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- Characteristic and role of chromosomal type II toxin-antitoxin systems locus in Enterococcus faecalis ATCC29212
- Zhen Li , Chao Shi , Shanjun Gao , Xiulei Zhang , Di Lu , Guangzhi Liu
- J. Microbiol. 2020;58(12):1027-1036. Published online October 23, 2020
- DOI: https://doi.org/10.1007/s12275-020-0079-3
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Abstract
- The Gram-positive bacterium Enterococcus faecalis is currently one of the major pathogens of nosocomial infections. The lifestyle of E. faecalis relies primarily on its remarkable capacity to face and survive in harsh environmental conditions. Toxin-antitoxin (TA) systems have been linked to the growth control of bacteria in response to adverse environments but have rarely been reported in Enterococcus. Three functional type II TA systems were identified among the 10 putative TA systems encoded by E. faecalis ATCC29212. These toxin genes have conserved domains homologous to MazF (DR75_ 1948) and ImmA/IrrE family metallo-endopeptidases (DR75_ 1673 and DR75_2160). Overexpression of toxin genes could inhibit the growth of Escherichia coli. However, the toxin DR75_1673 could not inhibit bacterial growth, and the bacteriostatic effect occurred only when it was coexpressed with the antitoxin DR75_1672. DR75_1948–DR75_1949 and DR75_ 160–DR75_2161 could maintain the stable inheritance of the unstable plasmid pLMO12102 in E. coli. Moreover, the transcription levels of these TAs showed significant differences when cultivated under normal conditions and with different temperatures, antibiotics, anaerobic agents and H2O2. When DR75_2161 was knocked out, the growth of the mutant strain at high temperature and oxidative stress was limited. The experimental characterization of these TAs loci might be helpful to investigate the key roles of type II TA systems in the physiology and environmental stress responses of Enterococcus.
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Citations
Citations to this article as recorded by
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BMC Oral Health.2024;[Epub] CrossRef - Unveiling the impact of antibiotic stress on biofilm formation and expression of toxin-antitoxin system genes in Clostridium difficile clinical isolates
Nasim Cheraghi, Saeed Khoshnood, Nourkhoda Sadeghifard, Niloufar Khodaei, Parisa Asadollahi, Saiyad Bastaminejad, Ebrahim Kouhsari, Nazanin Omidi, Behrooz Sadeghi Kalani
Molecular Biology Reports.2024;[Epub] CrossRef - Edwardsiella piscicida HigB: A type II toxin that is essential to oxidative resistance, biofilm formation, serum survival, intracellular propagation, and host infection
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Aquaculture.2021; 535: 736382. CrossRef
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