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- Characterization of Marinilongibacter aquaticus gen. nov., sp. nov., a unique marine bacterium harboring four CRISPR-Cas systems in the phylum Bacteroidota
- Dao-Feng Zhang , Yu-Fang Yao , Hua-Peng Xue , Zi-Yue Fu , Xiao-Mei Zhang , Zongze Shao
- J. Microbiol. 2022;60(9):905-915. Published online August 1, 2022
- DOI: https://doi.org/10.1007/s12275-022-2102-3
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- 5 Web of Science
- 6 Crossref
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Abstract
PDF - A novel bacterium, designated YYF0007T, was isolated from an agar-degrading co-culture. The strain was found harboring four CRISPR-Cas systems of two classes in the chromosome and subsequently subjected to a study on polyphasic taxonomy. Pairwise analyses of the 16S rRNA gene sequences indicated that strain YYF0007T had highest 16S rRNA gene sequence similarity (92.2%) to Jiulongibacter sediminis JN- 14-9T. The phylogenomic trees based on the 16S rRNA gene and 269 single-copy orthologous gene clusters (OCs) indicated that strain YYF0007T should be recognized as a novel genus of the family Spirosomaceae. The cells were Gramstain- negative, nonmotile, strictly aerobic, and straight long rods with no flagellum. Optimum growth occurred at 28°C and pH 7.0 with the presence of NaCl concentration 1.0–3.0% (w/v). The strain showed oxidase and catalase activities. The major fatty acids were C16:1ω5c, iso-C15:0 and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The predominant isoprenoid quinone was MK-7. The complete genome size was 4.64 Mb with a DNA G + C content of 44.4%. Further typing of CRISPR-Cas systems in the family Spirosomaceae and the phylum Bacteroidota indicated that it was remarkable for strain YYF0007T featured by such a set of CRISPR-Cas systems. This trait highlights the applications of strain YYF- 0007T in studies on the evolutionary dynamics and bacterial autoimmunity of CRISPR-Cas system as a potential model. The name Marinilongibacter aquaticus gen. nov., sp. nov. is proposed, and the type strain is YYF0007T (= MCCC 1K06017T = GDMCC 1.2428T = JCM 34683T).
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Citations
Citations to this article as recorded by
- Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects
Jiashun Li, Shuaishuai Wu, Kaidian Zhang, Xueqiong Sun, Wenwen Lin, Cong Wang, Senjie Lin
Microorganisms.2024; 12(1): 118. CrossRef - Unraveling the mechanisms behind sodium persulphate-induced changes in petroleum-contaminated aquifers’ biogeochemical parameters and microbial communities
Yuqi Qi, Jun Zeng, Junshi Tao, Rentao Liu, Renchuan Fu, Chao Yan, Xiao Liu, Na Liu, Yanru Hao
Chemosphere.2024; 351: 141174. CrossRef -
Arcicella gelida sp. nov. and Arcicella lustrica sp. nov., isolated from streams in China and re-examining the taxonomic status of all the genera within the families Spirosomataceae and Cytophagaceae
Huibin Lu, Li Chen, Linpei Huang
International Journal of Systematic and Evolutionary Microbiology .2024;[Epub] CrossRef - Thalassospira aquimaris sp. nov. and Winogradskyella marincola sp. nov. two marine bacteria isolated from an agar-degrading co-culture
Zi-Yue Fu, Dao-Feng Zhang, Meng-Han Huang, Hong-Chuan Wang, Xiao-Ye Chen, Yu-Fang Yao, Yang Yuan, Wen-Jun Li
Antonie van Leeuwenhoek.2024;[Epub] CrossRef - Validation List no. 209. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef - Telluribacter roseus sp. nov., Isolated from the Kumtag Desert Soil
Chu-Ying Feng, Jia-Rui Han, Chun-Yan Lu, Li Gu, Shuai Li, Wen-Hui Lian, Lei Dong, Wen-Jun Li
Current Microbiology.2023;[Epub] CrossRef
- Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-Associated Protein and Its Utility All at Sea: Status, Challenges, and Prospects
- Gut microbiota metabolic characteristics in coronary artery disease patients with hyperhomocysteine
- Ran Tian , Hong-Hong Liu , Si-Qin Feng , Yi-Fei Wang , Yi-Yang Wang , Yu-Xiong Chen , Hui Wang , Shu-Yang Zhang
- J. Microbiol. 2022;60(4):419-428. Published online March 4, 2022
- DOI: https://doi.org/10.1007/s12275-022-1451-2
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- 12 Web of Science
- 10 Crossref
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Abstract
PDF
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Hyperhomocysteine (HHcy) is known as a risk factor for coronary
artery disease (CAD). Despite the knowledge that gut
microbiota related metabolism pathway shares metabolites
with that of Hcy, little has been shown concerning the association
between HHcy and gut microbiota. To explore their
relationship in the context of CAD, 105 patients and 14 healthy
controls were recruited from one single medical center located
in Beijing, China. Their serum and fecal samples were
collected, with multi-omics analyses performed via LC/MS/
MS and 16S rRNA gene V3-V4 region sequencing, respectively.
Participants from the prospective cohort were divided
into CAD, CAD & HHcy and healthy controls (HC) groups
based on the diagnosis and serum Hcy concentration. The
results
revealed significant different metabolic signatures between CAD and CAD & HHcy groups. CAD patients with HHcy suffered a heavier atherosclerotic burden compared to CAD patients, and the difference was closely associated to betaine-homocysteine S-methyltransferase (BHMT)-related metabolites and trimethylamine N-oxide (TMAO)-related metabolites. Dimethylglycine (DMG) exhibited a strong positive correlation with serum total Hcy (tHcy), and TMAO and trimethylysine (TML) were associated with heavier atherosclerotic burden. Multiple other metabolites were also identified to be related to distinct cardiovascular risk factors. Additionally, Clostridium cluster IV and Butyricimonas were enriched in CAD patients with elevated tHcy. Our study suggested that CAD patients with elevated tHcy were correlated with higher atherosclerotic burden, and the impaired Hcy metabolism and cardiovascular risk were closely associated with BHMT-related metabolites, TMAO-related metabolites and impaired gut microbiota homeostasis. -
Citations
Citations to this article as recorded by- Homocysteine, Nutrition, and Gut Microbiota: A Comprehensive Review of Current Evidence and Insights
Deborah Agostini, Alessia Bartolacci, Rossella Rotondo, Maria Francesca De Pandis, Michela Battistelli, Matteo Micucci, Lucia Potenza, Emanuela Polidori, Fabio Ferrini, Davide Sisti, Francesco Pegreffi, Valerio Pazienza, Edy Virgili, Vilberto Stocchi, Sab
Nutrients.2025; 17(8): 1325. CrossRef - Serum homocysteine showed potential association with cognition and abnormal gut microbiome in major depressive disorder
Chen-Chen Xu, Wen-Xuan Zhao, Yu Sheng, Ya-Jun Yun, Ting Ma, Ning Fan, Jia-Qi Song, Jun Wang, Qi Zhang
World Journal of Psychiatry.2025;[Epub] CrossRef - Novel opportunity of treatment for psycho-cardiologic disease by gut microbiome
Yurui Lai, Chenli Fang, Yuang Jiang, Chengying Yang, Qiao Zhou, Yihua Cai, Yan Wei, Xinrong Fan
Frontiers in Cardiovascular Medicine.2025;[Epub] CrossRef - Nicotinamide N-methyltransferase in cardiovascular Diseases: Mechanistic insights and therapeutic potential
Shuang-Zhou Bi, Wei-Dong Sun, Xiao-Juan Zhu, Shi-Yan Lai, An-Liu, Chen-Ying Zhang, Jiang-Hua Li
European Journal of Medicinal Chemistry.2025; 295: 117790. CrossRef - Contribution of dietary methionine to gut health and its related diseases: implications for precision nutrition
Yuhui Yang, Yanli Xie, Manman Lu, Yuncong Xu, Renyong Zhao
Critical Reviews in Food Science and Nutrition.2025; : 1. CrossRef - Unravelling the Gut Microbiome Role in Cardiovascular Disease: A Systematic Review and a Meta-Analysis
Diana Martins, Cláudia Silva, António Carlos Ferreira, Sara Dourado, Ana Albuquerque, Francisca Saraiva, Ana Beatriz Batista, Pedro Castro, Adelino Leite-Moreira, António S. Barros, Isabel M. Miranda
Biomolecules.2024; 14(6): 731. CrossRef - Gut Commensal Bacteroides thetaiotaomicron Promote Atherothrombosis via Regulating L-Tryptophan Metabolism
Honghong Liu, Siqin Feng, Muyun Tang, Ran Tian, Shuyang Zhang
Reviews in Cardiovascular Medicine.2024;[Epub] CrossRef - Relation between homocysteine-to-adropin ratio and severity of coronary artery disease
Ola Hassan Abd Elaziz, Bassem Mohamed Abdel Hady, Ghada Mohamed S Ahmad, Safaa Abo Alfadl Mohamed, Abeer Ahmed Elmalah, Inass Hassan Ahmad, Entesar O Elsaghier, Marwa FM Elsayed, Hala Naguib Mohamed, Marwa Khairy Abd Elwahab, Ahmed Salah
Electronic Journal of General Medicine.2024; 21(1): em556. CrossRef - Association of serum homocysteine levels with intestinal flora and cognitive function in schizophrenia
Hehua Li, Hanqiu Li, Zhimin Zhu, Xiang Xiong, Yuanyuan Huang, Yangdong Feng, Zezhi Li, Kai Wu, Fengchun Wu
Journal of Psychiatric Research.2023; 159: 258. CrossRef - Association analysis of gut microbiota-metabolites-neuroendocrine changes in male rats acute exposure to simulated altitude of 5500 m
Jianan Wang, Shiying Liu, Yalei Xie, Chengli Xu
Scientific Reports.2023;[Epub] CrossRef
- Homocysteine, Nutrition, and Gut Microbiota: A Comprehensive Review of Current Evidence and Insights
- Molecular characterization of the Saccharomycopsis fibuligera ATF genes, encoding alcohol acetyltransferase for volatile acetate ester formation
- Hye Yun Moon , Hyeon Jin Kim , Ki Seung Kim , Su Jin Yoo , Dong Wook Lee , Hee Je Shin , Jeong Ah Seo , Hyun Ah Kang
- J. Microbiol. 2021;59(6):598-608. Published online May 29, 2021
- DOI: https://doi.org/10.1007/s12275-021-1159-8
- 336 View
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- 10 Web of Science
- 7 Crossref
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Abstract
PDF
- Aroma ester components produced by fermenting yeast cells via alcohol acetyltransferase (AATase)-catalyzed intracellular reactions are responsible for the fruity character of fermented alcoholic beverages, such as beer and wine. Acetate esters are reportedly produced at relatively high concentrations by non-Saccharomyces species. Here, we identified 12 ATF orthologues (SfATFs) encoding putative AATases, in the diploid genome of Saccharomycopsis fibuligera KJJ81, an isolate from wheat-based Nuruk in Korea. The identified SfATF proteins (SfAtfp) display low sequence identities with S. cerevisiae Atf1p (between 13.3 and 27.0%). All SfAtfp identified, except SfAtf(A)4p and SfAtf(B)4p, contained the activation domain (HXXXD) conserved in other Atf proteins. Culture supernatant analysis using headspace gas chromatography mass spectrometry confirmed that the recombinant S. cerevisiae strains expressing SfAtf(A)2p, SfAtf(B)2p, and SfAtf(B)6p produced high levels of isoamyl and phenethyl acetates. The volatile aroma profiles generated by the SfAtf proteins were distinctive from that of S. cerevisiae Atf1p, implying difference in the substrate preference. Cellular localization analysis using GFP fusion revealed the localization of SfAtf proteins proximal to the lipid particles, consistent with the presence of amphipathic helices at their N- and C-termini. This is the first report that systematically characterizes the S. fibuligera ATF genes encoding functional AATases responsible for acetate ester formation using higher alcohols as substrate, demonstrating their biotechnological potential for volatile ester production.
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Citations
Citations to this article as recorded by- Investigating the role of primary fungi in Huangjiu fermentation: Insights from flavor orientation and synthetic microbiomes
Qi Peng, Huihui Zhou, Huajun Zheng, Guangfa Xie
Food Microbiology.2025; 129: 104765. CrossRef - Genome-Wide Identification and Biochemical Characterization of Alcohol Acyltransferases for Aroma Generation in Wickerhamomyces subpelliculosus Isolates from Fermented Food
Su Jin Yoo, Hyeon Jin Kim, Hye Yun Moon, Min-Seung Jeon, Yong Uk Cho, Che Ok Jeon, Seong-Il Eyun, Hyun Ah Kang
Journal of Agricultural and Food Chemistry.2024; 72(50): 28194. CrossRef -
Characterization and phylogenetic analysis of the complete mitochondrial genome of
Saccharomycopsis fibuligera
(lindner) Klocker 1907 (saccharomycetales: saccharomycopsidaceae)
Yue Deng, Guangjiu Chen, Xuedong Bao, Jie He
Mitochondrial DNA Part B.2024; 9(6): 743. CrossRef - Optimization of High-Density Fermentation Conditions for Saccharomycopsis fibuligera Y1402 through Response Surface Analysis
Hongyang Yuan, Qi Sun, Lanshuang Wang, Zhilei Fu, Tianze Zhou, Jinghao Ma, Xiaoyan Liu, Guangsen Fan, Chao Teng
Foods.2024; 13(10): 1546. CrossRef - Genomic and functional features of yeast species in Korean traditional fermented alcoholic beverage and soybean products
Da Min Jeong, Hyeon Jin Kim, Min-Seung Jeon, Su Jin Yoo, Hye Yun Moon, Eun-joo Jeon, Che Ok Jeon, Seong-il Eyun, Hyun Ah Kang
FEMS Yeast Research.2023;[Epub] CrossRef - Beer fermentation performance and sugar uptake of Saccharomycopsis fibuligera–A novel option for low-alcohol beer
Yvonne Methner, Frederico Magalhães, Luis Raihofer, Martin Zarnkow, Fritz Jacob, Mathias Hutzler
Frontiers in Microbiology.2022;[Epub] CrossRef - Comparative analysis of aroma components and quality of Geotrichum candidum after space mutation breeding
Junjie Chen, Qianying Li, Jie Wang, Weizhe Chen, Qikai Zheng, Qingping Zhong, Xiang Fang, Zhenlin Liao
Frontiers in Microbiology.2022;[Epub] CrossRef
- Investigating the role of primary fungi in Huangjiu fermentation: Insights from flavor orientation and synthetic microbiomes
- Mst1/2-ALK promotes NLRP3 inflammasome activation and cell apoptosis during Listeria monocytogenes infection
- Aijiao Gao , Huixin Tang , Qian Zhang , Ruiqing Liu , Lin Wang , Yashan Liu , Zhi Qi , Yanna Shen
- J. Microbiol. 2021;59(7):681-692. Published online April 20, 2021
- DOI: https://doi.org/10.1007/s12275-021-0638-2
- 379 View
- 0 Download
- 12 Web of Science
- 9 Crossref
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Abstract
PDF
- Listeria monocytogenes (L. monocytogenes) is a Gram-positive intracellular foodborne pathogen that causes severe diseases, such as meningitis and sepsis. The NLR family pyrin domain-containing 3 (NLRP3) inflammasome has been reported to participate in host defense against pathogen infection. However, the exact molecular mechanisms underlying NLRP3 inflammasome activation remain to be fully elucidated. In the present study, the roles of mammalian Ste20- like kinases 1/2 (Mst1/2) and Anaplastic Lymphoma Kinase (ALK) in the activation of the NLRP3 inflammasome induced by L. monocytogenes infection were investigated. The expression levels of Mst1/2, phospho (p)-ALK, p-JNK, Nek7, and NLRP3 downstream molecules including activated caspase- 1 (p20) and mature interleukin (IL)-1β (p17), were upregulated in L. monocytogenes-infected macrophages. The ALK inhibitor significantly decreased the expression of p-JNK, Nek7, and NLRP3 downstream molecules in macrophages infected with L. monocytogenes. Furthermore, the Mst1/2 inhibitor markedly inhibited the L. monocytogenes-induced activation of ALK, subsequently downregulating the expression of p-JNK, Nek7, and NLRP3 downstream molecules. Therefore, our study demonstrated that Mst1/2-ALK mediated the activation of the NLRP3 inflammasome by promoting the interaction between Nek7 and NLRP3 via JNK during L. monocytogenes infection, which subsequently increased the maturation and release of proinflammatory cytokine to resist pathogen infection. Moreover, Listeriolysin O played a key role in the process. In addition, we also found that the L. monocytogenes-induced apoptosis of J774A.1 cells was reduced by the Mst1/2 or ALK inhibitor. The present study reported, for the first time, that the Mst1/2-ALK-JNK-NLRP3 signaling pathway plays a vital proinflammatory role during L. monocytogenes infection.
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Citations
Citations to this article as recorded by- PDE4B promotes JNK/NLRP3 activation in the nucleus pulposus and mediates intervertebral disc degeneration
Weixing Xu, Rana Dhar, Kaiyue Li, Danyang Zheng, Minxin He, Weiguo Ding, Long Xin, Bin Xu, Yuqing He, Qi Peng, Huifang Tang
Scientific Reports.2025;[Epub] CrossRef - IL-18 biology in severe asthma
Sarita Thawanaphong, Aswathi Nair, Emily Volfson, Parameswaran Nair, Manali Mukherjee
Frontiers in Medicine.2024;[Epub] CrossRef - TRAF6-TAK1-IKKβ pathway mediates TLR2 agonists activating “one-step” NLRP3 inflammasome in human monocytes
Mengdan Chen, Shi Yu, Yuhui Gao, Jiaxun Li, Xun Wang, Bin Wei, Guangxun Meng
Cytokine.2023; 169: 156302. CrossRef - ALK-JNK signaling promotes NLRP3 inflammasome activation and pyroptosis via NEK7 during Streptococcus pneumoniae infection
Xia Wang, Yan Zhao, Dan Wang, Chang Liu, Zhi Qi, Huixin Tang, Yashan Liu, Shiqi Zhang, Yali Cui, Yingying Li, Ruiqing Liu, Yanna Shen
Molecular Immunology.2023; 157: 78. CrossRef - Inflammasome activation by Gram-positive bacteria: Mechanisms of activation and regulation
A. Marijke Keestra-Gounder, Prescilla Emy Nagao
Frontiers in Immunology.2023;[Epub] CrossRef - Toxoplasma gondii profilin induces NLRP3 activation and IL-1β production/secretion in THP-1 cells
Hossein Pazoki, Hamed Mirjalali, Maryam Niyyati, Seyed Javad Seyed Tabaei, Nariman Mosaffa, Shabnam Shahrokh, Hamid Asadzadeh Ahdaei, Andreas Kupz, Mohammad Reza Zali
Microbial Pathogenesis.2023; 180: 106120. CrossRef - The Critical Role of Potassium Efflux and Nek7 in Pasteurella multocida-Induced NLRP3 Inflammasome Activation
Yu Wang, Zheng Zeng, Jinrong Ran, Lianci Peng, Xingping Wu, Chao Ye, Chunxia Dong, Yuanyi Peng, Rendong Fang
Frontiers in Microbiology.2022;[Epub] CrossRef - Coral and it's symbionts responses to the typical global marine pollutant BaP by 4D-Proteomics approach
Yuebin Pei, Shuai Chen, Yuting Zhang, Volovych Olga, Yuanchao Li, Xiaoping Diao, Hailong Zhou
Environmental Pollution.2022; 307: 119440. CrossRef - NEK7-Mediated Activation of NLRP3 Inflammasome Is Coordinated by Potassium Efflux/Syk/JNK Signaling During Staphylococcus aureus Infection
Ruiqing Liu, Yashan Liu, Chang Liu, Aijiao Gao, Lin Wang, Huixin Tang, Qiang Wu, Xia Wang, Derun Tian, Zhi Qi, Yanna Shen
Frontiers in Immunology.2021;[Epub] CrossRef
- PDE4B promotes JNK/NLRP3 activation in the nucleus pulposus and mediates intervertebral disc degeneration
Review
- [MINIREVIEW]Regulation of gene expression by protein lysine acetylation in Salmonella
- Hyojeong Koo , Shinae Park , Min-Kyu Kwak , Jung-Shin Lee
- J. Microbiol. 2020;58(12):979-987. Published online November 17, 2020
- DOI: https://doi.org/10.1007/s12275-020-0483-8
- 468 View
- 0 Download
- 17 Web of Science
- 15 Crossref
-
Abstract
PDF
- Protein lysine acetylation influences many physiological functions, such as gene regulation, metabolism, and disease in eukaryotes. Although little is known about the role of lysine acetylation in bacteria, several reports have proposed its importance in various cellular processes. Here, we discussed the function of the protein lysine acetylation and the post-translational modifications (PTMs) of histone-like proteins in bacteria focusing on Salmonella pathogenicity. The protein lysine residue in Salmonella is acetylated by the Pat-mediated enzymatic pathway or by the acetyl phosphate-mediated non-enzymatic pathway. In Salmonella, the acetylation of lysine 102 and lysine 201 on PhoP inhibits its protein activity and DNAbinding, respectively. Lysine acetylation of the transcriptional regulator, HilD, also inhibits pathogenic gene expression. Moreover, it has been reported that the protein acetylation patterns significantly differ in the drug-resistant and -sensitive Salmonella strains. In addition, nucleoid-associated proteins such as histone-like nucleoid structuring protein (H-NS) are critical for the gene silencing in bacteria, and PTMs in H-NS also affect the gene expression. In this review, we suggest that protein lysine acetylation and the post-translational modifications of H-NS are important factors in understanding the regulation of gene expression responsible for pathogenicity in Salmonella.
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Citations
Citations to this article as recorded by- Global Insights into the Lysine Acetylome Reveal the Role of Lysine Acetylation in the Adaptation of Bacillus altitudinis to Salt Stress
Xujian Li, Shanshan Dai, Shanshan Sun, Dongying Zhao, Hui Li, Junyi Zhang, Jie Ma, Binghai Du, Yanqin Ding
Journal of Proteome Research.2025; 24(1): 210. CrossRef - The concentration of medium-chain fatty acids in breast milk is influenced by maternal diet and associated with gut microbiota in infants
Menglu Xi, Ignatius Man-Yau Szeto, Sufang Duan, Ting Li, Yalu Yan, Xia Ma, Ting Sun, Weilian Hung, Celi Yang, Yonghua Zhang, Ai Zhao
Journal of Functional Foods.2025; 128: 106782. CrossRef - Reversible acetylation of ribosomal protein S1 serves as a smart switch for Salmonella to rapidly adapt to host stress
Yi-Lin Shen, Tian-Xian Liu, Lei Xu, Bang-Ce Ye, Ying Zhou
Nucleic Acids Research.2025;[Epub] CrossRef - Bacterial protein acetylation: mechanisms, functions, and methods for study
Jocelin Rizo, Sergio Encarnación-Guevara
Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef - Acetyl-proteome profiling revealed the role of lysine acetylation in erythromycin resistance of Staphylococcus aureus
Miao Feng, Xiaoyu Yi, Yanling Feng, Feng He, Zonghui Xiao, Hailan Yao
Heliyon.2024; 10(15): e35326. CrossRef - Short-chain fatty acids in breast milk and their relationship with the infant gut microbiota
Menglu Xi, Yalu Yan, Sufang Duan, Ting Li, Ignatius Man-Yau Szeto, Ai Zhao
Frontiers in Microbiology.2024;[Epub] CrossRef - Acetylomics reveals an extensive acetylation diversity within Pseudomonas aeruginosa
Nand Broeckaert, Hannelore Longin, Hanne Hendrix, Jeroen De Smet, Mirita Franz-Wachtel, Boris Maček, Vera van Noort, Rob Lavigne
microLife.2024;[Epub] CrossRef - Lysine acetylation regulates the AT-rich DNA possession ability of H-NS
Yabo Liu, Mengqing Zhou, Yifan Bu, Liang Qin, Yuanxing Zhang, Shuai Shao, Qiyao Wang
Nucleic Acids Research.2024; 52(4): 1645. CrossRef -
Acetylation of K188 and K192 inhibits the DNA-binding ability of NarL to regulate
Salmonella
virulence
Liu-Qing Zhang, Yi-Lin Shen, Bang-Ce Ye, Ying Zhou, Christopher A. Elkins
Applied and Environmental Microbiology.2023;[Epub] CrossRef - Acetylome and Succinylome Profiling of Edwardsiella tarda Reveals Key Roles of Both Lysine Acylations in Bacterial Antibiotic Resistance
Yuying Fu, Lishan Zhang, Huanhuan Song, Junyan Liao, Li Lin, Wenjia Jiang, Xiaoyun Wu, Guibin Wang
Antibiotics.2022; 11(7): 841. CrossRef - Pat- and Pta-mediated protein acetylation is required for horizontally-acquired virulence gene expression in Salmonella Typhimurium
Hyojeong Koo, Eunna Choi, Shinae Park, Eun-Jin Lee, Jung-Shin Lee
Journal of Microbiology.2022; 60(8): 823. CrossRef -
Acetylation of CspC Controls the Las Quorum-Sensing System through Translational Regulation of
rsaL
in Pseudomonas aeruginosa
Shouyi Li, Xuetao Gong, Liwen Yin, Xiaolei Pan, Yongxin Jin, Fang Bai, Zhihui Cheng, Un-Hwan Ha, Weihui Wu, Pierre Cornelis, Gerald B. Pier
mBio.2022;[Epub] CrossRef - Trans-acting regulators of ribonuclease activity
Jaejin Lee, Minho Lee, Kangseok Lee
Journal of Microbiology.2021; 59(4): 341. CrossRef - Acetylation of the CspA family protein CspC controls the type III secretion system through translational regulation ofexsAinPseudomonas aeruginosa
Shouyi Li, Yuding Weng, Xiaoxiao Li, Zhuo Yue, Zhouyi Chai, Xinxin Zhang, Xuetao Gong, Xiaolei Pan, Yongxin Jin, Fang Bai, Zhihui Cheng, Weihui Wu
Nucleic Acids Research.2021; 49(12): 6756. CrossRef - Transcriptional Regulation of the Multiple Resistance Mechanisms in Salmonella—A Review
Michał Wójcicki, Olga Świder, Kamila J. Daniluk, Paulina Średnicka, Monika Akimowicz, Marek Ł. Roszko, Barbara Sokołowska, Edyta Juszczuk-Kubiak
Pathogens.2021; 10(7): 801. CrossRef
- Global Insights into the Lysine Acetylome Reveal the Role of Lysine Acetylation in the Adaptation of Bacillus altitudinis to Salt Stress
Journal Articles
- Omp16, a conserved peptidoglycan-associated lipoprotein, is involved in Brucella virulence in vitro
- Feijie Zhi , Dong Zhou , Junmei Li , Lulu Tian , Guangdong Zhang , Yaping Jin , Aihua Wang
- J. Microbiol. 2020;58(9):793-804. Published online September 1, 2020
- DOI: https://doi.org/10.1007/s12275-020-0144-y
- 388 View
- 0 Download
- 15 Web of Science
- 15 Crossref
-
Abstract
PDF
- Brucella, the bacterial agent of common zoonotic brucellosis, primarily infects specific animal species. The Brucella outer membrane proteins (Omps) are particularly attractive for developing vaccine and improving diagnostic tests and are associated with the virulence of smooth Brucella strains. Omp16 is a homologue to peptidoglycan-associated lipoproteins (Pals), and an omp16 mutant has not been generated in any Brucella strain until now. Very little is known about the functions and pathogenic mechanisms of Omp16 in Brucella. Here, we confirmed that Omp16 has a conserved Pal domain and is highly conserved in Brucella. We attempted to delete omp16 in Brucella suis vaccine strain 2 (B. suis S2) without success, which shows that Omp16 is vital for Brucella survival. We acquired a B. suis S2 Omp16 mutant via conditional complementation. Omp16 deficiency impaired Brucella outer membrane integrity and activity in vitro. Moreover, inactivation of Omp16 decreased bacterial intracellular survival in macrophage RAW 264.7 cells. B. suis S2 and its derivatives induced marked expression of IL-1β, IL-6, and TNF-α mRNA in Raw 264.7 cells. Whereas inactivation of Omp16 in Brucella enhanced IL-1β and IL-6 expression in Raw 264.7 cells. Altogether, these findings show that the Brucella Omp16 mutant was obtained via conditional complementation and confirmed that Omp16 can maintain outer membrane integrity and be involved in bacterial virulence in Brucella in vitro and in vivo. These results will be important in uncovering the pathogenic mechanisms of Brucella.
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Citations
Citations to this article as recorded by-
Neurobrucellosis (
Brucella ceti
) in striped dolphins (
Stenella coeruleoalba
): Immunohistochemical studies on immune response and neuroinflammation
Agustín Rebollada-Merino, Federica Giorda, Martí Pumarola, Laura Martino, Alberto Gomez-Buendia, Umberto Romani-Cremaschi, Cristina Casalone, Virginia Mattioda, Fabio Di Nocera, Giuseppe Lucifora, Antonio Petrella, Lucas Domínguez, Mariano Domingo, Carla
Veterinary Pathology.2025; 62(2): 226. CrossRef - Enhancing host defense against Brucella: The immune effect exerted by anti-OMP16 monoclonal antibody
Yunyi Zhai, Hui Wang, Kaihui Sun, Ye Yuan, Shurong Yin, Jiaoyang Fang, Weifang Zheng, Gaowa Wudong, Xiaofang Liu, Yuanhao Yang, Dong Zhou, Wei Liu, Yaping Jin, Aihua Wang
International Immunopharmacology.2025; 148: 114142. CrossRef - Brucellosis: Bacteriology, pathogenesis, epidemiology and role of the metallophores in virulence: a review
Ghassan Ghssein, Zeinab Ezzeddine, Sima Tokajian, Charbel Al Khoury, Hussein Kobeissy, Jose-Noel Ibrahim, Christelle Iskandar, Hussein F. Hassan
Frontiers in Cellular and Infection Microbiology.2025;[Epub] CrossRef - The Role of Outer Membrane Protein 16 in Brucella Pathogenesis, Vaccine Development, and Diagnostic Applications
Lu Zhang, Jun Bai, Long Li, Yanqing Jia, Xinxin Qiu, Yan Luo, Dong Zhou, Zhencang Zhang
Veterinary Sciences.2025; 12(7): 605. CrossRef -
Pathogenicity and virulence of
Brucella
: Strategies for metabolic adaptation and immune evasion
Erika S. Guimarães, Marco Tulio R. Gomes, Ana Carolina V. S. C. de Araujo, Karla Karoline S. Ramos, Sergio C. Oliveira
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Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef - A Thermosensitive and Degradable Chitin-Based Hydrogel as a Brucellosis Vaccine Adjuvant
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Neurobrucellosis (
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- Evaluation and application of constitutive promoters for cutinase production by Saccharomyces cerevisiae
- Juan Zhang , Yanqiu Cai , Guocheng Du , Jian Chen , Miao Wang , Zhen Kang
- J. Microbiol. 2017;55(7):538-544. Published online June 30, 2017
- DOI: https://doi.org/10.1007/s12275-017-6514-4
- 326 View
- 0 Download
- 5 Crossref
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Abstract
PDF
- died and applied in processes targeted for industrial scale. In this work, the cutinase gene tfu from Thermobifida fusca was artificially synthesized according to codon usage bias of Saccharomyces cerevisiae and investigated in Saccharomyces cerevisiae. Using the α-factor signal peptide, the T. fusca cutinase was successfully overexpressed and secreted with the GAL1 expression system. To increase the cutinase level and overcome some of the drawbacks of induction, four different strong promoters (ADH1, HXT1, TEF1, and TDH3) were comparatively evaluated for cutinase production. By comparison, promoter TEF1 exhibited an outstanding property and significantly increased the expression level. By fed-batch fermentation with a constant feeding approach, the activity of cutinase was increased to 29.7 U/ml. The result will contribute to apply constitutive promoter TEF1 as a tool for targeted cutinase production in S. cerevisiae cell factory.
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- Woo-Hyun Chung ,
- J. Microbiol. 2017;55(6):409-416. Published online March 9, 2017
- DOI: https://doi.org/10.1007/s12275-017-6647-5
- 387 View
- 0 Download
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Abstract
PDF
- To deal with chemically reactive oxygen molecules constantly threatening aerobic life, cells are readily equipped with elabo-rate biological antioxidant systems. Superoxide dismutase is a metalloenzyme catalytically eliminating superoxide radi-cal as a first-line defense mechanism against oxidative stress. Multiple different SOD isoforms have been developed through-out evolution to play distinct roles in separate subcellular com-partments. SOD is not essential for viability of most aerobic organisms and intriguingly found even in strictly anaerobic bacteria. Sod1 has recently been known to play important roles as a nuclear transcription factor, an RNA binding pro-tein, a synthetic lethal interactor, and a signal modulator in glucose metabolism, most of which are independent of its canonical function as an antioxidant enzyme. In this review, recent advances in understanding the unconventional role of Sod1 are highlighted and discussed with an emphasis on its genetic crosstalk with DNA damage repair/checkpoint path-ways. The budding yeast Saccharomyces cerevisiae has been successfully used as an efficient tool and a model organism to investigate a number of novel functions of Sod1.
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Research Support, Non-U.S. Gov'ts
- Identification of Psk2, Skp1, and Tub4 as trans-acting factors for uORF-containing ROK1 mRNA in Saccharomyces cerevisiae
- Soonmee Jeon , Suran Lim , Jeemin Ha , Jinmi Kim
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- DOI: https://doi.org/10.1007/s12275-015-5389-5
- 326 View
- 0 Download
- 2 Crossref
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Abstract
PDF
- Rok1, a DEAD-box RNA helicase, is involved in rRNA processing and the control of cell cycle progression in Saccharomyces cerevisiae. Rok1 protein expression is cell cycle-regulated, declining at G1/S and increasing at G2. The downregulation of Rok1 expression in G1/S phase is mediated by the inhibitory action of two upstream open reading frames (uORFs) in the ROK1 5-untranslated region (5UTR). We identified Psk2 (PAS kinase), Skp1 (kinetochore protein) and Tub4 (γ-tubulin protein) as ROK1 5UTR-interacting proteins using yeast three-hybrid system. A deletion analysis of PSK2 or inactivation of temperature-sensitive alleles of SKP1 and TUB4 revealed that Rok1 protein synthesis is repressed by Psk2 and Skp1. This repression appeared to be mediated through the ROK1 uORF1. In contrast, Tub4 plays a positive role in regulating Rok1 protein synthesis and likely after the uORF1-mediated inhibitory regulation. These results suggest that 5UTR-interacting proteins, identified using three hybrid screening, are important for uORF-mediated regulation of Rok1 protein expression.
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Citations
Citations to this article as recorded by- Identification of short open reading frames in plant genomes
Yong Feng, Mengyun Jiang, Weichang Yu, Jiannan Zhou
Frontiers in Plant Science.2023;[Epub] CrossRef - HST1 increases replicative lifespan of a sir2Δ mutant in the absence of PDE2 in Saccharomyces cerevisiae
Woo Kyu Kang, Mayur Devare, Jeong-Yoon Kim
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- Growth Phase-dependent Roles of Sir2 in Oxidative Stress Resistance and Chronological Lifespan in Yeast
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- J. Microbiol. 2014;52(8):652-658. Published online July 5, 2014
- DOI: https://doi.org/10.1007/s12275-014-4173-2
- 381 View
- 2 Download
- 10 Crossref
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Abstract
PDF
- Silent Information Regulator 2 (Sir2), a conserved NAD+- dependent histone deacetylase, has been implicated as one of the key factors in regulating stress response and longevity. Here, we report that the role of Sir2 in oxidative stress resistance and chronological lifespan is dependent on growth phase in yeast. In exponential phase, sir2Δ cells were more resistant to H2O2 stress and had a longer chronological lifespan than wild type. By contrast, in post-diauxic phase, sir2Δ cells were less resistant to H2O2 stress and had a shorter chronological lifespan than wild type cells. Similarly, the expression of antioxidant genes, which are essential to cope with oxidative stress, was regulated by Sir2 in a growth phasedependent manner. Collectively, our findings highlight the importance of the metabolic state of the cell in determining whether Sir2 can protect against or accelerate cellular aging of yeast.
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Review
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- Woo-Hyun Chung
- J. Microbiol. 2014;52(2):89-98. Published online February 1, 2014
- DOI: https://doi.org/10.1007/s12275-014-3524-3
- 356 View
- 0 Download
- 23 Crossref
-
Abstract
PDF
- Pif1 DNA helicase is the prototypical member of a 5' to 3' helicase superfamily conserved from bacteria to humans. In Saccharomyces cerevisiae, Pif1 and its homologue Rrm3, localize in both mitochondria and nucleus playing multiple roles in the maintenance of genomic homeostasis. They display relatively weak processivities in vitro, but have largely non-overlapping functions on common genomic loci such as mitochondrial DNA, telomeric ends, and many replication forks especially at hard-to-replicate regions including ribosomal DNA and G-quadruplex structures. Recently, emerging evidence shows that Pif1, but not Rrm3, has a significant new role in repair-associated DNA synthesis with Polδ during homologous recombination stimulating D-loop migration for conservative DNA replication. Comparative genetic and biochemical studies on the structure and function of Pif1 family helicases across different biological systems are further needed to elucidate both diversity and specificity of their mechanisms of action that contribute to genome stability.
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Nannan Su, Alicia K. Byrd, Sakshibeedu R. Bharath, Olivia Yang, Yu Jia, Xuhua Tang, Taekjip Ha, Kevin D. Raney, Haiwei Song
Nature Communications.2019;[Epub] CrossRef - The nature of meiotic chromosome dynamics and recombination in budding yeast
Soogil Hong, Jeong Hwan Joo, Hyeseon Yun, Keunpil Kim
Journal of Microbiology.2019; 57(4): 221. CrossRef - The Drosophila melanogaster PIF1 Helicase Promotes Survival During Replication Stress and Processive DNA Synthesis During Double-Strand Gap Repair
Ece Kocak, Sarah Dykstra, Alexandra Nemeth, Catherine G Coughlin, Kasey Rodgers, Mitch McVey
Genetics.2019; 213(3): 835. CrossRef - The signature motif of the Saccharomyces cerevisiae Pif1 DNA helicase is essential in vivo for mitochondrial and nuclear functions and in vitro for ATPase activity
Carly L Geronimo, Saurabh P Singh, Roberto Galletto, Virginia A Zakian
Nucleic Acids Research.2018; 46(16): 8357. CrossRef - DNA-unwinding activity of Saccharomyces cerevisiae Pif1 is modulated by thermal stability, folding conformation, and loop lengths of G-quadruplex DNA
Lei Wang, Qing-Man Wang, Yi-Ran Wang, Xu-Guang Xi, Xi-Miao Hou
Journal of Biological Chemistry.2018; 293(48): 18504. CrossRef - Role of the Pif1-PCNA Complex in Pol δ-Dependent Strand Displacement DNA Synthesis and Break-Induced Replication
Olga Buzovetsky, Youngho Kwon, Nhung Tuyet Pham, Claire Kim, Grzegorz Ira, Patrick Sung, Yong Xiong
Cell Reports.2017; 21(7): 1707. CrossRef - Structure and function of Pif1 helicase
Alicia K. Byrd, Kevin D. Raney
Biochemical Society Transactions.2017; 45(5): 1159. CrossRef - Mechanistic and biological considerations of oxidatively damaged DNA for helicase-dependent pathways of nucleic acid metabolism
Jack D. Crouch, Robert M. Brosh
Free Radical Biology and Medicine.2017; 107: 245. CrossRef - PIF1 family DNA helicases suppress R-loop mediated genome instability at tRNA genes
Phong Lan Thao Tran, Thomas J. Pohl, Chi-Fu Chen, Angela Chan, Sebastian Pott, Virginia A. Zakian
Nature Communications.2017;[Epub] CrossRef - Yeast Helicase Pif1 Unwinds RNA:DNA Hybrids with Higher Processivity than DNA:DNA Duplexes
Shubeena Chib, Alicia K. Byrd, Kevin D. Raney
Journal of Biological Chemistry.2016; 291(11): 5889. CrossRef - Getting it done at the ends: Pif1 family DNA helicases and telomeres
Carly L. Geronimo, Virginia A. Zakian
DNA Repair.2016; 44: 151. CrossRef - Genetic instability in budding and fission yeast—sources and mechanisms
Adrianna Skoneczna, Aneta Kaniak, Marek Skoneczny, Antoine Danchin
FEMS Microbiology Reviews.2015; 39(6): 917. CrossRef - TheBacteroides sp. 3_1_23Pif1 protein is a multifunctional helicase
Na-Nv Liu, Xiao-Lei Duan, Xia Ai, Yan-Tao Yang, Ming Li, Shuo-Xing Dou, Stephane Rety, Eric Deprez, Xu-Guang Xi
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Ramanagouda Ramanagoudr-Bhojappa, Alicia K. Byrd, Christopher Dahl, Kevin D. Raney
Biochemistry.2014; 53(48): 7659. CrossRef
- Signification and Application of Mutator and Antimutator Phenotype-Induced Genetic Variations in Evolutionary Adaptation and Cancer Therapeutics
Research Support, Non-U.S. Gov'ts
- Isolation and Functional Characterization of a Delta 6-Desaturase Gene from the Pike Eel (Muraenesox cinereus)
- Sun Hee Kim , Kyung Hee Roh , Jung-Bong Kim , Kwang-Soo Kim , Nam Shin Kim , Hyun Uk Kim , Kyeong-Ryeol Lee , Jong-Sug Park , Jong-Bum Kim
- J. Microbiol. 2013;51(6):807-813. Published online October 5, 2013
- DOI: https://doi.org/10.1007/s12275-013-3144-3
- 351 View
- 0 Download
- 9 Crossref
-
Abstract
PDF
-
Stearidonic acid (STA; 18:4n-3) and γ-linolenic acid (GLA;
18:3n-6) are significant intermediates in the biosynthetic pathway
for the very-long-chain polyunsaturated fatty acids of
eicosapentaenoic acid (EPA; 20:5n-3) and arachidonic acid
(ARA; 20:4n-6), respectively. To develop a sustainable system
for the production of dietary polyunsaturated fatty acids,
we focused on the action of the enzyme delta 6-desaturase
(D6DES) on the essential acids, linoleic acid (LA; 18:2n-6)
and α-linolenic acid (ALA; 18:3n-3). A 1,335-bp full-length
cDNA encoding D6DES (McD6DES) was cloned from Muraenesox
cinereus using degenerate PCR and RACE-PCR
methods
. To investigate the enzymatic activity of McD6DES in the production of n-6 and n-3 fatty acids, a recombinant plasmid expressing McD6DES (pYES-McD6DES) was transformed into and expressed in Saccharomyces cerevisiae. The exogenously expressed McD6DES produced GLA and STA at conversion rates of 14.2% and 45.9%, respectively, from the exogenous LA and ALA substrates. These results indicate that McD6DES is essentially a delta 6-desaturase involved in very-long-chain polyunsaturated fatty acid synthesis. -
Citations
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Dizhi Xie, Cuiying Chen, Yewei Dong, Cuihong You, Shuqi Wang, Óscar Monroig, Douglas R. Tocher, Yuanyou Li
Progress in Lipid Research.2021; 82: 101095. CrossRef - Comparative and functional analysis of desaturase FADS1 (∆5) and FADS2 (∆6) orthologues of marine organisms
Crisalejandra Rivera-Pérez, Fausto Valenzuela-Quiñonez, Javier Caraveo-Patiño
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics.2020; 35: 100704. CrossRef - Δ6 fatty acid desaturases in polyunsaturated fatty acid biosynthesis: insights into the evolution, function with substrate specificities and biotechnological use
Jie Cui, Haiqin Chen, Xin Tang, Jianxin Zhao, Hao Zhang, Yong Q. Chen, Wei Chen
Applied Microbiology and Biotechnology.2020; 104(23): 9947. CrossRef - In Silico Structural Studies and Molecular Docking Analysis of Delta6-desaturase in HUFA Biosynthetic Pathway
Suvra Roy, Hirak jyoti Chakraborty, Vikash Kumar, B K Behera, R S Rana, Gireesh Babu
Animal Biotechnology.2018; 29(3): 161. CrossRef - Changes in Plasma and Tissue Long-Chain Polyunsaturated Fatty Acid (LC-PUFA) Content in the Eel Anguilla japonica After External and Internal Osmotic Stress
Qinghao Zhang, Marty K. S. Wong, Yiqi Li, Yuanyou Li, Yoshio Takei
Zoological Science.2017; 34(5): 429. CrossRef - Effect of low temperature on highly unsaturated fatty acid biosynthesis in activated sludge
Su He, Li-li Ding, Ke Xu, Jin-ju Geng, Hong-qiang Ren
Bioresource Technology.2016; 211: 494. CrossRef - Metabolic engineering to produce γ-linolenic acid in Brassica napus using a Δ6-desaturase from pike eel
Sun Hee Kim, Kyung Hee Roh, Kyeong-Ryeol Lee, Han-Chul Kang, Hyun Uk Kim, Jong Bum Kim
Plant Biotechnology Reports.2016; 10(6): 475. CrossRef - Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids inArabidopsis
Sun Hee Kim, Kyung Hee Roh, Jong-Sug Park, Kwang-Soo Kim, Hyun Uk Kim, Kyeong-Ryeol Lee, Han-Chul Kang, Jong-Bum Kim
BioMed Research International.2015; 2015: 1. CrossRef - Coexpression of multiple genes reconstitutes two pathways of very long-chain polyunsaturated fatty acid biosynthesis in Pichia pastoris
Sun Hee Kim, Kyung Hee Roh, Kwang-Soo Kim, Hyun Uk Kim, Kyeong-Ryeol Lee, Han-Chul Kang, Jong-Bum Kim
Biotechnology Letters.2014; 36(9): 1843. CrossRef
- Regulation of long-chain polyunsaturated fatty acid biosynthesis in teleost fish
- Saccharomyces cerevisiae Can Secrete Sapp1p Proteinase of Candida parapsilosis But Cannot Use It for Efficient Nitrogen Acquisition
- Zuzana Vinterová , Václava Bauerová , Ji&# , Hana Sychrová , Olga Hru&# , Iva Pichová
- J. Microbiol. 2013;51(3):336-344. Published online June 28, 2013
- DOI: https://doi.org/10.1007/s12275-013-2422-4
- 281 View
- 0 Download
- 2 Crossref
-
Abstract
PDF
- Secreted aspartic proteinase Sapp1p of Candida parapsilosis represents one of the factors contributing to the pathogenicity of the fungus. The proteinase is synthesized as an inactive pre-pro-enzyme, but only processed Sapp1p is secreted into extracellular space. We constructed a plasmid containing the SAPP1 coding sequence under control of the ScGAL1 promoter and used it for proteinase expression in a Saccharomyces cerevisiae kex2Δ mutant. Because Sapp1p maturation depends on cleavage by Kex2p proteinase, the kex2Δ mutant secreted only the pro-form of Sapp1p. Characterization of this secreted proteinase form revealed that the Sapp1p signal peptide consists of 23 amino acids. Additionally, we prepared a plasmid with the SAPP1 coding sequence under control of its authentic CpSAPP1 promoter, which contains two GATAA motifs. While in C. parapsilosis SAPP1 expression is repressed by good low molecular weight nitrogen sources (e.g., ammonium ions), S. cerevisiae cells harboring this plasmid secreted a low concentration of active proteinase regardless of the type of nitrogen source used. Quantitative real-time PCR analysis of a set of genes related to nitrogen metabolism and uptake (GAT1, GLN3, STP2, GAP1, OPT1, and PTR2) obtained from S. cerevisiae cells transformed with either plasmid encoding SAPP1 under control of its own promoter or empty vector and cultivated in media containing various nitrogen sources also suggested that SAPP1 expression can be connected with the S. cerevisiae regulatory network. However, this regulation occurs in a different manner than in C. parapsilosis.
-
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Xiufang Liu, Mulin Lian, Mouming Zhao, Mingtao Huang
World Journal of Microbiology and Biotechnology.2024;[Epub] CrossRef - Pathogenic Candida species differ in the ability to grow at limiting potassium concentrations
B. Hušeková, H. Elicharová, H. Sychrová
Canadian Journal of Microbiology.2016; 62(5): 394. CrossRef
- Advances in recombinant protease production: current state and perspectives
- NOTE] Identification of Chaperones in Freeze Tolerance in Saccharomyces cerevisiae
- Mahendran Chinnamara Naicker , I Seul Jo , Hana Im
- J. Microbiol. 2012;50(5):882-887. Published online November 4, 2012
- DOI: https://doi.org/10.1007/s12275-012-2411-z
- 194 View
- 0 Download
- 10 Scopus
-
Abstract
- Exposure to low temperatures reduces protein folding rates and induces the cold denaturation of proteins. Considering the roles played by chaperones in facilitating protein folding and preventing protein aggregation, chaperones must exist that confer tolerance to cold stress. Here, yeast strains lacking individual chaperones were screened for reduced freezing tolerance. In total, 19 of 82 chaperone-deleted strains tested were more sensitive to freeze-thaw treatment than wild-type cells. The reintroduction of the respective chaperone genes into the deletion mutants recovered the freeze tolerance. The freeze sensitivity of the chaperone-knockout strains was also retained in the presence of 20% glycerol.
- The Production and Immunogenicity of Human Papillomavirus Type 58 Virus-like Particles Produced in Saccharomyces cerevisiae
- Hye-Lim Kwag , Hyoung Jin Kim , Don Yong Chang , Hong-Jin Kim
- J. Microbiol. 2012;50(5):813-820. Published online November 4, 2012
- DOI: https://doi.org/10.1007/s12275-012-2292-1
- 301 View
- 0 Download
- 14 Crossref
-
Abstract
- Human papillomavirus (HPV) is the cause of most cases of cervical cancer. HPV type 58 (HPV58) is the second most frequent cause of cervical cancer and high-grade squamous intraepithelial lesions (HSIL) in Asia and South / Central America, respectively. However, there is no vaccine against HPV58, although there are commercially available vaccines against HPV16 and 18. In this study, we produced HPV58 L1 protein from Saccharomyces cerevisiae, and investigated its immunogenicity. We first determined the optimum period of culture for obtaining HPV58 L1. We found that a considerable portion of the HPV58 L1 resulting from 48 h culture cannot be recovered by purification, while the HPV58 L1 resulting from 144 h culture is recovered efficiently: the yield of HPV58 L1 finally recovered from 144 h culture was 2.3 times higher than that from 48 h culture, although the production level of L1 protein from 144 h culture was lower than that from 48 h culture. These results indicate that the proportion of functional L1 protein from 144 h-cultured cells is significantly higher than that of 48 h-cultured cells. The HPV58 L1 purified from the 144 h culture was correctly assembled into structures similar to naturally occurring HPV virions. Immunization with the HPV58 L1 efficiently elicited anti-HPV58 neutralizing antibodies and antigen-specific CD4+ and CD8+ T cell proliferations, without the need for adjuvant. Our findings provide a convenient method for obtaining substantial amounts of highly immunogenic HPV58 L1 from S. cerevisiae.
-
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Citations to this article as recorded by- Expression, purification, and immunogenicity study of human papillomavirus type 52 virus-like particles produced in Hansenula polymorpha
Sheila Chairunnisa, Apon Zaenal Mustopa, Budiman Bela, Rosyida Khusniatul Arifah, Rifqiyah Nur Umami, Moh Egy Rahman Firdaus, Nurlaili Ekawati, Herman Irawan, Shasmita Irawan, Maritsa Nurfatwa, Ai Hertati, Sri Swasthikawati, Ela Novianti, Arizah Kusumawat
Biologicals.2025; 90: 101831. CrossRef - Yeast and Virus-like Particles: A Perfect or Imperfect Couple?
Sara Brachelente, Alvaro Galli, Tiziana Cervelli
Applied Microbiology.2023; 3(3): 805. CrossRef - Yeast-Based Virus-like Particles as an Emerging Platform for Vaccine Development and Delivery
Vartika Srivastava, Kripa N. Nand, Aijaz Ahmad, Ravinder Kumar
Vaccines.2023; 11(2): 479. CrossRef - How far have we explored fungi to fight cancer?
Chee Wun How, Yong Sze Ong, Sze Shin Low, Ashok Pandey, Pau Loke Show, Jhi Biau Foo
Seminars in Cancer Biology.2022; 86: 976. CrossRef - Yeast-based vaccines: New perspective in vaccine development and application
Ravinder Kumar, Piyush Kumar
FEMS Yeast Research.2019;[Epub] CrossRef - Advances in Designing and Developing Vaccines, Drugs and Therapeutic Approaches to Counter Human Papilloma Virus
Maryam Dadar, Sandip Chakraborty, Kuldeep Dhama, Minakshi Prasad, Rekha Khandia, Sameer Hassan, Ashok Munjal, Ruchi Tiwari, Kumaragurubaran Karthik, Deepak Kumar, Hafiz M. N. Iqbal, Wanpen Chaicumpa
Frontiers in Immunology.2018;[Epub] CrossRef - The codon-optimized capsid gene of duck circovirus can be highly expressed in yeast and self-assemble into virus-like particles
Cui YANG, Yu XU, Ren-yong JIA, Si-yang LIU, Ming-shu WANG, De-kang ZHU, Shun CHEN, Ma-feng LIU, Xin-xin ZHAO, Kun-feng SUN, Bo JING, Zhong-qiong YIN, An-chun CHENG
Journal of Integrative Agriculture.2017; 16(7): 1601. CrossRef - A novel trivalent HPV 16/18/58 vaccine with anti-HPV 16 and 18 neutralizing antibody responses comparable to those induced by the Gardasil quadrivalent vaccine in rhesus macaque model
Fei Yin, Yajun Wang, Na Chen, Dunquan Jiang, Yefeng Qiu, Yan Wang, Mei Yan, Jianping Chen, Haijiang Zhang, Yongjiang Liu
Papillomavirus Research.2017; 3: 85. CrossRef - EV71 virus-like particles produced by co-expression of capsid proteins in yeast cells elicit humoral protective response against EV71 lethal challenge
Xiaowen Wang, Xiangqian Xiao, Miao Zhao, Wei Liu, Lin Pang, Xin Sun, Shan Cen, Burton B. Yang, Yuming Huang, Wang Sheng, Yi Zeng
BMC Research Notes.2016;[Epub] CrossRef - Therapeutic potential of an AcHERV-HPV L1 DNA vaccine
Hee-Jung Lee, Jong Kwang Yoon, Yoonki Heo, Hansam Cho, Yeondong Cho, Yongdae Gwon, Kang Chang Kim, Jiwon Choi, Jae Sung Lee, Yu-Kyoung Oh, Young Bong Kim
Journal of Microbiology.2015; 53(6): 415. CrossRef - Immunogenicity of a Trivalent Human Papillomavirus L1 DNA-Encapsidated, Non-Replicable Baculovirus Nanovaccine
Hansam Cho, Hee-Jung Lee, Yoon-Ki Heo, Yeondong Cho, Yong-Dae Gwon, Mi-Gyeong Kim, Ki Hoon Park, Yu-Kyoung Oh, Young Bong Kim, Shibo Jiang
PLoS ONE.2014; 9(4): e95961. CrossRef - The Concentration of Carbon Source in the Medium Affects the Quality of Virus-Like Particles of Human Papillomavirus Type 16 Produced in Saccharomyces cerevisiae
Hyoung Jin Kim, Yingji Jin, Hong-Jin Kim, Paulo Lee Ho
PLoS ONE.2014; 9(4): e94467. CrossRef - Panorámica de los receptores celulares del virus del papiloma humano y su repercusión en la purificación de partículas semejantes a virus
J.F. Beltrán-Lissabet
Vacunas.2014; 15(1-2): 29. CrossRef - Virus-like particles for enterovirus 71 produced from Saccharomyces cerevisiae potently elicits protective immune responses in mice
Hao-Yang Li, Jian-Feng Han, Cheng-Feng Qin, Rong Chen
Vaccine.2013; 31(32): 3281. CrossRef
- Expression, purification, and immunogenicity study of human papillomavirus type 52 virus-like particles produced in Hansenula polymorpha
- Thionine Increases Electricity Generation from Microbial Fuel Cell Using Saccharomyces cerevisiae and Exoelectrogenic Mixed Culture
- Mostafa Rahimnejad , Ghasem Darzi Najafpour , Ali Asghar Ghoreyshi , Farid Talebnia , Giuliano C. Premier , Gholamreza Bakeri , Jung Rae Kim , Sang-Eun Oh
- J. Microbiol. 2012;50(4):575-580. Published online August 25, 2012
- DOI: https://doi.org/10.1007/s12275-012-2135-0
- 204 View
- 0 Download
- 90 Scopus
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Abstract
PDF
- Microbial fuel cells (MFCs) have been shown to be capable of clean energy production through the oxidation of biodegradable organic waste using various bacterial species as biocatalysts. In this study we found Saccharomyces cerevisiae, previously known electrochemcially inactive or less active species, can be acclimated with an electron mediator thionine for electrogenic biofilm formation in MFC, and electricity production is improved with facilitation of electron transfer. Power generation of MFC was also significantly increased by thionine with both aerated and non-aerated cathode. With electrochemically active biofilm enriched with swine wastewater, MFC power increased more significantly by addition of thionine. The optimum mediator concentration was 500 mM of thionine with S. cerevisae in MFC with the maximum voltage and current generation in the microbial fuel cell were 420 mV and 700 mA/m2, respectively. Cyclic voltametry shows that thionine improves oxidizing and reducing capability in both pure culture and acclimated biofilm as compared to non-mediated cell. The results obtained indicated that thionine has great potential to enhance power generation from unmediated yeast or electrochemically active biofilm in MFC.
- NOTE] Ectopic Expression of Sweet Potato MuS1 Increases Acquired Stress Tolerance and Fermentation Yield in Saccharomyces cerevisiae
- Il-Sup Kim , Sun-Young Shin , Sun-Hyung Kim , Ho-Sung Yoon
- J. Microbiol. 2012;50(3):544-546. Published online June 30, 2012
- DOI: https://doi.org/10.1007/s12275-012-2043-3
- 224 View
- 0 Download
- 3 Crossref
-
Abstract
PDF
- The MuS1 gene is highly homologous to many stress-related proteins in plants. Here, we characterized whether a new candidate gene, MuS1, is related to multiple stress tolerance in yeast as it is in plants. Transgenic yeast strain expressing MuS1 were more resistant to hydrogen peroxide, menadione, high salinity, metals (i.e., cadmium, copper, iron, and zinc), ethanol, and lactic acid than wild-type strain transformed with a vector alone. In addition, the alcohol yield of the transgenic yeast strain was higher than that of the wild-type strain during the batch fermentation process. These results show that MuS1-expressing transgenic yeast strain exhibits enhanced alcohol yield as well as tolerance to abiotic stresses, especially metal stress.
-
Citations
Citations to this article as recorded by- The unique importance of sweetpotato: Insights focusing on genetic improvements of salt and drought tolerance
Mingku Zhu
Scientia Horticulturae.2025; 339: 113848. CrossRef - Recent advances in miRNA and siRNA approaches, and genome editing to augment biotic and abiotic stress tolerance in sweet potato (Ipomoea batatas L.)
Bin Song, Ali Raza, Fei He, Shuting Wang, Xuelian Huang, Aihui Mo, Kaifang Jiang, Jucheng Guo, Atul Kumar Srivastava, Aamir Riaz, Muhammad Ahmad Hassan, Zhangxun Wang
International Journal of Biological Macromolecules.2025; 327: 147195. CrossRef - The interaction networks of small rubber particle proteins in the latex of Taraxacum koksaghyz reveal diverse functions in stress responses and secondary metabolism
Silva Melissa Wolters, Natalie Laibach, Jenny Riekötter, Kai-Uwe Roelfs, Boje Müller, Jürgen Eirich, Richard M. Twyman, Iris Finkemeier, Dirk Prüfer, Christian Schulze Gronover
Frontiers in Plant Science.2024;[Epub] CrossRef
- The unique importance of sweetpotato: Insights focusing on genetic improvements of salt and drought tolerance
- Effects of Mutations in the WD40 Domain of α-COP on Its Interaction with the COPI Coatomer in Saccharomyces cerevisiae
- Ki-Hyun Kim , Eun Kyung Kim , Ki Young Jeong , Yun-Hee Park , Hee-Moon Park
- J. Microbiol. 2012;50(2):256-262. Published online April 27, 2012
- DOI: https://doi.org/10.1007/s12275-012-1326-z
- 272 View
- 0 Download
- 5 Crossref
-
Abstract
PDF
- Replacement of glycine 227 in the fifth WD40 motif of α-COP/Ret1p/Soo1p by charged or aromatic amino acids is responsible for the temperature-dependent osmo-sensitivity of Saccharomyces cerevisiae, while truncations of WD40 motifs exerted a reduction in cell growth rate and impairment in assembly of cell-wall associated proteins such as enolase and Gas1p. Yeast two-hybrid analysis revealed that the ret1-1/soo1-1 mutation of α-COP abolished the interaction with β- and ε-COP, respectively, and that the interaction between α-COP and β-COP relied on the WD40 domain of α-COP. Furthermore, although the WD40 domain is dispensable for interaction of α-COP with ε-COP, structural alterations in the WD40 domain could impair the interaction.
-
Citations
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Jingjing Zhao, Qian Wang, Xin Ni, Shaonian Shen, Chenchen Nan, Xianzhen Li, Xiaoyi Chen, Fan Yang
Bioresources and Bioprocessing.2022;[Epub] CrossRef - Transcriptional Regulation of Anthocyanin Synthesis by MYB-bHLH-WDR Complexes in Kiwifruit (Actinidia chinensis)
Yanfei Liu, Kangxun Ma, Yingwei Qi, Guowen Lv, Xiaolin Ren, Zhande Liu, Fengwang Ma
Journal of Agricultural and Food Chemistry.2021; 69(12): 3677. CrossRef - Identification and expression profile of an alpha-COPI homologous gene (COPA1) involved in high irradiance and salinity stress in Haematococcus pluvialis
Qiulan Luo, Jingjing Ning, Zhangli Hu, Chaogang Wang
Algal Research.2017; 28: 220. CrossRef - Depletion of ε-COP in the COPI Vesicular Coat Reduces Cleistothecium Production inAspergillus nidulans
Eun-Hye Kang, Eun-Jung Song, Jun Ho Kook, Hwan-Hee Lee, Bo-Ri Jeong, Hee-Moon Park
Mycobiology.2015; 43(1): 31. CrossRef - Analysis of Protein Domain for Interaction between α-COP and ε-COP in Aspergillus nidulans
Eun-Jung Song, Ki-Hyun Kim, Hwan-Hee Lee, Jeong-Seok Park, Eun-Hye Kang, Hee-Moon Park
The Korean Journal of Mycology.2012; 40(4): 224. CrossRef
- Dissecting the essential role of N-glycosylation in catalytic performance of xanthan lyase
- Saccharomyces cerevisiae Cmr1 Protein Preferentially Binds to UV-Damaged DNA In Vitro
- Do-Hee Choi , Sung-Hun Kwon , Joon-Ho Kim , Sung-Ho Bae
- J. Microbiol. 2012;50(1):112-118. Published online February 27, 2012
- DOI: https://doi.org/10.1007/s12275-012-1597-4
- 246 View
- 0 Download
- 10 Crossref
-
Abstract
PDF
-
DNA metabolic processes such as DNA replication, recombination,
and repair are fundamentally important for the
maintenance of genome integrity and cell viability. Although
a large number of proteins involved in these pathways have
been extensively studied, many proteins still remain to be
identified. In this study, we isolated DNA-binding proteins
from Saccharomyces cerevisiae using DNA-cellulose columns.
By analyzing the proteins using mass spectrometry, an uncharacterized
protein, Cmr1/YDL156W, was identified. Cmr1
showed sequence homology to human Damaged-DNA binding
protein 2 in its C-terminal WD40 repeats. Consistent
with this finding, the purified recombinant Cmr1 protein
was found to be intrinsically associated with DNA-binding
activity and exhibited higher affinity to UV-damaged DNA
substrates. Chromatin isolation experiments revealed that
Cmr1 localized in both the chromatin and supernatant
fractions, and the level of Cmr1 in the chromatin fraction
increased when yeast cells were irradiated with UV. These
results
suggest that Cmr1 may be involved in DNA-damage responses in yeast. -
Citations
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Xingyu Liu, Ying Zhang, Zhihui Wen, Yan Hao, Charles A.S. Banks, Joseph Cesare, Saikat Bhattacharya, Shreyas Arvindekar, Jeffrey J. Lange, Yixuan Xie, Benjamin A. Garcia, Brian D. Slaughter, Jay R. Unruh, Shruthi Viswanath, Laurence Florens, Jerry L. Work
Proceedings of the National Academy of Sciences.2024;[Epub] CrossRef - Histone H4 dosage modulates DNA damage response in the pathogenic yeast Candida glabrata via homologous recombination pathway
Kundan Kumar, Romila Moirangthem, Rupinder Kaur, Michael Freitag
PLOS Genetics.2020; 16(3): e1008620. CrossRef - Genome Wide Analysis of WD40 Proteins in Saccharomyces cerevisiae and Their Orthologs in Candida albicans
Buddhi Prakash Jain
The Protein Journal.2019; 38(1): 58. CrossRef - Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
Gerald Dayebgadoh, Mihaela E. Sardiu, Laurence Florens, Michael P. Washburn
Journal of Proteome Research.2019; 18(9): 3479. CrossRef - Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance
Sonia Silva, Veronika Altmannova, Sarah Luke-Glaser, Peter Henriksen, Irene Gallina, Xuejiao Yang, Chunaram Choudhary, Brian Luke, Lumir Krejci, Michael Lisby
Genes & Development.2016; 30(6): 700. CrossRef - Recruitment of Saccharomyces cerevisiae Cmr1/Ydl156w to Coding Regions Promotes Transcription Genome Wide
Jeffery W. Jones, Priyanka Singh, Chhabi K. Govind, Sukesh R. Bhaumik
PLOS ONE.2016; 11(2): e0148897. CrossRef - Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control
Irene Gallina, Camilla Colding, Peter Henriksen, Petra Beli, Kyosuke Nakamura, Judith Offman, David P. Mathiasen, Sonia Silva, Eva Hoffmann, Anja Groth, Chunaram Choudhary, Michael Lisby
Nature Communications.2015;[Epub] CrossRef - Compartment‐specific aggregases direct distinct nuclear and cytoplasmic aggregate deposition
Stephanie BM Miller, Chi‐Ting Ho, Juliane Winkler, Maria Khokhrina, Annett Neuner, Mohamed YH Mohamed, D Lys Guilbride, Karsten Richter, Michael Lisby, Elmar Schiebel, Axel Mogk, Bernd Bukau
The EMBO Journal.2015; 34(6): 778. CrossRef - Yeast gene CMR1/YDL156W is consistently co-expressed with genes participating in DNA-metabolic processes in a variety of stringent clustering experiments
Basel Abu-Jamous, Rui Fa, David J. Roberts, Asoke K. Nandi
Journal of The Royal Society Interface.2013; 10(81): 20120990. CrossRef - Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress
Johnny M. Tkach, Askar Yimit, Anna Y. Lee, Michael Riffle, Michael Costanzo, Daniel Jaschob, Jason A. Hendry, Jiongwen Ou, Jason Moffat, Charles Boone, Trisha N. Davis, Corey Nislow, Grant W. Brown
Nature Cell Biology.2012; 14(9): 966. CrossRef
- An integrated structural model of the DNA damage-responsive H3K4me3 binding WDR76:SPIN1 complex with the nucleosome
- Dynamical Analysis of Yeast Protein Interaction Network During the Sake Brewing Process
- Mitra Mirzarezaee , Mehdi Sadeghi , Babak N. Araabi
- J. Microbiol. 2011;49(6):965-973. Published online December 28, 2011
- DOI: https://doi.org/10.1007/s12275-011-1194-y
- 301 View
- 0 Download
- 1 Crossref
-
Abstract
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- Proteins interact with each other for performing essential functions of an organism. They change partners to get involved in various processes at different times or locations. Studying variations of protein interactions within a specific process would help better understand the dynamic features of the protein interactions and their functions. We studied the protein interaction network of Saccharomyces cerevisiae (yeast) during the brewing of Japanese sake. In this process, yeast cells are exposed to several stresses. Analysis of protein interaction networks of yeast during this process helps to understand how protein interactions of yeast change during the sake brewing process. We used gene expression profiles of yeast cells for this purpose. Results of our experiments revealed some characteristics and behaviors of yeast hubs and non-hubs and their dynamical changes during the brewing process. We found that just a small portion of the proteins (12.8 to 21.6%) is responsible for the functional changes of the proteins in the sake brewing process. The changes in the number of edges and hubs of the yeast protein interaction networks increase in the first stages of the process and it then decreases at the final stages.
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Citations
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Quan Li, Hai-Feng Chen
RSC Advances.2018; 8(24): 13067. CrossRef
- Synergistic regulation mechanism of iperoxo and LY2119620 for muscarinic acetylcholine M2 receptor
- Adaptive Stress Response to Menadione-Induced Oxidative Stress in Saccharomyces cerevisiae KNU5377
- Il-Sup Kim , Ho-Yong Sohn , Ingnyol Jin
- J. Microbiol. 2011;49(5):816-823. Published online November 9, 2011
- DOI: https://doi.org/10.1007/s12275-011-1154-6
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Abstract
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- The molecular mechanisms involved in the ability of yeast cells to adapt and respond to oxidative stress are of great interest to the pharmaceutical, medical, food, and fermentation industries. In this study, we investigated the time-dependent, cellular redox homeostasis ability to adapt to menadione-induced oxidative stress, using biochemical and proteomic approaches in Saccharomyces cerevisiae KNU5377. Time-dependent cell viability was inversely proportional to endogenous amounts of ROS measured by a fluorescence assay with 2′,7′-dichlorofluorescin diacetate (DCFHDA), and was hypersensitive when cells were exposed to the compound for 60 min. Morphological changes, protein oxidation and lipid peroxidation were also observed. To overcome the unfavorable conditions due to the presence of menadione, yeast cells activated a variety of cell rescue proteins including antioxidant enzymes, molecular chaperones, energy-generating metabolic enzymes, and antioxidant molecules such as trehalose. Thus, these results show that menadione causes ROS generation and high accumulation of cellular ROS levels, which affects cell viability and cell morphology and there is a correlation between resistance to menadione and the high induction of cell rescue proteins after cells enter into this physiological state, which provides a clue about the complex and dynamic stress response in yeast cells.
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Citations
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Rasa Garjonyte, Vytautas Melvydas, Albertas Malinauskas
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Shiro Yamashoji
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José Antonio Cervantes-Chávez, Laura Valdés-Santiago, Guus Bakkeren, Edda Hurtado-Santiago, Claudia Geraldine León-Ramírez, Edgardo Ulises Esquivel-Naranjo, Fidel Landeros-Jaime, Yolanda Rodríguez-Aza, José Ruiz-Herrera
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Yulia A. Sidorova, Maria L. Perepechaeva, Elena N. Pivovarova, Arkady L. Markel, Vyacheslav V. Lyakhovich, Alevtina Y. Grishanova, Pratibha V. Nerurkar
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Open Life Sciences.2014; 9(2): 173. CrossRef - Genome-wide RNAi screen reveals the E3 SUMO-protein ligase gene SIZ1 as a novel determinant of furfural tolerance in Saccharomyces cerevisiae
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Polar Biology.2013; 36(3): 381. CrossRef
- Galactose Inhibits the Translation of Erg1 that Enhances the Antifungal Activities of Azoles Against Candida albicans
- Response of Saccharomyces cerevisiae to Stress-Free Acidification
- Allen Kuan-Liang Chen , Cristy Gelling , Peter L. Rogers , Ian W. Dawes , Bettina Rosche
- J. Microbiol. 2009;47(1):1-8. Published online February 20, 2009
- DOI: https://doi.org/10.1007/s12275-008-0167-2
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- 18 Crossref
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Abstract
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- Genome-wide transcriptional analysis of a Saccharomyces cerevisiae batch culture revealed that more than 829 genes were regulated in response to an environmental shift from pH 6 to pH 3 by added sulfuric acid. This shift in pH was not detrimental to the rate of growth compared to a control culture that was maintained at pH 6 and the transcriptional changes most strikingly implicated not up- but down-regulation of stress responses. In addition, the transcriptional changes upon acid addition indicated remodeling of the cell wall and central carbon metabolism. The overall trend of changes was similar for the pH-shift experiment and the pH 6 control. However, the changes in the pH 6 control were much weaker and occurred 2.5 h later than in the pH-shift experiment. Thus, the reaction to the steep pH decrease was an immediate response within the normal repertoire of adaptation shown in later stages of fermentation at pH 6. Artificially preventing the yeast from acidifying the medium may be considered physiologically stressful under the tested conditions.
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Citations
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Ghaneshree Moonsamy, Sarisha Singh, Yrielle Roets-Dlamini, Koketso Kenneth Baikgaki, Santosh Omrajah Ramchuran
Fermentation.2025; 11(4): 186. CrossRef - Isolation of yeast from some Ethiopian traditional fermented beverages and in vitro evaluation for probiotic traits
Dagnew Bitew, Bogale Damtew, Anteneh Tesfaye, Berhanu Andualem
Heliyon.2024; 10(23): e40520. CrossRef - Assessment of Yeasts as Potential Probiotics: A Review of Gastrointestinal Tract Conditions and Investigation Methods
Nadia S. Alkalbani, Tareq M. Osaili, Anas A. Al-Nabulsi, Amin N. Olaimat, Shao-Quan Liu, Nagendra P. Shah, Vasso Apostolopoulos, Mutamed M. Ayyash
Journal of Fungi.2022; 8(4): 365. CrossRef - Microbiological and biochemical performances of six yeast species as potential starter cultures for wet fermentation of coffee beans
Hosam Elhalis, Julian Cox, Damian Frank, Jian Zhao
LWT.2021; 137: 110430. CrossRef - Bioconversion and valorization of cassava-based industrial wastes to bioethanol gel and its potential application as a clean cooking fuel
Andin Vita Amalia, Fidia Fibriana, Talitha Widiatningrum, Risa Dwita Hardianti
Biocatalysis and Agricultural Biotechnology.2021; 35: 102093. CrossRef - QTL mapping of a Brazilian bioethanol strain links the cell wall protein-encoding gene GAS1 to low pH tolerance in S. cerevisiae
Alessandro L. V. Coradini, Fellipe da Silveira Bezerra de Mello, Monique Furlan, Carla Maneira, Marcelo F. Carazzolle, Gonçalo Amarante Guimaraes Pereira, Gleidson Silva Teixeira
Biotechnology for Biofuels.2021;[Epub] CrossRef - Extreme Low Cytosolic pH Is a Signal for Cell Survival in Acid Stressed Yeast
Rodrigo Mendonça Lucena, Laura Dolz-Edo, Stanley Brul, Marcos Antonio de Morais, Gertien Smits
Genes.2020; 11(6): 656. CrossRef - The effects of starvation and acidification on lag phase duration of surviving yeast cells
Kenichi Shibata, Kohei Obase, Kiminori Itoh, Takashi Amemiya
Journal of Biotechnology.2018; 275: 60. CrossRef - Neutralization of acidic drainage by Cryptococcus sp. T1 immobilized in alginate beads
Masahiko Okai, Chisato Suwa, Shintaro Nagaoka, Nobuo Obara, Daisuke Mitsuya, Ayako Kurihara, Masami Ishida, Naoto Urano
Bioscience, Biotechnology, and Biochemistry.2017; 81(11): 2216. CrossRef - Genetic Interaction between HOG1 and SLT2 Genes in Signalling the Cellular Stress Caused by Sulphuric Acid in Saccharomyces cerevisiae
Rodrigo Mendonça de Lucena, Carolina Elsztein, Rafael Barros de Souza, Will de Barros Pita, Sérgio de Sá Leitão Paiva Jr., Marcos Antonio de Morais Jr.
Microbial Physiology.2015; 25(6): 423. CrossRef - Transcriptomic response of Saccharomyces cerevisiae for its adaptation to sulphuric acid-induced stress
Rodrigo Mendonça de Lucena, Carolina Elsztein, Will de Barros Pita, Rafael Barros de Souza, Sérgio de Sá Leitão Paiva Júnior, Marcos Antonio de Morais Junior
Antonie van Leeuwenhoek.2015; 108(5): 1147. CrossRef - RNA-seq analysis of Pichia anomala reveals important mechanisms required for survival at low pH
Eugene Fletcher, Amir Feizi, SungSoo Kim, Verena Siewers, Jens Nielsen
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Rogelio Lopes Brandão, Júlio César Câmara Rosa, Jacques Robert Nicoli, Marcos Vinicius Simi Almeida, Ana Paula do Carmo, Heloa Teixeira Queiros, Ieso Miranda Castro
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R.M. Lucena, C. Elsztein, D.A. Simões, M.A. Morais
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Flávia Lima do Carmo, Henrique Fragoso dos Santos, Edir Ferreira Martins, Jan Dirk van Elsas, Alexandre Soares Rosado, Raquel Silva Peixoto
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- Development of a High-Cell-Density Production Process for a Biotherapeutic Yeast, Saccharomyces cerevisiae var. boulardii, for Use as a Human Probiotic
Journal Article
- The Physiological Role of CPR1 in Saccharomyces cerevisiae KNU5377 against Menadione Stress by Proteomics
- Il Sup Kim , Hae Sun Yun , Sun Hye Kwak , Ing Nyol Jin
- J. Microbiol. 2007;45(4):326-332.
- DOI: https://doi.org/2565 [pii]
- 195 View
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Abstract
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- In order to understand the functional role of CPR1 in Saccharomyces cerevisiae KNU5377 with regard to its multi-tolerance characteristics against high temperatures, inorganic acids, and oxidative stress conditions, whole cellular proteins were analyzed via liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). This procedure was followed by two-dimensional (2-D) gel electrophoresis. Under menadione stress conditions, the 23 upregulated proteins were clearly identified only in the wildtype strain of KNU5377. Among the proteins, Sod1p, Tsa1p, Ahp1, Cpr1p, Cpr3, Ssb2p, and Hsp12p were identified as components of antioxidant systems or protein-folding related systems. The CPR1 protein could not be completely detected in the cpr1Δ mutant of KNU5377 and the other upregulated proteins in the wild-type strain evidenced a clear correlation with the results of immunoblot analysis. Moreover, a reduction in growth patterns (about 50%) could be observed in the cpr1Δ mutant, as compared with that of the wild-type strain under mild MD stress conditions. These results indicate that the upregulation of CPR1 may contribute to tolerance against MD as an inducer of oxidative stress.
Research Support, Non-U.S. Gov'ts
- Heat Shock Causes Oxidative Stress and Induces a Variety of Cell Rescue Proteins in Saccharomyces cerevisiae KNU5377
- Il-Sup Kim , Hye-Youn Moon , Hae-Sun Yun , Ingnyol Jin
- J. Microbiol. 2006;44(5):492-501.
- DOI: https://doi.org/2449 [pii]
- 213 View
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Abstract
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- In this study, we attempted to characterize the physiological response to oxidative stress by heat shock in Saccharomyces cerevisiae KNU5377 (KNU5377) that ferments at a temperature of 40°C. The KNU5377 strain evidenced a very similar growth rate at 40°C as was recorded under normal conditions. Unlike the laboratory strains of S. cerevisiae, the cell viability of KNU5377 was affected slightly under 2 hours of heat stress conditions at 43°C. KNU5377 evidenced a time-dependent increase in hydroperoxide levels, carbonyl contents, and malondialdehyde (MDA), which increased in the expression of a variety of cell rescue proteins containing Hsp104p, Ssap, Hsp30p, Sod1p, catalase, glutathione reductase, G6PDH, thioredoxin, thioredoxin peroxidase (Tsa1p), Adhp, Aldp, trehalose and glycogen at high temperature. Pma1/2p, Hsp90p and H+-ATPase expression levels were reduced as the result of exposure to heat shock. With regard to cellular fatty acid composition, levels of unsaturated fatty acids (USFAs) were increased significantly at high temperatures (43°C), and this was particularly true of oleic acid (C18:1). The results of this study indicated that oxidative stress as the result of heat shock may induce a more profound stimulation of trehalose, antioxidant enzymes, and heat shock proteins, as well as an increase in the USFAs ratios. This might contribute to cellular protective functions for the maintenance of cellular homeostasis, and may also contribute to membrane fluidity.
- Sterilization of Bacteria, Yeast, and Bacterial Endospores by Atmospheric-Pressure Cold Plasma using Helium and Oxygen
- Kyenam Lee , Kwang-hyun Paek , Won-Tae Ju , Yoenhee Lee
- J. Microbiol. 2006;44(3):269-275.
- DOI: https://doi.org/2386 [pii]
- 217 View
- 1 Download
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Abstract
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- Atmospheric-pressure cold plasma (APCP) using helium/oxygen was developed and tested as a suitable sterilization method in a clinical environment. The sterilizing effect of this method is not due to UV light, which is known to be the major sterilization factor of APCP, but instead results from the action of reactive oxygen radicals. Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae deposited on a nitrocellulose filter membrane or Bacillus subtilis spores deposited on polypropylene plates were exposed to helium/oxygen plasma generated with AC input power at 10 kHz, 6 kV. After plasma treatment, nitrocellulose filter membranes were overlaid on fresh solid media and CFUs were counted after incubation overnight. D-values were 18 sec for E. coli, 19 sec for S. aureus, 1 min 55 sec for S. cerevisiae, and 14 min for B. subtilis spores. D-values of bacteria and yeast were dependent on the initial inoculation concentration, while the D-value of B. subtilis spores showed no correlation. When treated cells were observed with a scanning electron microscope, E. coli was more heavily damaged than S. aureus, S. cerevisiae exhibited peeling, and B. subtilis spores exhibited shrunken morphology. Results showed that APCP using helium/oxygen has many advantages as a sterilization method, especially in a clinical environment with conditions such as stable temperature, unlimited sample size, and no harmful gas production.
- Role of RNA Polymerase II Carboxy Terminal Domain Phosphorylation in DNA Damage Response
- Su-Jin Jeong , Hye-Jin Kim , Yong-Jin Yang , Ja-Hwan Seol , Bo-Young Jung , Jeong-Whan Han , Hyang-Woo Lee , Eun-Jung Cho
- J. Microbiol. 2005;43(6):516-522.
- DOI: https://doi.org/2296 [pii]
- 189 View
- 0 Download
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Abstract
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- The phosphorylation of C-terminal domain (CTD) of Rpb1p, the largest subunit of RNA polymerase II plays an important role in transcription and the coupling of various cellular events to transcription. In this study, its role in DNA damage response is closely examined in Saccharomyces cerevisiae, focusing specifically on several transcription factors that mediate or respond to the phosphorylation of the CTD. CTDK-1, the pol II CTD kinase, FCP1, the CTD phosphatase, ESS1, the CTD phosphorylation dependent cis-trans isomerase, and RSP5, the phosphorylation dependent pol II ubiquitinating enzyme, were chosen for the study. We determined that the CTD phosphorylation of CTD, which occurred predominantly at serine 2 within a heptapeptide repeat, was enhanced in response to a variety of sources of DNA damage. This modification was shown to be mediated by CTDK-1. Although mutations in ESS1 or FCP1 caused cells to become quite sensitive to DNA damage, the characteristic pattern of CTD phosphorylation remained unaltered, thereby implying that ESS1 and FCP1 play roles downstream of CTD phosphorylation in response to DNA damage. Our data suggest that the location or extent of CTD phosphorylation might be altered in response to DNA damage, and that the modified CTD, ESS1, and FCP1 all contribute to cellular survival in such conditions.
- Complete Sequence of a Gene Encoding KAR3-Related Kinesin-like Protein in Candida albicans
- Min-Kyoung Kim , Young Mi Lee , Wankee Kim , Wonja Choi
- J. Microbiol. 2005;43(5):406-410.
- DOI: https://doi.org/2283 [pii]
- 191 View
- 0 Download
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Abstract
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- In contrast to Saccharomyces cerevisiae, little is known about the kinesin-like protein (KLP) in Candida albicans. The motor domain of kinesin, or KLP, contains a subregion, which is well conserved from yeast to humans. A similarity search, with the murine ubiquitous kinesin heavy chain region as a query, revealed 6 contigs that contain putative KLPs in the genome of C. albicans. Of these, the length of an open reading (ORF) of 375 amino acids, temporarily designated CaKAR3, was noticeably short compared with the closely related S. cerevisiae KAR3 (ScKAR3) of 729 amino acids. This finding prompted us to isolate a [lambda] genomic clone containing the complete CaKAR3 ORF, and here the complete sequence of CaKAR3 is reported. CaKAR3 is a C-terminus motor protein, of 687 amino acids, encoded by a non-disrupting gene. When compared with ScKAR3, the amino terminal region of 112 amino acids was unique, with the middle part of the 306 amino acids exhibiting 25% identity and 44% similarity, while the remaining C-terminal motor domain exhibited 64% identity and 78% similarity, and have been submitted to GeneBank under the accession number AY182242.
Journal Articles
- Expression of Escherichia coli Heat-labile Enterotoxin B Subunit (LTB) in Saccharomyces cerevisiae
- Mohammad Ahangarzadeh Rezaee , Abbas Rezaee , Seyed Mohammad Moazzeni , Ali Hatef Salmanian , Yoko Yasuda , Kunio Tochikubo , Shahin Najar Pirayeh , Mohsen Arzanlou
- J. Microbiol. 2005;43(4):354-360.
- DOI: https://doi.org/2254 [pii]
- 233 View
- 0 Download
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Abstract
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- Heat-labile enterotoxin B subunit (LTB) of enterotoxigenic Escherichia coli (ETEC) is both a strong mucosal adjuvant and immunogen. It is a subunit vaccine candidate to be used against ETEC-induced diarrhea. It has already been expressed in several bacterial and plant systems. In order to construct yeast expressing vector for the LTB protein, the eltB gene encoding LTB was amplified from a human origin enterotoxigenic E. coli DNA by PCR. The expression plasmid pLTB83 was constructed by inserting the eltB gene into the pYES2 shuttle vector immediately downstream of the GAL1 promoter. The recombinant vector was transformed into S. cerevisiae and was then induced by galactose. The LTB protein was detected in the total soluble protein of the yeast by SDS-PAGE analysis. Quantitative ELISA showed that the maximum amount of LTB protein expressed in the yeast was approximately 1.9% of the total soluble protein. Immunoblotting analysis showed the yeast-derived LTB protein was antigenically indistinguishable from bacterial LTB protein. Since the whole-recombinant yeast has been introduced as a new vaccine formulation the expression of LTB in S. cerevisiae can offer an inexpensive yet effective strategy to protect against ETEC, especially in developing countries where it is needed most.
- Regulation of Branched-Chain, and Sulfur-Containing Amino Acid Metabolism by Glutathione during Ultradian Metabolic Oscillation of Saccharomyces cerevisiae
- Ho-Yong Sohn , Eun-Joo Kum , Gi-Seok Kwon , Ingnyol Jin , Hiroshi Kuriyama
- J. Microbiol. 2005;43(4):375-380.
- DOI: https://doi.org/2250 [pii]
- 194 View
- 0 Download
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Abstract
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- Autonomous ultradian metabolic oscillation (T~=50 min) was detected in an aerobic chemostat culture of Saccharomyces cerevisiae. A pulse injection of GSH (a reduced form of glutathione) into the culture induced a perturbation in metabolic oscillation, with respiratory inhibition caused by H_2S burst production. As the production of H_2S in the culture was controlled by different amino acids, we attempted to characterize the effects of GSH on amino acid metabolism, particularly with regard to branched chain and sulfur-containing amino acids. During stable metabolic oscillation, concentrations of intracellular glutamate, aspartate, threonine, valine, leucine, isoleucine, and cysteine were observed to oscillate with the same periods of dissolved O_2 oscillation, although the oscillation amplitudes and maximal phases were shown to differ. The methionine concentration was stably maintained at 0.05 mM. When GSH (100 uM) was injected into the culture, cellular levels of branched chain amino acids increased dramatically with continuous H_2S production, whereas the cysteine and methionine concentrations were noticeably reduced. These results indicate that GSH-dependent perturbation occurs as the result of the promotion of branched chain amino acid synthesis and an attenuation of cysteine and methionine synthesis, both of which activate the generation of H_2S. In a low sulfate medium containing 2.5 mM sulfate, the GSH injections did not result in perturbations of dissolved O_2, NAD(P)H redox oscillations without burst H_2S production. This suggests that GSH-dependent perturbation is intimately linked with the metabolism of branched-chain amino acids and H_2S generation, rather than with direct GSH-GSSG redox control.
Research Support, Non-U.S. Gov'ts
- Enhanced Secretion of Cell Wall Bound Enolase into Culture Medium by the soo1-1 Mutation of Saccharomyces cerevisiae
- Ki-Hyun Kim , Hee-Moon Park
- J. Microbiol. 2004;42(3):248-252.
- DOI: https://doi.org/2080 [pii]
- 180 View
- 0 Download
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Abstract
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- In order to identify the protein(s) secreted into culture medium by the soo1-1/ret1-1 mutation of Saccharomyces cerevisiae, proteins from the culture medium of cells grown at permissive (28^oC) and non-permissive temperatures (37^oC), were analyzed. Comparison of protein bands separated by SDS-PAGE identified a prominent band of 47-kDa band from a mutant grown at 37^oC. N-terminal amino acid sequencing of this 47-kDa protein showed high identity with enolases 1 and 2. Western blot analysis revealed that most of the cell wall-bound enolase was released into the culture medium of the mutant grown at 37^oC, some of which were separated as those with lower molecular weights. Our results, presented here, indicate the impairment of cell wall enolase biogenesis and assembly by the soo1-1/ret1-1 mutation of S. cerevisiae.
- Optimal Fermentation Conditions for Enhanced Glutathione Production by Saccharomyces cerevisiae FF-8
- Jae-Young Cha , Jin-Chul Park , Beong-Sam Jeon , Young-Choon Lee , Young-Su Cho
- J. Microbiol. 2004;42(1):51-55.
- DOI: https://doi.org/2000 [pii]
- 293 View
- 2 Download
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Abstract
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- The influence of feedstock amino acids, salt, carbon and nitrogen sources on glutathione production by Saccharomyces cerevisiae FF-8 was investigated. Glucose, yeast extract, KH_2PO_4, and L-cysteine were found to be suitable feedstock. Highest glutathione production was obtained after cultivation with shaking for 72 h in a medium containing glucose 3.0% (w/v), yeast extract 3.0%, KH_2PO_4 0.06% and L-cysteine 0.06%. The glutathione concentration achieved using this medium increased 2.27-fold to 204 mg/l compared to YM basal medium.
- The Mutation of a Novel Saccharomyces cerevisiae SRL4 Gene Rescues the Lethality of rad53 and lcd1 Mutations by Modulating dNTP Levels
- Do-Hee Choi , Young-Mi Oh , Sung-Hun Kwon , Sung-Ho Bae
- J. Microbiol. 2008;46(1):75-80.
- DOI: https://doi.org/10.1007/s12275-008-0013-6
- 295 View
- 0 Download
- 5 Crossref
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Abstract
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- The SRL4 (YPL033C) gene was initially identified by the screening of Saccharomyces cerevisiae genes that play a role in DNA metabolism and/or genome stability using the SOS system of Escherichia coli. In this study, we found that the srl4Δ; mutant cells were resistant to the chemicals that inhibit nucleotide metabolism and evidenced higher dNTP levels than were observed in the wild-type cells in the presence of hydroxyurea. The mutant cells also showed a significantly faster growth rate and higher dNTP levels at low temperature (16 oC) than were observed in the wild-type cells, whereas we detected no differences in the growth rate at 30oC. Furthermore, srl4Δ was shown to suppress the lethality of mutations of the essential S phase checkpoint genes, RAD53 and LCD1. These results indicate that SRL4 may be involved in the regulation of dNTP production by its function as a negative regulator of ribonucleotide reductase.
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Citations
Citations to this article as recorded by- Peroxisomal Compartmentalization of the Methylerythritol-4-phosphate Pathway Alleviates Cellular Stress and Enhances Geraniol Production in Saccharomyces cerevisiae
Jerome R. Lon, Xuemei Zhao, Gulkiz Mamatrixat, Zhoukang Zhuang, Zhehao Jin, Tao Yu, Jufang Wang, Hongting Tang
ACS Synthetic Biology.2025; 14(8): 3037. CrossRef - Chromosome-wide histone deacetylation by sirtuins prevents hyperactivation of DNA damage-induced signaling upon replicative stress
Antoine Simoneau, Étienne Ricard, Sandra Weber, Ian Hammond-Martel, Lai Hong Wong, Adnane Sellam, Guri Giaever, Corey Nislow, Martine Raymond, Hugo Wurtele
Nucleic Acids Research.2016; 44(6): 2706. CrossRef - Saccharomyces cerevisiae Cmr1 protein preferentially binds to UV-damaged DNA in vitro
Do-Hee Choi, Sung-Hun Kwon, Joon-Ho Kim, Sung-Ho Bae
The Journal of Microbiology.2012; 50(1): 112. CrossRef - The checkpoint transcriptional response: Make sure to turn it off once you are satisfied
Marcus B. Smolka, Francisco M. Bastos de Oliveira, Michael R. Harris, Robertus A.M. de Bruin
Cell Cycle.2012; 11(17): 3166. CrossRef - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe
Dong-Uk Kim, Jacqueline Hayles, Dongsup Kim, Valerie Wood, Han-Oh Park, Misun Won, Hyang-Sook Yoo, Trevor Duhig, Miyoung Nam, Georgia Palmer, Sangjo Han, Linda Jeffery, Seung-Tae Baek, Hyemi Lee, Young Sam Shim, Minho Lee, Lila Kim, Kyung-Sun Heo, Eun Joo
Nature Biotechnology.2010; 28(6): 617. CrossRef
- Peroxisomal Compartmentalization of the Methylerythritol-4-phosphate Pathway Alleviates Cellular Stress and Enhances Geraniol Production in Saccharomyces cerevisiae
- Overexpression of the SPP2 Gene of Saccharomyces cerevisiae and Production of Antibodiesd to Spp2p
- Park, Kwang Hark , Lea, Ho Zoo , Woolford, John L. , Kim, Kyung Hoon
- J. Microbiol. 1995;33(3):201-207.
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- We have previously reported that SPP2 gene product of yeast Saccharomyces cerevisiae is involved in the pre-mRNA splicing. To investigate the rol ein the splicing pathway of the Spp2p protein, the SPP2 gene was overexpressed in Escherichia coli and polyclonal antibodies to Spp2p were generated from rabbits. First, a DNA fragment containing the SPP2 GENE without its promoter was subcloned into an E. coli expression vector, pKK233-3. The resulting recombinant plasmid pBQ14 contained an IPTG inducible tac promoter and the SPP2 structural gene. Overexpression of the SPP2 gene was achieved by additionof 0.1 to 1.0 mM IPTG to a logarithmic culture of E. coli JM103(pBQ14) for 90 min at 37℃. Sequence of N-terminal 15 amino acids of the overproduced protein was well matched to the deduced one from the SPP2 reading frame. Then, polyclonal antibodies were generated from rabbits immunized with gel-purified Spp2p protein. These antibodies reacted specifically with the Spp2p protein extracted from yeast cells expressing the SPP2 gene to a great extent. The antibodies could also block the activity of yeast splicing extracts.
- Induced Level of CIN2 Gene Transcripts in yeast by Cycloheximide Treatment
- Lee , Myeong Sok
- J. Microbiol. 1995;33(1):46-50.
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- The interrelationship of expression of the two adjacent genes, HSP82 and CIN2 in Saccharomyces cerevisiae was investigated. The two genes are very close each other, such that the poly(A) addition site of the HSP82 gene is likely to be immediately followed by the promoter region of the CIN2 gene. Thus, transcription of the upstream HSP82 gene might affect expression of the CIN2 gene via promoter occlusion. Based on HSP82 and CIN2 mRNA analysis of the wild type, disruption mutant of the HAP82 gene, and conditional RNA polymerase II mutant strains, I conclude that CIN2 transcription is not affected by HSP82 transcription. However, surprisingly the level of CIN2 gene transcripts, but not HSP82, is heightened by cycloheximide treatment, presumably due to an increase in its stability. No change is observed in kinetics of HSP82 RNA induction by cycloheximide treatment.
- DNA Replication is not Required in Re-establishment of HMRE Silencer Function at the HSP82 Yeast Heat Shock Locus
- Lee, See Woo , Gross, David S.
- J. Microbiol. 1996;34(1):30-36.
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- We have examined the re-establishment of HIMRE mediated silencing function on the transcriptional activity of yeast heat shock gene HSP82. To test whether the onset of SIR repression can occur in growing cells in the presence of a potent inhibitor of DNA replication, HMRa/HSP82 strains with SIR4^- and SIR4S^+ genetic backgrounds were arrested in S phase by incubation of a culture in 200 mM hydroxyurea for 120 min. It was clear that following a 20 minute heat shock, silencing of the HMRa/HSP82 allele in cells pretreated with hydroxyurea does occur in a SIR4-dependen fashion, even though the kinetics of repression appears to be substantially delayed. We also have tested whether re- establishment of silencing at the HMR/hsp82 locus can occur in G1-arrested cells. Cell cycle arrest at G1 phase was achieved by treatment of early log a cell cultures with α-factor mating pheromone, which induces G1 arrest. The result suggests that passage through S phase (and therefore DNA replication) is nor required for re-establishing silencer-mediated repression at the HMNRa/HSP82 locus. Finally, to test whether de novo protein synthesis is required for re-establishment of silencer-mediated repression, cells were pretreated with cycloheximide (500 ㎍/㎖) 120 min. It was apparent that inhibiting protein synthesis delays, but does not prevent, re-establishment of silencer-mediated repression. Altogether, these results indicate that re-establishment of silencer-mediated repression is not dependent on the DNA replication and has no requirement for protein synthesis.
- Reorganization of chromatin conformation from an active to an inactive state after cessation of transcription
- Lee , Myeong Sok
- J. Microbiol. 1996;34(1):54-60.
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- Taking advantage of the heat inducible HSP82 gene in yeast, chromatin structure after transcription cessation was investigated. Alteration of chromating conformation within the HSP82 gene transcription unit into an active state has been shown to correlate with its transcriptional induction. It was thus of interest to examine whether the active chromatin state within the HSP82 mRNA analysis, the gene ceased its transcription within a few hours of cultivation at a normal condition after heat induction. In this condition, an active chromatin conformation in the HSP82 gene body was changed into an inactive state which was revealed by DNase I resistance and by typical nucleosomal cutting periodicity in the corresponding chromatin. These results thus ruled out the possibility of a long-term maintenance of the DNase I sensitive chromatin after transcription cessation. DNA replication may be a critical event for the chromatin reprogramming.
- Immunofluorescence localization of schizosacharomjyces pombe cdc103^+ gene product
- Kim , Hyong Bai
- J. Microbiol. 1996;34(3):248-254.
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- cdc103^+ gene in Schizosaccharomyces pombe which is similar to the CDC3 gene in Saccharomyces cerevisiae was cloned and sequenced. Comparison of the predicted amino acid sequences of cdc103^+ and CDC3 revealed that they share significant similarity (43% identity and 56% identity or similarity) to each other. The gene product of CDC3 S. cerevisiae is known to be a highly ordered ring of filaments that lies just inside the cytoplasmic membrane in the region of the mother-bud neck. In order to characterize the gene product of cdc103^+ in Schizosaccharomyces pombe, fusion proteins were used to generate the polyclonal antibodies specific for the gene product (cdc103p). In immunofluorescence experiments, these antibodies decorate the region of the septum formation as a double ring structure late in the cell division cycle.
- High Dosage of Rok1p, a Putative ATP-dependent RNA Helicase, Leads to a Cell Cycle Arrest at G1/S Stage in Saccharomyces cerevisiae
- jeong, Hyun Sook , Oh, Jae Young , Kim, Jin Mi
- J. Microbiol. 1998;36(2):139-144.
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- The ROK1 gene encodes a putative ATP-dependent RNA helicase which is essential for mitotic cell growth. ROK1 has been thought to affect microtubule and spindle pole body (SPB) functions in Saccharomyces cerevisiae. To investigate the intracellular functions of ROK1, we varied the Rok1 protein dosage in a cell and analyzed its phenotypic effects. Overexpression of the ROK1 gene by using a strong GAL1 promoter was lethal, leading cells to arrest at the unbudded stage. This arrest phenotype is very similar to that of the rok1 null mutation. Indirect immunofluorescence revealed that the majority of arrested cells contained a single SPB. Normas development of microtubules between the duplicated SPSs was rarely observed. Multinuclear cells with abnormal microtubule array were detected in small fraction. Taken together with the phenotype of the rlk1 null mutation, these results imply that ROK1 is required for cell cycle progression at the G1/S stage.
- A Yeast MRE3/REC114 Gene is Essential for Normal Cell Growth and Meiotic Recombination
- Sun-Hee Leem
- J. Microbiol. 1999;37(4):248-255.
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- We have analyzed the MRE3/REC114 gene of Saccharomyces cerevisiae, previously detected in isolation of mutants defective in meiotic recombination. We cloned the MRE3/REC114 gene by complementation of the meiotic recombination defect and it has been mapped to chormosome XIII. The DNA sequence analysis revealed that the MRE3 gene is identical to the REC114 gene. The upstream region of the MRE3/REC114 gene contains a T_4C site, a URS (upstream repression sequence) and a TR (T-rich) box-like sequence, which reside upstream of many meiotic genes. Coincidentally, northern blot analysis indicated that the three sizes of MRE3/REC114 transcripts, 3.4, 1.4 and 1.2 kb, are induced in meiosis. A less abundant transcript of 1.4 kb is detected in both mitotic and meiotic cells, suggesting that it is needed in mitosis as well as meiosis. To examine the role of the MRE3/REC114 gene, we constructed mre3 disruption mutants. Strains carrying an insertion or null deletion of the MRE3/REC114 gene showed slow growth in nutrient medium and the doubing time of these cells increased approximately by 2-fond compared to the wild-type strain. Moreover, the deletion mutant (delta-mre3) displayed no meiotically induced recombination and no viable spores. The mre3/rec114 spore lethality can be suppressed by spo13, a mutation that causes cells to bypass reductional division. The double-stranded breaks (DSBs) which are involved in initiation of meiotic recombination were not detected in the analysis of meiotic chromosomal DNA from the mre3/rec114 disruptant. From these results we suggest that the MRE3/REC114 gene product is essential in nomal growth and in early meiotic stages involved in meiotic recombination.
- Monascus Red Pigment Overproduction by Coculture with Recombinant Saccharomyces cerevisiae Secreting Glucoamylase
- Ho-Soo Lim , Seung-Ku Yoo , Chul-Soo Shin , Young-Min Hyun
- J. Microbiol. 2000;38(1):48-51.
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- In liquid cultures using sucrose media, the coculture of Monascus with recombinant Saccharomyces cerevisiae expressing the glucoamylase gene from Aspergillus niger enhanced red pigment production by approx. 19%, compared with the coculture of wild type S. cerevisiae. Coculture with recombinant S. cerevisiae was more effective than with wild type S. cerevisiae for Monascus red pigment production. Cocultures of Monascus with commercial amylases of Aspergillus also induced high production of pigment and morphological changes in a solid culture using sucrose media.
- Cloning of Genomic DNAs of Trametes versicolor Acting as Autonomously Replicating Sequences in Saccharomyces cerevisiae
- Sora An , Kyoung Phil Park , Hyoung-Tae Choi , Kyu-Joong Kim , Kyunghoon Kim
- J. Microbiol. 2002;40(3):245-247.
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- A genomic DNA library of the fungus Trametes versicolor was constructed in a yeast integration vector which contains the URA3 gene of the budding yeast Saccharomyces cerevisiae and the gene responsible for hygromycin B resistance, and fragments acting as autonomously replicating sequences (ARSes) in the budding yeast were identified from the genomic DNA library. Sixteen recombinant plasmids from the library transformed the budding yeast Saccharomyces cerevisiae to Ura^+ at high frequencies. They were maintained stably under selective conditions, but were gradually lost from yeast cells at different rates under nonselective conditions, indicating that they contain eukaryotic origins of DNA replication and exist as extrachromosomal plasmids. Base sequences of four ARS DNAs among the 16 cloned fragments revealed that all of the four contain at least one 11 bp [(A/T)TTTA(T/C)(A/G)TTT(A/T)] consensus sequence of the budding yeast ARS.
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