Journal of Microbiology

Isolation and Identification of an Anaerobic Dissimilatory Fe(III)-Reducing Bacterium, Shewanella putrefaciens IR-1: Moon-Sik Hyun , Byung-Hong Kim , In-Seop Chang , Hyung-Soo Park , Hyung-Joo Kim , Gwang-Tae Kim , Mi-a Kim , Doo-Hyun Park; J. Microbiol. 1999;37(4):206-212.

Abstract: In order to isolate a Fe(III)-reducer from the natural environment, soil samples were collected from various patty fields and enriched with ferric citrate as a source of Fe(III) under anaerobic condition. Since the enrichment culture was serially performed, the Fe(III)-reduction activity was serially diluted and cultivated on an agar plate containing lactate and ferric citrate in an anaerobic glove box. A Gram negative, motile, rod-shpaped and facultative anaerobic Fe(III)-reducer was isolated based on its highest Fe(III)-reduction activity, Bacterial growth was coupled with oxidation of lactate to Fe(III)-reduction, but the isolate fermented pyruvate without Fe(III), The isolate reduced an insoluble ferric iron (FeOOH) as well as a soluble ferric iron (ferric citrate). Using the BBL crystal enteric/non-fermentor identification kit and 16S rDNA sequence analysis, the isolate was identifice as Shewanella putrefaciens IR-1.

Growth Properties of the Iron-reducing Bacteria, Shewanella putrefaciens IR-1 and MR-1 Coupling to Reduction of Fe(III) to Fe(II): Doo Hyun Park , Byung Hong Kim; J. Microbiol. 2001;39(4):273-278.

Abstract: Shewanella putrefaciens IR-1 and MR-1 were cultivated by using various combinations of electron donor-acceptor, lactate-Fe(III), lactate-nitrate, pyruvate-Fe(III), pyruvate-nitrate, H_2 -acetate-Fe(III) and H_2 -acetate-nitrate. Both strains grew fermentatively on pyruvate but not on lactate without an electron acceptor. In culture with Fe(III), both strains grew on pyruvate and lactate but not on H_2 -acetate-CO_2 . In cultivation with nitrate, both strains grew on pyruvate, lactate and on H_2 -acetate-CO_2 . The growth yields of IR-1 on pyruvate, pyruvate-Fe(III) and lactate-Fe(III) were about 3.4, 3.5, and 3.6 (g cell/M substrate), respectively, but the yields on lactate-nitrate, pyruvate-nitrate and H_2 -acetate-CO_2 ?trate were about 6.8, 5.9, and 9.4 (g cell/M substrate), respectively. From the growth properties of both strains on media with Fe(III) as an electron acceptor, the bacterial growth was confirmed not to be increased by addition of Fe(III) as an electron acceptor to the growth medium, which indicates a possibility that the dissimilatory reduction of Fe(III) to Fe(II) may not be coupled to free energy production.

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