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- Isolation of a novel strain, Sphingorhabdus sp. YGSMI21 and characterization of its enantioselective epoxide hydrolase activity
- Jung-Hee Woo , Hae-Seon Kim , Nyun-Ho Park , Ho Young Suk
- J. Microbiol. 2021;59(7):675-680. Published online June 1, 2021
- DOI: https://doi.org/10.1007/s12275-021-1023-x
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Abstract
- Sphingorhabdus sp. YGSMI21, a novel microbial strain with an enantioselective epoxide hydrolase activity, was isolated from tidal samples contaminated by accidental oil spills subjected to enriched culture with polycyclic aromatic hydrocarbon. This strain was able to optically decompose (R)-styrene oxide (SO) and showed 100% optical purity. In addition, it showed a good enantioselectivity for the derivatives of (S)- SO, (S)-2-chlorostyrene oxide (CSO), (S)-3-CSO and (S)-4- CSO. For (S)-2-CSO, (S)-3-CSO and (S)-4-CSO, 99.9%ee was obtained with the yield of 26.2%, 24.8%, and 11.0%, respectively, when using 10 mg cells of Sphingorhabdus sp. YGSMI21 at pH 8.0 with 4 mM racemic substrates at pH 8.0 and 25°C. The values obtained in this study for (S)-2-CSO, particularly the yield of 26.2%, is noteworthy, considering that obtaining an enantiomerically pure form is difficult. Taken together, Sphingorhabdus sp. YGSMI21 can be regarded as a wholecell biocatalyst in the production of various (S)-CSO with the chlorine group at a different position.
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Citations
Citations to this article as recorded by
- Epoxide Hydrolases: Multipotential Biocatalysts
Marek Bučko, Katarína Kaniaková, Helena Hronská, Peter Gemeiner, Michal Rosenberg
International Journal of Molecular Sciences.2023; 24(8): 7334. CrossRef - Effects of submerged macrophytes (Elodea nuttallii) on water quality and microbial communities of largemouth bass (Micropterus salmoides) ponds
Zhijuan Nie, Zhaowei Zheng, Haojun Zhu, Yi Sun, Jun Gao, Jiancao Gao, Pao Xu, Gangchuan Xu
Frontiers in Microbiology.2023;[Epub] CrossRef - Description of Polaribacter batillariae sp. nov., Polaribacter cellanae sp. nov., and Polaribacter pectinis sp. nov., novel bacteria isolated from the gut of three types of South Korean shellfish
Su-Won Jeong, Jeong Eun Han, June-Young Lee, Ji-Ho Yoo, Do-Yeon Kim, In Chul Jeong, Jee-Won Choi, Yun-Seok Jeong, Jae-Yun Lee, So-Yeon Lee, Euon Jung Tak, Hojun Sung, Hyun Sik Kim, Pil Soo Kim, Dong-Wook Hyun, Jin-Woo Bae
Journal of Microbiology.2022; 60(6): 576. CrossRef
- Epoxide Hydrolases: Multipotential Biocatalysts
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