Journal of Microbiology

Journal Articles

Syntaxin17 Restores Lysosomal Function and Inhibits Pyroptosis Caused by Acinetobacter baumannii: Zhiyuan An, Wenyi Ding; J. Microbiol. 2024;62(4):315-325. Published online March 7, 2024; DOI: https://doi.org/10.1007/s12275-024-00109-0

56 View
0 Download

Abstract: Acinetobacter baumannii (A. baumannii) causes autophagy flux disorder by degrading STX17, resulting in a serious inflammatory response. It remains unclear whether STX17 can alter the inflammatory response process by controlling autolysosome function. This study aimed to explore the role of STX17 in the regulation of pyroptosis induced by A. baumannii. Our findings indicate that overexpression of STX17 enhances autophagosome degradation, increases LAMP1 expression, reduces Cathepsin B release, and improves lysosomal function. Conversely, knockdown of STX17 suppresses autophagosome degradation, reduces LAMP1 expression, augments Cathepsin B release, and accelerates lysosomal dysfunction. In instances of A. baumannii infection, overexpression of STX17 was found to improve lysosomal function and reduce the expression of mature of GSDMD and IL-1β, along with the release of LDH, thus inhibiting pyroptosis caused by A. baumannii. Conversely, knockdown of STX17 led to increased lysosomal dysfunction and further enhanced the expression of mature of GSDMD and IL-1β, and increased the release of LDH, exacerbating pyroptosis induced by A. baumannii. These findings suggest that STX17 regulates pyroptosis induced by A. baumannii by modulating lysosomal function.

A Method for Physical Analysis of Recombination Intermediates in Saccharomyces cerevisiae: Kiwon Rhee , Hyungseok Choi , Keun P. Kim , Jeong H. Joo; J. Microbiol. 2023;61(11):939-951. Published online December 11, 2023; DOI: https://doi.org/10.1007/s12275-023-00094-w

47 View
0 Download

Abstract: Meiosis is a process through which diploid cells divide into haploid cells, thus promoting genetic diversity. This diversity arises from the formation of genetic crossovers (COs) that repair DNA double-strand breaks (DSBs), through homologous recombination (HR). Deficiencies in HR can lead to chromosomal abnormality resulting from chromosomal nondisjunction, and genetic disorders. Therefore, investigating the mechanisms underlying effective HR is crucial for reducing genome instability. Budding yeast serves as an ideal model for studying HR mechanisms due to its amenability to gene modifications and the ease of inducing synchronized meiosis to yield four spores. During meiosis, at the DNA level, programmed DSBs are repaired as COs or non-crossovers (NCOs) through structural alterations in the nascent D-loop, involving single-end invasions (SEIs) and double-Holliday junctions (dHJs). This repair occurs using homologous templates rather than sister templates. This protocol, using Southern blotting, allows for the analysis and monitoring of changes in DNA structures in the recombination process. One-dimensional (1D) gel electrophoresis is employed to detect DSBs, COs, and NCOs, while twodimensional (2D) gel electrophoresis is utilized to identify joint molecules (JMs). Therefore, physical analysis is considered the most effective method for investigating the HR mechanism. Our protocol provides more comprehensive information than previous reports by introducing conditions for obtaining a greater number of cells from synchronized yeast and a method that can analyze not only meiotic/mitotic recombination but also mitotic replication.

Yeast polyubiquitin unit regulates synaptonemal complex formation and recombination during meiosis: Min-Kyung Jo , Kiwon Rhee , Keun Pil Kim , Soogil Hong; J. Microbiol. 2022;60(7):705-714. Published online July 4, 2022; DOI: https://doi.org/10.1007/s12275-022-2204-y

57 View
0 Download
1 Web of Science
1 Crossref

Abstract

Ubiquitin is highly conserved in most eukaryotes and involved in diverse physiological processes, including cell division, protein quality control, and protein degradation mediated by the ubiquitin-proteasome system after heat shock, glucose-starvation, and oxidative stress. However, the role of the ubiquitin gene UBI4, which contains five consecutive head-to-tail ubiquitin repeats, in meiosis has not been investigated. In this study, we show that the Saccharomyces cerevisiae polyubiquitin precursor gene, UBI4, is required to promote synaptonemal complex (SC) formation and suppress excess doublestrand break formation. Moreover, the proportion of Zip1 polycomplexes, which indicate abnormal SC formation, in cells with a mutation in UBI4 (i.e., ubi4Δ cells) is higher than that of wild-type cells, implying that the UBI4 plays an important role in the early meiotic prophase I. Interestingly, although ubi4Δ cells rarely form full-length SCs in the pachytene stage of prophase I, the Zip3 foci are still seen, as in wild-type cells. Moreover, ubi4Δ cells proficiently form crossover and noncrossover products with a slight delay compared to wild-type cells, suggesting that UBI4 is dispensable in SCcoupled recombination. Our findings demonstrate that UBI4 exhibits dual functions that are associated with both positive and negative roles in SC formation and recombination during meiosis.

Citations

Citations to this article as recorded by

The deubiquitinase Usp7 in Drosophila melanogaster is required for synaptonemal complex maintenance
Cathleen M. Lake, Jennifer Gardner, Salam Briggs, Zulin Yu, Grace McKown, R. Scott Hawley
Proceedings of the National Academy of Sciences.2024;[Epub] CrossRef

Weigela florida inhibits the expression of inflammatory mediators induced by Pseudomonas aeruginosa and Staphylococcus aureus infection: Hyo Bin Kim , Soomin Cho , Yeji Lee , Weihui Wu , Un-Hwan Ha; J. Microbiol. 2022;60(6):649-656. Published online April 30, 2022; DOI: https://doi.org/10.1007/s12275-022-1638-6

48 View
0 Download
3 Web of Science
4 Crossref

Abstract

Inflammatory responses involve the action of inflammatory mediators that are necessary for the clearance of invading bacterial pathogens. However, excessive production of inflammatory mediators can damage tissues, thereby impairing bacterial clearance. Here, we examined the effects of Weigela florida on the expression of inflammatory cytokines induced by Pseudomonas aeruginosa or Staphylococcus aureus infection in macrophages. The results showed that pre-treatment with W. florida markedly downregulated the bacterial infectionmediated expression of cytokines. Additionally, post-treatment also triggered anti-inflammatory effects in cells infected with S. aureus to a greater extent than in those infected with P. aeruginosa. Bacterial infection activated inflammation-associated AKT (Thr308 and Ser473)/NF-κB and MAPK (p38, JNK, and ERK) signaling pathways, whereas W. florida treatment typically inhibited the phosphorylation of AKT/NF‐κB and p38/JNK, supporting the anti‐inflammatory effects of W. florida. The present results suggest that W. florida decreases the infection-mediated expression of inflammatory mediators by inhibiting the AKT/NF-κB and MAPK signaling pathways, implying that it may have potential use as an inhibitory agent of excessive inflammatory responses.

Citations

Citations to this article as recorded by

Multifunctional fluorescence probe for simultaneous detection of viscosity, polarity, and ONOO− and its bioimaging in vitro and vivo
Yuan-Yuan Li, Jia-Ling Hu, Ji-Rou Wu, Yi-Ru Wang, Ai-Hong Zhang, Yu-Wei Tan, Ya-Jing Shang, Ting Liang, Min Li, Ya-Li Meng, Yan-Fei Kang
Biosensors and Bioelectronics.2024; 254: 116233. CrossRef
Polymicrobial interactions influence Mycobacterium abscessus co-existence and biofilm forming capabilities
Nishant Nandanwar, Geoffery Gu, Joy E. Gibson, Michael N. Neely
Frontiers in Microbiology.2024;[Epub] CrossRef
Tissue damage alleviation and mucin inhibition by P5 in a respiratory infection mouse model with multidrug-resistant Acinetobacter baumannii
Jun Hee Oh, Jonggwan Park, Hee Kyoung Kang, Hee Joo Park, Yoonkyung Park
Biomedicine & Pharmacotherapy.2024; 181: 117724. CrossRef
Spatiotemporal Deep-Learning-Based Algal Bloom Prediction for Lake Okeechobee Using Multisource Data Fusion
Yufei Tang, Yingqi Feng, Sasha Fung, Veronica Ruiz Xomchuk, Mingshun Jiang, Tim Moore, Jordon Beckler
IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing.2022; 15: 8318. CrossRef

The inner membrane protein LapB is required for adaptation to cold stress in an LpxC-independent manner: Han Byeol Lee , Si Hyoung Park , Chang-Ro Lee; J. Microbiol. 2021;59(7):666-674. Published online May 15, 2021; DOI: https://doi.org/10.1007/s12275-021-1130-8

48 View
0 Download
10 Web of Science
9 Crossref

Abstract

The inner membrane protein lipopolysaccharide assembly protein B (LapB) is an adaptor protein that activates the proteolysis of LpxC by an essential inner membrane metalloprotease, FtsH, leading to a decrease in the level of lipopolysaccharide in the membrane. In this study, we revealed the mechanism by which the essential inner membrane protein YejM regulates LapB and analyzed the role of the transmembrane domain of LapB in Escherichia coli. The transmembrane domain of YejM genetically and physically interacted with LapB and inhibited its function, which led to the accumulation of LpxC. The transmembrane domain of LapB was indispensable for both its physical interaction with YejM and its regulation of LpxC proteolysis. Notably, we found that the lapB mutant exhibited strong cold sensitivity and this phenotype was not associated with increased accumulation of LpxC. The transmembrane domain of LapB was also required for its role in adaptation to cold stress. Taken together, these
results
showed that LapB plays an important role in both the regulation of LpxC level, which is controlled by its interaction with the transmembrane domain of YejM, and adaptation to cold stress, which is independent of LpxC.

Citations

Citations to this article as recorded by

PhoPQ-mediated lipopolysaccharide modification governs intrinsic resistance to tetracycline and glycylcycline antibiotics in Escherichia coli
Byoung Jun Choi, Umji Choi, Dae-Beom Ryu, Chang-Ro Lee, Mehrad Hamidian, You-Hee Cho
mSystems.2024;[Epub] CrossRef
Lytic transglycosylase repertoire diversity enables intrinsic antibiotic resistance and daughter cell separation in Escherichia coli under acidic stress
Ji Eun Son, Si Hyoung Park, Umji Choi, Chang-Ro Lee, Laurent Poirel
Antimicrobial Agents and Chemotherapy.2024;[Epub] CrossRef
Trans-cinnamaldehyde inhibits Escherichia coli growth by regulating lipopolysaccharide accumulation
Huanling Xing, Xiaomin Liu, Jianhao Lin, Mingfei Sun, Junyi Huang, Xinghai Li, Yanqun Li, Shining Guo, Fang Zhou, Hong Wu
Food Bioscience.2024; 61: 104559. CrossRef
Coordinated and Distinct Roles of Peptidoglycan Carboxypeptidases DacC and DacA in Cell Growth and Shape Maintenance under Stress Conditions
Umji Choi, Si Hyoung Park, Han Byeol Lee, Ji Eun Son, Chang-Ro Lee, Cristina Solano
Microbiology Spectrum.2023;[Epub] CrossRef
NoiD, a DedA membrane protein required for homeostasis maintaining of Rhizobium leguminosarum biovar viciae during symbiosis with Pisum sativum
Xiaofang Li, Jiaming Xu, Yajuan Wei, Zirui Chen
Symbiosis.2022; 86(1): 81. CrossRef
Conserved Tandem Arginines for PbgA/YejM Allow Salmonella Typhimurium To Regulate LpxC and Control Lipopolysaccharide Biogenesis during Infection
Nicole P. Giordano, Joshua A. Mettlach, Zachary D. Dalebroux, Manuela Raffatellu
Infection and Immunity.2022;[Epub] CrossRef
Divergent Effects of Peptidoglycan Carboxypeptidase DacA on Intrinsic β-Lactam and Vancomycin Resistance
Si Hyoung Park, Umji Choi, Su-Hyun Ryu, Han Byeol Lee, Jin-Won Lee, Chang-Ro Lee, Krisztina M. Papp-Wallace
Microbiology Spectrum.2022;[Epub] CrossRef
Cryo-EM structure of transmembrane AAA+ protease FtsH in the ADP state
Wu Liu, Martien Schoonen, Tong Wang, Sean McSweeney, Qun Liu
Communications Biology.2022;[Epub] CrossRef
Checkpoints That Regulate Balanced Biosynthesis of Lipopolysaccharide and Its Essentiality in Escherichia coli
Gracjana Klein, Alicja Wieczorek, Martyna Szuster, Satish Raina
International Journal of Molecular Sciences.2021; 23(1): 189. CrossRef

Biophysical characterization of antibacterial compounds derived from pathogenic fungi Ganoderma boninense: Syahriel Abdullah , Yoon Sin Oh , Min-Kyu Kwak , KhimPhin Chong; J. Microbiol. 2021;59(2):164-174. Published online December 23, 2020; DOI: https://doi.org/10.1007/s12275-021-0551-8

56 View
0 Download
8 Web of Science
7 Crossref

Abstract

There have been relatively few studies which support a link between Ganoderma boninense, a phytopathogenic fungus that is particularly cytotoxic and pathogenic to plant tissues and roots, and antimicrobial compounds. We previously observed that liquid-liquid extraction (LLE) using chloroformmethanol- water at a ratio (1:1:1) was superior at detecting antibacterial activities and significant quantities of antibacterial compounds. Herein, we demonstrate that antibacterial secondary metabolites are produced from G. boninense mycelia. Antibacterial compounds were monitored in concurrent biochemical and biophysical experiments. The combined
methods
included high performance thin-layer chromatography (HPTLC), gas chromatography-mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), fourier transform infrared (FTIR), and nuclear magnetic resonance (NMR) spectroscopy. The antibacterial compounds derived from mycelia with chloroform-methanol extraction through LLE were isolated via a gradient solvent elution system using HPTLC. The antibacterial activity of the isolated compounds was observed to be the most potent against Staphylococcus aureus ATCC 25923 and multidrug-resistant S. aureus NCTC 11939. GC-MS, HPLC, and FTIR analysis confirmed two antibacterial compounds, which were identified as 4,4,14α-trimethylcholestane (m/z = 414.75; lanostane, C30H54) and ergosta-5,7,22-trien-3β-ol (m/z = 396.65; ergosterol, C28H44O). With the aid of spectroscopic evaluations, ganoboninketal (m/z = 498.66, C30H42O6), which belongs to the 3,4-seco-27-norlanostane triterpene family, was additionally characterized by 2D-NMR analysis. Despite the lack of antibacterial potential exhibited by lanostane; both ergosterol and ganoboninketal displayed significant antibacterial activities against bacterial pathogens. Results provide evidence for the existence of bioactive compounds in the mycelia of the relatively unexplored phytopathogenic G. boninense, together with a robust method for estimating the corresponding potent antibacterial secondary metabolites.

Citations

Citations to this article as recorded by

Anti-Staphylococcus aureus potential of compounds from Ganoderma sp.: A comprehensive molecular docking and simulation approaches
Trang Thi Thu Nguyen, Trinh Thi Tuyet Nguyen, Hoang Duc Nguyen, Tan Khanh Nguyen, Phu Tran Vinh Pham, Linh Thuy Thi Tran, Hong Khuyen Thi Pham, Phu Chi Hieu Truong, Linh Thuoc Tran, Manh Hung Tran
Heliyon.2024; 10(7): e28118. CrossRef
Medium composition optimization and characterization of polysaccharides extracted from Ganoderma boninense along with antioxidant activity
Qian-Zhu Li, Chuan Xiong, Wei Chee Wong, Li-Wei Zhou
International Journal of Biological Macromolecules.2024; 260: 129528. CrossRef
Plant Defense Inducers and Antioxidant Metabolites Produced During Oil Palm-Ganoderma boninense Interaction In Vitro
Neda Shokrollahi, Chai-Ling Ho, Nur Ain Izzati Mohd Zainudin, Mohd As’wad Bin Abdul Wahab, Mui-Yun Wong
Chemistry Africa.2023; 6(1): 499. CrossRef
Identification of Antibacterial Metabolites from Endophytic Fungus Aspergillus fumigatus, Isolated from Albizia lucidior Leaves (Fabaceae), Utilizing Metabolomic and Molecular Docking Techniques
Mai E. Hussein, Osama G. Mohamed, Ahlam M. El-Fishawy, Hesham I. El-Askary, Amira S. El-Senousy, Ahmed A. El-Beih, Eman S. Nossier, Ahmed M. Naglah, Abdulrahman A. Almehizia, Ashootosh Tripathi, Ahmed A. Hamed
Molecules.2022; 27(3): 1117. CrossRef
Bioactive Compounds of Ganoderma boninense Inhibited Methicillin-Resistant Staphylococcus aureus Growth by Affecting Their Cell Membrane Permeability and Integrity
Yow-San Chan, Khim-Phin Chong
Molecules.2022; 27(3): 838. CrossRef
Review Update on the Life Cycle, Plant–Microbe Interaction, Genomics, Detection and Control Strategies of the Oil Palm Pathogen Ganoderma boninense
Izwan Bharudin, Anis Farhan Fatimi Ab Wahab, Muhammad Asyraff Abd Samad, Ng Xin Yie, Madihah Ahmad Zairun, Farah Diba Abu Bakar, Abdul Munir Abdul Murad
Biology.2022; 11(2): 251. CrossRef
Screening for Antibacterial Activity of French Mushrooms against Pathogenic and Multidrug Resistant Bacteria
Clément Huguet, Mélanie Bourjot, Jean-Michel Bellanger, Gilles Prévost, Aurélie Urbain
Applied Sciences.2022; 12(10): 5229. CrossRef

Review

[MINIREVIEW]Phosphate sugar isomerases and their potential for rare sugar bioconversion: Soo-Jung Kim , Yeong-Su Kim , Soo-Jin Yeom; J. Microbiol. 2020;58(9):725-733. Published online June 25, 2020; DOI: https://doi.org/10.1007/s12275-020-0226-x

53 View
0 Download
8 Web of Science
8 Crossref

Abstract

Phosphate sugar isomerases, catalyzing the isomerization between ketopentose/ketohexose phosphate and aldopentose/ aldohexose phosphate, play an important role in microbial sugar metabolism. They are present in a wide range of microorganisms. They have attracted increasing research interest because of their broad substrate specificity and great potential in the enzymatic production of various rare sugars. Here, the enzymatic properties of various phosphate sugar isomerases are reviewed in terms of their substrate specificities and their applications in the production of valuable rare sugars because of their functions such as low-calorie sweeteners, bulking agents, and pharmaceutical precursor. Specifically, we focused on the industrial applications of D-ribose-5-phosphate isomerase and D-mannose-6-phosphate isomerase to produce D-allose and L-ribose, respectively.

Citations

Citations to this article as recorded by

Production of Value-Added Arabinofuranosyl Nucleotide Analogues from Nucleoside by an In Vitro Enzymatic Synthetic Biosystem
Yuxue Liu, Xiaojing Zhang, Erchu Yang, Xiaobei Liu, Weiwei Su, Zhenyu Wang, Hailei Wang
Biomolecules.2024; 14(11): 1440. CrossRef
Taxonomic Identification of the Arctic Strain Nocardioides Arcticus Sp. Nov. and Global Transcriptomic Analysis in Response to Hydrogen Peroxide Stress
Bailin Cong, Hui Zhang, Shuang Li, Shenghao Liu, Jing Lin, Aifang Deng, Wenqi Liu, Yan Yang
International Journal of Molecular Sciences.2023; 24(18): 13943. CrossRef
A putative glucose 6-phosphate isomerase has pleiotropic functions on virulence and other mechanisms in Acidovorax citrulli
Lynn Heo, Yoobin Han, Yongmin Cho, Junhyeok Choi, Jeongwook Lee, Sang-Wook Han
Frontiers in Plant Science.2023;[Epub] CrossRef
Assessing the genomic composition, putative ecological relevance and biotechnological potential of plasmids from sponge bacterial symbionts
Vanessa Oliveira, Ana R.M. Polónia, Daniel F.R. Cleary, Yusheng M. Huang, Nicole J. de Voogd, Tina Keller-Costa, Rodrigo Costa, Newton C.M. Gomes
Microbiological Research.2022; 265: 127183. CrossRef
Enhanced isomerization of rare sugars by ribose-5-phosphate isomerase A from Ochrobactrum sp. CSL1
Rong Wang, Xinqi Xu, Xuemei Yao, Hengtao Tang, Xin Ju, Liangzhi Li
Enzyme and Microbial Technology.2021; 148: 109789. CrossRef
Simultaneous Production of D-Tagatose, D-Arabitol and Galactitol from Cheese Whey Powder Using Combined Biotransformation and Fermentation Strategies
Guoyan Zhang, Hossain M. Zabed, Yingfeng An, Junhua Yun, Jiaqi Huang, Yufei Zhang, Xiaolan Li, Jiangfei Wang, Xianghui Qi
SSRN Electronic Journal .2021;[Epub] CrossRef
Engineering ribose-5-phosphate isomerase B from a central carbon metabolic enzyme to a promising sugar biocatalyst
Hengtao Tang, Xin Ju, Jing Zhao, Liangzhi Li
Applied Microbiology and Biotechnology.2021; 105(2): 509. CrossRef
Construction of recombinant Escherichia coli expressing xylitol-4-dehydrogenase and optimization for enhanced L-xylulose biotransformation from xylitol
Mesfin Angaw Tesfay, Xin Wen, Yujie Liu, Huibin Lin, Linxu Chen, Jianqiang Lin, Jianqun Lin
Bioprocess and Biosystems Engineering.2021; 44(6): 1021. CrossRef

Journal Articles

Autophagy of bovine mammary epithelial cell induced by intracellular Staphylococcus aureus: Na Geng , Kangping Liu , Jianwei Lu , Yuliang Xu , Xiaozhou Wang , Run Wang , Jianzhu Liu , Yongxia Liu , Bo Han; J. Microbiol. 2020;58(4):320-329. Published online February 26, 2020; DOI: https://doi.org/10.1007/s12275-020-9182-8

47 View
0 Download
19 Web of Science
18 Crossref

Abstract

Bovine mastitis is a common disease in the dairy industry that causes great economic losses. As the primary pathogen of contagious mastitis, Staphylococcus aureus (S. aureus) can invade bovine mammary epithelial cells, thus evading immune defenses and resulting in persistent infection. Recently, autophagy has been considered an important mechanism for host cells to clear intracellular pathogens. In the current study, autophagy caused by S. aureus was detected, and the correlation between autophagy and intracellular S. aureus survival was assessed. First, a model of intracellular S. aureus infection was established. Then, the autophagy of MAC-T cells was evaluated by confocal microscopy and western blot. Moreover, the activation of the PI3K-Akt-mTOR and ERK1/2 signaling pathways was determined by western blot. Finally, the relationship between intracellular bacteria and autophagy was analyzed by using autophagy regulators (3-methyladenine [3-MA], rapamycin [Rapa] and chloroquine [CQ]). The
results
showed that S. aureus caused obvious induction of autophagosome formation, transformation of LC3I/II, and degradation of p62/SQSTM1 in MAC-T cells; furthermore, the PI3K-Akt-mTOR and ERK1/2 signaling pathways were activated. The number of intracellular S. aureus increased significantly with autophagy activation by rapamycin, whereas the number decreased when the autophagy flux was inhibited by chloroquine. Therefore, this study indicated that intracellular S. aureus can induce autophagy and utilize it to survive in bovine mammary epithelial cells.

Citations

Citations to this article as recorded by

Carotenoids as modulators of the PI3K/Akt/mTOR pathway: innovative strategies in cancer therapy
Biswajit Kumar Utpal, Zerrouki Dehbia, B. M. Redwan Matin Zidan, Sherouk Hussein Sweilam, Laliteshwar Pratap Singh, M. S. Arunkumar, M. Sona, Uttam Prasad Panigrahy, R. Keerthana, Sandhya Rani Mandadi, Safia Obaidur Rab, Mohammed Ali Alshehri, Doukani Kou
Medical Oncology.2024;[Epub] CrossRef
Staphylococcus aureus regulates Th17 cells and autophagy via STING in chronic eosinophilic rhinosinusitis with nasal polyps
Weigang Gan, Xingchen Liu, Feng Liu, Junying Hu
European Archives of Oto-Rhino-Laryngology.2024;[Epub] CrossRef
Modulatory Effects of Regulated Cell Death: An Innovative Preventive Approach for the Control of Mastitis
Xiaojing Xia, Pengfei Ren, Yilin Bai, Jingjing Li, Huihui Zhang, Lei Wang, Jianhe Hu, Xinwei Li, Ke Ding
Cells.2024; 13(20): 1699. CrossRef
UID-Dual Transcriptome Sequencing Analysis of the Molecular Interactions between Streptococcus agalactiae ATCC 27956 and Mammary Epithelial Cells
Jishang Gong, Taotao Li, Yuanfei Li, Xinwei Xiong, Jiguo Xu, Xuewen Chai, Youji Ma
Animals.2024; 14(17): 2587. CrossRef
Analysis of differentially expressed microRNAs in bovine mammary epithelial cells treated with lipoteichoic acid
Puxiu Shen, Jingcheng Yu, Chenbo Yan, Dexin Yang, Chao Tong, Xinzhuang Wang
Journal of Animal Physiology and Animal Nutrition.2023; 107(2): 463. CrossRef
PINK1/Parkin‐mediated mitophagy enhances the survival of Staphylococcus aureus in bovine macrophages
Xi Zhou, Kangjun Liu, Jianji Li, Luying Cui, Junsheng Dong, Jun Li, Xia Meng, Guoqiang Zhu, Heng Wang
Journal of Cellular and Molecular Medicine.2023; 27(3): 412. CrossRef
Chlorogenic acid enhances PPARγ-mediated lipogenesis through preventing Lipin 1 nuclear translocation in Staphylococcus aureus-exposed bovine mammary epithelial cells
Ruiyuan Yao, Manshulin Wang, Yue Zhao, Qiang Ji, Xue Feng, Linfeng Bai, Lili Bao, Yanfeng Wang, Huifang Hao, Xihe Li, Zhigang Wang
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids.2023; 1868(11): 159396. CrossRef
Staphylococcus aureus induces mitophagy to promote its survival within bovine mammary epithelial cells
Dianwen Xu, Guiqiu Hu, Jianchun Luo, Ji Cheng, Di Wu, Lisha Cheng, Xuejie Huang, Shoupeng Fu, Juxiong Liu
Veterinary Microbiology.2023; 280: 109697. CrossRef
Subacute ruminal acidosis downregulates FOXA2, changes oxidative status, and induces autophagy in the livers of dairy cows fed a high-concentrate diet
Hongzhu Zhang, Yang Xue, Wan Xie, Yan Wang, Nana Ma, Guangjun Chang, Xiangzhen Shen
Journal of Dairy Science.2023; 106(3): 2007. CrossRef
Activation of PINK1-mediated mitophagy protects bovine mammary epithelial cells against lipopolysaccharide-induced mitochondrial and inflammatory damage in vitro
Renxu Chang, Yan Tang, Hongdou Jia, Zhihao Dong, Shuang Gao, Qian Song, Hao Dong, Qiushi Xu, Qianming Jiang, Juan J. Loor, Xudong Sun, Chuang Xu
Free Radical Biology and Medicine.2023; 194: 172. CrossRef
Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages
Yukang Wen, Zhengkun Chen, Yaqin Tian, Mei Yang, Qingshuang Dong, Yujiao Yang, Honglei Ding
Veterinary Research.2022;[Epub] CrossRef
Academy of Breastfeeding Medicine Clinical Protocol #36: The Mastitis Spectrum, Revised 2022
Katrina B. Mitchell, Helen M. Johnson, Juan Miguel Rodríguez, Anne Eglash, Charlotte Scherzinger, Kyle Widmer, Pamela Berens, Brooke Miller
Breastfeeding Medicine.2022; 17(5): 360. CrossRef
Streptococcus agalactiae-induced autophagy of bovine mammary epithelial cell via PI3K/AKT/mTOR pathway
Mengzhu Qi, Hao Geng, Na Geng, Yukun Cui, Changxi Qi, Guodong Cheng, Kaimin Song, Liping Hu, Yongxia Liu, Jianzhu Liu, Bo Han
Journal of Dairy Research.2022; 89(2): 178. CrossRef
High-dose lipopolysaccharide induced autophagic cell death in bovine mammary alveolar cells
Jin-Ki Park, Joon Mo Yeo, Kwanghyun Cho, Hyun-Jung Park, Won-Young Lee
Journal of Animal Reproduction and Biotechnology.2022; 37(3): 169. CrossRef
Selenium Alleviates Inflammation in Staphylococcus aureus-Induced Mastitis via MerTK-Dependent Activation of the PI3K/Akt/mTOR Pathway in Mice
Si-jie Chen, Chen-yuan Zhang, Di Yu, Chang-jie Lin, Hao-jun Xu, Chang-min Hu
Biological Trace Element Research.2022; 200(4): 1750. CrossRef
Potential role of β‑carotene‑modulated autophagy in puerperal breast inflammation (Review)
Stella Hasianna, Julia Gunadi, Enny Rohmawaty, Ronny Lesmana
Biomedical Reports.2022;[Epub] CrossRef
Intracellular Staphylococcus aureus inhibits autophagy of bovine mammary epithelial cells through activating p38α
Run Wang, Wen Zhang, Lumei Wang, Na Geng, Xiaozhou Wang, Meihua Zhang, Jianzhu Liu, Yongxia Liu, Bo Han
Journal of Dairy Research.2021; 88(3): 293. CrossRef
Autophagy in Staphylococcus aureus Infection
Mengyao Wang, Ziyao Fan, Hongbing Han
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef

Autophagic elimination of Trypanosoma cruzi in the presence of metals: Laís Pessanha de Carvalho , Edésio José Tenório de Melo; J. Microbiol. 2019;57(10):918-926. Published online August 28, 2019; DOI: https://doi.org/10.1007/s12275-019-9018-6

41 View
0 Download
3 Web of Science
3 Crossref

Abstract

Trypanosoma cruzi is an obligate intracellular parasite transmitted to vertebrate hosts by blood-sucking insects. Molecules present in parasites and mammalian cells allow the recognition and parasite internalization. Metallic ions play an essential role in the establishment and maintenance of hostparasite interaction. However, little is known about how parasites handle with essential and nonessential metal quotas. This study aimed to investigate the influence of metal ions on the biological processes of T. cruzi infected cells. Infected cells were incubated with ZnCl2, CdCl2, and HgCl2 for 12 h and labeled with different specific dyes to investigate the cellular events related to intracellular parasite death and elimination. Infected host cells and parasite’s mitochondria underwent functional and structural disorders, in addition to parasite’s DNA condensation and pH decrease on host cells, which led to parasite death. Further investigations suggested that lysosomes were involved in pH decrease and the double membrane of the endoplasmic reticulum formed vacuoles surrounding damaged parasites, which indicate the occurrence of autophagy for parasite elimination. In conclusion, low concentrations of nonessential and essential metals cause a series of damage to Trypanosoma cruzi organelles, leading to its loss of viability, death, and elimination, with no removal of the host cells.

Citations

Citations to this article as recorded by

The role of host autophagy in intracellular protozoan parasites diseases
Rafael Cardoso Maciel Costa Silva, Jhones Sousa Ribeiro, Thalita Santos de Moraes de Farias, Leonardo Holanda Travassos
Archives of Biochemistry and Biophysics.2024; 761: 110186. CrossRef
Personalized Prevention in Mercury-Induced Amyotrophic Lateral Sclerosis: A Case Report
Nicola Magnavita, Mario Sabatelli, Egeria Scoditti, Francesco Chirico
Applied Sciences.2020; 10(21): 7839. CrossRef
Essential and nonessential metal effects on extracellular Leishmania amazonensis in vitro
Laís Pessanha de Carvalho, Jana Held, Edésio José Tenório de Melo
Experimental Parasitology.2020; 209: 107826. CrossRef

Astragaloside IV reversed the autophagy and oxidative stress induced by the intestinal microbiota of AIS in mice: Nan Xu , Pengcheng Kan , Xiuhua Yao , Ping Yang , Jiwei Wang , Lei Xiang , Yu Zhu; J. Microbiol. 2018;56(11):838-846. Published online October 24, 2018; DOI: https://doi.org/10.1007/s12275-018-8327-5

51 View
0 Download
43 Crossref

Abstract

Acute ischaemic stroke (AIS) seriously affects patient quality of life. We explored the role of the intestinal microbiota on oxidative stress and autophagy in stroke, and Astragaloside IV (AS-IV) reversed the changes induced by intestinal microbiota. We determined the characteristics of the intestinal microbiota of AIS and transient ischaemic attack (TIA) patients by 16S sequencing and found that the structure and diversity of the intestinal microbiota in patients with AIS and TIA were significantly different from those in healthy subjects. Specifically, the abundance of genus Bifidobacterium, Megamonas, Blautia, Holdemanella, and Clostridium, content of homocysteine and triglyceride was increased significantly, thus it may be as a potential mechanism of AIS and TIA. Furthermore, germ-free mice were infused intracolonically with fecal supernatants of TIA and AIS with/without feed AS-IV for 12 weeks, and we found that the feces of AIS up-regulated the autophagy markers Beclin-1, light chain 3 (LC3)-II and autophagy-related gene (Atg)12, and the expression of reactive oxygen species (ROS) and NADPH oxidase 2/4 (NOX2/4), malondialdehyde (MDA), however, the expression of total antioxidant capacity (T-AOC) and activity of superoxide dismutase (SOD) and glutathione (GSH) was down-regulated in brain tissue, the content of homocysteine and free fatty acids (FFA) in serum of the mice. Meanwhile, AS-IV could reverse the above phenomenon, however, it does not affect the motor function of mice. AS-IV reversed these changes and it may be a potential drug for AIS therapeutics.

Citations

Citations to this article as recorded by

Astragaloside IV-PESV inhibits prostate cancer tumor growth by restoring gut microbiota and microbial metabolic homeostasis via the AGE-RAGE pathway
Xujun You, Junfeng Qiu, Qixin Li, Qing Zhang, Wen Sheng, Yiguo Cao, Wei Fu
BMC Cancer.2024;[Epub] CrossRef
The impact of dysbiosis in oropharyngeal and gut microbiota on systemic inflammatory response and short-term prognosis in acute ischemic stroke with preceding infection
Qiuxing He, Guoshun Li, Jiasheng Zhao, Huishan Zhu, Huanhao Mo, Zhanshi Xiong, Zhan Zhao, Jingyi Chen, Weimin Ning
Frontiers in Microbiology.2024;[Epub] CrossRef
Role of gut microbiota in ischemic stroke: A narrative review of human and animal studies
Guangtang Chen, Xiaolin Du, Junshuan Cui, Jiaquan Song, Mingsong Xiong, Xi Zeng, Hua Yang, Kaya Xu
Neuroprotection.2024; 2(2): 120. CrossRef
Research progress in the treatment of an immune system disease—type 1 diabetes—by regulating the intestinal flora with Chinese medicine and food homologous drugs
Yang PING, Jianing LIU, Huilin WANG, Yan WANG, Hongbin QIU, Yu ZHANG
Bioscience of Microbiota, Food and Health.2024; 43(3): 150. CrossRef
The relationship between oxidative balance scores and chronic diarrhea and constipation: a population-based study
Jiayan Hu, Hede Zou, Xiyun Qiao, Yuxi Wang, Mi Lv, Kunli Zhang, Fengyun Wang
BMC Public Health.2024;[Epub] CrossRef
Astragaloside IV Mitigated Diabetic Nephropathy by Restructuring Intestinal Microflora and Ferroptosis
Xin Lyu, Ting‐ting Zhang, Zhen Ye, Ce Chen
Molecular Nutrition & Food Research.2024;[Epub] CrossRef
Dietary flavonoids: Role in preventing neurodegenerative diseases caused by brain aging by modulating the gut microbiota
Siyu Liu, Haochen Dai, Rui Wang, Xin Zhang
Food Bioscience.2024; 61: 104965. CrossRef
Notoginsenoside R1 alleviates cerebral ischemia/reperfusion injury by inhibiting the TLR4/MyD88/NF-κB signaling pathway through microbiota-gut-brain axis
Shuxia Zhang, Qiuyan Chen, Meiqi Jin, Jiahui Ren, Xiao Sun, Zhixiu Zhang, Yun Luo, Xiaobo Sun
Phytomedicine.2024; 128: 155530. CrossRef
Astragaloside IV ameliorate acute alcohol-induced liver injury in mice via modulating gut microbiota and regulating NLRP3/caspase-1 signaling pathway
Shan Wu, Fei Wen, Xiangbin Zhong, Wenjing Du, Manlian Chen, Junyi Wang
Annals of Medicine.2023;[Epub] CrossRef
Clinical evidence and mechanisms of traditional Chinese medicine in major diseases
Binyu Luo, Yiwen Li, Wenting Wang, Yanfei Liu, Yanfang Xian, Yue Liu, Keji Chen
Science of Traditional Chinese Medicine.2023;[Epub] CrossRef
New insight into gut microbiota and their metabolites in ischemic stroke: A promising therapeutic target
Shuxia Zhang, Meiqi Jin, Jiahui Ren, Xiao Sun, Zhixiu Zhang, Yun Luo, Xiaobo Sun
Biomedicine & Pharmacotherapy.2023; 162: 114559. CrossRef
Astragaloside IV alleviates neuronal ferroptosis in ischemic stroke by regulating fat mass and obesity‐associated—N6‐methyladenosine—acyl‐CoA synthetase long‐chain family member 4 axis
Zhenglong Jin, Wenying Gao, Fu Guo, Shaojun Liao, Mingzhe Hu, Tao Yu, Shangzhen Yu, Qing Shi
Journal of Neurochemistry.2023; 166(2): 328. CrossRef
Dissecting Causal Relationships Between Gut Microbiota, Blood Metabolites, and Stroke: A Mendelian Randomization Study
Qi Wang, Huajie Dai, Tianzhichao Hou, Yanan Hou, Tiange Wang, Hong Lin, Zhiyun Zhao, Mian Li, Ruizhi Zheng, Shuangyuan Wang, Jieli Lu, Yu Xu, Ruixin Liu, Guang Ning, Weiqing Wang, Yufang Bi, Jie Zheng, Min Xu
Journal of Stroke.2023; 25(3): 350. CrossRef
Progress on traditional Chinese medicine in treatment of ischemic stroke via the gut-brain axis
Zhe Zhai, Pei-Wei Su, Lan-ying Ma, Hui Yang, Tong Wang, Zheng-Gen Fei, Ya-Nan Zhang, Yuan Wang, Ke Ma, Bing-Bing Han, Zhi-Chun Wu, Hua-Yun Yu, Hai-Jun Zhao
Biomedicine & Pharmacotherapy.2023; 157: 114056. CrossRef
Gut microbiota, a hidden protagonist of traditional Chinese medicine for acute ischemic stroke
Lin Gao, Xiuwen Xia, Yinqi Shuai, Hong Zhang, Wei Jin, Xiaoyun Zhang, Yi Zhang
Frontiers in Pharmacology.2023;[Epub] CrossRef
The mechanism of intestinal microbiota regulating immunity and inflammation in ischemic stroke and the role of natural botanical active ingredients in regulating intestinal microbiota: A review
Jinsong Zeng, Kailin Yang, Huifang Nie, Le Yuan, Shanshan Wang, Liuting Zeng, Anqi Ge, Jinwen Ge
Biomedicine & Pharmacotherapy.2023; 157: 114026. CrossRef
Astragaloside IV: A promising natural neuroprotective agent for neurological disorders
Min Yao, Lijuan Zhang, Lin Wang
Biomedicine & Pharmacotherapy.2023; 159: 114229. CrossRef
The effects of astragaloside IV on gut microbiota and serum metabolism in a mice model of intracerebral hemorrhage
Zhilin Li, En Hu, Fei Zheng, Song Wang, Wei Zhang, Jiekun Luo, Tao Tang, Qing Huang, Yang Wang
Phytomedicine.2023; 121: 155086. CrossRef
CONSORT-Characteristics and metabolic phenotype of gut microbiota in NAFLD patients
Haize Ge, Wei Wei, Liang Tang, Yaqiong Tian, Yu Zhu, Yan Luo, Shuye Liu
Medicine.2022; 101(25): e29347. CrossRef
Biological active ingredients of Astragali Radix and its mechanisms in treating cardiovascular and cerebrovascular diseases
Man Li, Bing Han, Huan Zhao, Chongyi Xu, Daokun Xu, Elwira Sieniawska, Xianming Lin, Guoyin Kai
Phytomedicine.2022; 98: 153918. CrossRef
Gut microbes in cerebrovascular diseases: Gut flora imbalance, potential impact mechanisms and promising treatment strategies
Xuelun Zou, Leiyun Wang, Linxiao Xiao, Sai Wang, Le Zhang
Frontiers in Immunology.2022;[Epub] CrossRef
Role of Endogenous Lipopolysaccharides in Neurological Disorders
Manjunath Kalyan, Ahmed Hediyal Tousif, Sharma Sonali, Chandrasekaran Vichitra, Tuladhar Sunanda, Sankar Simla Praveenraj, Bipul Ray, Vasavi Rakesh Gorantla, Wiramon Rungratanawanich, Arehally M. Mahalakshmi, M. Walid Qoronfleh, Tanya M. Monaghan, Byoung-
Cells.2022; 11(24): 4038. CrossRef
Review of the pharmacological effects of astragaloside IV and its autophagic mechanism in association with inflammation
Ying Yang, Meng Hong, Wen-Wen Lian, Zhi Chen
World Journal of Clinical Cases.2022; 10(28): 10004. CrossRef
The Influence of Gut Dysbiosis in the Pathogenesis and Management of Ischemic Stroke
Saravana Babu Chidambaram, Annan Gopinath Rathipriya, Arehally M. Mahalakshmi, Sonali Sharma, Tousif Ahmed Hediyal, Bipul Ray, Tuladhar Sunanda, Wiramon Rungratanawanich, Rajpal Singh Kashyap, M. Walid Qoronfleh, Musthafa Mohamed Essa, Byoung-Joon Song, T
Cells.2022; 11(7): 1239. CrossRef
Astragaloside IV ameliorates cerebral ischemia-reperfusion injury via upregulation of PKA and Cx36
Li Yu, Yuting Wang, Jingxue Tang, Zhaorui Shu, Xian Han
NeuroReport.2022; 33(15): 656. CrossRef
Astragaloside IV ameliorates diet-induced hepatic steatosis in obese mice by inhibiting intestinal FXR via intestinal flora remodeling
Yuanyuan Zhai, Wenling Zhou, Xu Yan, Yuan Qiao, Lingling Guan, Zhichun Zhang, Hao Liu, Jizhi Jiang, Jiang Liu, Liang Peng
Phytomedicine.2022; 107: 154444. CrossRef
Could the Gut Microbiota Serve as a Therapeutic Target in Ischemic Stroke?
Jiyao Zhang, Qiang Tang, Luwen Zhu, San Jun Shi
Evidence-Based Complementary and Alternative Medicine.2021; 2021: 1. CrossRef
Co-exposure to fluoride and arsenic disrupts intestinal flora balance and induces testicular autophagy in offspring rats
Penghui Liu, Ran Li, Xiaolin Tian, Yannan Zhao, Meng Li, Meng Wang, Xiaodong Ying, Jiyu Yuan, Jiaxin Xie, Xiaoting Yan, Yi Lyu, Cailing Wei, Yulan Qiu, Fengjie Tian, Qian Zhao, Xiaoyan Yan
Ecotoxicology and Environmental Safety.2021; 222: 112506. CrossRef
Intestinal Flora: A Pivotal Role in Investigation of Traditional Chinese Medicine
Xiao Li, Dan Wu, Jingjie Niu, Yanping Sun, Qiuhong Wang, Bingyou Yang, Haixue Kuang
The American Journal of Chinese Medicine.2021; 49(02): 237. CrossRef
Role of Polyphenols as Antioxidant Supplementation in Ischemic Stroke
Yuan Zhou, Shanshan Zhang, Xiang Fan, Wen-Jun Tu
Oxidative Medicine and Cellular Longevity.2021;[Epub] CrossRef
Hypoglycemic effect of astragaloside IV via modulating gut microbiota and regulating AMPK/SIRT1 and PI3K/AKT pathway
Pin Gong, Xuyang Xiao, Shuang Wang, Fuxiong Shi, Ni Liu, Xuefeng Chen, Wenjuan Yang, Lan Wang, Fuxin Chen
Journal of Ethnopharmacology.2021; 281: 114558. CrossRef
Reciprocal interactions between gut microbiota and autophagy
Pierre Lapaquette, Jean-Baptiste Bizeau, Niyazi Acar, Marie-Agnès Bringer
World Journal of Gastroenterology.2021; 27(48): 8283. CrossRef
Maternal Obesity Increases Oxidative Stress in Placenta and It Is Associated With Intestinal Microbiota
Chengjun Hu, Yingli Yan, Fengjie Ji, Hanlin Zhou
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Prevention and treatment of chronic heart failure through traditional Chinese medicine: Role of the gut microbiota
Qiujin Jia, Lirong Wang, Xiaonan Zhang, Yuejia Ding, Hao Li, Yingxi Yang, Ao Zhang, Yanyang Li, Shichao Lv, Junping Zhang
Pharmacological Research.2020; 151: 104552. CrossRef
Astragaloside IV as Potential Antioxidant Against Diabetic Ketoacidosis in Juvenile Mice Through Activating JNK/Nrf2 Signaling Pathway
Li-li Deng
Archives of Medical Research.2020; 51(7): 654. CrossRef
Morroniside Inhibits H2O2-Induced Podocyte Apoptosis by Down-Regulating NOX4 Expression Controlled by Autophagy In Vitro
Xue Gao, Yi Liu, Lin Wang, Na Sai, Yixiu Liu, Jian Ni
Frontiers in Pharmacology.2020;[Epub] CrossRef
Astragaloside IV alleviates mouse slow transit constipation by modulating gut microbiota profile and promoting butyric acid generation
Qiulan He, Changpeng Han, Liang Huang, Haojie Yang, Jiancong Hu, Huaxian Chen, Ruoxu Dou, Donglin Ren, Hongcheng Lin
Journal of Cellular and Molecular Medicine.2020; 24(16): 9349. CrossRef
Cross‐Talk between Gut Microbiota and the Heart: A New Target for the Herbal Medicine Treatment of Heart Failure?
Lin Li, Senjie Zhong, Bin Cheng, Hong Qiu, Zhixi Hu, Deborah A. Kennedy
Evidence-Based Complementary and Alternative Medicine.2020;[Epub] CrossRef
Role and significance of traditional Chinese medicine in regulating gastrointestinal microecology to prevent and treat gastrointestinal cancer
Guang-Hui Zhu, Yi-Ting Sang, Jie Li
World Chinese Journal of Digestology.2020; 28(1): 1. CrossRef
Astragaloside IV Protects Against Oxidative Stress in Calf Small Intestine Epithelial Cells via NFE2L2-Antioxidant Response Element Signaling
Yafang Wang, Fugui Jiang, Haijian Cheng, Xiuwen Tan, Yifan Liu, Chen Wei, Enliang Song
International Journal of Molecular Sciences.2019; 20(24): 6131. CrossRef
Astragaloside IV Protects Ethanol-Induced Gastric Mucosal Injury by Preventing Mitochondrial Oxidative Stress and the Activation of Mitochondrial Pathway Apoptosis in Rats
Shumin Qin, Jinjin Yin, Shaogang Huang, Jingyu Lin, Zhigang Fang, Yunsong Zhou, Keer Huang
Frontiers in Pharmacology.2019;[Epub] CrossRef
Astragaloside IV alleviates the symptoms of experimental ulcerative colitis in vitro and in vivo
Suxiao Wu, Zilan Chen
Experimental and Therapeutic Medicine.2019;[Epub] CrossRef
Carboxamide derivatives induce apoptosis in the U251 glioma cell line
Tao Yan, Junxue Zhuang, Lu He
Oncology Letters.2019;[Epub] CrossRef

Review

Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells: Qiang Fu , Huijun Shi , Yan Ren , Fei Guo , Wei Ni , Jun Qiao , Pengyan Wang , Hui Zhang , Chuangfu Chen; J. Microbiol. 2014;52(7):619-625. Published online June 28, 2014; DOI: https://doi.org/10.1007/s12275-014-3479-4

50 View
0 Download
23 Crossref

Abstract

Bovine viral diarrhea virus (BVDV) is an enveloped, positive-sense, single-stranded RNA virus that belongs to the genus Pestivirus (Flaviviridae). The signaling pathways and levels of signaling molecules are altered in Madin-Darby Bovine Kidney (MDBK) cells infected with BVDV. Autophagy is a conservative biological degradation pathway that mainly eliminates and degrades damaged or superfluous organelles and macromolecular complexes for intracellular recycling in eukaryotic cells. Autophagy can also be induced as an effective response to maintain cellular homeostasis in response to different stresses, such as nutrient or growth factor deprivation, hypoxia, reactive oxygen species exposure and pathogen infection. However, the effects of BVDV infection on autophagy inMDBK cells remain unclear. Therefore, we performed an analysis of autophagic activity after BVDV NADL infection using real-time PCR, electron microscopy, laser confocal microscopy, and Western blotting analysis. The results demonstrated that BVDV NADL infection increased autophagic activity and significantly elevated the expression levels of the autophagy-related genes Beclin1 and ATG14 inMDBK cells. However, the knockdown of Beclin1 and ATG14 by RNA interference (RNAi) did not affect BVDV NADL infection-related autophagic activity. These findings provided a novel perspective to elaborate the effects of viral infection on the host cells.

Citations

Citations to this article as recorded by

Non-cytopathic bovine viral diarrhea virus (BVDV) inhibits innate immune responses via induction of mitophagy
Zhijun Li, Ying Zhang, Bao Zhao, Qinghong Xue, Chunjiang Wang, Siyu Wan, Jingyu Wang, Xiwen Chen, Xuefeng Qi
Veterinary Research.2024;[Epub] CrossRef
Immune evasion strategies of bovine viral diarrhea virus
Feng Pang, Qinqin Long, Min Wei
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
bta-miR-2904 inhibits bovine viral diarrhea virus replication by targeting viral-infection-induced autophagy via ATG13
Ningning Yang, Nana Hu, Jiangwei Zhang, Jihai Yi, Zhen Wang, Yong Wang, Peng Wu, Chuangfu Chen
Archives of Virology.2023;[Epub] CrossRef
In Vivo and In Vitro Antiviral Activity of Phlorizin Against Bovine Viral Diarrhea Virus
Zecai Zhang, Jiang Huang, Zhicheng Zhao, Xueying Yuan, Chuang Li, Siyu Liu, Yueqi Cui, Yu Liu, Yulong Zhou, Zhanbo Zhu
Journal of Agricultural and Food Chemistry.2022; 70(47): 14841. CrossRef
The Multi-Faceted Role of Autophagy During Animal Virus Infection
Hui Jiang, Xianjin Kan, Chan Ding, Yingjie Sun
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
DDIT3 Targets Innate Immunity via the DDIT3-OTUD1-MAVS Pathway To Promote Bovine Viral Diarrhea Virus Replication
Song Wang, Peili Hou, Wei Pan, Wenqi He, Daniel Chang He, Hongmei Wang, Hongbin He, Rozanne M. Sandri-Goldin
Journal of Virology.2021;[Epub] CrossRef
Fate of the germ cells in mammalian ovary: A review
Pramod K. Yadav, Anumegha Gupta, Alka Sharma, Anil Kumar Yadav, Meenakshi Tiwari, Ashutosh N. Pandey, Shilpa Prasad, Tulsidas G. Shrivastav, Shail K. Chaube
Journal of Reproductive Health and Medicine.2020; 3: 3. CrossRef
Stress proteins: the biological functions in virus infection, present and challenges for target-based antiviral drug development
Qianya Wan, Dan Song, Huangcan Li, Ming-liang He
Signal Transduction and Targeted Therapy.2020;[Epub] CrossRef
Glucocorticoid-induced autophagy and apoptosis in bone
Tiantian Wang, Xiaonan Liu, Chengqi He
Apoptosis.2020; 25(3-4): 157. CrossRef
Effects of Starvation on Antioxidant-Related Signaling Molecules, Oxidative Stress, and Autophagy in Juvenile Chinese Perch Skeletal Muscle
Ping Wu, Aimin Wang, Jia Cheng, Lin Chen, Yaxiong Pan, Honghui Li, Qi Zhang, Jiaqi Zhang, Wuying Chu, Jianshe Zhang
Marine Biotechnology.2020; 22(1): 81. CrossRef
Autophagy: A Promising Target for Age-related Osteoporosis
Tiantian Wang, Hongchen He, Shaxin Liu, Chengsen Jia, Ziyan Fan, Can Zhong, Jiadan Yu, Honghong Liu, Chengqi He
Current Drug Targets.2019; 20(3): 354. CrossRef
A Comprehensive Review of Autophagy and Its Various Roles in Infectious, Non-Infectious, and Lifestyle Diseases: Current Knowledge and Prospects for Disease Prevention, Novel Drug Design, and Therapy
Rekha Khandia, Maryam Dadar, Ashok Munjal, Kuldeep Dhama, Kumaragurubaran Karthik, Ruchi Tiwari, Mohd. Iqbal Yatoo, Hafiz M.N. Iqbal, Karam Pal Singh, Sunil K. Joshi, Wanpen Chaicumpa
Cells.2019; 8(7): 674. CrossRef
Bovine viral diarrhea virus non-structural protein NS4B induces autophagosomes in bovine kidney cells
Yuto Suda, Shin Murakami, Taisuke Horimoto
Archives of Virology.2019; 164(1): 255. CrossRef
Comprehensive analysis of circRNAs expression profiles in different periods of MDBK cells infected with bovine viral diarrhea virus
Cunyuan Li, Xiaoyue Li, Xiaoxu Hou, Wei Ni, Mengdan Zhang, Huixiang Li, Yueren Xu, Wureli Hazi, Qiman Ma, Yunfeng Zhang, Dawei Wang, Shengwei Hu
Research in Veterinary Science.2019; 125: 52. CrossRef
Germ cell depletion from mammalian ovary: possible involvement of apoptosis and autophagy
Pramod K. Yadav, Meenakshi Tiwari, Anumegha Gupta, Alka Sharma, Shilpa Prasad, Ashutosh N. Pandey, Shail K. Chaube
Journal of Biomedical Science.2018;[Epub] CrossRef
Autophagy as a target for glucocorticoid-induced osteoporosis therapy
Gengyang Shen, Hui Ren, Qi Shang, Ting Qiu, Xiang Yu, Zhida Zhang, Jinjing Huang, Wenhua Zhao, Yuzhuo Zhang, De Liang, Xiaobing Jiang
Cellular and Molecular Life Sciences.2018; 75(15): 2683. CrossRef
Analyses of long non-coding RNAs and mRNA profiling through RNA sequencing of MDBK cells at different stages of bovine viral diarrhea virus infection
Qiman Ma, Liangyuan Li, Yan Tang, Qiang Fu, Sheng Liu, Shengwei Hu, Jun Qiao, Chuangfu Chen, Wei Ni
Research in Veterinary Science.2017; 115: 508. CrossRef
Both cytopathic and non-cytopathic bovine viral diarrhea virus (BVDV) induced autophagy at a similar rate
Mrigendra K.S. Rajput, Karim Abdelsalam, Mahmoud F. Darweesh, Lyle J Braun, Jason Kerkvliet, Adam D. Hoppe, Christopher C.L. Chase
Veterinary Immunology and Immunopathology.2017; 193-194: 1. CrossRef
Roles of Autophagy in Ischemic Heart Diseases and the Modulatory Effects of Chinese Herbal Medicine
Dawei Wang, Weiqing Yu, Yuntao Liu, Guofu Zhong, Zhen Zhao, Xia Yan, Qing Liu
The American Journal of Chinese Medicine.2017; 45(07): 1401. CrossRef
Crosstalk between Autophagy and Apoptosis: Potential and Emerging Therapeutic Targets for Cardiac Diseases
Meng Li, Ping Gao, Junping Zhang
International Journal of Molecular Sciences.2016; 17(3): 332. CrossRef
Roles of p53 and ASF1A in the Reprogramming of Sheep Kidney Cells to Pluripotent Cells
Huijun Shi, Qiang Fu, Guozhong Li, Yan Ren, Shengwei Hu, Wei Ni, Fei Guo, Mengting Shi, Luping Meng, Hui Zhang, Jun Qiao, Zhiru Guo, Chuangfu Chen
Cellular Reprogramming.2015; 17(6): 441. CrossRef
Recent insights into the role of autophagy in the pathogenesis of rheumatoid arthritis
Yujie Dai, Shaoxian Hu
Rheumatology.2015; : kev337. CrossRef
Structures and Functions of Pestivirus Glycoproteins: Not Simply Surface Matters
Fun-In Wang, Ming-Chung Deng, Yu-Liang Huang, Chia-Yi Chang
Viruses.2015; 7(7): 3506. CrossRef

First
Prev
Page of 1
Next
Last

Search