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- Characterization and Comparative Genomic Analysis of vB_BceM_CEP1: A Novel Temperate Bacteriophage Infecting Burkholderia cepacia Complex
- Momen Askoura, Eslam K Fahmy, Safya E Esmaeel, Wael A H Hegazy, Aliaa Abdelghafar
- J. Microbiol. 2024;62(11):1035-1055. Published online November 18, 2024
- DOI: https://doi.org/10.1007/s12275-024-00185-2
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Abstract
- The increasing prevalence of multidrug-resistant bacteria imminently threatens public health and jeopardizes nearly all aspects of modern medicine. The Burkholderia cepacia complex (Bcc) comprises Burkholderia cepacia and the related species of Gram-negative bacteria. Members of the Bcc group are opportunistic pathogens responsible for various chronic illnesses, including cystic fibrosis and chronic granulomatous disease. Phage therapy is emerging as a potential solution to combat the antimicrobial resistance crisis. In this study, a temperate phage vB_BceM_CEP1 was isolated from sewage and fully characterized. Transmission electron microscopy indicated that vB_BceM_CEP1 belongs to the family Peduoviridae. The isolated phage demonstrated enhanced environmental stability and antibiofilm potential. One-step growth analysis revealed a latent period of 30 min and an average burst size of 139 plaque-forming units per cell. The genome of vB_BceM_CEP1 consists of 32,486 bp with a GC content of 62.05%. A total of 40 open reading frames were annotated in the phage genome, and none of the predicted genes was annotated as tRNA. Notably, genes associated with antibiotic resistance, host virulence factors, and toxins were absent from the vB_BceM_CEP1 genome. Based on its unique phenotype and phylogeny, the isolated phage vB_BceM_CEP1 is classified as a new temperate phage with lytic activity. The findings of this study enhance our understanding of the diversity of Bcc phages.
Research Support, Non-U.S. Gov't
- Superinfection Exclusion Reveals Heteroimmunity between Pseudomonas aeruginosa Temperate Phages
- In-Young Chung , Hee-Won Bae , Hye-Jung Jang , Bi-o Kim , You-Hee Cho
- J. Microbiol. 2014;52(6):515-520. Published online May 29, 2014
- DOI: https://doi.org/10.1007/s12275-014-4012-5
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- 4 Crossref
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Abstract
- Temperate siphophages (MP29, MP42, and MP48) were isolated from the culture supernatant of clinical Pseudomonas aeruginosa isolates. The complete nucleotide sequences and annotation of the phage genomes revealed the overall synteny to the known temperate P. aeruginosa phages such as MP22, D3112, and DMS3. Genome-level sequence analysis showed the conservation of both ends of the linear genome and the divergence at the previously identified dissimilarity regions (R1 to R9). Protein sequence alignment of the c repressor (ORF1) of each phage enabled us to divide the six phages into two groups: D3112 group (D3112, MP29, MP42, and MP48) and MP22 group (MP22 and DMS3). Superinfection exclusion was observed between the phages belonging to the same group, which was mediated by the specific interaction between the c repressor and the cognate operator. Based on these, we suggest that the temperate siphophages prevalent in the clinical strains of P. aeruginosa represent at least two distinct heteroimmunity groups.
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Citations
Citations to this article as recorded by
- Phage against the Machine: The SIE-ence of Superinfection Exclusion
Michael J. Bucher, Daniel M. Czyż
Viruses.2024; 16(9): 1348. CrossRef - Transposition Behavior Revealed by High-Resolution Description of Pseudomonas Aeruginosa Saltovirus Integration Sites
Gilles Vergnaud, Cédric Midoux, Yann Blouin, Maria Bourkaltseva, Victor Krylov, Christine Pourcel
Viruses.2018; 10(5): 245. CrossRef - Evolutionary Ecology of Prokaryotic Immune Mechanisms
Stineke van Houte, Angus Buckling, Edze R. Westra
Microbiology and Molecular Biology Reviews.2016; 80(3): 745. CrossRef - A phage protein that inhibits the bacterial ATPase required for type IV pilus assembly
In-Young Chung, Hye-Jeong Jang, Hee-Won Bae, You-Hee Cho
Proceedings of the National Academy of Sciences.2014; 111(31): 11503. CrossRef
- Phage against the Machine: The SIE-ence of Superinfection Exclusion
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