Research Support, Non-U.S. Gov't
- Pregnancy - associated human listeriosis: Virulence and genotypic analysis of Listeria monocytogenes from clinical samples
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Dharmendra Kumar Soni , Durg Vijai Singh , Suresh Kumar Dubey
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J. Microbiol. 2015;53(9):653-660. Published online August 1, 2015
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DOI: https://doi.org/10.1007/s12275-015-5243-9
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Abstract
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Listeria monocytogenes, a life-threatening pathogen, poses
severe risk during pregnancy, may cause abortion, fetal death
or neonatal morbidity in terms of septicemia and meningitis.
The present study aimed at characterizing L. monocytogenes
isolated from pregnant women based on serotyping, antibiotic
susceptibility, virulence genes, in vivo pathogenicity test and
ERIC- and REP-PCR fingerprint analyses. The results revealed
that out of 3700 human clinical samples, a total of 30 (0.81%)
isolates [12 (0.80%) from placental bit (1500), 18 (0.81%) from
vaginal swab (2200)] were positive for L. monocytogenes. All
the isolates belonged to serogroup 4b, and were + ve for
virulence genes tested i.e. inlA, inlC, inlJ, plcA, prfA, actA,
hlyA, and iap. Based on the mice inoculation tests, 20 isolates
showed 100% and 4 isolates 60% relative virulence while
6 isolates were non-pathogenic. Moreover, 2 and 10 isolates
were resistant to ciprofloxacin and cefoxitin, respectively,
while the rest susceptible to other antibiotics used in this
study. ERIC- and REP-PCR collectively depicted that the isolates
from placental bit and vaginal swab had distinct PCR
fingerprints except a few isolates with identical patterns. This
study demonstrates prevalence of pathogenic strains mostly
resistant to cefoxitin and/or ciprofloxacin. The results indicate
the importance of isolating and characterizing the pathogen
from human clinical samples as the pre-requisite for accurate
epidemiological investigations.
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Citations
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Journal Article
- Epidemiological Features and Resistance Pattern in Uropathogens Isolated from Chronic Bacterial Prostatitis
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Tommaso Cai , Sandra Mazzoli , Francesca Meacci , Vieri Boddi , Nicola Mondaini , Gianni Malossini , Riccardo Bartoletti
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J. Microbiol. 2011;49(3):448-454. Published online June 30, 2011
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DOI: https://doi.org/10.1007/s12275-011-0391-z
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44
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Abstract
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Chronic bacterial prostatitis (CBP) is, usually, caused by uropathogens, especially gram-negative bacilli, although infection is sometimes due to Gram-positive and atypical microorganisms. A recent increasing in prevalence of Gram-positive strains has been reported. The aim of this study was to explore the epidemiological features and resistance rates in uropathogens isolated from CBP outpatients in last 10 years. All consecutive outpatients with demonstrated CBP attending a single Sexually Transmitted Disease centre from January 1997 and December 2008, were enrolled and underwent microbiological cultures in first void early morning urine, midstream urine, expressed prostatic secretion, and post prostate massage urine. Prevalence of different bacterial strains was stratified in four different periods: 1997-1999, 2000-2002, 2003-2005, 2006-2008. Any changes observed in epidemiological features and resistance rates in uropathogens over the whole study period have been analyzed. The present study has been planned, thus, as in vitro study. From 6,221 patients, 4,601 Gram-positive and 1,620 Gram-negative bacterial strains have been isolated. Enterococcus faecalis and Escherichia coli strains are the first and second frequent pathogens found, respectively. Significant differences between E. faecalis prevalence in the 1997-1999 and 2006-2008 periods were found. E. coli showed a significant difference between prevalence in 1997-1999 and 2006-2008 periods. Gram-positive organisms showed a decreasing of susceptibility to ciprofloxacin as well as Gram-negative strains, while a good susceptibility to the levofloxacin was evidenced. E. faecalis prevalence seemed to be raised in 2006-2008 periods. Moreover, a decreasing of activity of ciprofloxacin and a good activity profile of levofloxacin have been reported.
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Research Support, Non-U.S. Gov't
- Diversity of Endophytic Enterobacteria Associated with Different Host Plants
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Adalgisa Ribeiro Torres , Welington Luiz Araujo , Luciana Cursino , Mariangela Hungria , Fabio Plotegher , Fabio Luis Mostasso , Joao Lucio Azevedo
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J. Microbiol. 2008;46(4):373-379. Published online August 31, 2008
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DOI: https://doi.org/10.1007/s12275-007-0165-9
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Abstract
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Fifty-three endophytic enterobacteria isolates from citrus, cocoa, eucalyptus, soybean, and sugar cane were evaluated for susceptibility to the antibiotics ampicillin and kanamycin, and cellulase production. Susceptibility was found on both tested antibiotics. However, in the case of ampicillin susceptibility changed according to the host plant, while all isolates were susceptible to kanamycin. Cellulase production also changed according to host plants. The diversity of these isolates was estimated by employing BOX-PCR genomic fingerprints and 16S rDNA sequencing. In total, twenty-three distinct operational taxonomic units (OTUs) were identified by employing a criterion of 60% fingerprint similarity as a surrogate for an OTU. The 23 OTUs belong to the Pantoea and Enterobacter genera, while their high diversity could be an indication of paraphyletic classification. Isolates representing nine different OTUs belong to Pantoea agglomerans, P. ananatis, P. stewartii, Enterobacter sp., and E. homaechei. The results of this study suggest that plant species may select endophytic bacterial genotypes. It has also become apparent that a review of the Pantoea/Enterobacter genera may be necessary.
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- Prevalence and Antibiotic Susceptibility of Vancomycin-Resistant Enterococci in Chicken Intestines and Fecal Samples from Healthy Young Children and Intensive Care Unit Patients
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Shin Moo Kim , Eun Sook Shim , Chi Nam Seong
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J. Microbiol. 2001;39(2):116-120.
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Abstract
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The prevalence, resistance genotype and antibiotic susceptibility of vancomycin-resistant enterococci (VRE) were determined. Prevalence of VRE in chickens, healthy children and intensive care unit (ICU) patients was 43.0%, 12.7% and 24.1%, respectively. Forty out of 56 isolates from chicken intestines were identified as Enterococcus faecium, and 12 were E. faecalis. All the isolates contained the vanA gene. Nine out of 13 VRE isolates from patients and two out of 21 from healthy young children were identified as E. faecium. The resistance types of E. faecium, E. gallinarium and E. casseliflavus were VanA, VanC1, and VanC2, respectively. The mimimum inhibitory concentrations (MICs) of E. faecium, E. gallinarium, and E. casseliflavus to vancomycin were 512, 8 and 4 g/ml, respectively. Specifically, E. faecium isolates were resistant to most of antibiotics except ampicillin and gentamicin. This is the first report of high VanA type VRE prevalence in nonhospitalized young children in Korea.