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Research Support, Non-U.S. Gov't
- Surface Display of the HPV L1 Capsid Protein by the Autotransporter Shigella IcsA
- Dan Xu , Xiaofeng Yang , Depu Wang , Jun Yu , Yili Wang
- J. Microbiol. 2014;52(1):77-82. Published online January 4, 2014
- DOI: https://doi.org/10.1007/s12275-014-3235-9
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- 3 Crossref
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Abstract
- Autotransporters have become attractive tools for surface expression of foreign proteins in Gram-negative bacteria. In this study, the Shigella autotransporter IcsA, has been exploited to express the human papillomavirus (HPV) type 16 L1 capsid protein in Shigella sonnei and Escherichia coli. The L1 gene was fused in-frame to replace the coding sequence of the IcsA passenger domain that is responsible for actin-based motility. The resultant hybrid protein could be detected by an anti-L1 antibody on the surface of S. sonnei and E. coli. In E. coli, the protein was expressed on the entire surface of the bacterium. In contrast, the protein was detected mainly at one pole of the Shigella bacterium. However, the protein became evenly distributed on the surface of the Shigella bacterium when the icsP gene was removed. Our study demonstrated the possibility of exploiting autotransporters for surface expression of large, heterologous viral proteins, which may be a useful strategy for vaccine development.
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Citations
Citations to this article as recorded by
- The Trimeric Autotransporter Adhesin SadA from Salmonella spp. as a Novel Bacterial Surface Display System
Shuli Sang, Wenge Song, Lu Lu, Qikun Ou, Yiyan Guan, Haoxia Tao, Yanchun Wang, Chunjie Liu
Vaccines.2024; 12(4): 399. CrossRef - The Shigella ProU system is required for osmotic tolerance and virulence
Rasha Y. Mahmoud, Wenqin Li, Ramadan A. Eldomany, Mohamed Emara, Jun Yu
Virulence.2017; 8(4): 362. CrossRef - The Multivalent Adhesion Molecule SSO1327 plays a key role in Shigella sonnei pathogenesis
Rasha Y. Mahmoud, Daniel Henry Stones, Wenqin Li, Mohamed Emara, Ramadan A. El‐domany, Depu Wang, Yili Wang, Anne Marie Krachler, Jun Yu
Molecular Microbiology.2016; 99(4): 658. CrossRef
- The Trimeric Autotransporter Adhesin SadA from Salmonella spp. as a Novel Bacterial Surface Display System
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