Journal of Microbiology

Research Support, Non-U.S. Gov't

NOTE] Improved Antibiotic Resistance Gene Cassette for Marker Exchange Mutagenesis in Ralstonia solanacearum and Burkholderia Species: Hae Young Um , Eunsook Chung , Jai-Heon Lee , Seon-Woo Lee; J. Microbiol. 2011;49(2):305-308. Published online May 3, 2011; DOI: https://doi.org/10.1007/s12275-011-0439-0

Abstract: Marker exchange mutagenesis is a fundamental approach to understanding gene function at a molecular level in bacteria. New plasmids carrying a kanamycin resistance gene or a trimethoprim resistance gene were constructed to provide antibiotic resistance cassettes for marker exchange mutagenesis in Ralstonia solanacearum and many antibiotic-resistant Burkholderia spp. Insertion sequences present in the flanking sequences of the antibiotic resistance cassette were removed to prevent aberrant gene replacement and polar mutation during mutagenesis in wild-type bacteria. Plasmids provided in this study would be convenient for use in gene cassettes for gene replacement in other Gram-negative bacteria.

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