Journal of Microbiology

1 "ampicillin-resistance"

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Research Support, Non-U.S. Gov't

Serotype Distribution and β-Lactam Resistance in Haemophilus influenzae Isolated from Patients with Respiratory Infections in Korea: Songmee Bae , Jaehoon Lee , Eunah Kim , Jaehwa Lee , Jaeyon Yu , Yeonho Kang; J. Microbiol. 2010;48(1):84-88. Published online March 11, 2010; DOI: https://doi.org/10.1007/s12275-009-0212-9

Abstract: Haemophilus influenzae is a frequent causative bacterial pathogen of respiratory tract infections. Resistance to β-lactam antibiotics has been a significant clinical problem in treatment for H. influenzae respiratory infections. This study describes the serotype, antibiotic resistance and distribution of TEM-1 or ROB-1 β-lactamase in H. influenzae isolates from local private hospitals from 2002 to 2004. Among the 100 H. influenzae respiratory isolates, only 7% were identified as serotypes a, b, e, and f, with the remaining 93% being nontypeable. Resistance to ampicillin, cefaclor, and tetracycline was 57%, 46%, and 16%, respectively. All strains were susceptible to azithromycin and ciprofloxacin, whereas amoxicillin/clavulanate, cefotaxime, and imipenem exhibited reduced susceptibilities of 99%, 99%, and 91%, respectively. All 57 ampicillinresistant strains (minimum inhibitory concentration, MIC≥4 µg/ml) were β-lactamase-positive and possessed the TEM-1 type β-lactamase. One β-lactamase-positive amoxicillin/clavulanate-resistant isolate that was resistant to ampicillin (MIC>128 µg/ml) had the TEM-1 type β-lactamase and not susceptible to cefaclor and cefotaxime. Analysis of penicillin binding protein 3 revealed six residues (Asp-350, Met-377, Ala-502, Asn-526, Val-547, and Asn-569)that were substituted by Asn, Ile, Val, Lys, Ile, and Ser, respectively.

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