Journal of Microbiology

Sequence analysis of the schizosaccharomycs pombe homologue of the CDC3 gene in saccharomyces cerevisiae: Kim , Hyong Bai; J. Microbiol. 1995;33(4):350-354.

Abstract: Saccharomyces cervisiae has a highly ordered ring of filaments that lies just inside the cytoplasmic membrane in the region of the mother-bud neck. Mutants defective in any one of the our cell division cycle genes (CDC3, CDC10, CDC11, CDC12) fail to form these filaments and exhibit a pleiotropic phenotype that includes failure to complete cytokinesis and abnormal bud growth. However, the role of the filament is not clear. In order to find out the role of filament, the similar gene in S pombe (called cdc103^+) to the CDC3 was cloned and sequenced. Here I report the sequence analysis of the cdc103^+) to the CDC3 was cloned and sequenced. Here I report the sequence analysis of the cdc103^+. Comparison of the predicted amino acid sequences of cdc103^+ and CDC3 revealed that they share significant similarity (43% identity and 56% identity or similarity) to each other.

Detection of L-xylosone and its physiological effects in saccharomyces cerevisiae: Seok, Yeong Jae , Yang, Kap Seok , Kang, Ju Gyeong , Kim, Seong Tae , Huh, Won Ki , Kang, Sa Ouk; J. Microbiol. 1996;34(2):192-197.

Abstract: L-Xylosone was detected as its quinoxaline derivative in the degradation solution of dehydro-L-ascorbic acid. The production rate of L-xylosone was much faster in aerated phosphate-cirate buffer (pH 5. 6) than in pure water. L-Xylosone and dehydro-L-ascorbic acid were identified in the crude extracts of Saccharomyces cerevisiae. The concentration of L-xylosone in the crude cell extracts was calculated to be about 0.2 nmol (mg protein)^-1. When L-xylosone was added to asynchronous culture of S. cerevisiae, it inhibited primarily the synthesis of protein and RNA. Examination of the effect of L- xylosone on synchronous culture of the yeast indicated that L-xylosone inhibited the initiation of DNA replication and that the cells were arrested at G₁stage of cell division cycle. These results suggested a possibility that L-xylosone can act as an inhibitor of cell growth.

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