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- Isolation and genome analysis of Winogradskyella algicola sp. nov., the dominant bacterial species associated with the green alga Dunaliella tertiolecta
- Jaeho Song , Yeonjung Lim , Hye-Jin Jang , Yochan Joung , Ilnam Kang , Seong-Joo Hong , Choul-Gyun Lee , Jang-Cheon Cho
- J. Microbiol. 2019;57(11):982-990. Published online October 28, 2019
- DOI: https://doi.org/10.1007/s12275-019-9378-y
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Abstract
- Microalgae and bacteria are known to be closely associated in diverse environments. To isolate dominant bacterial species associated with a green alga, Dunaliella tertiolecta, a photoreactor culture of the microalga was investigated using culture- based and culture-independent approaches. The bacterial community structure of the algal culture showed that the most abundant bacterial species under the culture conditions was related to the genus Winogradskyella. The closely related amplicon sequences, showing ≥ 99.5% 16S rRNA gene sequence similarity to one of the isolates, designated IMCC- 33238T, constituted > 49% of the bacterial community and was therefore regarded as the most dominant species in the algal culture. Strain IMCC33238T was characterized by Gramstaining- negative and orange-colored rods. Phylogenetic analyses of the 16S rRNA genes as well as whole genome sequences revealed that strain IMCC33238T belonged to Winogradskyella and shared more than 97.2% 16S rRNA gene sequence similarity with Winogradskyella species. The strain contained iso-C15:1 G, iso-C15:0, iso-C15:0 3-OH, and summed feature 3 (C16:1 ω6c and/or C16:1 ω7c) as major fatty acids and MK-6 as the predominant quinone. The polar lipids found in strain IMCC33238T were phosphatidylethanolamine, two unidentified aminolipids, and three unidentified lipids. The genome of strain IMCC33238T was 3.37 Mbp in size with 33.9 mol% G + C content and proteorhodopsin. Many genes encoding folate and vitamin production are considered to play an important role in the bacteria-algae interaction. On the basis of phylogenetic and phenotypic characteristics, strain IMCC33238T represents a novel species in the genus Winogradskyella, for which the name Winogradskyella algicola sp. nov. is proposed. The type strain is IMCC33238T (= KACC 21192T = NBRC 113704T).
- NOTE] Winogradskyella jejuensis sp. nov., a Marine Bacterium Isolated from a Brown Alga Carpopeltis affinis
- Ji-Young Kim , Duck-Chul Oh
- J. Microbiol. 2012;50(5):888-892. Published online November 4, 2012
- DOI: https://doi.org/10.1007/s12275-012-2447-0
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Abstract
- A Gram-negative, orange-pigmented, rod-shaped bacterium, designated strain CP32T was isolated from a brown alga Carpopeltis affinis collected on the coast of Jeju Island, Republic of Korea. The isolate grew at 10–37°C (optimum 25°C) and at pH 6.5–9.5 (optimum pH 7.0). The 16S rRNA gene sequence of the isolate showed much similarity with the type strains of recognized species of the genus Winogradskyella (94.0–96.6%). The most closely related species were Winogradskyella echinorum KMM 6211T, Winogradskyella ulvae KMM 6390T, Winogradskyella thalassocola KMM 3907T, Winogradskyella poriferorum UST030701-295T, and Winogradskyella eximia KMM 3944T. The major respiratory quinone was menaquinone-6 (MK-6) and the predominant cellular fatty acids were iso-C15:1 G (24.8%), iso-C15:0 (23.4%), and iso-C17:0 3-OH (11.6 %). The DNA G+C content was 33.3 mol%. The polar lipid profile was composed of phosphatidylethanolamine, two aminolipids, and five unknown lipids. On the basis of phenotypic features, and the result of 16S rRNA gene sequence analysis, strain CP32T (=KCTC 23835T =JCM 18454T) represents a novel species of the genus Winogradskyella, for which the name Winogradskyella jejuensis sp. nov. is proposed.
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