Journal of Microbiology

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Genotyping, Morphology and Molecular Characteristics of a Lytic Phage of Neisseria Strain Obtained from Infected Human Dental Plaque: Ahmed N Aljarbou , Mohamad Aljofan; J. Microbiol. 2014;52(7):609-618. Published online May 30, 2014; DOI: https://doi.org/10.1007/s12275-014-3380-1

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The lytic bacteriaphage (phage) A2 was isolated from human dental plaques along with its bacterial host. The virus was found to have an icosahedron-shaped head (60±3 nm), a sheathed and rigid long tail (~175 nm) and was categorized into the family Siphoviridae of the order Caudovirales, which are dsDNA viral family, characterised by their ability to infect bacteria and are nonenveloped with a noncontractile tail. The isolated phage contained a linear dsDNA genome having 31,703 base pairs of unique sequence, which were sorted into three contigs and 12 single sequences. A latent period of 25 minutes and burst size of 24±2 particles was determined for the virus. Bioinformatics approaches were used to identify ORFs in the genome. A phylogenetic analysis confirmed the species inter-relationship and its placement in the family.

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Periodontitis: etiology, conventional treatments, and emerging bacteriophage and predatory bacteria therapies
Anna Łasica, Piotr Golec, Agnieszka Laskus, Magdalena Zalewska, Magdalena Gędaj, Magdalena Popowska
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Screening of Anorectal and Oropharyngeal Samples Fails to Detect Bacteriophages Infecting Neisseria gonorrhoeae
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Jonathon L. Baker, Batbileg Bor, Melissa Agnello, Wenyuan Shi, Xuesong He
Trends in Microbiology.2017; 25(5): 362. CrossRef
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Superinfection Exclusion Reveals Heteroimmunity between Pseudomonas aeruginosa Temperate Phages: In-Young Chung , Hee-Won Bae , Hye-Jung Jang , Bi-o Kim , You-Hee Cho; J. Microbiol. 2014;52(6):515-520. Published online May 29, 2014; DOI: https://doi.org/10.1007/s12275-014-4012-5

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Abstract

Temperate siphophages (MP29, MP42, and MP48) were isolated from the culture supernatant of clinical Pseudomonas aeruginosa isolates. The complete nucleotide sequences and annotation of the phage genomes revealed the overall synteny to the known temperate P. aeruginosa phages such as MP22, D3112, and DMS3. Genome-level sequence analysis showed the conservation of both ends of the linear genome and the divergence at the previously identified dissimilarity regions (R1 to R9). Protein sequence alignment of the c repressor (ORF1) of each phage enabled us to divide the six phages into two groups: D3112 group (D3112, MP29, MP42, and MP48) and MP22 group (MP22 and DMS3). Superinfection exclusion was observed between the phages belonging to the same group, which was mediated by the specific interaction between the c repressor and the cognate operator. Based on these, we suggest that the temperate siphophages prevalent in the clinical strains of P. aeruginosa represent at least two distinct heteroimmunity groups.

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A phage protein that inhibits the bacterial ATPase required for type IV pilus assembly
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Comparative Genomic Analysis of Bacteriophage EP23 Infecting Shigella sonnei and Escherichia coli: Ho-Won Chang , Kyoung-Ho Kim; J. Microbiol. 2011;49(6):927-934. Published online December 28, 2011; DOI: https://doi.org/10.1007/s12275-011-1577-0

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Abstract: Bacteriophage EP23 that infects Escherichia coli and Shigella sonnei was isolated and characterized. The bacteriophage morphology was similar to members of the family Siphoviridae. The 44,077 bp genome was fully sequenced using 454 pyrosequencing. Comparative genomic and phylogenetic analyses showed that EP23 was most closely related to phage SO-1, which infects Sodalis glossinidius and phage SSL-2009a, which infects engineered E. coli. Genomic comparison indicated that EP23 and SO-1 were very similar with each other in terms of gene order and amino acid similarity, even though their hosts were separated in the level of genus. EP23 and SSL-2009a displayed high amino acid similarity between their genes, but there was evidence of several recombination events in SSL-2009a. The results of the comparative genomic analyses further the understanding of the evolution and relationship between EP23 and its bacteriophage relatives.

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