Journal of Microbiology

Full article

Genetic insights into novel lysis suppression by phage CSP1 in Escherichia coli: Moosung Kim, Sangryeol Ryu; J. Microbiol. 2025;63(4):e2501013. Published online April 29, 2025; DOI: https://doi.org/10.71150/jm.2501013

1,140 View
45 Download
1 Web of Science
1 Crossref

Lysis inhibition (LIN) in bacteriophage is a strategy to maximize progeny production. A clear plaque-forming mutant, CSP1C, was isolated from the turbid plaque-forming CSP1 phage. CSP1C exhibited an adsorption rate and replication dynamics similar to CSP1. Approximately 90% of the phages were adsorbed to the host cell within 12 min, and both phages had a latent period of 25 min. Burst sizes were 171.42 ± 31.75 plaque-forming units (PFU) per infected cell for CSP1 and 168.94 ± 51.67 PFU per infected cell for CSP1C. Both phages caused comparable reductions in viable E. coli cell counts at a low multiplicity of infection (MOI). However, CSP1 infection did not reduce turbidity, suggesting a form of LIN distinct from the well-characterized LIN of T4 phage. Genomic analysis revealed that a 4,672-base pairs (bp) DNA region, encompassing part of the tail fiber gene, CSP1_020, along with three hypothetical genes, CSP1_021, CSP1_022, and part of CSP1_023, was deleted from CSP1 to make CSP1C. Complementation analysis in CSP1C identified CSP1_020, CSP1_021, and CSP1_022 as a minimal gene set required for the lysis suppression in CSP1. Co-expression of these genes in E. coli with holin (CSP1_092) and endolysin (CSP1_091) resulted in lysis suppression. Lysis suppression was abolished by disrupting the proton motive force (PMF), supporting their potential role as antiholin. Additionally, CSP1_021 directly interacts with holin, suggesting that it may function as an antiholin. These findings identify new genetic factors involved in lysis suppression in CSP1, providing broader insights into phage strategies for modulating host cell lysis.

Citations

Citations to this article as recorded by

Characterization and genome analyses of the novel phages targeting extraintestinal Escherichia coli clones ST131 and ST410
Md Shamsuzzaman, Yoon-Jung Choi, Shukho Kim, Jungmin Kim
International Microbiology.2025;[Epub] CrossRef

Research Support, Non-U.S. Gov'ts

Simultaneous Detection of Waterborne Viruses by Multiplex Real-Time PCR: Lae-Hyung Kang , Se-hwan Oh , Jeong-Woong Park , Yu-Jung Won , Sangryeol Ryu , Soon-Young Paik; J. Microbiol. 2013;51(5):671-675. Published online September 14, 2013; DOI: https://doi.org/10.1007/s12275-013-3199-1

175 View
0 Download
6 Crossref

Abstract

Norovirus, Rotavirus group A, the Hepatitis A virus, and Coxsackievirus are all common causes of gastroenteritis. Conventional diagnoses of these causative agents are based on antigen detection and electron microscopy. To improve the diagnostic potential for viral gastroenteritis, internally controlled multiplex real-time polymerase chain reaction (PCR) methods have been recently developed. In this study, individual real-time PCRs were developed and optimized for specific detections of Norovirus genogroup I, Norovirus genogroup II, Rotavirus group A, the Hepatitis A virus, and Coxsackievirus group B1. Subsequently, individual PCRs were combined with multiplex PCR reactions. In general, multiplex real-time PCR assays showed comparable sensitivities and specificities with individual assays. A retrospective clinical evaluation showed increased pathogen detection in 29% of samples using conventional PCR methods. Prospective clinical evaluations were detected in 123 of the 227 (54%) total samples used in the multiplex realtime PCR analysis. The Norovirus genogroup II was found most frequently (23%), followed by Rotavirus (20%), the Hepatitis A virus (4.5%), Coxsackievirus (3.5%), and Norovirus genogroup I (2.6%). Internally controlled multiplex real-time PCR assays for the simultaneous detection of Rotavirus, Coxsackievirus group B, the Hepatitis A virus, and Norovirus genogroups I and II showed significant improvement in the diagnosis of viral gastroenteritis.

Citations

Citations to this article as recorded by

Microbiological quality of irrigation water for cultivation of fruits and vegetables: An overview of available guidelines, water testing strategies and some factors that influence compliance.
Oluwadara Alegbeleye, Anderson S. Sant’Ana
Environmental Research.2023; 220: 114771. CrossRef
Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality
Lisa Paruch
International Journal of Environmental Research and Public Health.2022; 19(9): 5128. CrossRef
Development and Evaluation of a SYBR Green-Based, Real-time Polymerase Chain Reaction for Rapid and Specific Detection of Human Coxsackievirus B5
Kyu Bong Cho
Biomedical Science Letters.2020; 26(4): 302. CrossRef
Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review
Mohammad Irshad, Priyanka Gupta, Dhananjay Singh Mankotia, Mohammad Ahmad Ansari
World Journal of Gastroenterology.2016; 22(20): 4824. CrossRef
Quantification and Genotyping of Rotavirus A within Two Wastewater Treatment Processes
Chourouk Ibrahim, Nadia Cherif, Salah Hammami, Pierre Pothier, Abdennaceur Hassen
CLEAN – Soil, Air, Water.2016; 44(4): 393. CrossRef
Microfluidic Quantitative PCR for Simultaneous Quantification of Multiple Viruses in Environmental Water Samples
Satoshi Ishii, Gaku Kitamura, Takahiro Segawa, Ayano Kobayashi, Takayuki Miura, Daisuke Sano, Satoshi Okabe, C. A. Elkins
Applied and Environmental Microbiology.2014; 80(24): 7505. CrossRef

ppGpp-Mediated Stationary Phase Induction of the Genes Encoded by Horizontally Acquired Pathogenicity Islands and cob/pdu Locus in Salmonella enterica serovar Typhimurium: Miryoung Song , Hyun-Ju Kim , Sangryeol Ryu , Hyunjin Yoon , Jiae Yun , Hyon E. Choy; J. Microbiol. 2010;48(1):89-95. Published online March 11, 2010; DOI: https://doi.org/10.1007/s12275-009-0179-6

173 View
0 Download
11 Crossref

Abstract

Salmonella enterica is highly diverse in terms of genome structure, which is at least partly due to the horizontal transfer of genetic elements from various sources. In this study, we examined the expression profiles of such genes in Salmonella Pathogenicity Islands (SPIs) and the cob/pdu locus, horizontally acquired large DNA segments, during growth under standard growth conditions. Transcripts from exponentially growing and early stationary phase Salmonellae were compared using various methods including cDNA microarray analysis. Nearly all genes encoded by SPIs and the cob/pdu locus were induced at the onset of the stationary phase in a stringent molecule ppGpp-dependent but stationary phase σ, σ38-independent manner. Although, it has been suggested that ppGpp acts in concert with DksA, we found the stationary phase induction of those SPI genes was not DksA dependent. It is suggested that ppGpp stimulates the expression of these stress-inducible genes encoded by horizontally acquired DNA, by itself or in concert with DksA.

Citations

Citations to this article as recorded by

Intrinsic antimicrobial resistance: Molecular biomaterials to combat microbial biofilms and bacterial persisters
Swagatam Barman, Leman Buzoglu Kurnaz, Ryan Leighton, Md Waliullah Hossain, Alan W. Decho, Chuanbing Tang
Biomaterials.2024; 311: 122690. CrossRef
Innovative Approaches of Engineering Tumor-Targeting Bacteria with Different Therapeutic Payloads to Fight Cancer: A Smart Strategy of Disease Management
Khaled S Allemailem
International Journal of Nanomedicine.2021; Volume 16: 8159. CrossRef
Use of Salmonella Bacteria in Cancer Therapy: Direct, Drug Delivery and Combination Approaches
Fereshteh Badie, Maryam Ghandali, Seyed Alireza Tabatabaei, Mahmood Safari, Ahmad Khorshidi, Mohammad Shayestehpour, Maryam Mahjoubin-Tehran, Korosh Morshedi, Amin Jalili, Vida Tajiknia, Michael R. Hamblin, Hamed Mirzaei
Frontiers in Oncology.2021;[Epub] CrossRef
Guanosine tetraphosphate relieves the negative regulation of Salmonella pathogenicity island-2 gene transcription exerted by the AT-rich ssrA discriminator region
Timothy Tapscott, Ju-Sim Kim, Matthew A. Crawford, Liam Fitzsimmons, Lin Liu, Jessica Jones-Carson, Andrés Vázquez-Torres
Scientific Reports.2018;[Epub] CrossRef
Mechanisms of Bacterial Tolerance and Persistence in the Gastrointestinal and Respiratory Environments
R. Trastoy, T. Manso, L. Fernández-García, L. Blasco, A. Ambroa, M. L. Pérez del Molino, G. Bou, R. García-Contreras, T. K. Wood, M. Tomás
Clinical Microbiology Reviews.2018;[Epub] CrossRef
Cell mass-dependent expression of an anticancer protein drug by tumor-targeted Salmonella
Kwangsoo Kim, Sa-Young Min, Ho-Dong Lim, Sung-Hwan You, Daejin Lim, Jae-Ho Jeong, Hyun-Ju Kim, Joon Haeng Rhee, Kyeongil Park, Minsang Shin, Geun-Joong Kim, Jung-Joon Min, Hyon E. Choy
Oncotarget.2018; 9(9): 8548. CrossRef
Anti-tumor activity of an immunotoxin (TGFα-PE38) delivered by attenuated Salmonella typhimurium
Daejin Lim, Kwang Soo Kim, Hyun-Ju Kim, Kyong-Cheol Ko, Jae Jun Song, Jong Hyun Choi, Minsang Shin, Jung-Joon Min, Jae-Ho Jeong, Hyon E. Choy
Oncotarget.2017; 8(23): 37550. CrossRef
Transcriptional and Post-Transcriptional Modulation of SPI1 and SPI2 Expression by ppGpp, RpoS and DksA in Salmonella enterica sv Typhimurium
Christopher J. Rice, Vinoy K. Ramachandran, Neil Shearer, Arthur Thompson, Dipshikha Chakravortty
PLOS ONE.2015; 10(6): e0127523. CrossRef
The Primary Transcriptome of Salmonella enterica Serovar Typhimurium and Its Dependence on ppGpp during Late Stationary Phase
Vinoy K. Ramachandran, Neil Shearer, Arthur Thompson, Muna Anjum
PLoS ONE.2014; 9(3): e92690. CrossRef
A Novel Balanced-Lethal Host-Vector System Based on glmS
Kwangsoo Kim, Jae Ho Jeong, Daejin Lim, Yeongjin Hong, Misun Yun, Jung-Joon Min, Sahng-June Kwak, Hyon E. Choy, Hiroshi Shiku
PLoS ONE.2013; 8(3): e60511. CrossRef
Engineering and Visualization of Bacteria for Targeting Infarcted Myocardium
Uyenchi N Le, Hyung-Seok Kim, Jin-Sook Kwon, Mi Yeon Kim, Vu H Nguyen, Sheng Nan Jiang, Byeong-Il Lee, Yeongjin Hong, Myung Geun Shin, Joon Haeng Rhee, Hee-Seung Bom, Youngkeun Ahn, Sanjiv S Gambhir, Hyon E Choy, Jung-Joon Min
Molecular Therapy.2011; 19(5): 951. CrossRef

First
Prev
Page of 1
Next
Last

Search