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Isolation of an algicide from a marine bacterium and its effects against the toxic dinoflagellate Alexandrium catenella and other harmful algal bloom species
Yun Sook Kim , Hong-Joo Son , Seong-Yun Jeong
J. Microbiol. 2015;53(8):511-517.   Published online July 31, 2015
DOI: https://doi.org/10.1007/s12275-015-5303-1
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  • 25 Crossref
AbstractAbstract
The aim of this study was to isolate and identify bacteria demonstrating an algicidal effect against Alexandrium catenella and to determine the activity and range of any algicide discovered. The morphological and biochemical attributes of an algicidal bacterium, isolate YS-3, and analysis of its 16S rRNA gene sequence revealed it to be a member of the genus Brachybacterium. This organism, designated Brachybacterium sp. YS-3, showed the greatest effect against A. catenella cells of all bacteria isolated, and is assumed to produce secondary metabolites. When 10% solutions of culture filtrates from this strain were applied to A. catenella cultures, over 90% of cells were killed within 9 h. Bioassay-guided isolation of the algicide involved led to the purification and identification of an active compound. Based on physicochemical and spectroscopic data, including nuclear magnetic resonance and mass analyses, this compound was identified as 1-acetyl-β-carboline. This algicide showed significant activity against A. catenella and a wide range of harmful algal bloom (HAB)-forming species. Taken together, our results suggest that Brachybacterium sp. YS-3 and its algicide represent promising candidates for use in HAB control.

Citations

Citations to this article as recorded by  
  • Characterization of a novel algicidal bacteria Arenibacter sp. strain 6A1 and its application to eliminate harmful algal blooms
    Sha Wu, Jing Tong, Jiahuan Chen, Minchun Chen, Liyan Wang, Shuangfei Li, Zhangli Hu, Huirong Chen
    Frontiers in Marine Science.2024;[Epub]     CrossRef
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    Frontiers in Microbiology.2024;[Epub]     CrossRef
  • Brachybacterium atlanticum sp. nov., a novel marine bacterium isolated from the Atlantic Ocean
    Inês de Castro, Susana Ribeiro, Vanessa Oliveira, Francisco J. R. C. Coelho, Maria de Lurdes Dapkevicius, Eduardo Brito de Azevedo, Joana Barcelos e Ramos
    International Journal of Systematic and Evolutionary Microbiology .2023;[Epub]     CrossRef
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    Luwei Zheng, Hong Lin, Barathan Balaji-Prasath, Yuping Su, Ying Wang, Yi Zheng, Guanglang Yu
    Frontiers in Microbiology.2023;[Epub]     CrossRef
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    Bin Zhang, Ying Yang, Wei He, Wei Liu
    Genomics.2023; 115(2): 110586.     CrossRef
  • Prokaryotic Diversity and Dynamics during Dinoflagellate Bloom Decays in Coastal Tunisian Waters
    Rim Lajnef, Marianne Quéméneur, Moufida Abdennadher, Lamia Dammak Walha, Asma Hamza, Malika Belhassen, Amel Bellaaj Zouari
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    Xi Chen, Dengyu Wang, Yanqun Wang, Pengfei Sun, Shuanghui Ma, Tiantian Chen
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  • Functional role of a novel algicidal compound produced by Pseudoruegeria sp. M32A2M on the harmful algae Alexandrium catenella
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    Journal of Environmental Management.2020; 268: 110687.     CrossRef
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    Water, Air, & Soil Pollution.2019;[Epub]     CrossRef
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    Nils Meyer, Arite Bigalke, Anett Kaulfuß, Georg Pohnert
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  • Photosynthetic inhibition and oxidative stress to the toxic Phaeocystis globosa caused by a diketopiperazine isolated from products of algicidal bacterium metabolism
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    Journal of Microbiology.2016; 54(5): 364.     CrossRef
Isolation and Characterization of a Marine Algicidal Bacterium against the Harmful Raphidophyceae Chattonella marina
Yun Sook Kim , Dae-Sung Lee , Seong-Yun Jeong , Won Jae Lee , Myung-Suk Lee
J. Microbiol. 2009;47(1):9-18.   Published online February 20, 2009
DOI: https://doi.org/10.1007/s12275-008-0141-z
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  • 63 Crossref
AbstractAbstract
A bacterial strain named AB-4 showing algicidal activity against Chattonella marina was isolated from coastal water of Uljin, Republic of Korea. The isolated strain was identified as Bacillus sp. by culture morphology, biochemical reactions, and homology research based on 16S rDNA. The bacterial culture led to the lysis of algal cells, suggesting that the isolated strain produced a latent algal-lytic compound. Amongst changes in algicidal activity by different culture filtrate volumes, the 10% (100 ?/ml) concentration showed the biggest change in algicidal activity; there, estimated algicidal activity was 95%. The swimming movements of Chattonella marina cells were inhibited because of treatment of the bacterial culture; subsequently, Chattonella marina cells became swollen and rounded. With longer exposure time, algal cells were disrupted and cellular components lost their integrity and decomposed. The released algicide(s) were heat-tolerant and stable in pH variations, except pH 3, 4, and 5. Culture filtrate of Bacillus sp. AB-4 was toxic against harmful algae bloom (HAB) species and nontoxic against livefood organisms. Bacillus sp. AB-4 showed comparatively strong activity against Akashiwo sanguinea, Fibriocapsa japonica, Heterosigma akashiwo, and Scrippsiella trochoidea. These results suggest that the algicidal activity of Bacillus sp. AB-4 is potentially useful for controlling outbreaks of Chattonella marina.

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