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- Functional analysis of Vibrio vulnificus RND efflux pumps homologous to Vibrio cholerae VexAB and VexCD, and to Escherichia coli AcrAB
- Seunghwa Lee , Ji-Hyun Yeom , Sojin Seo , Minho Lee , Sarang Kim , Jeehyeon Bae , Kangseok Lee , Jihwan Hwang
- J. Microbiol. 2015;53(4):256-261. Published online March 4, 2015
- DOI: https://doi.org/10.1007/s12275-015-5037-0
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Abstract
- Resistance-nodulation-division (RND) efflux pumps are associated with multidrug resistance in many gram-negative pathogens. The genome of Vibrio vulnificus encodes 11 putative RND pumps homologous to those of Vibrio cholerae and Escherichia coli. In this study, we analyzed three putative RND efflux pumps, showing homology to V. cholerae VexAB and VexCD and to E. coli AcrAB, for their functional roles in multidrug resistance of V. vulnificus. Deletion of the vexAB homolog resulted in increased susceptibility of V. vulnificus to bile acid, acriflavine, ethidium bromide, and erythromycin, whereas deletion of acrAB homologs rendered V. vulnificus more susceptible to acriflavine only. Deletion of vexCD had no effect on susceptibility of V. vulnificus to these chemicals. Upon exposure to these antibacterial chemicals, expression of tolCV1 and tolCV2, which are putative outer membrane factors of RND efflux pumps, was induced, whereas expression levels of vexAB, vexCD, and acrAB homologs were not significantly changed. Our results show that the V. vulnificus homologs of VexAB largely contributed to in vitro antimicrobial resistance with a broad substrate specificity that was partially redundant with the AcrAB pump homologs.
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Citations
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- Dynamics of efflux pumps in antimicrobial resistance, persistence, and community living of Vibrionaceae
Sanath Kumar, Manjusha Lekshmi, Jerusha Stephen, Anely Ortiz-Alegria, Matthew Ayitah, Manuel F. Varela
Archives of Microbiology.2024;[Epub] CrossRef - Membrane Efflux Pumps of Pathogenic Vibrio Species: Role in Antimicrobial Resistance and Virulence
Jerusha Stephen, Manjusha Lekshmi, Parvathi Ammini, Sanath H. Kumar, Manuel F. Varela
Microorganisms.2022; 10(2): 382. CrossRef - TolCV1 Has Multifaceted Roles During Vibrio vulnificus Infection
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Microbial Genomics .2018;[Epub] CrossRef - ATP-Binding Cassette Transporter VcaM from Vibrio cholerae is Dependent on the Outer Membrane Factor Family for Its Function
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International Journal of Molecular Sciences.2018; 19(4): 1000. CrossRef - Contribution of efflux systems to the detergent resistance, cytotoxicity, and biofilm formation of Vibrio vulnificus
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Clinical Microbiology Reviews.2016; 29(4): 819. CrossRef - MdsABC-Mediated Pathway for Pathogenicity in Salmonella enterica Serovar Typhimurium
Saemee Song, Boeun Lee, Ji-Hyun Yeom, Soonhye Hwang, Ilnam Kang, Jang-Cheon Cho, Nam-Chul Ha, Jeehyeon Bae, Kangseok Lee, Yong-Hak Kim, S. M. Payne
Infection and Immunity.2015; 83(11): 4266. CrossRef - Molecular architecture of the bacterial tripartite multidrug efflux pump focusing on the adaptor bridging model
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Journal of Microbiology.2015; 53(6): 355. CrossRef
- Dynamics of efflux pumps in antimicrobial resistance, persistence, and community living of Vibrionaceae
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