Research Support, Non-U.S. Gov't
- Occurrence and Antimicrobial Drug Susceptibility Patterns of Commensal and Diarrheagenic Escherichia coli in Fecal Microbiota from Children with and without Acute Diarrhea
-
Patrícia G. Garcia , Vânia L. Silva , Cláudio G. Diniz
-
J. Microbiol. 2011;49(1):46-52. Published online March 3, 2011
-
DOI: https://doi.org/10.1007/s12275-011-0172-8
-
-
41
View
-
0
Download
-
36
Crossref
-
Abstract
-
Acute diarrhea is a public health problem and an important cause of morbidity and mortality, especially in developing countries. The etiology is varied, and the diarrheagenic Escherichia coli pathotypes are among the most important. Our objectives were to determine the occurrence of commensal and diarrheagenic E.
coli strains in fecal samples from children under five years old and their drug susceptibility patterns. E. coli were isolated from 141 fresh fecal samples; 84 were obtained from clinically injured donors with acute diarrhea (AD) and 57 from clinically healthy donors without diarrhea (WD). Presumptive phenotypic species
identification was carried out and confirmed by amplification of specific 16S ribosomal RNA encoding DNA. Multiplex PCR was performed to characterize the diarrheagenic E. coli strains. Drug susceptibility patterns were determined by the disc-diffusion method. In total, 220 strains were recovered from the fecal specimens
(61.8% from AD and 38.2% from WD). Diarrheagenic E. coli was identified at a rate of 36.8% (n=50) in diarrheic feces and 29.8% (n=25) in non-diarrheic feces. Enteroaggregative E. coli was the most frequently identified pathotype in the AD group (16.2%) and the only pathotype identified in the WD group (30.9%).
Enteropathogenic E. coli was the second most isolated pathotype (10.3%), followed by Shiga toxin-producing E. coli (7.4%) and enterotoxigenic E. coli (2.9%). No enteroinvasive E. coli strains were recovered. The isolates showed high resistance rates against ampicillin, tetracycline, and sulfamethoxazole-trimethoprim.
The most effective drugs were ceftazidime, ceftriaxone, imipenem and piperacillin-tazobactam, for which no resistance was observed. Differentiation between the diarrheagenic E. coli pathotypes is of great importance since they are involved in acute diarrheal diseases and may require specific antimicrobial chemotherapy. The high antimicrobial resistance observed in our study raises a broad discussion on the
indiscriminate or improper use of antimicrobials, besides the risks of self-medication.
-
Citations
Citations to this article as recorded by

- Characterization of Antimicrobial Resistance in Escherichia coli Isolated from Diarrheic and Healthy Weaned Pigs in Catalonia
Biel Garcias, Marga Martin, Laila Darwich
Animals.2024; 14(3): 487. CrossRef - Synthesis of Fe–Pr co-doped ZnO nanoparticles: Structural, optical and antibacterial properties
M. Rocha, Francisca P. Araujo, S. Castro-Lopes, Idglan S. de Lima, Edson Cavalcanti Silva-Filho, Josy Anteveli Osajima, Carla S. Oliveira, Bartolomeu C. Viana, Luciano C. Almeida, Y. Guerra, R. Peña-Garcia
Ceramics International.2023; 49(2): 2282. CrossRef - Antibiotic resistance trends for common bacterial aetiologies of childhood diarrhoea in low- and middle-income countries: A systematic review
Raghavee Neupane, Myra Bhathena, Gopika Das, Elizabeth Long, Jennifer Beard, Hiwote Solomon, Jon L Simon, Yasir B Nisar, William B MacLeod, Davidson H Hamer
Journal of Global Health.2023;[Epub] CrossRef - Comparative genomics analysis and characterization of Shiga toxin-producing Escherichia coli O157:H7 strains reveal virulence genes, resistance genes, prophages and plasmids
Natalie Naidoo, Oliver T. Zishiri
BMC Genomics.2023;[Epub] CrossRef - Prevalence of Tetracycline Resistance Genes tet (A, B, C, 39) in Klebsiella pneumoniae Isolated from Tehran, Iran
Zeinab Ahmadi, Zahra Noormohammadi, Reza Ranjbar, Payam Behzadi
Iranian Journal of Medical Microbiology.2022; 16(2): 141. CrossRef - Supershed Escherichia coli O157:H7 Has Potential for Increased Persistence on the Rectoanal Junction Squamous Epithelial Cells and Antibiotic Resistance
Raies A. Mir, Brian W. Brunelle, David P. Alt, Terrance M. Arthur, Indira T Kudva
International Journal of Microbiology.2020; 2020: 1. CrossRef - Antimicrobial resistance and prevalence of tetracycline resistance genes in Escherichia coli isolated from lesions of colibacillosis in broiler chickens in Sistan, Iran
Mohammad Jahantigh, Keyvan Samadi, Reza Esmaeelzadeh Dizaji, Saeed Salari
BMC Veterinary Research.2020;[Epub] CrossRef - Shiga-Toxin Producing Escherichia Coli in Brazil: A Systematic Review
Vinicius Silva Castro, Eduardo Eustáquio de Souza Figueiredo, Kim Stanford, Tim McAllister, Carlos Adam Conte-Junior
Microorganisms.2019; 7(5): 137. CrossRef - Antibiotic‐resistant Shiga toxin‐producing Escherichia coli: An overview of prevalence and intervention strategies
Raies A. Mir, Indira T. Kudva
Zoonoses and Public Health.2019; 66(1): 1. CrossRef - Diarrheagenic Escherichia coli in Costa Rican children: a 9-year retrospective study
Cristian Pérez-Corrales, Kevin Leandro-Sandí
BMC Research Notes.2019;[Epub] CrossRef - Comparison of Commensal Escherichia coli Isolates from Adults and Young Children in Lubuskie Province, Poland: Virulence Potential, Phylogeny and Antimicrobial Resistance
Ewa Bok, Justyna Mazurek, Andrzej Myc, Michał Stosik, Magdalena Wojciech, Katarzyna Baldy-Chudzik
International Journal of Environmental Research and Public Health.2018; 15(4): 617. CrossRef - Molecular Diagnosis of Diarrheagenic E. coli Infections Among the Pediatric Patients in Wasit Province, Iraq
Zainab Kadhim Abdul-hussein, Rana Hussein Raheema, Ahmed Ibrahim Inssaf
Journal of Pure and Applied Microbiology.2018; 12(4): 2229. CrossRef - Real-Time Multiplex Polymerase Chain Reaction with High-Resolution Melting-Curve Analysis for the Diagnosis of Enteric Infections Associated with Diarrheagenic Escherichia coli
Thingujam Surbala Devi, Elantamilan Durairaj, Wihiwot Valarie Lyngdoh, Sourabh Gohain Duwarah, Annie Bakorlin Khyriem, Clarissa Jane Lyngdoh
Indian Journal of Medical Microbiology.2018; 36(4): 547. CrossRef - Alterations of Intestinal Microbiome by Antibiotic Therapy in Hospitalized Children
Miriam R. Fernandes, Aline Ignacio, Viviane A.A. Rodrigues, Franciso C. Groppo, Ary L Cardoso, Mario J. Avila-Campos, Viviane Nakano
Microbial Drug Resistance.2017; 23(1): 56. CrossRef - Clinical features and molecular epidemiology of diarrheagenic Escherichia coli pathotypes identified by fecal gastrointestinal multiplex nucleic acid amplification in patients with cancer and diarrhea
Andrew W. Chao, Micah Bhatti, Herbert L. DuPont, James P. Nataro, Lily G. Carlin, Pablo C. Okhuysen
Diagnostic Microbiology and Infectious Disease.2017; 89(3): 235. CrossRef - Presence and Antimicrobial Resistance of Escherichia coli in Ready-to-Eat Foods in Shaanxi, China
Allah Bux Baloch, Hua Yang, Yuqing Feng, Meili Xi, Qian Wu, Qinhao Yang, Jingsi Tang, Xiangxiang He, Yingping Xiao, Xiaodong Xia
Journal of Food Protection.2017; 80(3): 420. CrossRef - High prevalence of multidrug-resistant Escherichia coli isolates from children with and without diarrhoea and their susceptibility to the antibacterial activity of extracts/fractions of fruits native to Mexico
Magdalena de Jesús Uribe-Beltrán, Yesmi Patricia Ahumada-Santos, Sylvia Páz Díaz-Camacho, Carlos Alberto Eslava-Campos, Jesús Ernesto Reyes-Valenzuela, María Elena Báez-Flores, Ignacio Osuna-Ramírez, Francisco Delgado-Vargas
Journal of Medical Microbiology
.2017; 66(7): 972. CrossRef - Longitudinal Comparison of Antibiotic Resistance in Diarrheagenic and Non-pathogenic Escherichia coli from Young Tanzanian Children
Jessica C. Seidman, Lashaunda B. Johnson, Joshua Levens, Harran Mkocha, Beatriz Muñoz, Ellen K. Silbergeld, Sheila K. West, Christian L. Coles
Frontiers in Microbiology.2016;[Epub] CrossRef - Prevalence and Antimicrobial Resistance Patterns of Diarrheagenic Escherichia coli in Shanghai, China
Zheng Huang, Haijian Pan, Pingping Zhang, Xiaowei Cao, Wenting Ju, Chuanqing Wang, Jianmin Zhang, Jianghong Meng, Zhengan Yuan, Xuebin Xu
Pediatric Infectious Disease Journal.2016; 35(8): 835. CrossRef - Characterization of enteropathogenicEscherichia coliof clinical origin from the pediatric population in Pakistan
Mahwish Younas, Fariha Siddiqui, Zobia Noreen, Syeda Sadia Bokhari, Oscar G. Gomez-Duarte, Brendan W. Wren, Habib Bokhari
Transactions of The Royal Society of Tropical Medicine and Hygiene.2016; 110(7): 414. CrossRef - Molecular Detection of Diffusely Adherent Escherichia coli Strains Associated with Diarrhea in Shiraz, Iran
Pejman Abbasi, Mohammad Kargar, Abbas Doosti, Jalal Mardaneh, Sadegh Ghorbani-Dalini, Mohammad Ali Dehyadegari
Archives of Pediatric Infectious Diseases.2016;[Epub] CrossRef - Faecal carriage of antibiotic resistant Escherichia coli in asymptomatic children and associations with primary care antibiotic prescribing: a systematic review and meta-analysis
Ashley Bryce, Céire Costelloe, Claire Hawcroft, Mandy Wootton, Alastair D. Hay
BMC Infectious Diseases.2016;[Epub] CrossRef - Passive immunisation, an old idea revisited: Basic principles and application to modern animal production systems
Chris J. Hedegaard, Peter M.H. Heegaard
Veterinary Immunology and Immunopathology.2016; 174: 50. CrossRef - Epidemiology, Antimicrobial Resistance and β-lactamase Genotypic Features of Enteropathogenic Escherichia coli Isolated from Children with Diarrhea in Southern China
Yong Huang, Xue-feng Shan, Haijun Deng, Yu-jun Huang, Xiao-ping Mu, Ai-long Huang, Quan-xin Long
Japanese Journal of Infectious Diseases.2015; 68(3): 239. CrossRef - Diarrhoeagenic Escherichia coli in mother-child Pairs in Ile-Ife, South Western Nigeria
Babatunde W. Odetoyin, Jennifer Hofmann, Aaron O. Aboderin, Iruka N. Okeke
BMC Infectious Diseases.2015;[Epub] CrossRef - Genetic Relatedness Among Escherichia coli Pathotypes Isolated from Food Products for Human Consumption in Cartagena, Colombia
Zorangel Amézquita-Montes, Maria Tamborski, Usa G. Kopsombut, Chengxian Zhang, Octavio S. Arzuza, Oscar G. Gómez-Duarte
Foodborne Pathogens and Disease.2015; 12(5): 454. CrossRef - Characterization of the microbial community in a lotic environment to assess the effect of pollution on nitrifying and potentially pathogenic bacteria
JD Medeiros, LX Araújo, VL. da Silva, CG Diniz, DE Cesar, A Del'Duca, CM Coelho
Brazilian Journal of Biology.2014; 74(3): 612. CrossRef - Antimicrobial resistance of bacterial enteropathogens isolated from stools in Madagascar
Frederique Randrianirina, Elisoa Hariniana Ratsima, Lova Ramparany, Rindra Randremanana, Hanitra Clara Rakotonirina, Tahiry Andriamanantena, Fanjasoa Rakotomanana, Soatiana Rajatonirina, Vincent Richard, Antoine Talarmin
BMC Infectious Diseases.2014;[Epub] CrossRef - Prevalent Phenotypic and Genotypic Profile of Enterotoxigenic Escherichia coli among Iranian Children
Shahram Nazarian, Seyed Latif Mousavi Gargari, Iraj Rasooli, Masoome Alerasol, Samane Bagheri, Shakiba Darvish Alipoor
Japanese Journal of Infectious Diseases.2014; 67(2): 78. CrossRef - Epidemiology and Clinical Manifestations of Enteroaggregative Escherichia coli
Betina Hebbelstrup Jensen, Katharina E. P. Olsen, Carsten Struve, Karen Angeliki Krogfelt, Andreas Munk Petersen
Clinical Microbiology Reviews.2014; 27(3): 614. CrossRef - Molecular mechanisms of antibiotic resistance in diarrhoeagenic Escherichia coli isolated from children
Susan Mosquito, Joaquim Ruiz, María J. Pons, David Durand, Francesca Barletta, Theresa J. Ochoa
International Journal of Antimicrobial Agents.2012; 40(6): 544. CrossRef - Frequência de Escherichia coli e sua sensibilidade aos antimicrobianos em menores de cinco anos hospitalizados por diarreia aguda
Maria do Rosário S. de Almeida Lélis de Moura, Maria Júlia Gonçalves de Mello, Waldylene Barbosa Calábria, Eliane Mendes Germano, Ruben Rolando Schindler Maggi, Jailson de Barros Correia
Revista Brasileira de Saúde Materno Infantil.2012; 12(2): 173. CrossRef - Escherichia coli-induced epithelial hyporesponsiveness to secretagogues is associated with altered CFTR localization
Christina L. Ohland, Rebekah DeVinney, Wallace K. MacNaughton
Cellular Microbiology.2012; 14(4): 447. CrossRef - Clinical Implications of Enteroadherent Escherichia coli
Margarita M. P. Arenas-Hernández, Ygnacio Martínez-Laguna, Alfredo G. Torres
Current Gastroenterology Reports.2012; 14(5): 386. CrossRef - Global Fluoroquinolone Resistance Epidemiology and Implictions for Clinical Use
Axel Dalhoff
Interdisciplinary Perspectives on Infectious Diseases.2012; 2012: 1. CrossRef - EnteroaggregativeEscherichia colipathotype: a genetically heterogeneous emerging foodborne enteropathogen
Teresa Estrada-Garcia, Fernando Navarro-Garcia
FEMS Immunology & Medical Microbiology.2012; 66(3): 281. CrossRef