Journal of Microbiology

Journal Article

Characterization of staphylococcal endolysin LysSAP33 possessing untypical domain composition: Jun-Hyeok Yu , Do-Won Park , Jeong-A Lim , Jong-Hyun Park; J. Microbiol. 2021;59(9):840-847. Published online August 12, 2021; DOI: https://doi.org/10.1007/s12275-021-1242-1

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Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent. Many endolysins on staphylococcal phages have been identified and applied extensively against Staphylococcus spp. Among them, LysK-like endolysin, a well-studied staphylococcal endolysin, accounts for most of the identified endolysins. However, relatively little interest has been paid to LysKunlike endolysin and a few of them has been characterized. An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD). A green fluorescence assay using a fusion protein for Lys- SAP33_CBD indicated that the CBD domain (157-251 aa) was bound to the peptidoglycan of S. aureus. The deletion of LysSAP33_CBD at the C-terminal region resulted in a significant decrease in lytic activity and efficacy. Compared to LysK-like endolysin, LysSAP33 retained its lytic activity in a broader range of temperature, pH, and NaCl concentrations. In addition, it showed a higher activity against biofilms than LysK-like endolysin. This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.

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Phage-Derived Endolysins Against Resistant Staphylococcus spp.: A Review of Features, Antibacterial Activities, and Recent Applications
Mina Golban, Javad Charostad, Hossein Kazemian, Hamid Heidari
Infectious Diseases and Therapy.2024;[Epub] CrossRef
Molecular Machinery of the Triad Holin, Endolysin, and Spanin: Key Players Orchestrating Bacteriophage-Induced Cell Lysis and their Therapeutic Applications
Safia Samir
Protein & Peptide Letters.2024; 31(2): 85. CrossRef
A Novel Truncated CHAP Modular Endolysin, CHAPSAP26-161, That Lyses Staphylococcus aureus, Acinetobacter baumannii, and Clostridioides difficile, and Exhibits Therapeutic Effects in a Mouse Model of A. baumannii Infection
Yoon-Jung Choi, Shukho Kim, Ram Hari Dahal, Jungmin Kim
Journal of Microbiology and Biotechnology.2024; 34(8): 1718. CrossRef
Therapeutic potential of bacteriophage endolysins for infections caused by Gram-positive bacteria
He Liu, Zhen Hu, Mengyang Li, Yi Yang, Shuguang Lu, Xiancai Rao
Journal of Biomedical Science.2023;[Epub] CrossRef
Endolysin, a Promising Solution against Antimicrobial Resistance
Mujeeb ur Rahman, Weixiao Wang, Qingqing Sun, Junaid Ali Shah, Chao Li, Yanmei Sun, Yuanrui Li, Bailing Zhang, Wei Chen, Shiwei Wang
Antibiotics.2021; 10(11): 1277. CrossRef

Research Support, Non-U.S. Gov'ts

Pb Tolerance and Bioaccumulation by the Mycelia of Flammulina velutipes in Artificial Enrichment Medium: Changwei Zhu , Zhengpeng Li , Decai Li , Yan Xin; J. Microbiol. 2014;52(1):8-12. Published online January 4, 2014; DOI: https://doi.org/10.1007/s12275-014-2560-3

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Abstract

Mushrooms have the ability to accumulate high concentrations of heavy metals, which gives them potential for use as bioremediators of environmental contamination. The Pb2+ tolerance and accumulation ability of living mycelia of Flammulina velutipes were studied in this work. Mycelial growth was inhibited when exposed to 1 mM Pb2+. The colony diameter on solid medium decreased almost 10% compared with the control. Growth decreased almost 50% when the Pb2+ concentration increased to 4 mM in the medium, with the colony diameter decreasing from 80 mm to 43.4 mm, and dry biomass production in liquid cultures decreasing from 9.23±0.55 to 4.27±0.28 g/L. Lead ions were efficiently accumulated in the mycelia. The amount of Pb2+ in the mycelia increased with increasing Pb2+ concentration in the medium, with the maximum concentration up to 707±91.4 mg/kg dry weight. We also show evidence that a large amount of the Pb2+ was adsorbed to the mycelial surface, which may indicate that an exclusion mechanism is involved in Pb tolerance. These results demonstrate that F. velutipes could be useful as a remediator of heavy metal contamination because of the characteristics of high tolerance to Pb2+ and efficient accumulation of Pb2+ ions by the mycelia.

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An Approach to Evaluate Pb Tolerance and Its Removal Mechanisms by Pleurotus opuntiae
Priyanka Yadav, Vartika Mishra, Tejmani Kumar, Awadhesh Kumar Rai, Ayush Gaur, Mohan Prasad Singh
Journal of Fungi.2023; 9(4): 405. CrossRef
Detrimental and essential elements in fruiting bodies of wild-growing fungi Coprinus comatus, Flammulina velutipes, and Armillaria ostoyae
Jan Vondruška, Jan Šíma, Marek Kobera, Lukáš Rokos, Martin Šeda, Lubomír Svoboda
Journal of Environmental Science and Health, Part B.2022; 57(4): 243. CrossRef
Waste apple wood: A safe and economical alternative substrate for the cultivation of Pleurotus ostreatus and Lentinula edodes
Guorong Yu, Xinxin Li, Shengting Zhao, Shuguang Sun, Yanru Yu, Jinzhi Chen, Xianhao Cheng, Weihuan Li
Folia Horticulturae.2022; 34(2): 173. CrossRef
The effect of different substrates on the growth of six cultivated mushroom species and composition of macro and trace elements in their fruiting bodies
Marek Siwulski, Piotr Rzymski, Anna Budka, Pavel Kalač, Sylwia Budzyńska, Luiza Dawidowicz, Edmund Hajduk, Lidia Kozak, Joanna Budzulak, Krzysztof Sobieralski, Przemysław Niedzielski
European Food Research and Technology.2019; 245(2): 419. CrossRef
Biosorption characteristic of Alcaligenes sp. BAPb.1 for removal of lead(II) from aqueous solution
Yu Jin, Sumei Yu, Chunying Teng, Tao Song, Liying Dong, Jinsong Liang, Xin Bai, Xiuhong Xu, Juanjuan Qu
3 Biotech.2017;[Epub] CrossRef

Expression Analysis of LeNHX1 Gene in Mycorrhizal Tomato under Salt Stress: ZhongQun He , Zhi Huang; J. Microbiol. 2013;51(1):100-104. Published online March 2, 2013; DOI: https://doi.org/10.1007/s12275-013-2423-3

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Abstract

The plant growth, stem sap flow, Na+ and Cl- content, and the expression of vacuolar Na+/H+ antiporter gene (LeNHX1) in the leaves and roots of tomato under different NaCl stresses (0.5% and 1%) were studied to analyze the effect of arbuscular mycorrhizal fungi (AMF) on Na+ and Cl- accumulation and ion exchange. The results showed that arbuscular mycorrhizal (AM) plant growth and stem sap flow increased and salt tolerance improved, whereas Na+ and Claccumulated. Na+ significantly decreased, and no significant decline was detected in Cl- content after AMF inoculation compared with the non-AM plants. The LeNHX1 gene expression was induced in the AM and non-AM plants by NaCl stress. However, AMF did not improve the LeNHX1 level, and low expression was observed in the AM tomato. Hence, the mechanism that reduced the Na+ damage to tomato induced by AMF has little relation to LeNHX1, which can export Na+ from the cytosol to the vacuole across the tonoplast.

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Context-dependent contributions of arbuscular mycorrhizal fungi to host performance under global change factors
Lennel Camuy-Velez, Ditam Chakraborty, Addisyn Young, Sakshi Paudel, Rylie Elvers, Miranda Vanderhyde, Kelly Walter, Chantal Herzog, Samiran Banerjee
Soil Biology and Biochemistry.2025; 204: 109707. CrossRef
Alleviation of drought and salt stress in vegetables: crop responses and mitigation strategies
Muhammad Fasih Khalid, Samsul Huda, Miingtiem Yong, Lihua Li, Li Li, Zhong-Hua Chen, Talaat Ahmed
Plant Growth Regulation.2023; 99(2): 177. CrossRef
Insights into the molecular aspects of salt stress tolerance in mycorrhizal plants
Bhawna Saxena, Karuna Sharma, Rupam Kapoor, Qiang-Sheng Wu, Bhoopander Giri
World Journal of Microbiology and Biotechnology.2022;[Epub] CrossRef
Infection by phloem-limited phytoplasma affects mineral nutrient homeostasis in tomato leaf tissues
Sara Buoso, Rita Musetti, Fabio Marroni, Alberto Calderan, Wolfgang Schmidt, Simonetta Santi
Journal of Plant Physiology.2022; 271: 153659. CrossRef
Diversity of arbuscular mycorrhiza fungi in rhizosphere soil and roots in Vetiveria zizanioides plantation chronosequence in coal gangue heaps
Tianyue Gu, Yuanyuan Mao, Chao Chen, Yong Wang, Qi Lu, Huaqiu Wang, Wei Cheng
Symbiosis.2022; 86(1): 111. CrossRef
Physiological and molecular responses of wheat plants to mycorrhizal and epibrassinolide interactions under salinity
Cobra Tofighi, Ramazan Ali Khavari-Nejad, Farzaneh Najafi, Khadijeh Razavi, Farhad Rejali
Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology.2021; 155(5): 1075. CrossRef
Tapping the potential of Solanum lycopersicum L. pertaining to salinity tolerance: perspectives and challenges
Sarvesh Pratap Kashyap, Nishi Kumari, Pallavi Mishra, Durga Prasad Moharana, Mohd Aamir
Genetic Resources and Crop Evolution.2021; 68(6): 2207. CrossRef
Alleviation of Salt Stress in Upland Rice (Oryza sativa L. ssp. indica cv. Leum Pua) Using Arbuscular Mycorrhizal Fungi Inoculation
Rujira Tisarum, Cattarin Theerawitaya, Thapanee Samphumphuang, Kanyamin Polispitak, Panarat Thongpoem, Harminder Pal Singh, Suriyan Cha-um
Frontiers in Plant Science.2020;[Epub] CrossRef
Do halophytes and glycophytes differ in their interactions with arbuscular mycorrhizal fungi under salt stress? A meta-analysis
Jing Pan, Fei Peng, Anna Tedeschi, Xian Xue, Tao Wang, Jie Liao, Wenjuan Zhang, Cuihua Huang
Botanical Studies.2020;[Epub] CrossRef
Salinity tolerance and sodium localization in mycorrhizal strawberry plants
Shiam Ibna Haque, Yoh-ichi Matsubara
Communications in Soil Science and Plant Analysis.2018; 49(22): 2782. CrossRef
Whole-genome identification and expression analysis of K+ efflux antiporter (KEA) and Na+/H+ antiporter (NHX) families under abiotic stress in soybean
Hua-tao CHEN, Xin CHEN, Bing-yue WU, Xing-xing YUAN, Hong-mei ZHANG, Xiao-yan CUI, Xiao-qing LIU
Journal of Integrative Agriculture.2015; 14(6): 1171. CrossRef
Increasing the productivity and product quality of vegetable crops using arbuscular mycorrhizal fungi: A review
C. Baum, W. El-Tohamy, N. Gruda
Scientia Horticulturae.2015; 187: 131. CrossRef

Dark Septate Endophyte (DSE) Fungi Isolated from Metal Polluted Soils: Their Taxonomic Position, Tolerance, and Accumulation of Heavy Metals In Vitro: Yujie Zhang , Yan Zhang , Maojun Liu , Xiaodong Shi , Zhiwei Zhao; J. Microbiol. 2008;46(6):624-632. Published online December 24, 2008; DOI: https://doi.org/10.1007/s12275-008-0163-6

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Abstract: To understand the possible role of the plant root associated fungi on metal tolerance, their role in the uptake of heavy metals and the potential transfer of these metal ions to the plant, three strains of dark septate endophytic (DSE) fungi were isolated from a waste smelter site in southwest China, and one strain was isolated from a non-contaminated site. According to molecular phylogenetic analysis of the ITS 1-5.8S rDNA-ITS 2 gene regions and morphological characteristics, one is identified as Exophiala pisciphila, and the other three are non-sporulating fungi under the experiment condition with the nearest phylogenetic affinities to the Thysanorea papuana strain EU041814. Tolerance and accumulation abilities of the three DSE strains for metals were investigated in liquid culture. Minimum inhibitory concentrations (MIC) of Pb, Zn, and Cd were determined. It was demonstrated that the tolerance of the DSE strains varied between metal species and strains. The E. pisciphila strain is able to accumulate lead and cadmium over 20% and 5% of dry weight of biomass, respectively. Partial of the sequestrated metals can be washed with CaCl2. Morphological and enzyme activity changes taking place in the presence of excessive Pb, Cd, and/or Zn also indicate that the mechanism of heavy metal tolerance and accumulation of the DSE strains would be a complex process. The findings indicated promising tolerance and accumulation of the DSE strains with potential values in metal cycling and restoration of soil and water system.

Adaptaion of Azomonas agilis PY101 Exposed to Cadmium vua Production of Cadmium-Binding Pigment Promoted by Cd^2+: You, Kyung Man , Park, Yong Keun; J. Microbiol. 1998;36(3):159-163.

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Abstract: Azomonas agilis PY101 produced a fluorescent yellow-green pigment promoted by cadmium. The amount of extracellular pigment produced during the growth of A. agilis PY101 increased to approximately 6 times its initial value after the addition to 1.0 mg/ml of CdCl_2. The pigment peak(peak II) was observed when the supernatant solution acquired from the cells cultivated in the presence of cadmium was fractionated on a column of Superdex 75. Peak II contained about 70% of extracellular cadmium in the supernatant solution. This cadmium-binding pigment contained several sulfur-containing groups. The dramatic decrease (97%) of sulfate ion (SO_4^-2)concentration in the cytoplasm from 9.60 to 0.25 ㎍/ml during the growth of A. agilis PY101 under cadmium stress was confirmed by ion chromatography. Moreover, transmission electron microscopic analysis showed that Z. agilis PY101 actively accumulated cadmium in the interior of the cells. It appears that the cadmium adaptation of A. agilis PY101 is achieved by the microbial binding of the sulfur-containing pigment to cadmium.

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