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- Genomic and physiological analysis of C50 carotenoid-producing novel Halorubrum ruber sp. nov.
- Chi Young Hwang , Eui-Sang Cho , Won Jong Rhee , Eunjung Kim , Myung-Ji Seo
- J. Microbiol. 2022;60(10):1007-1020. Published online August 26, 2022
- DOI: https://doi.org/10.1007/s12275-022-2173-1
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Abstract
- A novel haloarchaeal species designated as MBLA0099T was isolated from seawater near Yeongheung Island. Cells were Gram-negative, non-motile, red-pigmented, and rod-shaped. They grew at 10–45°C, within pH 5.5–9.0, and between 7.5% and 30% NaCl concentrations. Cells were able to grow without Mg2+ and were lysed in distilled water. The size of the whole-genome and G + C content of DNA was 3.02 Mb and 68.9 mol%, respectively. Phylogenetic analysis shows that the strain MBLA0099T belongs to the genus Halorubrum. The average nucleotide and amino acid identity, and in silico DNA-DNA hybridization values were below the species delineation threshold. Pan-genomic analysis revealed that 3.2% of all genes present in strain MBLA0099T were unique to the strain. The red carotenoid produced by strain MBLA0099T was subjected to spectrometric and chromatographic analyses and confirmed to be bacterioruberin as C50 carotenoid. Mevalonic acid, terpenoid backbone, and carotenoid biosynthesis pathway were annotated for strain MBLA0099T. The C50 carotenoid production by strain MBLA0099T was also enhanced under various stress conditions including relatively netural pH, high oxidative and salinity conditions. Additionally, the strain MBLA0099T-derived bacterioruberin showed the antioxidant activity with EC50 value of 12.29 μg/ml, based on the evaluation of DPPH free radical scavenging activity. The present study would be the first report on the identification of C50 carotenoid from the strain MBLA0099T representing a novel species of the genus Halorubrum, for which the name Halorubrum ruber sp. nov. is proposed. The typestrain used was MBLA0099T (= KCTC 4296T = JCM 34701T).
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Citations
Citations to this article as recorded by
- Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potential
Chi Young Hwang, Eui-Sang Cho, Sungjun Kim, Kyobum Kim, Myung-Ji Seo
Microbial Cell Factories.2024;[Epub] CrossRef - Microbial Bacterioruberin: A Comprehensive Review
Mouliraj Palanisamy, Sathishkumar Ramalingam
Indian Journal of Microbiology.2024; 64(4): 1477. CrossRef - Congregibacter variabilis sp. nov. and Congregibacter brevis sp. nov. Within the OM60/NOR5 Clade, Isolated from Seawater, and Emended Description of the Genus Congregibacter
Hyeonsu Tak, Miri S. Park, Hyerim Cho, Yeonjung Lim, Jang-Cheon Cho
Journal of Microbiology.2024; 62(9): 739. CrossRef - Genomic insights on carotenoid synthesis by extremely halophilic archaea Haloarcula rubripromontorii BS2, Haloferax lucentense BBK2 and Halogeometricum borinquense E3 isolated from the solar salterns of India
Devika. N. Nagar, Kabilan Mani, Judith M. Braganca
Scientific Reports.2024;[Epub] CrossRef - Microbial bacterioruberin: The new C50 carotenoid player in food industries
Cassamo U. Mussagy, Angie V. Caicedo-Paz, Fabiane O. Farias, Leonardo M. de Souza Mesquita, Daniele Giuffrida, Laurent Dufossé
Food Microbiology.2024; 124: 104623. CrossRef - Evaluation of Various Escherichia coli Strains for Enhanced Lycopene Production
Jun Ren, Junhao Shen, Thi Duc Thai, Min-gyun Kim, Seung Ho Lee, Wonseop Lim, Dokyun Na
Journal of Microbiology and Biotechnology.2023; 33(7): 973. CrossRef - Bioactive molecules from haloarchaea: Scope and prospects for industrial and therapeutic applications
Jamseel Moopantakath, Madangchanok Imchen, V. T. Anju, Siddhardha Busi, Madhu Dyavaiah, Rosa María Martínez-Espinosa, Ranjith Kumavath
Frontiers in Microbiology.2023;[Epub] CrossRef
- Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potential
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