MSK
Search
- Page Path
- HOME > Search
Review
- MINIREVIEW] Modern and Simple Construction of Plasmid: Saving Time and Cost
- Hideki Nakayama , Nobuo Shimamoto
- J. Microbiol. 2014;52(11):891-897. Published online October 31, 2014
- DOI: https://doi.org/10.1007/s12275-014-4501-6
- 53 View
- 0 Download
- 7 Crossref
-
Abstract
- Construction of plasmids has been occupying a significant fraction of laboratory work in most fields of experimental biology. Tremendous effort was made to improve the traditional method for constructing plasmids, in which DNA fragments digested with restriction enzymes were ligated. However, the traditional method remained to be a standard protocol more than 40 years. At last, several recent inventions are rapidly and completely replacing the traditional method, because they are far quicker with less cost, and requiring less material. We here introduce three such methods that cover up most of the cases. Moreover, they are complementary with each other. Our lab protocols are provided for “no strain, no pain” construction of plasmids.
-
Citations
Citations to this article as recorded by
- Tumor biology experimental course design based on the integration of molecular biology and metabolomics
Xinliang Zhu, Ting Tang
Cogent Education.2024;[Epub] CrossRef - Truncation-Free Genetic Code Expansion with Tetrazine Amino Acids for Quantitative Protein Ligations
Alex J. Eddins, Riley M. Bednar, Subhashis Jana, Abigail H. Pung, Lea Mbengi, Kyle Meyer, John J. Perona, Richard B. Cooley, P. Andrew Karplus, Ryan A. Mehl
Bioconjugate Chemistry.2023; 34(12): 2243. CrossRef - Involvement of GcvB small RNA in intrinsic resistance to multiple aminoglycoside antibiotics in Escherichia coli
Akira Muto, Simon Goto, Daisuke Kurita, Chisato Ushida, Hyota Himeno
The Journal of Biochemistry.2021; 169(4): 485. CrossRef - Flagellum-mediated motility in Pelotomaculum thermopropionicum SI
Tomoyuki Kosaka, Mutsumi Goda, Manami Inoue, Toshiharu Yakushi, Mamoru Yamada
Bioscience, Biotechnology, and Biochemistry.2019; 83(7): 1362. CrossRef - Natural killer cells unleashed: Checkpoint receptor blockade and BiKE/TriKE utilization in NK-mediated anti-tumor immunotherapy
Zachary B. Davis, Daniel A. Vallera, Jeffrey S. Miller, Martin Felices
Seminars in Immunology.2017; 31: 64. CrossRef - In vivo Assembly in Escherichia coli of Transformation Vectors for Plastid Genome Engineering
Yuyong Wu, Lili You, Shengchun Li, Meiqi Ma, Mengting Wu, Lixin Ma, Ralph Bock, Ling Chang, Jiang Zhang
Frontiers in Plant Science.2017;[Epub] CrossRef - Role of 100S ribosomes in bacterial decay period
Ksenia Shcherbakova, Hideki Nakayama, Nobuo Shimamoto
Genes to Cells.2015; 20(10): 789. CrossRef
- Tumor biology experimental course design based on the integration of molecular biology and metabolomics
First
Prev
TOP
