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- Fluorescence change of Fusobacterium nucleatum due to Porphyromonas gingivalis
- Min-Ah Lee , Si-Mook Kang , Se-Yeon Kim , Ji-Soo Kim , Jin-Bom Kim , Seung-Hwa Jeong
- J. Microbiol. 2018;56(9):628-633. Published online August 23, 2018
- DOI: https://doi.org/10.1007/s12275-018-7515-7
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Abstract
- The aim of this study was to measure changes in the fluorescence of Fusobacterium nucleatum interacting with Porphyromonas gingivalis for excitation with blue light at 405-nm. P. gingivalis was mono- and co-cultivated in close proximity with F. nucleatum. The fluorescence of the bacterial colonies was photographed using a QLF-D (Quantitative Light-induced Fluorescence-Digital) Biluminator camera system with a 405 nm light source and a specific filter. The red, green and blue intensities of fluorescence images were analyzed using the image analysis software. A fluorescence spectrometer was used to detect porphyrin synthesized by each bacterium. F. nucleatum, which emitted green fluorescence in single cultures, showed intense red fluorescence when it was grown in close proximity with P. gingivalis. F. nucleatum co-cultivated with P. gingivalis showed the same pattern of fluorescence peaks as for protoporphyrin IX in the red part of the spectrum. We conclude that the green fluorescence of F. nucleatum can change to red fluorescence in the presence of adjacent co-cultured with P. gingivalis, indicating that the fluorescence character of each bacterium might depend on the presence of other bacteria.
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Citations
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- Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light
Rafał Wiench, Dariusz Paliga, Anna Mertas, Elżbieta Bobela, Anna Kuśka-Kiełbratowska, Sonia Bordin-Aykroyd, Aleksandra Kawczyk-Krupka, Kinga Grzech-Leśniak, Monika Lukomska-Szymanska, Edward Lynch, Dariusz Skaba
Dentistry Journal.2024; 12(3): 48. CrossRef - Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Streptococcus mutans
Yung-Jhe Yan, Bo-Wen Wang, Chih-Man Yang, Ching-Yi Wu, Mang Ou-Yang
Dentistry Journal.2021; 9(7): 74. CrossRef - Fluorescence image and microbiological analysis of biofilm retained around healthy and inflamed orthodontic miniscrews
A.S. Garcez, L.C. Barros, M.R.U. Fernandes, D.N. Fujii, S.S. Suzuki, R. Nepomuceno
Photodiagnosis and Photodynamic Therapy.2020; 30: 101707. CrossRef
- Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light
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