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- An endophytic Coniochaeta velutina producing broad spectrum antimycotics
- Jie Xie , Gary A. Strobel , Tao Feng , Huishuang Ren , Morgan T. Mends , Zeyang Zhou , Brad Geary
- J. Microbiol. 2015;53(6):390-397. Published online May 30, 2015
- DOI: https://doi.org/10.1007/s12275-015-5105-5
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Abstract
- An endophyte (PC27-5) was isolated from stem tissue of Western hemlock (Tsuga heterophylla) in a Pacific Northwest temperate rainforest. Phylogenetic analyses, based on ITS- 5.8S rDNA and 18S rDNA sequence data, combined with cultural and morphological analysis showed that endophyte PC27-5 exhibited all characteristics of a fungus identical to Coniochaeta velutina. Furthermore, wide spectrum antimycotics were produced by this endophyte that were active against such plant pathogens as Sclerotinia sclerotiorum, Pythium ultimum, and Verticillium dahliae and lethal to Phythophthora cinnamomi, Pythium ultimum, and Phytophthora palmivora in plate tests. The bioactive components were purified through organic solvent extraction, followed by silica column chromatography, and finally preparative HPLC. The minimum inhibitory concentration of the active fraction to Pythium ultimum, which was gained from preparative HPLC, was 11 ?/ml. UPLC-HRMS analysis showed there were two similar components in the antimycotic fraction. Their molecular formulae were established as C30H22O11 (compound I) and C30H22O10 (compound II) respectively, and preliminary spectral results indicate that they are anthroquinone glycosides. Other non ?biologically active compounds were identified in culture fluids of this fungus by spectral means as emodin and chrysophanol - anthroquinone derivatives. This is the first report that Coniochaeta velutina as an endophyte produces bioactive antifungal components.
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Citations
Citations to this article as recorded by
- Filamentous Fungi Associated with Disease Symptoms in Non-Native Giant Sequoia (Sequoiadendron giganteum) in Germany—A Gateway for Alien Fungal Pathogens?
Gitta Jutta Langer, Steffen Bien, Johanna Bußkamp
Pathogens.2024; 13(9): 715. CrossRef - Description of Two Fungal Endophytes Isolated from Fragaria chiloensis subsp. chiloensis f. patagonica: Coniochaeta fragariicola sp. nov. and a New Record of Coniochaeta hansenii
Carolina Campos-Quiroz, Jean Franco Castro, Cecilia Santelices, Jorge Carrasco-Fernández, Matías Guerra, Diego Cares-Gatica, Javiera Ortiz-Campos, Yocelyn Ocares, Lorena Barra-Bucarei, Bart Theelen
Taxonomy.2023; 3(2): 183. CrossRef - Analysis of endophytic bacterial flora of mulberry cultivars susceptible and resistant to bacterial wilt using metagenomic sequencing and culture-dependent approach
Ting Yuan, Izhar Hyder Qazi, Peijia Yang, Xueyin Zhang, Jinhao LI, Jiping Liu
World Journal of Microbiology and Biotechnology.2023;[Epub] CrossRef - Endophytes, a Potential Source of Bioactive Compounds to Curtail the Formation–Accumulation of Advanced Glycation End Products: A Review
Lory Sthephany Rochín-Hernández, Lory Jhenifer Rochín-Hernández, Luis Bernardo Flores-Cotera
Molecules.2022; 27(14): 4469. CrossRef - Access to Anti-Biofilm Compounds from Endolichenic Fungi Using a Bioguided Networking Screening
Seinde Toure, Marion Millot, Lucie Ory, Catherine Roullier, Zineb Khaldi, Valentin Pichon, Marion Girardot, Christine Imbert, Lengo Mambu
Journal of Fungi.2022; 8(10): 1012. CrossRef - Seasonal Characterization of the Endophytic Fungal Microbiome of Mulberry (Morus spp.) Cultivars Resistant and Susceptible to Sclerotiniosis
Weifang Xu, Fei Wang, Ruolin Wang, Yuan Sui, Zeyang Zhou, Jie Xie, Zhonghuai Xiang
Microorganisms.2021; 9(10): 2052. CrossRef - We and herbivores eat endophytes
Esperanza Martínez‐Romero, José Luis Aguirre‐Noyola, Rafael Bustamante‐Brito, Pilar González‐Román, Diana Hernández‐Oaxaca, Víctor Higareda‐Alvear, Leslie M. Montes‐Carreto, Julio César Martínez‐Romero, Mónica Rosenblueth, Luis Eduardo Servín‐Garcidueñas
Microbial Biotechnology.2021; 14(4): 1282. CrossRef - Two new endophytic species enrich the Coniochaeta endophytica / C. prunicola clade: Coniochaeta lutea sp. nov. and C. palaoa sp. nov.
A. Elizabeth Arnold, Alison H. Harrington, Jana M. U'Ren, Shuzo Oita, Patrik Inderbitzin
Plant and Fungal Systematics.2021; 66(1): 66. CrossRef - Coniochaeta elegans sp. nov., Coniochaeta montana sp. nov. and Coniochaeta nivea sp. nov., three new species of endophytes with distinctive morphology and functional traits
A. Elizabeth Arnold, Alison H. Harrington, Yu-Ling Huang, Jana M. U'Ren, Nicholas C. Massimo, Victoria Knight-Connoni, Patrik Inderbitzin
International Journal of Systematic and Evolutionary Microbiology.2021;[Epub] CrossRef - Genome expansion by allopolyploidization in the fungal strain Coniochaeta 2T2.1 and its exceptional lignocellulolytic machinery
Stephen J. Mondo, Diego Javier Jiménez, Ronald E. Hector, Anna Lipzen, Mi Yan, Kurt LaButti, Kerrie Barry, Jan Dirk van Elsas, Igor V. Grigoriev, Nancy N. Nichols
Biotechnology for Biofuels.2019;[Epub] CrossRef - Two new cellulolytic fungal species isolated from a 19th-century art collection
Carolina Coronado-Ruiz, Roberto Avendaño, Efraín Escudero-Leyva, Geraldine Conejo-Barboza, Priscila Chaverri, Max Chavarría
Scientific Reports.2018;[Epub] CrossRef - Novel Collophorina and Coniochaeta species from Euphorbia polycaulis, an endemic plant in Iran
Shaghayegh Nasr, Steffen Bien, Mohammad Reza Soudi, Nayyereh Alimadadi, Seyed Abolhassan Shahzadeh Fazeli, Ulrike Damm
Mycological Progress.2018; 17(6): 755. CrossRef
- Filamentous Fungi Associated with Disease Symptoms in Non-Native Giant Sequoia (Sequoiadendron giganteum) in Germany—A Gateway for Alien Fungal Pathogens?
- The Endophyte Curtobacterium flaccumfaciens Reduces Symptoms Caused by Xylella fastidiosa in Catharanthus roseus
- Paulo Teixeira Lacava , Wenbin Li , Welington Luiz Araujo , Joao Lucio Azevedo , John Stephen Hartung
- J. Microbiol. 2007;45(5):388-393.
- DOI: https://doi.org/2599 [pii]
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Abstract
- Citrus variegated chlorosis (CVC) is a disease of the sweet orange [Citrus sinensis (L.)], which is caused by Xylella fastidiosa subsp. pauca, a phytopathogenic bacterium that has been shown to infect all sweet orange cultivars. Sweet orange trees have been occasionally observed to be infected by Xylella fastidiosa without evidencing severe disease symptoms, whereas other trees in the same grove may exhibit severe disease symptoms. The principal endophytic bacterial species isolated from such CVC-asymptomatic citrus plants is Curtobacterium flaccumfaciens. The Madagascar periwinkle [Citrus sinensis (L.)] is a model plant which has been used to study X. fastidiosa in greenhouse environments. In order to characterize the interactions of X. fastidiosa and C. flaccumfaciens, periwinkle plants were inoculated separately with C. flaccumfaciens, X. fastidiosa, and both bacteria together. The number of flowers produced by the plants, the heights of the plants, and the exhibited disease symptoms were evaluated. PCR-primers for C. flaccumfaciens were designed in order to verify the presence of this endophytic bacterium in plant tissue, and to complement an existing assay for X. fastidiosa. These primers were capable of detecting C. flaccumfaciens in the periwinkle in the presence of X. fastidiosa. X. fastidiosa induced stunting and reduced the number of flowers produced by the periwinkle. When C. flaccumfaciens was inoculated together with X. fastidiosa, no stunting was observed. The number of flowers produced by our doubly- inoculated plants was an intermediate between the number produced by the plants inoculated with either of the bacteria separately. Our data indicate that C. flaccumfaciens interacted with X. fastidiosa in C. roseus, and reduced the severity of the disease symptoms induced by X. fastidiosa. Periwinkle is considered to be an excellent experimental system by which the interaction of C. flaccumfaciens and other endophytic bacteria with X. fastidiosa can be studied.
- Morphological, Phylogenetic and Biological Characteristics of Ectropis obliqua Single-Nucleocapsid Nucleopolyhedrovirus
- Xiu-cui Ma , Hai-Jun Xu , Mei-Jun Tang , Qiang Xiao , Jian Hong , Chuan-Xi Zhang
- J. Microbiol. 2006;44(1):77-82.
- DOI: https://doi.org/2333 [pii]
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Abstract
- The tea looper caterpillar, Ectropis obliqua, is one of the major pests of tea bushes. E. obliqua single-nucleocapsid nucleopolyhedrovirus (EcobSNPV) has been used as a commercial pesticide for biocontrol of this insect. However only limited genetic analysis for this important virus has been done up to now. EcobSNPV was characterized in this study. Electron microscopy analysis of the occlusion body showed polyhedra of 0.7 to 1.7 μm in diameter containing a single nucleocapsid per envelope of the virion. A 15.5 kb genomic fragment containing EcoRI-L, EcoRI-N and HindIII-F fragments, was sequenced. Analysis of the sequence revealed that the fragment contained eleven potential open reading frames (ORFs): lef-1, egt, 38.7k, rr1, polyhedrin, orf1629, pk-1, hoar and homologues to Spodoptera exigua multicapsid NPV (SeMNPV) ORFs 15, 28, and 29. Gene arrangement and phylogeny analysis suggest that EcobSNPV is closely related to the previously described Group II NPV. Bioassays on lethal concentration (LC50 and LC90) and lethal time (LT50 and LT90) were conducted to test the susceptibility of E. obliqua larvae to the virus.
- Fungal-sporulation suppressing substances produced by pseudomonas aeruginosa KMCS-1
- Min, Bu Yong , Shim, Jae Young , Kim, Kun Woo , Lee, Jong Kyu , Yoon, Kwon Sang
- J. Microbiol. 1996;34(3):284-288.
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Abstract
- Among the bacteria isolated form compost piles of cattle excretion in a pasture located at the suburbs of Chunchon city, Pseudomonas aeruginosa KMCS-1 was selected for the test of antifungal substances produced. Six fractions were separated by silica gel column chromatography, and then the antifungal activity of each fraction was assayed against Escherichia coli, Bacillus subtilis, Candida albicans, Rhizopus sp., Aspergillus nidulans, Coprinus cinereus, and Pyricularia oryzae by paper disc method. Two fractions showed significant suppressive activities against A. nidulans, C. cinereus, and P. oryzae; however, their mycelial growth was not affected by neither of these fractions. Inhibitory activities of these fractions to sporulation was assayed at the concentration of 50. 25, 12. 5, and 6.25 ㎍/ml and the average inhibition rates against sporulation of A. nidulans, C. cinereus, and P. oryzae were 94.0, 98.3, and 77.9%, respectively. Further purification and analysis of active substances are now being conducted.
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