Journal of Microbiology

Cloning of the gense coding for extracellular proteases from alkalophilic xanthomonas SP. JK311: Kim, Young Hun , Jang, Ji Yeon , Yeeh, Yeehn , Kim, Yong Ho , Kim, Sang Hae; J. Microbiol. 1995;33(4):344-349.

Abstract: The alkalophilic bacterium, Xanthomonas sp. JK311, producing extracellular proteases, was isolated from soil. Xanthomonas sp. JK311 produced five extracellular proteases that are all metalloproteases. Four of them were resistant against 1% SDS. Chromosomal DNA of the Xanthomonas sp. JK311 was digested with BamHI and cloned into PUC19. Among E. coli strain HB101 transformants, a clone secreting the proteases was screened through halo formation on skim-milk agar plate and by Southern blot analysis. It had the recombinant plasmid pXEP-1 containing the 7.5 kb-BamHI DNA fragment and produced three extacellular proteases. Their protease properties corresponded to those of Xanthomonas sp. JK311.

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