Journal of Microbiology

Journal Articles

Yeast polyubiquitin unit regulates synaptonemal complex formation and recombination during meiosis: Min-Kyung Jo , Kiwon Rhee , Keun Pil Kim , Soogil Hong; J. Microbiol. 2022;60(7):705-714. Published online July 4, 2022; DOI: https://doi.org/10.1007/s12275-022-2204-y

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Ubiquitin is highly conserved in most eukaryotes and involved in diverse physiological processes, including cell division, protein quality control, and protein degradation mediated by the ubiquitin-proteasome system after heat shock, glucose-starvation, and oxidative stress. However, the role of the ubiquitin gene UBI4, which contains five consecutive head-to-tail ubiquitin repeats, in meiosis has not been investigated. In this study, we show that the Saccharomyces cerevisiae polyubiquitin precursor gene, UBI4, is required to promote synaptonemal complex (SC) formation and suppress excess doublestrand break formation. Moreover, the proportion of Zip1 polycomplexes, which indicate abnormal SC formation, in cells with a mutation in UBI4 (i.e., ubi4Δ cells) is higher than that of wild-type cells, implying that the UBI4 plays an important role in the early meiotic prophase I. Interestingly, although ubi4Δ cells rarely form full-length SCs in the pachytene stage of prophase I, the Zip3 foci are still seen, as in wild-type cells. Moreover, ubi4Δ cells proficiently form crossover and noncrossover products with a slight delay compared to wild-type cells, suggesting that UBI4 is dispensable in SCcoupled recombination. Our findings demonstrate that UBI4 exhibits dual functions that are associated with both positive and negative roles in SC formation and recombination during meiosis.

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The deubiquitinase Usp7 in Drosophila melanogaster is required for synaptonemal complex maintenance
Cathleen M. Lake, Jennifer Gardner, Salam Briggs, Zulin Yu, Grace McKown, R. Scott Hawley
Proceedings of the National Academy of Sciences.2024;[Epub] CrossRef

Autophagy of bovine mammary epithelial cell induced by intracellular Staphylococcus aureus: Na Geng , Kangping Liu , Jianwei Lu , Yuliang Xu , Xiaozhou Wang , Run Wang , Jianzhu Liu , Yongxia Liu , Bo Han; J. Microbiol. 2020;58(4):320-329. Published online February 26, 2020; DOI: https://doi.org/10.1007/s12275-020-9182-8

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Abstract

Bovine mastitis is a common disease in the dairy industry that causes great economic losses. As the primary pathogen of contagious mastitis, Staphylococcus aureus (S. aureus) can invade bovine mammary epithelial cells, thus evading immune defenses and resulting in persistent infection. Recently, autophagy has been considered an important mechanism for host cells to clear intracellular pathogens. In the current study, autophagy caused by S. aureus was detected, and the correlation between autophagy and intracellular S. aureus survival was assessed. First, a model of intracellular S. aureus infection was established. Then, the autophagy of MAC-T cells was evaluated by confocal microscopy and western blot. Moreover, the activation of the PI3K-Akt-mTOR and ERK1/2 signaling pathways was determined by western blot. Finally, the relationship between intracellular bacteria and autophagy was analyzed by using autophagy regulators (3-methyladenine [3-MA], rapamycin [Rapa] and chloroquine [CQ]). The
results
showed that S. aureus caused obvious induction of autophagosome formation, transformation of LC3I/II, and degradation of p62/SQSTM1 in MAC-T cells; furthermore, the PI3K-Akt-mTOR and ERK1/2 signaling pathways were activated. The number of intracellular S. aureus increased significantly with autophagy activation by rapamycin, whereas the number decreased when the autophagy flux was inhibited by chloroquine. Therefore, this study indicated that intracellular S. aureus can induce autophagy and utilize it to survive in bovine mammary epithelial cells.

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Carotenoids as modulators of the PI3K/Akt/mTOR pathway: innovative strategies in cancer therapy
Biswajit Kumar Utpal, Zerrouki Dehbia, B. M. Redwan Matin Zidan, Sherouk Hussein Sweilam, Laliteshwar Pratap Singh, M. S. Arunkumar, M. Sona, Uttam Prasad Panigrahy, R. Keerthana, Sandhya Rani Mandadi, Safia Obaidur Rab, Mohammed Ali Alshehri, Doukani Kou
Medical Oncology.2024;[Epub] CrossRef
Staphylococcus aureus regulates Th17 cells and autophagy via STING in chronic eosinophilic rhinosinusitis with nasal polyps
Weigang Gan, Xingchen Liu, Feng Liu, Junying Hu
European Archives of Oto-Rhino-Laryngology.2024;[Epub] CrossRef
Modulatory Effects of Regulated Cell Death: An Innovative Preventive Approach for the Control of Mastitis
Xiaojing Xia, Pengfei Ren, Yilin Bai, Jingjing Li, Huihui Zhang, Lei Wang, Jianhe Hu, Xinwei Li, Ke Ding
Cells.2024; 13(20): 1699. CrossRef
UID-Dual Transcriptome Sequencing Analysis of the Molecular Interactions between Streptococcus agalactiae ATCC 27956 and Mammary Epithelial Cells
Jishang Gong, Taotao Li, Yuanfei Li, Xinwei Xiong, Jiguo Xu, Xuewen Chai, Youji Ma
Animals.2024; 14(17): 2587. CrossRef
Analysis of differentially expressed microRNAs in bovine mammary epithelial cells treated with lipoteichoic acid
Puxiu Shen, Jingcheng Yu, Chenbo Yan, Dexin Yang, Chao Tong, Xinzhuang Wang
Journal of Animal Physiology and Animal Nutrition.2023; 107(2): 463. CrossRef
PINK1/Parkin‐mediated mitophagy enhances the survival of Staphylococcus aureus in bovine macrophages
Xi Zhou, Kangjun Liu, Jianji Li, Luying Cui, Junsheng Dong, Jun Li, Xia Meng, Guoqiang Zhu, Heng Wang
Journal of Cellular and Molecular Medicine.2023; 27(3): 412. CrossRef
Chlorogenic acid enhances PPARγ-mediated lipogenesis through preventing Lipin 1 nuclear translocation in Staphylococcus aureus-exposed bovine mammary epithelial cells
Ruiyuan Yao, Manshulin Wang, Yue Zhao, Qiang Ji, Xue Feng, Linfeng Bai, Lili Bao, Yanfeng Wang, Huifang Hao, Xihe Li, Zhigang Wang
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids.2023; 1868(11): 159396. CrossRef
Staphylococcus aureus induces mitophagy to promote its survival within bovine mammary epithelial cells
Dianwen Xu, Guiqiu Hu, Jianchun Luo, Ji Cheng, Di Wu, Lisha Cheng, Xuejie Huang, Shoupeng Fu, Juxiong Liu
Veterinary Microbiology.2023; 280: 109697. CrossRef
Subacute ruminal acidosis downregulates FOXA2, changes oxidative status, and induces autophagy in the livers of dairy cows fed a high-concentrate diet
Hongzhu Zhang, Yang Xue, Wan Xie, Yan Wang, Nana Ma, Guangjun Chang, Xiangzhen Shen
Journal of Dairy Science.2023; 106(3): 2007. CrossRef
Activation of PINK1-mediated mitophagy protects bovine mammary epithelial cells against lipopolysaccharide-induced mitochondrial and inflammatory damage in vitro
Renxu Chang, Yan Tang, Hongdou Jia, Zhihao Dong, Shuang Gao, Qian Song, Hao Dong, Qiushi Xu, Qianming Jiang, Juan J. Loor, Xudong Sun, Chuang Xu
Free Radical Biology and Medicine.2023; 194: 172. CrossRef
Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages
Yukang Wen, Zhengkun Chen, Yaqin Tian, Mei Yang, Qingshuang Dong, Yujiao Yang, Honglei Ding
Veterinary Research.2022;[Epub] CrossRef
Academy of Breastfeeding Medicine Clinical Protocol #36: The Mastitis Spectrum, Revised 2022
Katrina B. Mitchell, Helen M. Johnson, Juan Miguel Rodríguez, Anne Eglash, Charlotte Scherzinger, Kyle Widmer, Pamela Berens, Brooke Miller
Breastfeeding Medicine.2022; 17(5): 360. CrossRef
Streptococcus agalactiae-induced autophagy of bovine mammary epithelial cell via PI3K/AKT/mTOR pathway
Mengzhu Qi, Hao Geng, Na Geng, Yukun Cui, Changxi Qi, Guodong Cheng, Kaimin Song, Liping Hu, Yongxia Liu, Jianzhu Liu, Bo Han
Journal of Dairy Research.2022; 89(2): 178. CrossRef
High-dose lipopolysaccharide induced autophagic cell death in bovine mammary alveolar cells
Jin-Ki Park, Joon Mo Yeo, Kwanghyun Cho, Hyun-Jung Park, Won-Young Lee
Journal of Animal Reproduction and Biotechnology.2022; 37(3): 169. CrossRef
Selenium Alleviates Inflammation in Staphylococcus aureus-Induced Mastitis via MerTK-Dependent Activation of the PI3K/Akt/mTOR Pathway in Mice
Si-jie Chen, Chen-yuan Zhang, Di Yu, Chang-jie Lin, Hao-jun Xu, Chang-min Hu
Biological Trace Element Research.2022; 200(4): 1750. CrossRef
Potential role of β‑carotene‑modulated autophagy in puerperal breast inflammation (Review)
Stella Hasianna, Julia Gunadi, Enny Rohmawaty, Ronny Lesmana
Biomedical Reports.2022;[Epub] CrossRef
Intracellular Staphylococcus aureus inhibits autophagy of bovine mammary epithelial cells through activating p38α
Run Wang, Wen Zhang, Lumei Wang, Na Geng, Xiaozhou Wang, Meihua Zhang, Jianzhu Liu, Yongxia Liu, Bo Han
Journal of Dairy Research.2021; 88(3): 293. CrossRef
Autophagy in Staphylococcus aureus Infection
Mengyao Wang, Ziyao Fan, Hongbing Han
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef

Evolution of a major bovine mastitic genotype (rpoB sequence type 10-2) of Staphylococcus aureus in cows: Dae-Sung Ko , Danil Kim , Eun-Kyung Kim , Jae-Hong Kim , Hyuk-Joon Kwon; J. Microbiol. 2019;57(7):587-596. Published online June 27, 2019; DOI: https://doi.org/10.1007/s12275-019-8699-1

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Abstract

Staphylococcus aureus is the major pathogen leading to bovine mastitis globally while livestock-associated methicillin resistant S. aureus (LA-MRSA) has become a potential threat to public health. MRSA from bovine mastitis is not common but a methicillin susceptible S. aureus (MSSA) genotype, rpoB sequence type (RST)10-2 (RST10-2), is prevalent in Korea. To date, many genomic sequences from S. aureus have been elucidated, but the complete genome sequences of RST10-2 MSSA from bovine mastitis has never been reported. In this study, we determined the complete genome sequence of two RST10-2 MSSA that differ from each other in staphylococcal protein A and molecular prophage types [PMB64-1 (t2489/ mPPT0) and PMB81-4 (t127/mPPT1-2-3)] and conducted a comparative genomics study. The genomic sequences of PMB64-1 and PMB81-4 were more homologous to the representative human RST10-2 strains (MSSA476, MW2 etc.) compared to other RSTs. Most of them shared five common pseudogenes, along with high amino acid identity of four variable virulence genes that were identified in this study. However, PMB64-1 and PMB81-4 acquired different strainspecific pseudogenes and mobile genetic elements than the human strains. The unique pseudogene profile and high identity of the virulence genes were verified in RST10-2 field strains from bovine mastitis. Thus, bovine mastitic RST10-2 MSSA may have an evolutionary relationship with the human RST10- 2 community-associated (CA) MSSA and CA-MRSA strains but may have adapted to cows.

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Rapid Antibacterial Activity Assessment of Chimeric Lysins
Jin-Mi Park, Jun-Hyun Kim, Gun Kim, Hun-Ju Sim, Sun-Min Ahn, Kang-Seuk Choi, Hyuk-Joon Kwon
International Journal of Molecular Sciences.2024; 25(4): 2430. CrossRef
Tracing the Evolutionary Pathways of Serogroup O78 Avian Pathogenic Escherichia coli
Eun-Jin Ha, Seung-Min Hong, Seung-Ji Kim, Sun-Min Ahn, Ho-Won Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Antibiotics.2023; 12(12): 1714. CrossRef
Genetic characterization of Staphylococcus aureus isolated from Norway rats in Boston, Massachusetts
Gracen R. Gerbig, Helen Piontkivska, Tara C. Smith, Ruairi White, Jean Mukherjee, Hayley Benson, Marieke Rosenbaum, Jessica H. Leibler
Veterinary Medicine and Science.2023; 9(1): 272. CrossRef
Rapid Screening and Comparison of Chimeric Lysins for Antibacterial Activity against Staphylococcus aureus Strains
Jin-Mi Park, Dae-Sung Ko, Hee-Soo Kim, Nam-Hyung Kim, Eun-Kyoung Kim, Young-Hye Roh, Danil Kim, Jae-Hong Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Antibiotics.2023; 12(4): 667. CrossRef
Comparative genomics of bovine mastitis-origin Staphylococcus aureus strains classified into prevalent human genotypes
Dae-Sung Ko, Nam-Hyung Kim, Eun-Kyung Kim, Eun-Jin Ha, Young-Hye Ro, Danil Kim, Kang-Seuk Choi, Hyuk-Joon Kwon
Research in Veterinary Science.2021; 139: 67. CrossRef

Research Support, Non-U.S. Gov't

Antimicrobial Susceptibilities of Coagulase-Negative Staphylococci (CNS) and Streptococci from Bovine Subclinical Mastitis Cases: Emel Banu Buyukunal Bal , Suleyman Bayar , Mehmet Ali Bal; J. Microbiol. 2010;48(3):267-274. Published online June 23, 2010; DOI: https://doi.org/10.1007/s12275-010-9373-9

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Abstract: The prevalence and antimicrobial susceptibilities of Staphylococci and Streptococci were assessed from subclinical mastitis cases. One hundred Coagulase-Negative Staphylococci (CNS) and 34 Streptoccocci were identified. The most frequently isolated species were Staphylococcus haemolyticus (27%) and Staphylococcus simulans (24%). Susceptible CNS species revealed the highest resistance to penicillin G (58%), ampicillin (48%), neomycin (20%), and oleandomycin (14%). CNS methicillin resistance rates within 82 isolates were 21.95% and 1.22% by disk diffusion and PCR methods, respectively. These results suggested the disk diffusion
method
was more prone to yield false positives. Partial sequencing of the 16S rRNA region from the mecA carrying isolate (S. haemolyticus) was homologous with S. haemolyticus sequences/accessions obtained from GenBank. However, the mecA gene sequence from this isolate was more closely allied with the S. aureus mecA gene of human origins. Identical sequence data was acquired from the National Center for Biotechnology Information (NCBI) database, suggesting horizontal gene transfer between the two species. CNS β-lactamase activity within 81 isolates was 29.63%. The most frequently isolated Streptococcus species were S. uberis (52%) and S. agalactiae (15%). Oleandomycin was the least effective antimicrobial agent on these isolates with 59% susceptibility. Results indicated that CNS and Streptococci exhibited various antimicrobial resistance responses. Consequently, isolation and identification of udder pathogens in herds suffering from subclinical agents is essential to select the most effective antimicrobial agent. Moreover, multiple resistance features of methicillin resistant (MR) isolates should be considered during antimicrobial susceptibility tests.

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