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- The C-22 sterol desaturase Erg5 is responsible for ergosterol biosynthesis and conidiation in Aspergillus fumigatus
- Nanbiao Long , Guowei Zhong
- J. Microbiol. 2022;60(6):620-626. Published online April 18, 2022
- DOI: https://doi.org/10.1007/s12275-022-1564-7
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- 4 Crossref
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Abstract
- Aspergillus fumigatus is the most prevalent saprophytic fungi and can cause severe invasive aspergillosis in immunocompromised individuals. For infection of A. fumigatus, the small hydrophobic conidia have been shown to play a dominant role. In this study, we found that deletion of erg5, a C-22 sterol desaturase gene which function in the last two steps of ergosterol biosynthesis, was sufficient to block ergosterol biosynthesis and conidiation. The deletion phenotype was further verified by a conditional expression strain of erg5 using the inducible tet-on system. Strikingly, erg5 mutant displays increased susceptibility to antifungal azoles itraconazole. RNA sequencing analysis showed that erg5 deficiency resulted in changes in transcription mainly related to lipid, carbohydrate, and amino acid metabolism. Genes encoding ergosterol biosynthesis- related enzymes were found to be up-regulated in erg5 null mutants. However, genes involved in asexual development, including upstream regulators, melanin biosynthesis enzymes, heterotrimeric G proteins, and MAPK signaling, were down-regulated to various degrees. Furthermore, metabolomic study revealed that erg5 deficiency also resulted in altered lipid and amino acid metabolism, which was consistent with our transcriptomics analysis. Collectively, our study established a link between ergosterol biosynthesis and asexual development at the transcriptomics and metabolomics level in A. fumigatus.
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Citations
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- Quantitative proteomic analysis reveals Ga(III) polypyridyl catecholate complexes disrupt Aspergillus fumigatus mitochondrial function
Magdalena Piatek, Brunella Grassiri, Lewis More O’Ferrall, Anna Maria Piras, Giovanna Batoni, Semih Esin, Christine O’Connor, Darren Griffith, Anne Marie Healy, Kevin Kavanagh
JBIC Journal of Biological Inorganic Chemistry.2024; 29(7-8): 707. CrossRef - Ergosterol Is Critical for Sporogenesis in Cryptococcus neoformans
Amber R. Matha, Xiaofeng Xie, Xiaorong Lin
Journal of Fungi.2024; 10(2): 106. CrossRef - Erg4 Is Involved in Ergosterol Biosynthesis, Conidiation and Stress Response in Penicillium expansum
Zhanhong Han, Yuanyuan Zong, Xuemei Zhang, Di Gong, Bin Wang, Dov Prusky, Edward Sionov, Huali Xue, Yang Bi
Journal of Fungi.2023; 9(5): 568. CrossRef -
A chromosome-scale genome assembly of the grape powdery mildew pathogen
Erysiphe necator
reveals its genomic architecture and previously unknown features of its biology
Alex Z. Zaccaron, Tara Neill, Jacob Corcoran, Walter F. Mahaffee, Ioannis Stergiopoulos, Gustavo H. Goldman
mBio.2023;[Epub] CrossRef
- Quantitative proteomic analysis reveals Ga(III) polypyridyl catecholate complexes disrupt Aspergillus fumigatus mitochondrial function
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