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- SucA-dependent uptake of sucrose across the outer membrane of Caulobacter crescentus
- Samantha K. Modrak , Martha E. Melin , Lisa M. Bowers
- J. Microbiol. 2018;56(9):648-655. Published online July 27, 2018
- DOI: https://doi.org/10.1007/s12275-018-8225-x
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Abstract
- Caulobacter crescentus is an aquatic Gram-negative bacterium that lives in nutrient-poor environments. Like several other aquatic and phytopathogenic bacteria, Caulobacter cells have a relatively large number of genes predicted to encode TonB-dependent receptors (TBDRs). TBDRs transport nutrients across the outer membrane using energy from the proton motive force. We identified one TBDR gene, sucA, which is situated within a cluster of genes predicted to encode a lacIfamily transcription factor (sucR), amylosucrase (sucB), fructokinase (sucC), and an inner membrane transporter (sucD). Given its genomic neighborhood, we proposed that sucA encodes a transporter for sucrose. Using RT-qPCR, we determined that expression of sucABCD is strongly induced by sucrose in the media and repressed by the transcription factor, SucR. Furthermore, cells with a deletion of sucA have a reduced uptake of sucrose. Although cells with a non-polar deletion of sucA can grow with sucrose as the sole carbon source, cells with a polar deletion that eliminates expression of sucABCD cannot grow with sucrose as the sole carbon source. These results show that the suc locus is essential for sucrose utilization while SucA functions as one method of sucrose uptake in Caulobacter crescentus. This work sheds light on a new carbohydrate utilization locus in Caulobacter crescentus.
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- Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida
Rongchao He, Jiajia Wang, Miaozhen Lin, Jing Tian, Bi Wu, Xiaohan Tan, Jianchuan Zhou, Jiachen Zhang, Qingpi Yan, Lixing Huang
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef - Benzo[a]pyrene might be transported by a TonB-dependent transporter in Novosphingobium pentaromativorans US6-1
Jiaqing Liang, Jiantao Xu, Weijun Zhao, Jiaofeng Wang, Kai Chen, Yuqian Li, Yun Tian
Journal of Hazardous Materials.2021; 404: 124037. CrossRef - iTRAQ analysis reveals the effect of gabD and sucA gene knockouts on lysine metabolism and crystal protein formation in Bacillus thuringiensis
Zixian Yi, Tong Zhang, Junyan Xie, Zirong Zhu, Sisi Luo, Kexuan Zhou, Pengji Zhou, Wenhui Chen, Xiaoli Zhao, Yunjun Sun, Liqiu Xia, Xuezhi Ding
Environmental Microbiology.2021; 23(4): 2230. CrossRef - Structure and Stoichiometry of the Ton Molecular Motor
Herve Celia, Nicholas Noinaj, Susan K Buchanan
International Journal of Molecular Sciences.2020; 21(2): 375. CrossRef
- Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida
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