DNA G+C content of strain PBT33-2T was 69.3 mol%. On the basis of poly-phasic results, strain PBT33-2T represents a novel spe-cies of the genus Nocardioides, for which the name Nocar-dioides suum sp. nov. is proposed. Its type strain is PBT33-2T (=KCTC 39558T =DSM 102833T).

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Nocardioides cremeus sp. nov., Nocardioides abyssi sp. nov. and Nocardioides oceani sp. nov., three actinobacteria isolated from Western Pacific Ocean sediment
Wenjing Wang, Yihan Ding, Shiping Wei, Miaomiao Yin, Gaiyun Zhang
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Nocardioides humilatus sp. nov., isolated from farmland soil in the Republic of Korea
Jong Min Lee, Chun-Zhi Jin, Min-Kyoung Kang, So Hee Park, Dong-Jin Park, Dong-Gyun Kim, Chang-Jin Kim
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Description of Nocardioides piscis sp. nov., Sphingomonas piscis sp. nov. and Sphingomonas sinipercae sp. nov., isolated from the intestine of fish species Odontobutis interrupta (Korean spotted sleeper) and Siniperca scherzeri (leopard mandarin fish)
Dong-Wook Hyun, Yun-Seok Jeong, Jae-Yun Lee, Hojun Sung, So-Yeon Lee, Jee-Won Choi, Hyun Sik Kim, Pil Soo Kim, Jin-Woo Bae
Journal of Microbiology.2021; 59(6): 552. CrossRef
Nocardioides euryhalodurans sp. nov., Nocardioides seonyuensis sp. nov. and Nocardioides eburneiflavus sp. nov., isolated from soil
Su Gwon Roh, Chan Lee, Min-Kyeong Kim, Hye-Jeong Kang, Yeong Seok Kim, Min Ji Kim, Adeel Malik, Seung Bum Kim
International Journal of Systematic and Evolutionary Microbiology .2020; 70(4): 2682. CrossRef
Nocardioides speluncae sp. nov., a novel actinobacterium isolated from a karstic subterranean environment sample
Bao-Zhu Fang, Ming-Xian Han, Jian-Yu Jiao, Xiao-Tong Zhang, Yuan-Guo Xie, Wael N. Hozzein, Dalal Hussien M. Alkhalifah, Min Xiao, Wen-Jun Li
Antonie van Leeuwenhoek.2019; 112(6): 857. CrossRef

Nocardioides paucivorans sp. nov. Isolated from Soil: Jae-Hyung Ahn , Jun-Muk Lim , Soo-Jin Kim , Jaekyeong Song , Soon-Wo Kwon , Hang-Yeon Weon; J. Microbiol. 2014;52(12):990-994. Published online November 29, 2014; DOI: https://doi.org/10.1007/s12275-014-4587-x

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Abstract

One strain, designated KIS31-44T, was isolated from a soil sample collected from Dokdo Island, South Korea. The strain is Gram-stain-positive, aerobic, non-spore-forming and nonmotile. It grows optimally at 28–30°C, at pH 7.0 and 0% NaCl. 16S rRNA gene sequence analysis showed that strain KIS31-44T belonged to the genus Nocardioides and shared the highest sequence similarities with Nocardioides aestuarii JC2056T (95.5%) and Nocardioides terrae VA15T (95.0%). The major fatty acids of strain KIS31-44T were C17:1 ω6c, C18:1 ω9c, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), iso-C16:0, C18:0 10-methyl (TBSA), C16:0 2-OH, C17:0 10-methyl, and iso-C16:1 H. The major isoprenoid quinone was MK-8 (H4). The strain contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major polar lipids. The peptidoglycan structure was A3γ-type with LL-diaminopimelic acid. Based on these data, the isolate represents one novel species in the genus Nocardioides, for which the name Nocardioides paucivorans sp. nov. (type strain KIS31-44T =DSM 27142T =KACC 17309T) is proposed.

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Nocardioides alcanivorans sp. nov., a novel hexadecane-degrading species isolated from plastic waste
Julia Mitzscherling, Joana MacLean, Daniel Lipus, Alexander Bartholomäus, Kai Mangelsdorf, André Lipski, Vladimir Roddatis, Susanne Liebner, Dirk Wagner
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
Nocardioides sambongensis sp. nov., isolated from Dokdo Islands soil
Ye-Ji Hwang, Jin-Soo Son, Soo-Yeong Lee, Yuxi He, YoungJae Jo, Jae-Ho Shin, Sa-Youl Ghim
International Journal of Systematic and Evolutionary Microbiology .2020; 70(1): 16. CrossRef
Nocardioides speluncae sp. nov., a novel actinobacterium isolated from a karstic subterranean environment sample
Bao-Zhu Fang, Ming-Xian Han, Jian-Yu Jiao, Xiao-Tong Zhang, Yuan-Guo Xie, Wael N. Hozzein, Dalal Hussien M. Alkhalifah, Min Xiao, Wen-Jun Li
Antonie van Leeuwenhoek.2019; 112(6): 857. CrossRef
Nocardioides zhouii sp. nov., isolated from the Hailuogou Glacier in China
Guo-Qing Zhang, Qing Liu, Hong-Can Liu, Yu-Guang Zhou, Yu-Hua Xin
International Journal of Systematic and Evolutionary Microbiology.2019; 69(8): 2329. CrossRef
A strategy for securing unique microbial resources – focusing on Dokdo islands-derived microbial resources
Jong Myong Park, Ji Won Hong, Jin-Soo Son, Ye-Ji Hwang, Hyun-Min Cho, Young-Hyun You, Sa-Youl Ghim
Israel Journal of Ecology and Evolution.2018; 64(1-4): 1. CrossRef
Nocardioides cavernae sp. nov., an actinobacterium isolated from a karst cave
Ming-Xian Han, Bao-Zhu Fang, Ye Tian, Wan-Qin Zhang, Jian-Yu Jiao, Lan Liu, Zi-Tong Zhang, Min Xiao, Da-Qiao Wei, Wen-Jun Li
International Journal of Systematic and Evolutionary Microbiology .2017; 67(3): 633. CrossRef
Nocardioides thalensis sp. nov., isolated from a desert
Inam Ullah Khan, Firasat Hussain, Neeli Habib, Min Xiao, Iftikhar Ahmed, Arshia Amin, Xiao-Yang Zhi, Wen-Jun Li
International Journal of Systematic and Evolutionary Microbiology .2017; 67(8): 2848. CrossRef
Nocardioides baekrokdamisoli sp. nov., isolated from soil of a crater lake
Keun Chul Lee, Kwang Kyu Kim, Jong-Shik Kim, Dae-Shin Kim, Suk-Hyung Ko, Seung-Hoon Yang, Yong Kook Shin, Jung-Sook Lee
International Journal of Systematic and Evolutionary Microbiology.2016; 66(10): 4231. CrossRef
Nocardioides pakistanensis sp. nov., isolated from a hot water spring of Tatta Pani in Pakistan
Arshia Amin, Iftikhar Ahmed, Neeli Habib, Saira Abbas, Min Xiao, Wael N. Hozzein, Wen-Jun Li
Antonie van Leeuwenhoek.2016; 109(8): 1101. CrossRef
Nocardioides ungokensis sp. nov., isolated from lake sediment
Yan Zhao, Qingmei Liu, Myung-Suk Kang, Fengxie Jin, Hongshan Yu, Wan-Taek Im
International Journal of Systematic and Evolutionary Microbiology .2015; 65(Pt_12): 4857. CrossRef

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