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- Surface Display of the HPV L1 Capsid Protein by the Autotransporter Shigella IcsA
- Dan Xu , Xiaofeng Yang , Depu Wang , Jun Yu , Yili Wang
- J. Microbiol. 2014;52(1):77-82. Published online January 4, 2014
- DOI: https://doi.org/10.1007/s12275-014-3235-9
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- 3 Crossref
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Abstract
- Autotransporters have become attractive tools for surface expression of foreign proteins in Gram-negative bacteria. In this study, the Shigella autotransporter IcsA, has been exploited to express the human papillomavirus (HPV) type 16 L1 capsid protein in Shigella sonnei and Escherichia coli. The L1 gene was fused in-frame to replace the coding sequence of the IcsA passenger domain that is responsible for actin-based motility. The resultant hybrid protein could be detected by an anti-L1 antibody on the surface of S. sonnei and E. coli. In E. coli, the protein was expressed on the entire surface of the bacterium. In contrast, the protein was detected mainly at one pole of the Shigella bacterium. However, the protein became evenly distributed on the surface of the Shigella bacterium when the icsP gene was removed. Our study demonstrated the possibility of exploiting autotransporters for surface expression of large, heterologous viral proteins, which may be a useful strategy for vaccine development.
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Citations
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- The Trimeric Autotransporter Adhesin SadA from Salmonella spp. as a Novel Bacterial Surface Display System
Shuli Sang, Wenge Song, Lu Lu, Qikun Ou, Yiyan Guan, Haoxia Tao, Yanchun Wang, Chunjie Liu
Vaccines.2024; 12(4): 399. CrossRef - The Shigella ProU system is required for osmotic tolerance and virulence
Rasha Y. Mahmoud, Wenqin Li, Ramadan A. Eldomany, Mohamed Emara, Jun Yu
Virulence.2017; 8(4): 362. CrossRef - The Multivalent Adhesion Molecule SSO1327 plays a key role in Shigella sonnei pathogenesis
Rasha Y. Mahmoud, Daniel Henry Stones, Wenqin Li, Mohamed Emara, Ramadan A. El‐domany, Depu Wang, Yili Wang, Anne Marie Krachler, Jun Yu
Molecular Microbiology.2016; 99(4): 658. CrossRef
- The Trimeric Autotransporter Adhesin SadA from Salmonella spp. as a Novel Bacterial Surface Display System
- Comparative Genomic Analysis of Bacteriophage EP23 Infecting Shigella sonnei and Escherichia coli
- Ho-Won Chang , Kyoung-Ho Kim
- J. Microbiol. 2011;49(6):927-934. Published online December 28, 2011
- DOI: https://doi.org/10.1007/s12275-011-1577-0
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- 19 Scopus
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Abstract
- Bacteriophage EP23 that infects Escherichia coli and Shigella sonnei was isolated and characterized. The bacteriophage morphology was similar to members of the family Siphoviridae. The 44,077 bp genome was fully sequenced using 454 pyrosequencing. Comparative genomic and phylogenetic analyses showed that EP23 was most closely related to phage SO-1, which infects Sodalis glossinidius and phage SSL-2009a, which infects engineered E. coli. Genomic comparison indicated that EP23 and SO-1 were very similar with each other in terms of gene order and amino acid similarity, even though their hosts were separated in the level of genus. EP23 and SSL-2009a displayed high amino acid similarity between their genes, but there was evidence of several recombination events in SSL-2009a. The results of the comparative genomic analyses further the understanding of the evolution and relationship between EP23 and its bacteriophage relatives.
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