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- Identification and characterization of a new agar-degrading strain with the novel properties of saccharides inhibition and nitrogen fixation
- Hao Wu , Guiguang Chen , Yaxi Bian , Wei Zeng , Bihong Sun , Zhiqun Liang
- J. Microbiol. 2017;55(6):475-482. Published online May 28, 2017
- DOI: https://doi.org/10.1007/s12275-017-6464-x
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Abstract
- In this study, a new agar-degrading strain was isolated from soil with agar as a sole carbon source and energy. Based on its morphological, physiological, biochemical characterization and 16S rDNA sequence, the strain was identified as Strep-tomyces lavendulae UN-8. The extracellular agarase activity reached 0.03 U/ml after fermentation in shake flask (250 ml), which was close to other reported non-marine micro-organisms. Furthermore, it is interesting that the growth of UN-8 would be inhibited by glucose (40 g/L) and maltose (40 g/L) with the inhibitory rate of 100% and 70%, respec-tively. Besides, UN-8 could be grown on the solid medium without any nitrogen sources, then the possible nitrogen fix-ation gene nifU was cloned from its genomic DNA. The de-duced amino acid sequence of nifU has high similarity (98%) with nitrogen fixation protein NifU from Streptomyces sp. NRRL S-104 (KJY22454.1) and Streptomyces sp. NRRL F-4428 (KJK52526.1) based on NCBI blast. It is suggested that the nifU gene of UN-8 also encoded nitrogen fixation protein NifU. These results provided some new information for the further understanding of agar-degrading strain.
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Citations
Citations to this article as recorded by
- A Novel Strategy to Regulate 1-Deoxynojirimycin Production Based on Its Biosynthetic Pathway in Streptomyces lavendulae
Hao Wu, Ye Guo, Lei Chen, Guiguang Chen, Zhiqun Liang
Frontiers in Microbiology.2019;[Epub] CrossRef
- A Novel Strategy to Regulate 1-Deoxynojirimycin Production Based on Its Biosynthetic Pathway in Streptomyces lavendulae
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