Journal of Microbiology

Research Support, Non-U.S. Gov't

Molecular and Phylogenetic Characterization of Spodoptera litura Granulovirus: Yong Wang , Jae Young Choi , Jong Yul Roh , Soo Dong Woo , Byung Rae Jin , Yeon Ho Je; J. Microbiol. 2008;46(6):704-708. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0133-z

Abstract: Baculovirus, Spodoptera litura granulovirus (SlGV) was isolated from the infected S. litura larvae, and was characterized. The granule of SlGV was ovoidal shape with an approximate size of 240~340 nm×140~180 nm. Each granule contained one single rod-shape virion with a mean size of 180~200 nm×20~40 nm. Restriction endonuclease fragment analysis estimated that the total genome size of SlGV is about 115 kb. Necleotide sequence analysis of the granulin gene showed that the gene encodes 249 amino acids with a predicted molecular mass of 29 kDa. When the phylogenic relationship was analyzed using the nucleotide sequence of the granulin gene, SlGV was most closely related to Trichoplusia ni granulovirus (TnGV) and Xestia c-nigrum granulovirus (XcGV) which belong to Type I granulovirus.

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