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- Phosphorylation of tegument protein pp28 contributes to trafficking to the assembly compartment in human cytomegalovirus infection
- Jun-Young Seo , Jin Ah Heo , William J. Britt
- J. Microbiol. 2020;58(7):624-631. Published online June 27, 2020
- DOI: https://doi.org/10.1007/s12275-020-0263-5
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- 5 Web of Science
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Abstract
- Human cytomegalovirus (HCMV) UL99 encodes a late tegument protein pp28 that is essential for envelopment and production of infectious virus. This protein is localized to the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) in transfected cells but it localizes to the cytoplasmic assembly compartment (AC) in HCMV-infected cells. Trafficking of pp28 to the AC is required for the assembly of infectious virus. The N-terminal domain (aa 1-61) of pp28 is sufficient for trafficking and function of the wild type protein during viral infection. However, residues required for authentic pp28 trafficking with the exception of the acidic cluster in the N-terminal domain of pp28 remain undefined. Monitoring protein migration on SDS-PAGE, we found that pp28 is phosphorylated in the virus-infected cells and dephosphorylated in the viral particles. By generating substitution mutants of pp28, we showed that three serine residues (aa 41–43) and a tyrosine residue (aa 34) account for its phosphorylation. The mutant forms of pp28 were localized to the plasma membrane as well as the ERGIC in transfected cells. Likewise, these mutant proteins were localized to the plasma membrane as well as the AC in virus-infected cells. These results suggested that phosphorylation of pp28 contributes to its intracellular trafficking and efficient viral assembly and incorporation.
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Citations
Citations to this article as recorded by
- Exploring the genetic associations and causal relationships between antibody responses, immune cells, and various types of breast cancer
Yang Yang, Jiayi Chen, Fuhong Gong, Jingge Miao, Mengping Lin, Ruimin Liu, Chenxi Wang, Fei Ge, Wenlin Chen
Scientific Reports.2024;[Epub] CrossRef - Human cytomegalovirus induces significant structural and functional changes in terminally differentiated human cortical neurons
Jacob W. Adelman, Suzette Rosas-Rogers, Megan L. Schumacher, Rebekah L. Mokry, Scott S. Terhune, Allison D. Ebert, Thomas Shenk
mBio.2023;[Epub] CrossRef - Insights into the Transcriptome of Human Cytomegalovirus: A Comprehensive Review
Janine Zeng, Di Cao, Shaomin Yang, Dabbu Kumar Jaijyan, Xiaolian Liu, Songbin Wu, Ruth Cruz-Cosme, Qiyi Tang, Hua Zhu
Viruses.2023; 15(8): 1703. CrossRef - Features and Functions of the Conserved Herpesvirus Tegument Protein UL11 and Its Binding Partners
Linjiang Yang, Mingshu Wang, Anchun Cheng, Qiao Yang, Ying Wu, Juan Huang, Bin Tian, Renyong Jia, Mafeng Liu, Dekang Zhu, Shun Chen, Xinxin Zhao, Shaqiu Zhang, Xumin Ou, Sai Mao, Qun Gao, Di Sun
Frontiers in Microbiology.2022;[Epub] CrossRef - The human cytomegalovirus decathlon: Ten critical replication events provide opportunities for restriction
Declan L. Turner, Rommel A. Mathias
Frontiers in Cell and Developmental Biology.2022;[Epub] CrossRef
- Exploring the genetic associations and causal relationships between antibody responses, immune cells, and various types of breast cancer
- Growth of cyanobacterial soil crusts during diurnal freeze-thaw cycles
- Steven K. Schmidt , Lara Vimercati
- J. Microbiol. 2019;57(4):243-251. Published online February 5, 2019
- DOI: https://doi.org/10.1007/s12275-019-8359-5
- 48 View
- 0 Download
- 16 Web of Science
- 13 Crossref
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Abstract
- Various Nostoc spp. and related cyanobacteria are able to survive extreme temperatures and are among the most successful colonists of high-elevation sites being exposed due to glacial retreat. It is unclear, however, if cyanobacteria can grow during the extreme freeze-thaw cycles that occur on a yearround basis at high-elevation, peri-glacial sites or if they only grow during the rare periods when freeze-thaw cycles do not occur. We conducted several experiments to determine if cyanobacteria that form biological soil crusts (BSCs) at highelevation sites (> 5,000 m.a.s.l.) in the Andes can grow during diurnal freeze-thaw cycles on a par with those that occur in the field. Here we show that a soil crust that had been frozen at -20°C for five years was able to increase from 40% to 100% soil coverage during a 45-day incubation during which the soil temperature cycled between -12°C and 26°C every day. In a second, experiment an undeveloped soil with no visible BSCs showed a statistically significant shift in the bacterial community from one containing few cyanobacterial sequences (8% of sequences) to one dominated (27%) by Nostoc, Microcoleus, and Leptolyngbya phylotypes during a 77-day incubation with daily freeze-thaw cycles. In addition, counts of spherical Nostoc-like colonies increased significantly on the soil surface during the experiment, especially in microcosms receiving phosphorus. Taken together these results show that freeze-thaw cycles alone do not limit the growth of BSCs in high-elevation soils, and provide new insight into how life is able to thrive in one of the most extreme terrestrial environments on Earth.
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Citations
Citations to this article as recorded by- Controlling enhanced surface runoff components as a result of a freezing-thawing cycle by inoculating soil bacteria and cyanobacteria
Sudabeh Gharemahmudli, Seyed Hamidreza Sadeghi, Ali Najafinejad, Behrouz Zarei Darki, Ali Mohammadian Behbahani, Hossein Kheirfam
Soil and Tillage Research.2024; 237: 105989. CrossRef - Cyanobacterial Biocrust on Biomineralized Soil Mitigates Freeze–Thaw Effects and Preserves Structure and Ecological Functions
Keiichi Kimura, Toshiya Okuro
Microbial Ecology.2024;[Epub] CrossRef - Cyanobacterial biocrust alters soil physical properties reducing soil erosion and aerosol production
Amir Karimi, Arezoo Tahmourespour, Mehran Hoodaji
Brazilian Journal of Microbiology.2024; 55(3): 2453. CrossRef - Bacterial and plant community successional pathways in glacier forefields of the Western Himalaya
Adam T. Ruka, Kateřina Čapková, Klára Řeháková, Roey Angel, Alica Chroňáková, Martin Kopecký, Martin Macek, Miroslav Dvorský, Jiří Doležal
European Journal of Soil Biology.2023; 119: 103565. CrossRef - Biocrusts from Iceland and Svalbard: Does microbial community composition differ substantially?
Ekaterina Pushkareva, Josef Elster, Andreas Holzinger, Sarina Niedzwiedz, Burkhard Becker
Frontiers in Microbiology.2022;[Epub] CrossRef - Effect of snow cover on water content, carbon and nutrient availability, and microbial biomass in complexes of biological soil crusts and subcrust soil in the desert
Rong Hui, Ruiming Zhao, Lichao Liu, Xinrong Li
Geoderma.2022; 406: 115505. CrossRef - Reduction in soil loss caused by a freeze-thaw cycle through inoculation of endemic soil microorganisms
Seyed Hamidreza Sadeghi, Ali Najafinejad, Sudabeh Gharemahmudli, Behrouz Zarei Darki, Ali Mohammadian Behbahani, Hossein Kheirfam
Applied Soil Ecology.2021; 157: 103770. CrossRef - Diversity of microbial phototrophs and heterotrophs in Icelandic biocrusts and their role in phosphorus-rich Andosols
Ekaterina Pushkareva, Karen Baumann, Anh Tu Van, Tatiana Mikhailyuk, Christel Baum, Katarzyna Hrynkiewicz, Eduard Demchenko, Dominika Thiem, Tina Köpcke, Ulf Karsten, Peter Leinweber
Geoderma.2021; 386: 114905. CrossRef - Multiple‐trophic patterns of primary succession following retreat of a high‐elevation glacier
Weiming Hu, Steven K. Schmidt, Pacifica Sommers, John L. Darcy, Dorota L. Porazinska
Ecosphere.2021;[Epub] CrossRef - Freeze-thaw cycles change the physiological sensitivity of Syntrichia caninervis to snow cover
Benfeng Yin, Jiwen Li, Qing Zhang, Nan Wu, Jing Zhang, Xiaoying Rong, Ye Tao, Yongxin Zang, Yonggang Li, Xiaobing Zhou, Yuanming Zhang
Journal of Plant Physiology.2021; 266: 153528. CrossRef - Cyanobacteria in early soil development of deglaciated forefields: Dominance of non-heterocytous filamentous cyanobacteria and phosphorus limitation of N-fixing Nostocales
Joseph E. Knelman, Steve K. Schmidt, Emily B. Graham
Soil Biology and Biochemistry.2021; 154: 108127. CrossRef - Snowpack shifts cyanobacterial community in biological soil crusts
Bingchang Zhang, Yongqing Zhang, Xiaobing Zhou, Xiangzhen Li, Yuanming Zhang
Journal of Arid Land.2021; 13(3): 239. CrossRef - The Role of Cyanobacterial External Layers in Mass Transfer: Evidence from Temperature Shock Experiments by Noninvasive Microtest Technology
Yan Xiao, Lingxin Liu, Zhe Li, Yuran Cheng
Microorganisms.2020; 8(6): 861. CrossRef
- Controlling enhanced surface runoff components as a result of a freezing-thawing cycle by inoculating soil bacteria and cyanobacteria
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