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- The NADP+-dependent glutamate dehydrogenase Gdh1 is subjected to glucose starvation-induced reversible aggregation that affects stress resistance in yeast
- Woo Hyun Lee , Ju Yeong Oh , Pil Jae Maeng
- J. Microbiol. 2019;57(10):884-892. Published online August 3, 2019
- DOI: https://doi.org/10.1007/s12275-019-9065-z
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- 3 Web of Science
- 2 Crossref
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Abstract
- The yeast Saccharomyces cerevisiae has two isoforms of NADP+-dependent glutamate dehydrogenase (Gdh1 and Gdh3) that catalyze the synthesis of glutamate from α-ketoglutarate and NH4 +. In the present study, we confirmed that Gdh3, but not Gdh1, mainly contributes to the oxidative stress resistance of stationary-phase cells and found evidence suggesting that the insignificance of Gdh1 to stress resistance is possibly resulted from conditional and reversible aggregation of Gdh1 into punctuate foci initiated in parallel with postdiauxic growth. Altered localization to the mitochondria or peroxisomes prevented Gdh1, which was originally localized in the cytoplasm, from stationary phase-specific aggregation, suggesting that some cytosolic factors are involved in the process of Gdh1 aggregation. Glucose starvation triggered the transition of the soluble form of Gdh1 into the insoluble aggregate form, which could be redissolved by replenishing glucose, without any requirement for protein synthesis. Mutational analysis showed that the N-terminal proximal region of Gdh1 (NTP1, aa 21-26, TLFEQH) is essential for glucose starvation-induced aggregation. We also found that the substitution of NTP1 with the corresponding region of Gdh3 (NTP3) significantly increased the contribution of the mutant Gdh1 to the stress resistance of stationary-phase cells. Thus, this suggests that NTP1 is responsible for the negligible role of Gdh1 in maintaining the oxidative stress resistance of stationary- phase cells and the stationary phase-specific stresssensitive phenotype of the mutants lacking Gdh3.
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Citations
Citations to this article as recorded by
- Genomic characterization of denitrifying methylotrophic Pseudomonas aeruginosa strain AAK/M5 isolated from municipal solid waste landfill soil
Ashish Kumar Singh, Rakesh Kumar Gupta, Hemant J. Purohit, Anshuman Arun Khardenavis
World Journal of Microbiology and Biotechnology.2022;[Epub] CrossRef - Effects of Molecular Crowding and Betaine on HSPB5 Interactions, with Target Proteins Differing in the Quaternary Structure and Aggregation Mechanism
Vera A. Borzova, Svetlana G. Roman, Anastasiya V. Pivovarova, Natalia A. Chebotareva
International Journal of Molecular Sciences.2022; 23(23): 15392. CrossRef
- Genomic characterization of denitrifying methylotrophic Pseudomonas aeruginosa strain AAK/M5 isolated from municipal solid waste landfill soil
- HST1 increases replicative lifespan of a sir2Δ mutant in the absence of PDE2 in Saccharomyces cerevisiae
- Woo Kyu Kang , Mayur Devare , Jeong-Yoon Kim
- J. Microbiol. 2017;55(2):123-129. Published online January 26, 2017
- DOI: https://doi.org/10.1007/s12275-017-6535-z
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- 4 Crossref
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Abstract
- Silent information regulator 2 (Sir2), which is the founding member of the sirtuin family of proteins, is a pro-longevity factor for replicative lifespan (RLS) in Saccharomyces cerevisiae. Sir2 is required for transcriptional silencing at mating type loci, telomeres, and rDNA loci. Sir2 also represses transcription of highly expressed growth-related genes, such as PMA1 and some ribosomal protein genes. Although the Sir2 paralogues Hst1, Hst2, Hst3, and Hst4 occur in S. cerevisiae, none of them could replace the transcriptional regulation of PMA1 by Sir2 in the wild type. In this study, we demonstrate that Hst1, the closest Sir2 paralogue, deacetylates the acetylated lysine 16 of histone H4 (H4K16Ac) and represses PMA1 transcription in the sir2Δ pde2Δ mutant. We further show that Hst1 plays a role in extending the RLS of the sir2Δ pde2Δ mutant. Collectively, our results suggest that Hst1 can substitute for Sir2 by deacetylating H4K16Ac only in the sir2Δ pde2Δ.
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Citations
Citations to this article as recorded by- Selective production, relationship and controversy between Monascus pigments and citrinin
Xueling Qin, Bin Xie, Xuanli Zong, Xiang Yu, Yanli Feng
Food Bioscience.2023; 56: 103233. CrossRef - Gene repression in S. cerevisiae—looking beyond Sir-dependent gene silencing
Safia Mahabub Sauty, Kholoud Shaban, Krassimir Yankulov
Current Genetics.2021; 67(1): 3. CrossRef - Characterization of Resveratrol, Oxyresveratrol, Piceatannol and Roflumilast as Modulators of Phosphodiesterase Activity. Study of Yeast Lifespan
Adrián Matencio, Francisco García-Carmona, José Manuel López-Nicolás
Pharmaceuticals.2020; 13(9): 225. CrossRef - NAD+-dependent HDAC inhibitor stimulates Monascus pigment production but inhibit citrinin
Yan Hu, Youxiang Zhou, Zejing Mao, Huihui Li, Fusheng Chen, Yanchun Shao
AMB Express.2017;[Epub] CrossRef
- Selective production, relationship and controversy between Monascus pigments and citrinin
Review
- MINIREVIEW] The Potential Hazards of Aspergillus sp. in Foods and Feeds, and the Role of Biological Treatment: A Review
- Sheikh Imranudin Sheikh-Ali , Akil Ahmad , Siti-Hamidah Mohd-Setapar , Zainul Akmal Zakaria , Norfahana Abdul-Talib , Aidee Kamal Khamis , Md Enamul Hoque
- J. Microbiol. 2014;52(10):807-818. Published online October 1, 2014
- DOI: https://doi.org/10.1007/s12275-014-4294-7
- 59 View
- 0 Download
- 16 Crossref
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Abstract
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The contamination of food and feed by Aspergillus has become
a global issue with a significant worldwide economic
impact. The growth of Aspergillus is unfavourable to the
development of food and feed industries, where the problems
happen mostly due to the presence of mycotoxins, which is a
toxic metabolite secreted by most Aspergillus groups. Moreover,
fungi can produce spores that cause diseases, such as
allergies and asthma, especially to human beings. High temperature,
high moisture, retarded crops, and poor food storage
conditions encourage the growth of mold, as well as
the development of mycotoxins. A variety of chemical, biological,
and physical strategies have been developed to control
the production of mycotoxins. A biological approach,
using a mixed culture comprised of Saccharomyces cerevisiae
and Lactobacillus rhamnosus resulted in the inhibition of the
growth of fungi when inoculated into fermented food. The
results
reveal that the mixed culture has a higher potential (37.08%) to inhibit the growth of Aspergillus flavus (producer of Aflatoxin) compared to either single culture, L. rhamnosus NRRL B-442 and S. cerevisiae, which inhibit the growth by 63.07% and 64.24%, respectively. -
Citations
Citations to this article as recorded by- Development of a reusable polymeric fluorescence sensor based on acryloyl β-cyclodextrin for the determination of aflatoxin B1 in grain products
Soner Çubuk, Neşe Taşci, Sümeyra Kalyoncu, Ece Kök Yetimoğlu, Memet Vezir Kahraman
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy.2025; 324: 124965. CrossRef - Synthesis and biological evaluation of titanium dioxide/thiopolyurethane composite: anticancer and antibacterial effects
Rana R. El Sadda, Mai S. Eissa, Rokaya K. Elafndi, Elhossein A. Moawed, Mohamed M. El-Zahed, Hoda R. Saad
BMC Chemistry.2024;[Epub] CrossRef - The association of physical development density, prevalence and types of microbes in colonized façade finishes in Enugu metropolis, Nigeria
Lawrence A. Isiofia, Emmanuel Nna, Francis O. Uzuegbunam, Eziyi O. Ibem
International Journal of Building Pathology and Adaptation.2024; 42(6): 1147. CrossRef - Anti-inflammatory potential of mycoprotein peptides obtained from fermentation of Schizophyllum commune DS1 with young apples
Zhengmei Ji, Wenjun Ma, Pengfei Liang, Xiaoyu Wang, Shuai Zhang, Yanhui Han, Yurong Guo
International Journal of Biological Macromolecules.2024; 281: 136638. CrossRef - In-situ synthesis of sepiolite-supported ceria nanocrystal composites for efficient removal of aflatoxin B1: Enhanced degradation of mycotoxins in the environment by sepiolite nanofibers
Na Zhang, Ningxi Li, Xiaoyu Han, Hong Zhang, Junping Meng, Pengfei Zhou, Jinsheng Liang
Journal of Alloys and Compounds.2023; 960: 170800. CrossRef - High-throughput and point-of-care detection of wheat fungal diseases: Potentialities of molecular and phenomics techniques toward in-field applicability
Sara Francesconi
Frontiers in Agronomy.2022;[Epub] CrossRef - Construction of a dual-functional CuO/BiOCl heterojunction for high-efficiently photoelectrochemical biosensing and photoelectrocatalytic degradation of aflatoxin B1
Lebao Mao, Hao Liu, Linli Yao, Wei Wen, Miao-Miao Chen, Xiuhua Zhang, Shengfu Wang
Chemical Engineering Journal.2022; 429: 132297. CrossRef - Healthy Zerumbone: From Natural Sources to Strategies to Improve Its Bioavailability and Oral Administration
María Dolores Ibáñez, Noelia M. Sánchez-Ballester, María Amparo Blázquez
Plants.2022; 12(1): 5. CrossRef - Coumarin derivative, 5′-hydroxy-auraptene, extracted from Lotus lalambensis, displays antifungal and anti-aflatoxigenic activities against Aspergillus flavus
Enas M. Ali, Mayyadah A. Alkuwayti, Munirah F. Aldayel, Basem M. Abdallah
Journal of King Saud University - Science.2021; 33(1): 101216. CrossRef - Aspergillus Metabolome Database for Mass Spectrometry Metabolomics
Alberto Gil-de-la-Fuente, Maricruz Mamani-Huanca, María C. Stroe, Sergio Saugar, Alejandra Garcia-Alvarez, Axel A. Brakhage, Coral Barbas, Abraham Otero
Journal of Fungi.2021; 7(5): 387. CrossRef - Behaviour of Aspergillus parasiticus in aflatoxin production as influenced by storage parameters using response surface methodology approach
Stephen Abiola Akinola, Collins Njie Ateba, Mulunda Mwanza
International Journal of Food Microbiology.2021; 357: 109369. CrossRef - Updates on the Functions and Molecular Mechanisms of the Genes Involved in Aspergillus flavus Development and Biosynthesis of Aflatoxins
Elisabeth Tumukunde, Rui Xie, Shihua Wang
Journal of Fungi.2021; 7(8): 666. CrossRef - Essential oils from the genus Thymus as antimicrobial food preservatives: Progress in their use as nanoemulsions-a new paradigm
Abhay K. Pandey, Mónica L. Chávez-González, Ana Sanches Silva, Pooja Singh
Trends in Food Science & Technology.2021; 111: 426. CrossRef - Photocatalytic degradation of aflatoxin B1 by activated carbon supported TiO2 catalyst
Shumin Sun, Ran Zhao, Yanli Xie, Yong Liu
Food Control.2019; 100: 183. CrossRef - Selected essential oil vapours inhibit growth of Aspergillus spp. in oats with improved consumer acceptability
Matěj Božik, Miroslava Císarová, Dana Tančinová, Lenka Kouřimská, Lukáš Hleba, Pavel Klouček
Industrial Crops and Products.2017; 98: 146. CrossRef - Reduction of aflatoxin B1 in peanut meal by extrusion cooking
Haiyan Zheng, Shuai Wei, Ying Xu, Mingtao Fan
LWT - Food Science and Technology.2015; 64(2): 515. CrossRef
- Development of a reusable polymeric fluorescence sensor based on acryloyl β-cyclodextrin for the determination of aflatoxin B1 in grain products
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