In the present study, to improve the photosynthetic betacarotene
productivity of Dunaliella salina, a blue-red LED
wavelength-shifting system (B-R system) was investigated.
Dunaliella salina under the B-R system showed enhanced
density and beta-carotene productivity compared to D. salina
cultivated under single light-emitting diode light wavelengths
(blue, white, and red light-emitting diode). Additionally, we
developed blue light-adapted D. salina (ALE-D. salina) using
an adaptive laboratory evolution (ALE) approach. In combination
with the B-R system applied to ALE-D. salina (ALE
B-R system), the beta-carotene concentration (33.94 ± 0.52
μM) was enhanced by 19.7% compared to that observed for
the non-ALE-treated wild-type of D. salina (intact D. salina)
under the B-R system (28.34 ± 0.24 μM).