Journal of Microbiology

Research Support, Non-U.S. Gov'ts

Soil fungal communities of montane natural secondary forest types in China: Fei Cheng , Xin Wei , Lin Hou , Zhengchun Shang , Xiaobang Peng , Peng Zhao , Zhaoxue Fei , Shuoxin Zhang; J. Microbiol. 2015;53(6):379-389. Published online May 30, 2015; DOI: https://doi.org/10.1007/s12275-015-4722-3

48 View
0 Download
9 Crossref

Distinctive plant communities may provide specific physical and chemical properties with soils by specific litters and root exudates to exert effects on soil microorganisms. Past logging activities in the Qinling Mountains induced diverse natural secondary forest types (NSFTs). How these recovered NSFTs regulate patterns of soil microbial communities remain limited. In the study, we used terminal-restriction fragment length polymorphism (T-RFLP) to precisely determine forest type-specific soil fungal diversity and composition in five NSFTs. Our results indicated that NSFTs had significant impacts on the soil fungal communities. The most diverse fungal species were found in the Armand pine (Pinus armandi) and Chinese pine (Pinus tabulaeformis) forest soils, followed by sharptooth oak (Quercus aliena var. acuteserrata) and Chinese pine-sharptooth oak forest soils, the wilson spruce (Picea wilsonii) forests had the lowest soil fungal diversity. The analyses of community composition suggested that the fungal communities of Armand pine forest soils were similar to those of Chinese pine forest soils, while other communities prominently differed from each other. Stepwise multiple regression analysis revealed that soil silt, clay, pH, and ammonium nitrogen had intimate linkages with soil fungal diversity. Furthermore, the patterns of soil fungal communites were strongly governed by the specific soil environments of the tested NSFTs, as described by canonical correspondence analysis (CCA). Finally, our study showed that soil fungal communities may be mediated by NSFTs via specific soil edaphic status. Hence, such a comparable study may provide fundamental information for fungal diversity and community structure of natural forests and assist with better prediction and understanding how soil fungal composition and function alter with forest type transformation.

Citations

Citations to this article as recorded by

Soil Fungal Community Characteristics at Timberlines of Sejila Mountain in Southeast Tibet, China
Fei Cheng, Mingman Li, Yihua Ren, Lei Hou, Tan Gao, Peng He, Xiangsheng Deng, Jie Lu
Journal of Fungi.2023; 9(5): 596. CrossRef
Soil characteristics and microbial community structure on along elevation gradient in a Pinus armandii forest of the Qinling Mountains, China
Yonghua Zhao, Yujie Zhou, Xia Jia, Lei Han, Li Liu, Kun Ren, Xuan Ye, Zhi Qu, Yuanjie Pei
Forest Ecology and Management.2022; 503: 119793. CrossRef
Spatial characteristics of the dominant fungi and their driving factors in forest soils in the Qinling Mountains, China
Yujie Zhou, Xia Jia, Lei Han, Ge Tian, Shuaizhi Kang, Yonghua Zhao
CATENA.2021; 206: 105504. CrossRef
Short-Term Effects of Different Forest Management Methods on Soil Microbial Communities of a Natural Quercus aliena var. acuteserrata Forest in Xiaolongshan, China
Pan Wan, Gongqiao Zhang, Zhonghua Zhao, Yanbo Hu, Wenzhen Liu, Gangying Hui
Forests.2019; 10(2): 161. CrossRef
Influence of seasonality and management practices on diversity and composition of fungal communities in vineyard soils
Maria M. Hernandez, Cristina M. Menéndez
Applied Soil Ecology.2019; 135: 113. CrossRef
Seasonal dynamics of bacterial communities in aBetula albosinensisforest
C. Du, C.‐Y. Xu, J.‐S. Jian, W.‐X. He, L. Hou, Z.‐C. Geng
European Journal of Soil Science.2018; 69(4): 666. CrossRef
Rhododendron aureum Georgi formed a special soil microbial community and competed with above‐ground plants on the tundra of the Changbai Mountain, China
Xiaolong Wang, Lin Li, Wei Zhao, Jiaxin Zhao, Xia Chen
Ecology and Evolution.2017; 7(18): 7503. CrossRef
Variations in bacterial and fungal communities through soil depth profiles in a Betula albosinensis forest
Can Du, Zengchao Geng, Qiang Wang, Tongtong Zhang, Wenxiang He, Lin Hou, Yueling Wang
Journal of Microbiology.2017; 55(9): 684. CrossRef
A comparison of species composition and community assemblage of secondary forests between the birch and pine-oak belts in the mid-altitude zone of the Qinling Mountains, China
Zongzheng Chai, Dexiang Wang
PeerJ.2016; 4: e1900. CrossRef

The Role of a Dark Septate Endophytic Fungus, Veronaeopsis simplex Y34, in Fusarium Disease Suppression in Chinese Cabbage: Rida O. Khastini , Hiroyuki Ohta , Kazuhiko Narisawa; J. Microbiol. 2012;50(4):618-624. Published online August 25, 2012; DOI: https://doi.org/10.1007/s12275-012-2105-6

41 View
0 Download
35 Scopus

Abstract: The soil-inhabiting fungal pathogen Fusarium oxysporum has been an increasing threat to Chinese cabbage (Brassica campestris L.). A dark septate endophytic fungus, Veronaeopsis simplex Y34, isolated from Yaku Island, Japan, was evaluated in vitro for the ability to suppress Fusarium disease. Seedlings grown in the presence of the endophyte showed a 71% reduction in Fusarium wilt disease and still had good growth. The disease control was achieved through a synergetic effect involving a mechanical resistance created by a dense network of V. simplex Y34 hyphae, which colonized the host root, and siderophore production acting indirectly to induce a resistance mechanism in the plant. Changes in the relative abundance of the fungal communities in the soil as determined by fluorescently labelled T-RFs (terminal restriction fragments), appeared 3 weeks after application of the fungus. Results showed the dominance of V. simplex Y34, which became established in the rhizosphere and out-competed F. oxysporum.

Microbial Fingerprinting Detects Unique Bacterial Communities in the Faecal Microbiota of Rats with Experimentally-Induced Colitis: Ashis K. Samanta , Valeria A. Torok , Nigel J. Percy , Suzanne M. Abimosleh , Gordon S. Howarth; J. Microbiol. 2012;50(2):218-225. Published online April 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1362-8

36 View
0 Download
43 Scopus

Abstract: An abnormal composition of the gut microbiota is believed to be associated with the pathogenesis of inflammatory bowel disease (IBD). We utilized terminal restriction fragment length polymorphism (T-RFLP) analysis to quantify faecal bacterial communities from rats with experimental colitis. Male Sprague Dawley rats (n=10/group) ingested 2% dextran sulfate sodium (DSS) or water for up to 7 days. Rats were killed and colonic tissues collected for histological analysis. Damage severity score in the distal colon was significantly greater (P<0.001) following DSS consumption compared to controls. T-RFLP faecal bacterial profiles generated with either MspI or CfoI revealed a significant difference (P<0.001) in community composition between healthy and colitic rats, with bacterial composition in healthy rats more variable than in rats with colitis. Operational taxonomic units (OTU: taxonomically related groups of bacteria) associated with either the healthy or colitic state were identified. OTU (116, 226, 360, and 948; CfoI) and (118 and 188; MspI) were strongly associated with untreated healthy rats, while OTU (94, 98, 174, and 384; CfoI) and (94 and 914; MspI) were predominantly associated with DSS-treated colitic rats. Phylogenetic OTU assignment suggested that Bacteroidales and Lactobacillus sp. were predominantly associated with the colitic and healthy rats, respectively. These
results
show that faecal bacterial profiling is a rapid, sensitive and non-invasive tool for detecting and identifying changes in gut microbiota associated with colitis. Restoring microbial homeostasis by targeting colitis-associated OTU through specific microbiological interventions could form the basis of novel therapeutic strategies for IBD.

Journal Article

Molecular Identification of Fecal Pollution Sources in Water Supplies by Host-Specific Fecal DNA Markers and Terminal Restriction Fragment Length Polymorphism Profiles of 16S rRNA Gene: Ju-Yong Jeong , Kyung-Ik Gil , Kyong-Hee Lee , Jong-Ok Ka; J. Microbiol. 2008;46(6):599-607. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0174-3

37 View
0 Download
5 Scopus

Abstract: Specific fecal DNA markers were investigated for major pollution sources, cow, human, and pig, and occurrence of the identified markers was analyzed in river waters using Terminal Restriction Fragment Length Polymorphism (T-RFLP) techniques and sequencing of 16S rDNA of Bacteroides-Prevotella. The unique and specific DNA markers for cow and human were identified as a 222 bp and 60 bp peak in HaeIII T-RFLP profiles, respectively, and the pig-specific marker was not identified but the unique T-RFLP profile of pig could be used as a substitution. Human-specific marker was detected in most of the river waters tested (92.1%) and T-RFLP profiles of river waters were shown to be similar to those of human feces. Cluster analysis of T-RFLP data showed that the fecal sources were multiple (human plus cow and human plus dairy cow) in most of the river waters. The phylogenetic analysis for the clones recovered from the fecal and water samples showed that the clones from cow formed a discreet cluster from those of other sources. The other clones from human, pig, and river water formed two groups all together. The results of this study could be used to identify and control the fecal pollution source in the bodies of water in Korea.

Research Support, Non-U.S. Gov't

Bacterial Communities in the Initial Stage of Marine Biofilm Formation on Artificial Surfaces: Jin-Woo Lee , Ji-Hyun Nam , Yang-Hoon Kim , Kyu-Ho Lee , Dong-Hun Lee; J. Microbiol. 2008;46(2):174-182. Published online June 11, 2008; DOI: https://doi.org/10.1007/s12275-008-0032-3

36 View
0 Download
197 Scopus

Abstract: Succession of bacterial communities during the first 36 h of biofilm formation in coastal water was investigated at 3~15 h intervals. Three kinds of surfaces (i.e., acryl, glass, and steel substratum) were submerged in situ at Sacheon harbor, Korea. Biofilms were harvested by scraping the surfaces, and the compositions of bacterial communities were analyzed by terminal restriction fragment length polymorphism (T-RFLP), and cloning and sequencing of 16S rRNA genes. While community structure based on T-RFLP analysis showed slight differences by substratum, dramatic changes were commonly observed for all substrata between 9 and 24 h. Identification of major populations by 16S rRNA gene sequences indicated that γ-Proteobacteria (Pseudomonas, Acinetobacter, Alteromonas, and uncultured γ-Proteobacteria) were predominant in the community during 0~9 h, while the ratio of α-Proteobacteria (Loktanella, Methylobacterium, Pelagibacter, and uncultured α-Proteobacteria) increased 2.6~4.8 folds during 24~36 h of the biofilm formation, emerging as the most predominant group. Previously, α-Proteobacteria were recognized as the pioneering organisms in marine biofilm formation. However, results of this study, which revealed the bacterial succession with finer temporal resolution, indicated some species of γ-Proteobacteria were more important as the pioneering population. Measures to control pioneering activities of these species can be useful in prevention of marine biofilm formation.

First
Prev
Page of 1
Next
Last

Search