Journal of Microbiology

Journal Article

Parabacteroides chongii sp. nov., isolated from blood of a patient with peritonitis: Hyunsoo Kim , Wan-Taek Im , Myungsook Kim , Dokyun Kim , Young Hee Seo , Dongeun Yong , Seok Hoon Jeong , Kyungwon Lee; J. Microbiol. 2018;56(10):722-726. Published online September 28, 2018; DOI: https://doi.org/10.1007/s12275-018-8122-3

Abstract: An obligate anaerobic, Gram-reaction-negative, non-sporeforming, non-motile, rod shaped bacterium designated YMC B3181T was isolated from the blood of a patient with peritonitis. Strain B3181T grew at 20 to 40°C with optimum growth at 37°C. 16S rRNA gene sequence similarity showed strain B3181T belongs to the genus Parabacteroides and is closely related to Parabacteroides faecis 157T (97.3%), Parabacteroides gordonii MS-1T (96.6%), and Parabacteroides goldsteinii DSM 19448T (95.7%). The G + C content of the genomic DNA was 42.3 mol%. The major cellular fatty acids were anteiso- C15:0 and iso-C17:0 3-OH, and the predominant respiratory quinones were MK-9 and MK-10 menaquinones. Genomic and chemotaxonomic data supported affiliation of B3181T with the genus Parabacteroides. Strain B3181T was phylogenetically and phenotypically different from recognized species of the genus Parabacteroides. Accordingly, this isolate belongs to a novel species for which the name Parabacteroides chongii sp. nov. (type strain YMC B3181T = LMG 30065T = KACC 19034T) is proposed.

Research Support, Non-U.S. Gov't

Genetic Introduction of Foreign Genes to Pleurotus eryngii by Restriction Enzyme-Mediated Integration: Won Noh , Sang-Woo Kim , Dong-Won Bae , Jae-Yean Kim , Hyeon-Su Ro; J. Microbiol. 2010;48(2):253-256. Published online May 1, 2010; DOI: https://doi.org/10.1007/s12275-010-9278-7

Abstract: Pleurotus eryngii was transformed via restriction enzyme-mediated integration. In order to construct the transformation plasmid, the enhanced cyan fluorescent protein (ECFP) gene was ligated next to the gpd promoter of the plasmid pAN7-1. Transformation was facilitated via the heat treatment of a transformation mixture containing 1 μg of the HindIII-digested plasmid DNA and 106 mushroom protoplasts in 40% polyethyleneglycol solution, resulting in 10-40 hygromycin-resistant transformants. Successful transformation was evidenced by PCR, Southern blot, and confocal fluorescence microscopic analyses on the selected transformants. To date, this is the first report on the transformation of P. eryngii by REMI technique.

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