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- Low Cell Density Regulator AphA Upregulates the Expression of Vibrio vulnificus iscR Gene Encoding the Fe-S Cluster Regulator IscR
- Jong Gyu Lim , Jin Hwan Park , Sang Ho Choi
- J. Microbiol. 2014;52(5):413-421. Published online February 17, 2014
- DOI: https://doi.org/10.1007/s12275-014-3592-4
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- 11 Crossref
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Abstract
- IscR is a global transcriptional regulator that contributes to the pathogenesis of Vibrio vulnificus, a food-borne pathogen. In the present study, the regulatory mechanism for the iscR expression of V. vulnificus was evaluated. The expression of iscR was found to be upregulated by a transcriptional regu-lator AphA, a homologue of the low cell density regulator AphA of the Vibrio species, in the exponential phase of growth. The promoter activity of iscR appeared to be acti-vated and repressed by AphA and IscR, respectively. EMSA and DNase I protection assay showed that both AphA and IscR bind to the iscR regulatory region and the binding site for AphA overlapped with part of the binding site for IscR. Further mutational analysis suggested that AphA upregu-lates the iscR expression only in the presence of functional IscR. An examination of the roles of AphA and the binding sites revealed that the binding of AphA would hinder the IscR-mediated repression of the iscR transcription. The com-bined results show that V. vulnificus AphA upregulates iscR expression by antagonizing its negative autoregulation.
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Citations
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- Environmental magnesium ion affects global gene expression, motility, biofilm formation and virulence of Vibrio parahaemolyticus
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Biofilm.2024; 7: 100194. CrossRef - Fe-S cluster homeostasis and beyond: The multifaceted roles of IscR
Erin L. Mettert, Patricia J. Kiley
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Vibrios
Renfei Lu, George Osei-Adjei, Xinxiang Huang, Yiquan Zhang
Future Microbiology.2018; 13(3): 383. CrossRef - Crystal Structure of the Regulatory Domain of AphB from Vibrio vulnificus, a Virulence Gene Regulator
Nohra Park, Saemee Song, Garam Choi, Kyung Ku Jang, Inseong Jo, Sang Ho Choi, Nam-Chul Ha
Molecules and Cells.2017; 40(4): 299. CrossRef - Iron- and Quorum-sensing Signals Converge on Small Quorum-regulatory RNAs for Coordinated Regulation of Virulence Factors in Vibrio vulnificus
Yancheng Wen, In Hwang Kim, Kun-Soo Kim
Journal of Biological Chemistry.2016; 291(27): 14213. CrossRef - What a difference a cluster makes: The multifaceted roles of IscR in gene regulation and DNA recognition
Joana A. Santos, Pedro José Barbosa Pereira, Sandra Macedo-Ribeiro
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics.2015; 1854(9): 1101. CrossRef - Characterization of the Vibrio vulnificus 1-Cys Peroxiredoxin Prx3 and Regulation of Its Expression by the Fe-S Cluster Regulator IscR in Response to Oxidative Stress and Iron Starvation
Jong Gyu Lim, Ye-Ji Bang, Sang Ho Choi
Journal of Biological Chemistry.2014; 289(52): 36263. CrossRef
- Environmental magnesium ion affects global gene expression, motility, biofilm formation and virulence of Vibrio parahaemolyticus
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