Journal of Microbiology

Journal Articles

Comparative Transcriptomic Analysis of Flagellar‑Associated Genes in Salmonella Typhimurium and Its rnc Mutant: Seungmok Han , Ji-Won Byun , Minho Lee; J. Microbiol. 2024;62(1):33-48. Published online January 5, 2024; DOI: https://doi.org/10.1007/s12275-023-00099-5

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Salmonella enterica serovar Typhimurium (S. Typhimurium) is a globally recognized foodborne pathogen that affects both animals and humans. Endoribonucleases mediate RNA processing and degradation in the adaptation of bacteria to environmental changes and have been linked to the pathogenicity of S. Typhimurium. Not much is known about the specific regulatory mechanisms of these enzymes in S. Typhimurium, particularly in the context of environmental adaptation. Thus, this study carried out a comparative transcriptomic analysis of wild-type S. Typhimurium SL1344 and its mutant (Δrnc), which lacks the rnc gene encoding RNase III, thereby elucidating the detailed regulatory characteristics that can be attributed to the rnc gene. Global gene expression analysis revealed that the Δrnc strain exhibited 410 upregulated and 301 downregulated genes (fold-change > 1.5 and p < 0.05), as compared to the wild-type strain. Subsequent bioinformatics analysis indicated that these differentially expressed genes are involved in various physiological functions, in both the wild-type and Δrnc strains. This study provides evidence for the critical role of RNase III as a general positive regulator of flagellar-associated genes and its involvement in the pathogenicity of S. Typhimurium.

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CspA regulates stress resistance, flagellar motility and biofilm formation in Salmonella Enteritidis
Xiang Li, Yan Cui, Xiaohui Sun, Chunlei Shi, Shoukui He, Xianming Shi
Food Bioscience.2025; 66: 106237. CrossRef
The dual functions of the GTPase BipA in ribosome assembly and surface structure biogenesis in Salmonella enterica serovar Typhimurium
Eunsil Choi, Eunwoo Ryu, Donghwee Kim, Ji-Won Byun, Kahyun Kim, Minho Lee, Jihwan Hwang, Samuel Wagner
PLOS Pathogens.2025; 21(4): e1013047. CrossRef
Influence of Flagella on Salmonella Enteritidis Sedimentation, Biofilm Formation, Disinfectant Resistance, and Interspecies Interactions
Huixue Hu, Jingguo Xu, Jingyu Chen, Chao Tang, Tianhao Zhou, Jun Wang, Zhuangli Kang
Foodborne Pathogens and Disease.2024;[Epub] CrossRef

Azohydromonas aeria sp. nov., isolated from air: Han Xue , Chun-gen Piao , Dan-ran Bian , Min-wei Guo , Yong Li; J. Microbiol. 2020;58(7):543-549. Published online June 27, 2020; DOI: https://doi.org/10.1007/s12275-020-9423-x

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A grey pink colored bacterium, strain t3-1-3T, was isolated from the air at the foot of the Xiangshan Mountain in Beijing, China. The cells are aerobic, Gram-stain-negative, non-sporeforming, motile and coccoid-rod shaped (0.9–1.2 × 1.9–2.1 μm). Strain t3-1-3T was catalase-positive and oxidase-negative and this strain grew at 4–42°C (optimum 28°C), a pH of 4.0–9.0 (optimum pH 7.0) and under 0–2% (w/v) NaCl (optimum 0–1% NaCl). A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain t3-1-3T was closely related to Azohydromonas riparia UCM-11T (97.4% similarity), followed by Azohydromonas australica G1-2T (96.8%) and Azohydromonas ureilytica UCM-80T (96.7%). The genome of strain t3-1-3T contains 6,895 predicted protein-encoding genes, 8 rRNA genes, 62 tRNA genes and one sRNA gene, as well as five potential biosynthetic gene clusters, including clusters of genes coding for non-ribosomal peptide synthetase (NRPS), bacteriocin and arylpolyene and two clusters of genes for terpene. The predominant cellular fatty acids (> 10.0% of the total) in strain t3-1-3T were summed feature 3 (C16:1ω7c and/or C16:1ω6c, 37.8%), summed feature 8 (C18:1ω7c and/or C18:1ω6c, 29.7%) and C16:0 (17.3%). Strain t3-1-3T contained ubiquinone-8 (Q-8) as the predominant respiratory quinone. The polar lipids of strain t3-1-3T comprised phosphatidyl ethanolamine (PE), phosphatidyl glycerol (PG), diphosphatidyl glycerol (DPG), an unidentified glycolipid (GL), an unidentified aminophospholipid (APL), two unidentified phospholipid (PL1-2) and five unidentified lipid (L1-5). The DNA G + C content of the type strain is 70.3%. The broader range of growth temperature, assimilation of malic acid and trisodium citrate, presence of C18:3ω6c and an unidentified glycolipid and absence of C12:0 2-OH and C16:0iso differentiate strain t3-1-3T from related species. Based on the taxonomic data presented in this study, we suggest that strain t3-1-3T represents a novel species within the genus Azohydromonas, for which the name Azohydromonas aeria sp. nov. is proposed. The type strain of Azohydromonas aeria is t3-1-3T (= CFCC 13393T = LMG 30135T).

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Acid Phosphatase-Driven Advancements in Peanut Growth and Microbial Community Dynamics in Phosphorus-Limited Soils
Qi Wu, Liyu Yang, Yinglong Chen, Haiyan Liang, Miao Liu, Dianxu Chen, Pu Shen
Journal of Soil Science and Plant Nutrition.2025; 25(1): 83. CrossRef
The phylogeny of the genus Azohydromonas supports its transfer to the family Comamonadaceae
Ezequiel Gerardo Mogro, Juan Hilario Cafiero, Mauricio Javier Lozano, Walter Omar Draghi
International Journal of Systematic and Evolutionary Microbiology.2022;[Epub] CrossRef
In situ injectable nano-complexed hydrogel based on chitosan/dextran for combining tumor therapy via hypoxia alleviation and TAMs polarity regulation
Wenxue Zhang, Yan Shi, Hu Li, Miao Yu, Jiaxuan Zhao, Hao Chen, Ming Kong
Carbohydrate Polymers.2022; 288: 119418. CrossRef
Transformation of N and S pollutants and characterization of microbial communities in constructed wetlands with Vallisneria natans
Feichao Fu, Shaobin Huang, Heping Hu, Yao Lu, Yanlin Wang, Jianqi Yuan, Zerui Gong, Jinhua Wu, Yongqing Zhang
Journal of Water Process Engineering.2021; 42: 102186. CrossRef
Azohydromonas caseinilytica sp. nov., a Nitrogen-Fixing Bacterium Isolated From Forest Soil by Using Optimized Culture Method
Ram Hari Dahal, Dhiraj Kumar Chaudhary, Dong-Uk Kim, Jaisoo Kim
Frontiers in Microbiology.2021;[Epub] CrossRef

Research Support, Non-U.S. Gov't

Immunological Responses Induced by asd and wzy/asd Mutant Strains of Salmonella enterica serovar Typhimurium in BALB/c Mice: Hong Hua Piao , Vo Thi Minh Tam , Hee Sam Na , Hyun Ju Kim , Phil Youl Ryu , Soo Young Kim , Joon Haeng Rhee , Hyon E. Choy , Suhng Wook Kim , Yeongjin Hong; J. Microbiol. 2010;48(4):486-495. Published online August 20, 2010; DOI: https://doi.org/10.1007/s12275-010-0023-z

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Attenuated bacteria have long been developed as vaccine candidates but can have some disadvantages, such as the potential for damage to immune organs due to insufficient clearance. To minimize these disadvantages, we generated Salmonella enterica serovar Typhimurium mutants SHJ2104 (asd::cm) and HTSaYA (wzy::km, asd::cm). The wzy gene codes for the O-antigen polymerase, which is involved in lipopolysaccharide (LPS) biosynthesis, and asd codes for aspartate ß- semialdehyde dehydrogenase, which participates in cell wall formation. The strains synthesized LPS with a short-chain length, and showed lower cytotoxicity and reduced intracellular proliferation in animal cells compared to wild-type bacteria. After oral infection, the mutants were cleared in immune tissues, including the Peyer’s patch, mesenteric lymph node, and spleen, within 5 days. The LD50 of the mutants in Balb/c mice was estimated to be 106 higher than wild-type bacteria when administered either via an oral or i.p. route, indicating that the two strains are highly attenuated. To compare the immune response to and protective effects of the mutants against wild-type bacterial infection, we inoculated the mutants into mice via an oral (1×1010 CFU) or i.p. (1×107 CFU) route once or twice at a two week interval. All immune responses, such as serum IgG and secretory IgA levels, cytokine production, and delayed hypersensitivity, were highly induced by two rounds of immunization. HTSaYA and SHJ2104 induced similar immune responses, and mice immunized with HTSaYA or SHJ2104 via an i.p. route were protected against wild-type Salmonella infection even at 100-fold of the LD50 (5×106 CFU). Taken together, these data indicate that HTSaYA and SHJ2104 could be developed as live attenuated Salmonella vaccine candidates.

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The Evolution of Vaccines Development across Salmonella Serovars among Animal Hosts: A Systematic Review
Abubakar Siddique, Zining Wang, Haiyang Zhou, Linlin Huang, Chenghao Jia, Baikui Wang, Abdelaziz Ed-Dra, Lin Teng, Yan Li, Min Yue
Vaccines.2024; 12(9): 1067. CrossRef
Gut Microbiota Analysis and In Silico Biomarker Detection of Children with Autism Spectrum Disorder across Cohorts
Wenjuan Wang, Pengcheng Fu
Microorganisms.2023; 11(2): 291. CrossRef
Identification of small RNAs associated with RNA chaperone Hfq reveals a new stress response regulator in Actinobacillus pleuropneumoniae
Giarlã Cunha da Silva, Ciro César Rossi, Jéssica Nogueira Rosa, Newton Moreno Sanches, Daniela Lopes Cardoso, Yanwen Li, Adam A. Witney, Kate A. Gould, Patrícia Pereira Fontes, Anastasia J. Callaghan, Janine Thérèse Bossé, Paul Richard Langford, Denise Ma
Frontiers in Microbiology.2022;[Epub] CrossRef
VaximmutorDB: A Web-Based Vaccine Immune Factor Database and Its Application for Understanding Vaccine-Induced Immune Mechanisms
Kimberly Berke, Peter Sun, Edison Ong, Nasim Sanati, Anthony Huffman, Timothy Brunson, Fred Loney, Joseph Ostrow, Rebecca Racz, Bin Zhao, Zuoshuang Xiang, Anna Maria Masci, Jie Zheng, Guanming Wu, Yongqun He
Frontiers in Immunology.2021;[Epub] CrossRef
Efficacy of orally administered ginseng stem and leaf in chickens
Soyeon Park, Kwang-Yeal Lee, Youngjae Cho, Bokyoung Park, Kiju Kim, Na-Rae Lee, Dong-Gun Kim, Young-Hee Kim, Tae-Wook Hahn
Korean Journal of Veterinary Research.2015; 55(1): 1. CrossRef
Live attenuated vaccines for invasive Salmonella infections
Sharon M. Tennant, Myron M. Levine
Vaccine.2015; 33: C36. CrossRef
Attenuating gene expression (AGE) for vaccine development
David W Pascual, Zhiyong Suo, Ling Cao, Recep Avci, Xinghong Yang
Virulence.2013; 4(5): 384. CrossRef
Comparative evaluation of the murine immune responses to Salmonella enterica serovars Enteritidis, Gallinarum and Typhimurium infection
Kiju Kim, Dooree Kim, Jisun Sun, Soyeon Park, Youngjae Cho, Hyun-Jeong Ko, Hong-Gu Joo, Tae-Wook Hahn
Korean Journal of Veterinary Research.2013; 53(2): 95. CrossRef
STM2209-STM2208 (opvAB): A Phase Variation Locus of Salmonella enterica Involved in Control of O-Antigen Chain Length
Ignacio Cota, Anne Béatrice Blanc-Potard, Josep Casadesús, Dipshikha Chakravortty
PLoS ONE.2012; 7(5): e36863. CrossRef
Sublingual vaccination with sonicated Salmonella proteins and mucosal adjuvant induces mucosal and systemic immunity and protects mice from lethal enteritis
CHING-FENG HUANG, TZEE-CHUNG WU, CHIA-CHAO WU, CHIN-CHENG LEE, WEN-TSUNG LO, KWEI-SHUAI HWANG, MU-LING HSU, HO-JEN PENG
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