Research Support, Non-U.S. Gov't
- Antiviral effects of Lactobacillus ruminis SPM0211 and Bifidobacterium longum SPM1205 and SPM1206 on rotavirus-infected Caco-2 cells and a neonatal mouse model
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Joo Yeon Kang , Do Kyung Lee , Nam Joo Ha , Hea Soon Shin
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J. Microbiol. 2015;53(11):796-803. Published online October 28, 2015
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DOI: https://doi.org/10.1007/s12275-015-5302-2
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Abstract
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Rotavirus is worldwide cause of severe gastroenteritis including
severe diarrhea and fatal dehydration in infants
and young children. There is an available vaccination program
for preventing rotavirus infection, but it has limits
and restrictions. Probiotics therapy could be an alternative
method
of antiviral prevention and modulation against rotavirus
infection. In this study, we screened the antiviral activity
of probiotic bacteria such as 3 Lactobacillus spp. and
14 Bifidobacterium spp. isolated from young Korean. Three
of the bacteria, Lactobacillus ruminis SPM0211, Bifidobacterium
longum SPM1205, and SPM1206, inhibited human
strain Wa rotavirus infection in Caco-2 cells. Furthermore,
these bacterial strains inhibited rotavirus replication in a
rotavirus-infected neonatal mouse model. To clarify the mechanism
of inhibition, we investigated gene expression of
Interferon (IFN)-signaling components and IFN-inducible
antiviral effectors. All 3 probiotics increased IFN-α and IFN-
β levels compared with the control. Gene expression of IFNsignaling
components and IFN-inducible antiviral effectors
also increased. Overall, these results indicate that L. ruminis
SPM0211, B. longum SPM1205 and 1206 efficiently inhibit
rotavirus replication in vitro and in vivo. Especially, the antiviral
effect of Lactobacillus ruminis SPM0211 is worthy of
notice. This is the first report of L. ruminis with antiviral activity.
Anti-rotaviral effects of the 3 probiotics are likely due
to their modulation of the immune response through promoting
type I IFNs, which are key regulators in IFN signaling
pathway.
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