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Antiviral activity of Schizonepeta tenuifolia Briquet against noroviruses via induction of antiviral interferons
Yee Ching Ng , Ye Won Kim , Jeong-Su Lee , Sung Joon Lee , Moon Jung Song
J. Microbiol. 2018;56(9):683-689.   Published online August 23, 2018
DOI: https://doi.org/10.1007/s12275-018-8228-7
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  • 16 Crossref
AbstractAbstract
Human noroviruses are the causative agents of non-bacterial gastroenteritis worldwide. The rapid onset and resolution of disease symptoms suggest that innate immune responses are critical for controlling norovirus infection; however, no effective antivirals are yet available. The present study was conducted to examine the antiviral activities of Schizonepeta tenuifolia Briquet extract (STE) against noroviruses. Treatment of human norovirus replicon-bearing HG23 cells with STE at 5 and 10 mg/ml concentrations resulted in the reduction in the viral RNA levels by 77.2% and 85.9%, respectively. STE had no cytotoxic effects on HG23 cells. Treatment of RAW 264.7 cells infected with murine norovirus 1 (MNV-1), a surrogate virus of human noroviruses, with STE at 10 and 20 μg/ml concentrations resulted in the reduction of viral replication by 58.5% and 84.9%, respectively. STE treatment induced the expression of mRNAs for type I and type II interferons in HG23 cells and upregulated the transcription of interferon-β in infected RAW 264.7 cells via increased phosphorylation of interferon regulatory factor 3, a critical transcription regulator for type I interferon production. These
results
suggest that STE inhibits norovirus replication through the induction of antiviral interferon production during virus replication and may serve as a candidate antiviral substance for treatment against noroviruses.

Citations

Citations to this article as recorded by  
  • Comparison of Three Different Extraction Methods on Schizonepeta tenuifolia (Benth.) Briq Essential Oil: Chemical Constituents and in Vitro and in Silico Biological Activities
    Linhong He, Lijuan Zhang, Xi Bai
    Chemistry & Biodiversity.2024;[Epub]     CrossRef
  • Systems Pharmacological Approach to Identification of Schizonepeta teunifolia Extract via Active Ingredients Analysis and Cytotoxicity Effect on A549 Cell Lines
    Ga Ram Yang, Ji Eun Choo, Youn Sook Kim, Won Gun Ahn
    Korean Journal of Acupuncture.2024; 41(1): 7.     CrossRef
  • Chemical Characteristics and Comparison of Schizonepetae Herba and Schizonepetae Herba Carbonisata by Combination of GC–MS and UHPLC–MS Strategies
    Ding Xiaoying, Shenghui Hao, Hengyang Li, Tao Wang, Wenjie Li, Shengjiang Guan, Yuguang Zheng, Long Guo, Dan Zhang
    Journal of AOAC International.2023; 106(4): 970.     CrossRef
  • Efficacy analysis and research progress of complementary and alternative medicines in the adjuvant treatment of COVID-19
    Jaung-Geng Lin, Guan-Jhong Huang, Yi-Chang Su
    Journal of Biomedical Science.2023;[Epub]     CrossRef
  • Colorimetric Parameters Correlated with the Variation in the Marker Constituent Contents During the Stir-fry Processing of Schizonepetae Spica
    Xindan Liu, Ying Zhang, Menghua Wu, Zhiguo Ma, Hui Cao
    Food Analytical Methods.2022; 15(5): 1300.     CrossRef
  • Discrimination and Characterization of the Volatile Organic Compounds in Schizonepetae Spica from Six Regions of China Using HS-GC-IMS and HS-SPME-GC-MS
    Chao Li, Huiying Wan, Xinlong Wu, Jiaxin Yin, Limin Zhu, Hanjiang Chen, Xinbo Song, Lifeng Han, Wenzhi Yang, Heshui Yu, Zheng Li
    Molecules.2022; 27(14): 4393.     CrossRef
  • Review on Chemical Constituents of Schizonepeta tenuifolia Briq. and Their Pharmacological Effects
    Xueying Zhao, Mingwei Zhou
    Molecules.2022; 27(16): 5249.     CrossRef
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    Gang Tian, Mengting Gao, Chao Li, Fei Shen, Feng Zhu, Xin Li, Yifei Wang, Beihua Bao, Li Zhang, Zongli Huo, Weifeng Yao
    Journal of Chromatographic Science.2022; 61(1): 38.     CrossRef
  • A review of the botany, traditional uses, phytochemistry and pharmacology of Nepeta tenuifolia Briq.
    Mingqiu Shan, Yulan Jiang, Yuanyuan Fu, Yongyi Zhou, Zhihui Lu, Sheng Yu, Hui Yan, Chanchan Liu, Peidong Chen, Beihua Bao, Li Zhang, Qinan Wu
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  • Spectrum–effect relationship between GC‐QTOF‐MS fingerprint and antioxidant, anti‐inflammatory activities of Schizonepeta tenuifolia essential oil
    Xi Bai, Liu Liu, Junping Zhang, Li Chen, Tao Wu, Haji Akber Aisa, Maitinuer Maiwulanjiang
    Biomedical Chromatography.2021;[Epub]     CrossRef
  • Interferon responses to norovirus infections: current and future perspectives
    Aminu S. Jahun, Ian G. Goodfellow
    Journal of General Virology .2021;[Epub]     CrossRef
  • Comparisons of the anti-inflammatory, antiviral, and hemostatic activities and chemical profiles of raw and charred Schizonepetae Spica
    Xindan Liu, Zihan Huang, Jinju Zhang, Yu Zhou, Ying Zhang, Menghua Wu, Zhiguo Ma, Hui Cao
    Journal of Ethnopharmacology.2021; 278: 114275.     CrossRef
  • The Potential Transformation Mechanisms of the Marker Components of Schizonepetae Spica and Its Charred Product
    Xindan Liu, Ying Zhang, Menghua Wu, Zhiguo Ma, Hui Cao
    Molecules.2020; 25(16): 3749.     CrossRef
  • Color discrimination and gas chromatography-mass spectrometry fingerprint based on chemometrics analysis for the quality evaluation of Schizonepetae Spica
    Xindan Liu, Ying Zhang, Menghua Wu, Zhiguo Ma, Hui Cao, Joseph Banoub
    PLOS ONE.2020; 15(1): e0227235.     CrossRef
  • Glycolysis Is an Intrinsic Factor for Optimal Replication of a Norovirus
    Karla D. Passalacqua, Jia Lu, Ian Goodfellow, Abimbola O. Kolawole, Jacob R. Arche, Robert J. Maddox, Kelly E. Carnahan, Mary X. D. O’Riordan, Christiane E. Wobus, Mary K. Estes
    mBio.2019;[Epub]     CrossRef
Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo
Prasanna Weeratunga , Thilina U. B. Herath , Tae-Hwan Kim , Hyun-Cheol Lee , Jae-Hoon Kim , Byeong-Hoon Lee , Eun-Seo Lee , Kiramage Chathuranga , W. A. Gayan Chathuranga , Chul-Su Yang , Jin Yeul Ma , Jong-Soo Lee
J. Microbiol. 2017;55(11):909-917.   Published online October 27, 2017
DOI: https://doi.org/10.1007/s12275-017-7392-5
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  • 15 Crossref
AbstractAbstract
Dense granule protein-7 (GRA-7) is an excretory protein of Toxoplasma gondii. It is a potential serodiagnostic marker and vaccine candidate for toxoplasmosis. Previous reports demonstrated that GRA-7 induces innate immune responses in macrophages by interacting with TRAF6 via the MyD88- dependent pathway. In the present study, we evaluated the antiviral activity and induction of an antiviral state by GRA-7 both in vitro and in vivo. It was observed that GRA-7 markedly reduced the replication of vesicular stomatitis virus (VSVGFP), influenza A virus (PR8-GFP), coxsackievirus (H3- GFP), herpes simplex virus (HSV-GFP), and adenovirus-GFP in epithelial (HEK293T/HeLa) and immune (RAW264.7) cells. These antiviral activities of GRA-7 were attributed to the induction of type I interferon (IFN) signaling, resulting in the secretion of IFNs and pro-inflammatory cytokines. Additionally, in BALB/c mice, intranasal administration of GRA-7 prevented lethal infection by influenza A virus (H1N1) and exhibited prophylactic effects against respiratory syncytial virus (RSV-GFP). Collectively, these results suggested that GRA-7 exhibits immunostimulatory and broad spectrum antiviral activities via type I IFN signaling. Thus, GRA-7 can be potentially used as a vaccine adjuvant or as a candidate drug with prophylactic potential against viruses.

Citations

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  • Effect of SARS-CoV-2 and Toxoplasma gondii co-infection on IFN-γ and TNF-α expression and its impact on disease severity
    Magda S.A. Abdeltawab, Mohamed Fateen, Shimaa Saad El-Din, Riem M. Elmessiery, Osama Mohammady Mohamed, Khaled Marzouk Sadek, Engy Medhat, Alshaimaa M.R. Hamed
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    Microbial Pathogenesis.2021; 158: 105016.     CrossRef
  • Role of interferon gamma in SARS-CoV-2-positive patients with parasitic infections
    Enas Fakhry Abdel-Hamed, Mohamed N. Ibrahim, Nahed E. Mostafa, Howayda S. F. Moawad, Nahla E. Elgammal, Ehab M. Darwiesh, Dina S. El-rafey, Nissreen E. ElBadawy, Emad Ali Al-Khoufi, Salwa I. Hindawi
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Research Support, Non-U.S. Gov't
Cyclic Dipeptides from Lactic Acid Bacteria Inhibit Proliferation of the Influenza A Virus
Min-Kyu Kwak , Rui Liu , Jun-Oh Kwon , Min-Kyu Kim , Andrew HyoungJin Kim , Sa-Ouk Kang
J. Microbiol. 2013;51(6):836-843.   Published online December 19, 2013
DOI: https://doi.org/10.1007/s12275-013-3521-y
  • 39 View
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  • 51 Crossref
AbstractAbstract
We isolated Lactobacillus plantarum LBP-K10 from the traditional Korean fermented food kimchi. When organic acids were removed, the culture filtrate of this isolate showed high antiviral activity (measured using a plaque-forming assay) against the influenza A (H3N2) virus. Two fractions that were active against influenza A virus were purified from the culture filtrate using a C18 column with high-performance liquid chromatography. These active fractions were crystallized and identified to be the cyclic dipeptides cis-cyclo (L-Leu-L-Pro) and cis-cyclo(L-Phe-L-Pro) using gas chromatography-mass spectrometry; this identification was confirmed by X-ray crystallography. These cyclic dipeptides were identified in the culture filtrate of other lactic acid bacteria, including Lactobacillus spp., Leuconostoc spp., Weissella spp., and Lactococcus lactis.

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Measurement of Antiviral Activities Using Recombinant Human Cytomegalovirus
Byung-Hak Song , Gyu-Cheol Lee , Chan-Hee Lee
J. Microbiol. 2000;38(4):255-259.
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AbstractAbstract
For rapid and sensitive measurement of antiviral activities, application of a recombinant virus containing firefly luciferase gene was attempted. Recombinant human cytomegalovirus (HCMV) containing luciferase gene driven by HCMV late gene pp28 promoter (HCMV/pp28-luc) was used to test the antiviral activities of three known compounds and the result was compared with results from the conventional plaque assay for measuring the production of infectious viruses. When human fibroblast cells were infected with HCMV/pp28-luc, luciferase activity was observed at 2 days after infection and reached maximum at 6 days after infection, whereas the production of infectious virus was maximal at 4 days after infection. The antiviral activities of ganciclovir, acyclovir, and papaverine were measured in HFF cells infected with HCMV/pp28-luc and the luciferase activity was compared with the infectious virus titers. Luciferase activity decreased as the concentration of ganciclovir or papaverine increased, while there was a slight decrease in luciferase activity with acyclovir. The level of the decrease in luciferase activity was comparable to the level of decrease in the production of infectious virus. Therefore, the antiviral assay using recombinant virus HCMV/pp28-luc resulted in sensitivity similar to the conventional plaque assay with a significant reduction in assay time.

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