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- Complete genetic dissection and cell type-specific replication of old world alphaviruses, getah virus (GETV) and sagiyama virus (SAGV)
- Yiwen Zhang , Jinhan Yu , Lu Tan , Xingxing Wang , Runsheng Li , Dal Young Kim
- J. Microbiol. 2021;59(11):1044-1055. Published online September 27, 2021
- DOI: https://doi.org/10.1007/s12275-021-1361-8
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Abstract
- Getah virus (GETV), which was first isolated in Malaysia in 1955, and Sagiyama virus (SAGV), isolated in Japan in 1956, are members of the genus Alphavirus in the family Togaviridae. It is a consensus view that SAGV is a variant of GETV. In the present study, we determined the complete sequences of the prototype GETV MM2021 and SAGV M6-Mag132 genomic RNA extracted from plaque-purified viruses. The MM2021 genome was 11,692 nucleotides (nt) in length in the absence of 3poly(A) tail, and the length of M6-Mag132 genome was 11,698 nt. Through sequence alignment of MM2021 and M6-Mag132, we located all the amino acid differences between these two strains, which were scattered in all the encoded proteins. Subsequently, we validated the close evolutionary relationship between GETV and SAGV by constructing phylogenetic trees based on either complete genomes or structural genomes. We eventually analyzed the growth kinetics of GETV and SAGV as well as other representative alphaviruses in various mammalian and insect cell lines. It was shown that human-oriented cell lines such as HEK-293T and Hela cells were relatively resistant to GETV and SAGV infection due to absence of proviral factors or species-specific barrier. On the other hand, both GETV and SAGV replicated efficiently in non-human cell lines. Our results provide essential genetic information for future epidemiological surveillance on Alphaviruses and lay the foundation for developing effective interventions against GETV and SAGV.
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Citations
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- Novel Porcine Getah Virus from Diarrheal Piglets in Jiangxi Province, China: Prevalence, Genome Sequence, and Pathogenicity
Jianhui Lan, Mengtao Fang, Leilei Duan, Zhong Liu, Guanggao Wang, Qi Wu, Ke Fan, Dongyan Huang, Yu Ye, Gen Wan, Yuxin Tang, Deping Song
Animals.2024; 14(20): 2980. CrossRef - Characterization and arbovirus susceptibility of cultured CERNI cells derived from sika deer (Cervus nippon)
Makoto Takeishi, Shigeru Morikawa, Ryusei Kuwata, Mitsumori Kawaminami, Hiroshi Shimoda, Haruhiko Isawa, Ken Maeda, Yasuhiro Yoshikawa
In Vitro Cellular & Developmental Biology - Animal.2024; 60(8): 935. CrossRef - Getah Virus Infection Rapidly Causes Testicular Damage and Decreases Sperm Quality in Male Mice
Fengqing Li, Bing Zhang, Zhiwen Xu, Chaoyuan Jiang, Mincai Nei, Lei Xu, Jun Zhao, Huidan Deng, Xiangang Sun, Yuancheng Zhou, Ling Zhu
Frontiers in Veterinary Science.2022;[Epub] CrossRef
- Novel Porcine Getah Virus from Diarrheal Piglets in Jiangxi Province, China: Prevalence, Genome Sequence, and Pathogenicity
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