Journal of Microbiology

Journal Articles

Variation of cassiicolin genes among Chinese isolates of Corynespora cassiicola: Jun Wu , Xuewen Xie , Yanxia Shi , Ali Chai , Qi Wang , Baoju Li; J. Microbiol. 2018;56(9):634-647. Published online July 27, 2018; DOI: https://doi.org/10.1007/s12275-018-7497-5

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Corynespora cassiicola is a species of fungus that is a plant pathogen of many agricultural crop plants, including severe target spot disease on cucumber. Cassiicolin is an important effector of pathogenicity of this fungus. In this study, we collected 141 Corynespora isolates from eighteen hosts, and the casscolin gene was detected in 82 C. cassiicola strains. The deduced protein sequences revealed that 72 isolates contained the Cas2 gene, two strains from Gynura bicolor harboured the Cas2.2 gene, and 59 isolates without a cassiicolin gene were classified as Cas0. Phylogenetic analyses was performed for the 141 isolates using four loci (ITS, ga4, caa5, and act1) and revealed two genetic clusters. Cluster A is composed of four subclades: subcluster A1 includes all Cas2 isolates plus 18 Cas0 strains, subcluster A2 includes the eight Cas5 isolates and one Cas0 isolate, and subclusters A3 and A4 contain Cas0 strains. Cluster B consists of 21 Cas0 isolates. Twenty-two C. cassiicola strains from different toxin classes showed varying degrees of virulence against cucumber. Cas0 or Cas2 strains induced diverse responses on cucumber, from no symptoms to symptoms of moderate or severe infection, but all Cas5 isolates exhibited avirulence on cucumber.

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Diversity of cassiicolin profiles and culture filtrate toxicity of Corynespora cassiicola isolates from South Indian rubber plantations
Reshma T R, Shilpa Babu, Vineeth V K, Shaji Philip
Industrial Crops and Products.2025; 224: 120243. CrossRef
The Diseases and Pests of Rubber Tree and Their Natural Control Potential: A Bibliometric Analysis
Liqiong Chen, Lidan Xu, Xiaona Li, Yilin Wang, Yun Feng, Guixiu Huang
Agronomy.2023; 13(8): 1965. CrossRef
Need for disease resistance breeding against Corynespora cassiicola in crops
Edgar Sierra-Orozco, German Sandoya, Seonghee Lee, Gary Vallad, Samuel Hutton
Frontiers in Agronomy.2023;[Epub] CrossRef
Comparison and Correlation of Corynespora cassiicola Populations from Kiwifruit and Other Hosts Based on Morphology, Phylogeny, and Pathogenicity
Jing Xu, Guoshu Gong, Yongliang Cui, Yuhang Zhu, Jun Wang, Kaikai Yao, Wen Chen, Cuiping Wu, Rui Yang, Xiaodan Yang, Pan Li, Henan Zhao, Sen Zhong, Yi Luo, Yue Li, Wenfei Liao
Plant Disease.2023; 107(7): 1979. CrossRef
Unraveling the Host-Selective Toxic Interaction of Cassiicolin with Lipid Membranes and Its Cytotoxicity
Kien Xuan Ngo, Phuong Doan N. Nguyen, Hirotoshi Furusho, Makoto Miyata, Tomomi Shimonaka, Nguyen Ngoc Bao Chau, Nguyen Phuong Vinh, Nguyen Anh Nghia, Tareg Omer Mohammed, Takehiko Ichikawa, Noriyuki Kodera, Hiroki Konno, Takeshi Fukuma, Nguyen Bao Quoc
Phytopathology®.2022; 112(7): 1524. CrossRef
The necrosis- and ethylene-inducing peptide 1-like protein (NLP) gene family of the plant pathogen Corynespora cassiicola
Thaís Carolina da Silva Dal’Sasso, Vinícius Delgado da Rocha, Hugo Vianna Silva Rody, Maximiller Dal-Bianco Lamas Costa, Luiz Orlando de Oliveira
Current Genetics.2022; 68(5-6): 645. CrossRef
Identification and virulence evaluation of Corynespora cassiicola cassiicolin-encoding gene isolates from rubber trees in Vietnam
Nguyen Ngoc Bao Chau, Nguyen Van Minh, Nguyen Mai Nghiep, Nguyen Phuong Vinh, Nguyen Anh Nghia, Nguyen Bao Quoc
Tropical Plant Pathology.2022; 47(3): 378. CrossRef
The fungal pathogen Corynespora cassiicola: A review and insights for target spot management on cotton and Soya bean
Marina N. Rondon, Kathy Lawrence
Journal of Phytopathology.2021; 169(6): 329. CrossRef
Mitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen Corynespora cassiicola
Qingzhou Ma, Haiyan Wu, Yuehua Geng, Qiang Li, Rui Zang, Yashuang Guo, Chao Xu, Meng Zhang
Computational and Structural Biotechnology Journal.2021; 19: 5987. CrossRef
Genomic Characteristics and Comparative Genomics Analysis of Two Chinese Corynespora cassiicola Strains Causing Corynespora Leaf Fall (CLF) Disease
Boxun Li, Yang Yang, Jimiao Cai, Xianbao Liu, Tao Shi, Chaoping Li, Yipeng Chen, Pan Xu, Guixiu Huang
Journal of Fungi.2021; 7(6): 485. CrossRef
Endophytes from Wild Rubber Trees as Antagonists of the Pathogen Corynespora cassiicola
Valérie Pujade-Renaud, Marine Déon, Romina Gazis, Sébastien Ribeiro, Florence Dessailly, Françoise Granet, Priscila Chaverri
Phytopathology®.2019; 109(11): 1888. CrossRef

Biosynthesis of 2-amino-3-hydroxycyclopent-2-enone moiety of bafilomycin in Kitasatospora cheerisanensis KCTC2395: Nguyen Phan Kieu Hanh , Jae Yoon Hwang , Hye Ryeung Oh , Geum Jin Kim , Hyukjae Choi , Doo Hyun Nam; J. Microbiol. 2018;56(8):571-578. Published online July 25, 2018; DOI: https://doi.org/10.1007/s12275-018-8267-0

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Abstract

Bafilomycins produced by Kitasatospora cheerisanensis KCTC- 2395 belong to the 16-membered macrolactone family plecomacrolide antibiotics. Bafilomycin B1 contains 2-amino- 3-hydroxycyclopent-2-enone (C5N), a five membered ring, which gets condensed via an amide linkage to bafilomycin polyketide. To study the biosynthetic pathway of C5N during bafilomycin biosynthesis in K. cheerisanensis KCTC2395, we attempted the functional analysis of two putative genes, encoding 5-aminolevulinic acid synthase (ALAS) and acyl- CoA ligase (ACL). The amplified putative genes for ALAS and ACL were cloned into the E. coli expression vector pET- 32a(+) plasmid, following which the soluble recombinant ALAS and ACL proteins were purified through nickel-affinity column chromatography. Through HPLC analysis of the enzyme reaction mixture, we confirmed the products of putative ALAS and ACL reaction as 5-aminolevulinic acid (5- ALA) and 5-ALA-CoA, respectively. The optimal pH for the putative ALAS reaction was 7.5, and for putative ACL reaction was 7.0, as confirmed by the colorimetric assay. Furthermore, pyridoxal 5􍿁-phosphate (PLP) was found to be an essential cofactor in the putative ALAS reaction, and ATP was a cofactor for the putative ACL catalysis. Finally, we also confirmed that the simultaneous treatment of putative ACL and putative ALAS enzymes resulted in the production of C5N compound from 5-ALA.

Citations

Citations to this article as recorded by

The Secondary Metabolites from Genus Kitasatospora: A Promising Source for Drug Discovery
Yuanjuan Wei, Guiyang Wang, Yan Li, Maoluo Gan
Chemistry & Biodiversity.2024;[Epub] CrossRef
Elucidation of the Late Steps during Hexacosalactone A Biosynthesis in Streptomyces samsunensis OUCT16-12
He Duan, Fang Wang, Chuchu Zhang, Yujing Dong, Huayue Li, Fei Xiao, Wenli Li, Haruyuki Atomi
Applied and Environmental Microbiology.2023;[Epub] CrossRef
A Secondary Metabolic Enzyme Functioned as an Evolutionary Seed of a Primary Metabolic Enzyme
Jun Kawaguchi, Hikaru Mori, Noritaka Iwai, Masaaki Wachi, Miriam Barlow
Molecular Biology and Evolution.2022;[Epub] CrossRef
Biosynthesis of Methoxymalonyl-acyl Carrier Protein (ACP) as an Extender Unit for Bafilomycin Polyketide in Streptomyces griseus DSM 2608
Nguyen Phan Kieu Hanh, Jae Yoon Hwang, Doo Hyun Nam
Biotechnology and Bioprocess Engineering.2018; 23(6): 693. CrossRef

Research Support, Non-U.S. Gov't

An Improved Method for Extracting Bacteria from Soil for High Molecular Weight DNA Recovery and BAC Library Construction: Juan Liu , Jingquan Li , Li Feng , Hui Cao , Zhongli Cui; J. Microbiol. 2010;48(6):728-733. Published online January 9, 2011; DOI: https://doi.org/10.1007/s12275-010-0139-1

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Abstract: Separation of bacterial cells from soil is a key step in the construction of metagenomic BAC libraries with large DNA inserts. Our results showed that when combined with sodium pyro-phosphate and homogenization for soil dispersion, sucrose density gradient centrifugation (SDGC) was more effective at separating bacteria from soil than was low speed centrifugation (LSC). More than 70% of the cells, along with some soil colloids, were recovered with one round of centrifugation. A solution of 0.8% NaCl was used to resuspend these cell and soil pellets for purification with nycodenz density gradient centrifugation (NDGC). After purification, more than 30% of the bacterial cells in the primary soil were extracted. This procedure effectively removed soil contamination and yielded sufficient cells for high molecular weight (HMW) DNA isolation. Ribosomal intergenic spacer analysis (RISA) showed that the microbial community structure of the extracted cells was similar to that of the primary soil, suggesting that this extraction procedure did not significantly change the the soil bacteria community structure. HMW DNA was isolated from bacterial cells extracted from red soil for metagenomic BAC library construction. This library contained DNA inserts of more than 200 Mb with an average size of 75 kb.

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