Journal of Microbiology

Journal Articles

CA‑CAS‑01‑A: A Permissive Cell Line for Isolation and Live Attenuated Vaccine Development Against African Swine Fever Virus: Seung-Chul Lee , Yongkwan Kim , Ji-Won Cha , Kiramage Chathuranga , Niranjan Dodantenna , Hyeok-Il Kwon , Min Ho Kim , Weonhwa Jheong , In-Joong Yoon , Joo Young Lee , Sung-Sik Yoo , Jong-Soo Lee; J. Microbiol. 2024;62(2):125-134. Published online March 13, 2024; DOI: https://doi.org/10.1007/s12275-024-00116-1

60 View
0 Download
1 Web of Science
1 Crossref

African swine fever virus (ASFV) is the causative agent of the highly lethal African swine fever disease that affects domestic pigs and wild boars. In spite of the rapid spread of the virus worldwide, there is no licensed vaccine available. The lack of a suitable cell line for ASFV propagation hinders the development of a safe and effective vaccine. For ASFV propagation, primary swine macrophages and monocytes have been widely studied. However, obtaining these cells can be time-consuming and expensive, making them unsuitable for mass vaccine production. The goal of this study was to validate the suitability of novel CA-CAS-01-A (CAS-01) cells, which was identified as a highly permissive cell clone for ASFV replication in the MA-104 parental cell line for live attenuated vaccine development. Through a screening experiment, maximum ASFV replication was observed in the CAS-01 cell compared to other sub-clones of MA-104 with 14.89 and log10 7.5 ± 0.15 Ct value and TCID50/ ml value respectively. When CAS-01 cells are inoculated with ASFV, replication of ASFV was confirmed by Ct value for ASFV DNA, HAD50/ ml assay, TCID50/ ml assay, and cytopathic effects and hemadsoption were observed similar to those in primary porcine alveolar macrophages after 5th passage. Additionally, we demonstrated stable replication and adaptation of ASFV over the serial passage. These results suggest that CAS-01 cells will be a valuable and promising cell line for ASFV isolation, replication, and development of live attenuated vaccines.

Citations

Citations to this article as recorded by

Development and characterization of high-efficiency cell-adapted live attenuated vaccine candidate against African swine fever
Min Ho Kim, Ashan Subasinghe, Yongkwan Kim, Hyeok-Il Kwon, Yehjin Cho, Kiramage Chathuranga, Ji-Won Cha, Ji-Yoon Moon, Ji-Hyeon Hong, Jin Kim, Seung-Chul Lee, Niranjan Dodantenna, Nuwan Gamage, W. A. Gayan Chathuranga, Yeonji Kim, In-Joong Yoon, Joo Young
Emerging Microbes & Infections.2024;[Epub] CrossRef

Tn5 Transposon-based Mutagenesis for Engineering Phage-resistant Strains of Escherichia coli BL21 (DE3): Yinfeng Wang , Guanhua Xuan , Houqi Ning , Jiuna Kong , Hong Lin , Jingxue Wang; J. Microbiol. 2023;61(5):559-569. Published online May 22, 2023; DOI: https://doi.org/10.1007/s12275-023-00048-2

46 View
0 Download

Abstract: Escherichia coli is a preferred strain for recombinant protein production, however, it is often plagued by phage infection during experimental studies and industrial fermentation. While the existing methods of obtaining phage-resistant strains by natural mutation are not efficient enough and time-consuming. Herein, a high-throughput method by combining Tn5 transposon mutation and phage screening was used to produce Escherichia coli BL21 (DE3) phage-resistant strains. Mutant strains PR281-7, PR338-8, PR339-3, PR340-8, and PR347-9 were obtained, and they could effectively resist phage infection. Meanwhile, they had good growth ability, did not contain pseudolysogenic strains, and were controllable. The resultant phage-resistant strains maintained the capabilities of producing recombinant proteins since no difference in mCherry red fluorescent protein expression was found in phage-resistant strains. Comparative genomics showed that PR281-7, PR338-8, PR339-3, and PR340-8 mutated in ecpE, nohD, nrdR, and livM genes, respectively. In this work, a strategy was successfully developed to obtain phage-resistant strains with excellent protein expression characteristics by Tn5 transposon mutation. This study provides a new reference to solve the phage contamination problem.

Activation of the SigE-SigB signaling pathway by inhibition of the respiratory electron transport chain and its effect on rifampicin resistance in Mycobacterium smegmatis: Yuna Oh , Hye-In Lee , Ji-A Jeong , Seonghan Kim , Jeong-Il Oh; J. Microbiol. 2022;60(9):935-947. Published online August 1, 2022; DOI: https://doi.org/10.1007/s12275-022-2202-0

57 View
0 Download
4 Web of Science
4 Crossref

Abstract

Using a mutant of Mycobacterium smegmatis lacking the major aa3 cytochrome c oxidase of the electron transport chain (Δaa3), we demonstrated that inhibition of the respiratory electron transport chain led to an increase in antibiotic resistance of M. smegmatis to isoniazid, rifampicin, ethambutol, and tetracycline. The alternative sigma factors SigB and SigE were shown to be involved in an increase in rifampicin resistance of M. smegmatis induced under respiration-inhibitory conditions. As in Mycobacterium tuberculosis, SigE and SigB form a hierarchical regulatory pathway in M. smegmatis through SigE-dependent transcription of sigB. Expression of sigB and sigE was demonstrated to increase in the Δaa3 mutant, leading to upregulation of the SigB-dependent genes in the mutant. The phoU2 (MSMEG_1605) gene implicated in a phosphatesignaling pathway and the MSMEG_1097 gene encoding a putative glycosyltransferase were identified to be involved in the SigB-dependent enhancement of rifampicin resistance observed for the Δaa3 mutant of M. smegmatis. The significance of this study is that the direct link between the functionality of the respiratory electron transport chain and antibiotic resistance in mycobacteria was demonstrated for the first time using an electron transport chain mutant rather than inhibitors of electron transport chain.

Citations

Citations to this article as recorded by

Rel-dependent decrease in the expression of ribosomal protein genes by inhibition of the respiratory electron transport chain in Mycobacterium smegmatis
Na-Kyeong Kim, Jong-Eun Baek, Ye-Jin Lee, Yuna Oh, Jeong-Il Oh
Frontiers in Microbiology.2024;[Epub] CrossRef
MoaB2, a newly identified transcription factor, binds to σ A in Mycobacterium smegmatis
Barbora Brezovská, Subhash Narasimhan, Michaela Šiková, Hana Šanderová, Tomáš Kovaľ, Nabajyoti Borah, Mahmoud Shoman, Debora Pospíšilová, Viola Vaňková Hausnerová, Dávid Tužinčin, Martin Černý, Jan Komárek, Martina Janoušková, Milada Kambová, Petr Halada,
Journal of Bacteriology.2024;[Epub] CrossRef
Enhanced hypoxanthine utilization for cAMP salvage synthesis efficiently by Arthrobacter sp. CCTCC 2013431 via xanthine oxidase inhibition
Baofeng Chen, Hai Tan, Chang Li, Linbo Li, Zhonghua Zhang, Zhigang Li
Biotechnology Letters.2024; 46(6): 1095. CrossRef
Mycobacterial Regulatory Systems Involved in the Regulation of Gene Expression Under Respiration-Inhibitory Conditions
Yuna Oh, Ha-Na Lee, Eon-Min Ko, Ji-A Jeong, Sae Woong Park, Jeong-Il Oh
Journal of Microbiology.2023; 61(3): 297. CrossRef

Devosia rhizoryzae sp. nov., and Devosia oryziradicis sp. nov., novel plant growth promoting members of the genus Devosia, isolated from the rhizosphere of rice plants: Geeta Chhetri , Inhyup Kim , Minchung Kang , Jiyoun Kim , Yoonseop So , Taegun Seo; J. Microbiol. 2022;60(1):1-10. Published online November 26, 2021; DOI: https://doi.org/10.1007/s12275-022-1474-8

250 View
0 Download
30 Web of Science
32 Crossref

Abstract

Two novel Gram-negative, aerobic, asporogenous, motile, rodshaped, orange and white pigmented, designated as LEGU1T and G19T, were isolated from the roots of rice plants, collected from Goyang, South Korea. Phylogenetic analysis based on their 16S rRNA gene sequences revealed that they belonged to the genus Devosia and formed a different lineage and clusters with different members of the genus Devosia. These strains shared common chemotaxonomic features. In particular, they had Q-10 as the sole quinone, phosphatidylglycerol, diphosphatidylglycerol as the principal polar lipids and C16:0, C18:1 ω7c 11-methyl and summed feature 8 (comprising C18:1 ω7c/ C18:1 ω6c) as the main fatty acids. The draft genome sequences of strains LEGU1T and G19T were 3,524,978 and 3,495,520 bp in size, respectively. Their average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values were 72.8–81.9% and 18.7–25.1%, respectively, with each other and type strains of related species belonging to the genus Devosia, suggesting that these two strains represent novel species. The G + C content of strains LEGU1T and G19T were 62.1 and 63.8%, respectively. Of the two strains, only LEGU1T produced carotenoid and flexirubin-type pigment. Both strains produced siderophore and indole acetic acid (IAA) in the presence of L-tryptophan. Siderophore biosynthesis genes, auxin responsive genes and tryptophan biosynthesis genes were present in their genomes. The present study aimed to determine the detailed taxonomic positions of the strains using the modern polyphasic approach. Based on the results of polyphasic analysis, these strains are suggested to be two novel bacterial species within the genus Devosia. The proposed names are D. rhizoryzae sp. nov., and Devosia oryziradicis sp. nov., respectively. The plant growth promoting effects of these strains suggest that they can be exploited to improve rice crop productivity. The type strain of D. rhizoryzae is LEGU1T (KCTC 82712T = NBRC 114485T) and D. oryziradicis is G19T (KCTC 82688T = NBRC 114842T).

Citations

Citations to this article as recorded by

Chitosan hydrogel microspheres loaded with Bacillus subtilis promote plant growth and reduce chromium uptake
Xia Wang, Zhonglin Yang, Qin Zeng, Xueli Wang, Song Liu, Engui Wang, Yangjin Wu, Yinan Zeng, Maolin He, Yan Wang, Guoqiang Shen, Xuemin Jing, Ren Ping, Xin Zhang, Baodong Chen
International Journal of Biological Macromolecules.2025; 286: 138401. CrossRef
Exploring the Impact of Coconut Peat and Vermiculite on the Rhizosphere Microbiome of Pre-Basic Seed Potatoes under Soilless Cultivation Conditions
Kan Yan, Yanni Ma, Songming Bao, Wandi Li, Yunjiao Wang, Chao Sun, Xin Lu, Juan Ran
Microorganisms.2024; 12(3): 584. CrossRef
Phytoremediation with application of anaerobic fermentation residues regulate the assembly of ecological clusters within co-occurrence network in ionic rare earth tailings soil: A pot experiment
Liumeng Chen, Shasha Chen, Tao Xing, Yun Long, Zhi Wang, Xiaoying Kong, An Xu, Qiangjian Wu, Yongmin Sun
Environmental Pollution.2024; 340: 122790. CrossRef
Devosia lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic Microcystis
Qing-Lin Deng, Yao Xiao, Ru Jia, Xin Xie, Le Wang, Xin Wang, Jian-Yuan Yang, Qi-Gen Guo, Bing-Huo Zhang
Antonie van Leeuwenhoek.2024;[Epub] CrossRef
A tripartite bacterial-fungal-plant symbiosis in the mycorrhiza-shaped microbiome drives plant growth and mycorrhization
Changfeng Zhang, Marcel G. A. van der Heijden, Bethany K. Dodds, Thi Bich Nguyen, Jelle Spooren, Alain Valzano-Held, Marco Cosme, Roeland L. Berendsen
Microbiome.2024;[Epub] CrossRef
Bacillus altitudinis LZP02 improves rice growth by reshaping the rhizosphere microbiome
Weina Chang, Wenjing Chen, Yunlong Hu, Zhigang Wang
Plant and Soil.2024; 498(1-2): 279. CrossRef
Pasture-crop rotations modulate the soil and rhizosphere microbiota and preserve soil structure supporting oat cultivation in the Pampa biome
Victoria Cerecetto, Carolina Leoni, Stephanie D. Jurburg, Ioannis D. Kampouris, Kornelia Smalla, Doreen Babin
Soil Biology and Biochemistry.2024; 195: 109451. CrossRef
Combined effects of azoxystrobin and oxytetracycline on rhizosphere microbiota of Arabidopsis thaliana
Yuke Zhu, Mingjing Ke, Zhitao Yu, Chaotang Lei, Meng Liu, Yaohui Yang, Tao Lu, Ning-Yi Zhou, W.J.G.M. Peijnenburg, Tao Tang, Haifeng Qian
Environment International.2024; 186: 108655. CrossRef
Addition of Chicken Litter Compost Changes Bacteriobiome in Fallow Soil
Natalia Naumova, Pavel Barsukov, Olga Baturina, Olga Rusalimova, Marsel Kabilov
Applied Microbiology.2024; 4(3): 1268. CrossRef
Insights into the interactions of plant-associated bacteria and their role in the transfer of antibiotic resistance genes from soil to plant
Yanping Shen, Baiyu Zhang, Ye Yao, Hanbo Wang, Zhilu Chen, Anjing Hao, Ping Guo
Journal of Hazardous Materials.2024; 480: 135881. CrossRef
Devosia oryzisoli sp. nov., a novel moderately halotolerant bacterium isolated from the roots of rice plants and genome mining revealed the biosynthesis potential as plant growth promoter
Geeta Chhetri, Inhyup Kim, Taegun Seo
Antonie van Leeuwenhoek.2023; 116(3): 231. CrossRef
Distinct rhizobacteria recruitment under copper stress contributes to the different copper-accumulating capacities of two Elsholtzia species (Lamiaceae)
Xiong Li, Gaojuan Zhao, Yingqi Huang, Boqun Li
Plant and Soil.2023; 489(1-2): 295. CrossRef
Effects of above ground pathogen infection and fungicide application on the root-associated microbiota of apple saplings
Maximilian Fernando Becker, A. Michael Klueken, Claudia Knief
Environmental Microbiome.2023;[Epub] CrossRef
Planobacterium oryzisoli sp. nov., a novel bacterium isolated from roots of rice plant
Geeta Chhetri, Inhyup Kim, Sunho Park, Yonghee Jung, Taegun Seo
Archives of Microbiology.2023;[Epub] CrossRef
International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Rhizobia and Agrobacteria. Minutes of the closed annual meeting: videoconference on 11 October 2022 followed by online discussion until 31 December 2022
Seyed Abdollah Mousavi, J. Peter W. Young
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Cooperative Action of Fulvic Acid and Bacillus paralicheniformis Ferment in Regulating Soil Microbiota and Improving Soil Fertility and Plant Resistance to Bacterial Wilt Disease
Xiuyun Zhao, Di Zhu, Jun Tan, Rui Wang, Gaofu Qi, Olubukola Oluranti Babalola
Microbiology Spectrum.2023;[Epub] CrossRef
Paraburkholderia tagetis sp. nov., a novel species isolated from roots of Tagetes patula enhances the growth and yield of Solanum lycopersicum L. (tomato)
Geeta Chhetri, Inhyup Kim, Jiyoun Kim, Yoonseop So, Sunho Park, Yonghee Jung, Taegun Seo
Frontiers in Microbiology.2023;[Epub] CrossRef
Insight into the plant-associated bacterial interactions: Role for plant arsenic extraction and carbon fixation
Yibo Liu, Baiyu Zhang, Ye Yao, Bo Wang, Yiqi Cao, Yanping Shen, Xiaohui Jia, Fukai Xu, Ziwei Song, Chengpeng Zhao, HongJie Gao, Ping Guo
Science of The Total Environment.2023; 894: 164960. CrossRef
Ideonella oryzae sp. nov., isolated from soil, and Spirosoma liriopis sp. nov., isolated from fruits of Liriope platyphylla
Yonghee Jung, Geeta Chhetri, Inhyup Kim, Yoonseop So, Sunho Park, Taegun Seo
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Seasonal Shifts in Bacterial Community Structures in the Lateral Root of Sugar Beet Grown in an Andosol Field in Japan
Seishi Ikeda, Kazuyuki Okazaki, Hiroyuki Takahashi, Hirohito Tsurumaru, Kiwamu Minamisawa
Microbes and Environments.2023; 38(1): n/a. CrossRef
Effects of different cultivation media on root bacterial community characteristics of greenhouse tomatoes
Xinjian Zhang, Qiang Li, Fangyuan Zhou, Susu Fan, Xiaoyan Zhao, Chi Zhang, Kun Yan, Xiaoqing Wu
Frontiers in Microbiology.2023;[Epub] CrossRef
Neoroseomonas alba sp. nov., Neoroseomonas nitratireducens sp. nov., Paraoseomonas indoligenes sp. nov and Paraoseomonas baculiformis sp. nov., isolated from the rhizosphere of paddy soil
Yoonseop So, Geeta Chhetri, Inhyup Kim, Jiyoun Kim, Sunho Park, Yonghee Jung, Taegun Seo
Antonie van Leeuwenhoek.2023; 116(10): 1009. CrossRef
Soil amendment with insect exuviae causes species-specific changes in the rhizosphere bacterial community of cabbage plants
Max Wantulla, Joop J.A. van Loon, Marcel Dicke
Applied Soil Ecology.2023; 188: 104854. CrossRef
Validation List no. 213. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, Markus Göker
International Journal of Systematic and Evolutionary Microbiology .2023;[Epub] CrossRef
Paenibacillus agilis sp. nov., Paenibacillus cremeus sp. nov. and Paenibacillus terricola sp. nov., isolated from rhizosphere soils
Jiyoun Kim, Geeta Chhetri, Inhyup Kim, Yoonseop So, Taegun Seo
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef
An Isolated Arthrobacter sp. Enhances Rice (Oryza sativa L.) Plant Growth
Geeta Chhetri, Inhyup Kim, Minchung Kang, Yoonseop So, Jiyoun Kim, Taegun Seo
Microorganisms.2022; 10(6): 1187. CrossRef
Interactions between Soil Bacterial Diversity and Plant-Parasitic Nematodes in Soybean Plants
Felipe Martins do Rêgo Barros, Alexandre Pedrinho, Lucas William Mendes, Caio César Gomes Freitas, Fernando Dini Andreote, Karyn N. Johnson
Applied and Environmental Microbiology.2022;[Epub] CrossRef
Plant growth promoting bacteria drive food security
Fatimah K. Al-Tammar, A. Y. Z. Khalifa
Brazilian Journal of Biology.2022;[Epub] CrossRef
Chryseobacterium tagetis sp. nov., a plant growth promoting bacterium with an antimicrobial activity isolated from the roots of medicinal plant (Tagetes patula)
Geeta Chhetri, Inhyup Kim, Jiyoun Kim, Yoonseop So, Taegun Seo
The Journal of Antibiotics.2022; 75(6): 312. CrossRef
International Committee on Systematics of Prokaryotes, Subcommittee on the taxonomy of Rhizobia and Agrobacteria, minutes of the annual meeting by videoconference, 5 July 2021, followed by online discussion until 31 December 2021
Seyed Abdollah Mousavi, J. Peter W. Young
International Journal of Systematic and Evolutionary Microbiology.2022;[Epub] CrossRef
Isolation and characterization of Klebsiella oxytoca from the rhizosphere of Lotus corniculatus and its biostimulating features
A. Y. Z. Khalifa, M. F. Aldayel
Brazilian Journal of Biology.2022;[Epub] CrossRef
Shaping effects of rice, wheat, maize, and soybean seedlings on their rhizosphere microbial community
Fan Zhang, Nuohan Xu, Zhenyan Zhang, Qi Zhang, Yaohui Yang, Zhitao Yu, Liwei Sun, Tao Lu, Haifeng Qian
Environmental Science and Pollution Research.2022; 30(13): 35972. CrossRef

Pikeienuella piscinae gen. nov., sp. nov., a novel genus in the family Rhodobacteraceae: Jeeeun Park , Young-Sam Kim , Seong-Jin Kim , Sang-Eon Kim , Hyun-Kyoung Jung , Min-Ju Yu , Young Jae Jeon , Kyoung-Ho Kim; J. Microbiol. 2021;59(6):546-551. Published online April 20, 2021; DOI: https://doi.org/10.1007/s12275-021-0678-7

54 View
0 Download
3 Web of Science
3 Crossref

Abstract

A novel bacterium, designated strain RR4-56T, was isolated from a biofilter of a seawater recirculating aquaculture system. The 16S rRNA gene sequence analysis showed that the isolate was closely related to Halovulum dunhuangense YYQ- 30T (92.6%), Albimonas donghaensis DS2T (91.3%), Pontivivens insulae GYSW-23T (91.3%), and Monaibacterium marinum C7T (90.9%), belonging to the family Rhodobacteraceae. The strain was aerobic, Gram-negative, rod-shaped, oxidasepositive, and catalase-negative. Its optimum temperature, pH, and salinity for growth were 25–30°C, pH 8.5, and 2–3% NaCl (w/v), respectively. Its growth occurred at 15–35°C, pH 5.0–9.5, and 0–7% NaCl (w/v). It contained ubiquinone-10 (Q-10), a respiratory quinone, and the major cellular fatty acids were 11-methyl C18:1 ω7c (31.9%), C18:1 ω6c (30.4%), and C19:0 cyclo ω8c (16.1%). The polar lipids present in the strain were phosphatidylglycerol, an unidentified phospholipid, and an unidentified aminolipid. The strain had one 4,373,045 bp circular chromosome with G + C contents of 65.9 mol% including 4,169 genes, 4,118 coding sequences (CDSs), 3 rRNAs, and 45 tRNAs. Genome annotation predicted some gene clusters related to the degradation of several types of organic matter such as protocatechuate, catechol, and phthalate. Based on the polyphasic characteristics, RR4-56T represents a novel genus and species in the family Rhodobacteraceae, for which the name Pikeienuella piscinae gen. nov., sp. nov. was proposed. The type strain is RR4-56T (= KCTC 52648T = DSM 107918T).

Citations

Citations to this article as recorded by

Systematic degradation process of petroleum hydrocarbons by an integrated bacterial consortium under bottom seawater and surface seawater environments
Lin Lin, Jia Ning, Chunhui Li, Linlin Zhang, Yu Gao, Wang Bo, Ke Shi
Environmental Technology.2024; : 1. CrossRef
Comparing the indigenous microorganism system in typical petroleum-contaminated groundwater
Jili Wang, Yuling Zhang, Yang Ding, Hewei Song, Ting Liu, Yi Zhang, Weiqing Xu, Yujia Shi
Chemosphere.2023; 311: 137173. CrossRef
Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, George Garrity
International Journal of Systematic and Evolutionary Microbiology .2022;[Epub] CrossRef

Research Support, Non-U.S. Gov'ts

Isolation and Characterization of Marine Pigmented Bacteria from Norwegian Coastal Waters and Screening for Carotenoids with UVA-Blue Light Absorbing Properties: Marit H. Stafsnes , Kjell D Josefsen , Geir Kildahl-Andersen , Svein Valla , Trond E. Ellingsen , Per Bruheim; J. Microbiol. 2010;48(1):16-23. Published online March 11, 2010; DOI: https://doi.org/10.1007/s12275-009-0118-6

44 View
0 Download
52 Scopus

Abstract: Microbial culture collections are important resources for isolation of natural compounds with novel properties. In this study, a culture collection of around 1,500 pigmented heterotrophic bacteria was established. The bacteria were isolated from the sea surface microlayer at different sampling sites along the mid-part of the Norwegian coast. The bacterial isolates produced pigments of various coloration (e.g. golden, yellow, red, pink and orange). Methanol extracts of sixteen isolates were characterized with LC-Diodearray-TOF mass spectrometry analysis. The number of pigments per isolate varied considerably, and a tentative identification of the pigments was performed based on UV-absorbance profile and molecular formula assignation based on the accurate mass determination. The LC-MS analyses evealed that most of the pigments probably were carotenoids. Furthermore, we developed a high throughput LC-MS method for characterization and screening of a larger sub-fraction (300 isolates) of the culture collection. The aim was to screen and identify bacterial isolates producing carotenoids that absorb light in the UVA-Blue light. Six of the bacterial strains were selected for detailed investigation, including 16s rRNA sequencing, preparative HPLC for purification of major carotenoids and subsequent structural elucidation with NMR. Among the identified carotenoids were zeaxanthin, nostoxanthin and sarcinaxanthin, some with novel glycosylation patterns.

Increased Carotenoid Production in Xanthophyllomyces dendrorhous G276 Using Plant Extracts: Soo-Ki Kim , Jun-Hyeong Lee , Chi-Ho Lee , Yoh-Chang Yoon; J. Microbiol. 2007;45(2):128-132.; DOI: https://doi.org/2523 [pii]

40 View
0 Download

Abstract: The red yeast Xanthophyllomyces dendrorhous (previously named Phaffia rhodozyma) produces astaxanthin pigment among many carotenoids. The mutant X. dendrorhous G276 was isolated by chemical mutagenesis. The mutant produced about 2.0 mg of carotenoid per g of yeast cell dry weight and 8.0 mg/L of carotenoid after 5 days batch culture with YM media; in comparison, the parent strain produced 0.66 mg/g of yeast cell dry weight and a carotenoid concentration of 4.5 mg/L. We characterized the utilization of carbon sources by the mutant strain and screened various edible plant extracts to enhance the carotenoid production. The addition of Perilla frutescens (final concentration, 5%) or Allium fistulosum extracts (final concentration, 1%) enhanced the pigment production to about 32 mg/L. In a batch fermentor, addition of Perilla frutescens extract reduced the cultivation time by two days compared to control (no extract), which usually required five-day incubation to fully produce astaxanthin. The results suggest that plant extracts such as Perilla frutescens can effectively enhance astaxanthin production.

Expression and Activity of Citrus Phytoene Synthase and [beta]-Carotene Hydroxylase in Escherichia coli: In-Jung Kim , Kyong-Cheol Ko , Tae-Sik Nam , Yu-Wang Kim , Won-Il Chung , Chan-Shick Kim; J. Microbiol. 2003;41(3):212-218.

36 View
0 Download

Abstract: Citrus phytoene synthase (CitPsy) and [beta]-carotene hydroxylase (CitChx), which are involved in caroteinoid biosynthesis, are distantly related to the corresponding bacterial enzymes from the point of view of amino acid sequence similarity. We investigated these enzyme activities using Pantoea ananatis carotenoid biosynthetic genes and Escherichia coli as a host cell. The genes were cloned into two vector systems controlled by the T7 promoter. SDS-polyacrylamide gel electrophoresis showed that CitPsy and CitChx proteins are normally expressed in E. coli in both soluble and insoluble forms. In vivo complementation using the Pantoea ananatis enzymes and HPLC analysis showed that [beta]-carotene and zeaxanthin were produced in recombinant E. coli, which indicated that the citrus enzymes were functionally expressed in E. coli and assembled into a functional multi-enzyme complex with Pantoea ananatis enzymes. These observed activities well matched the results of other researchers on tomato phytoene synthase and Arabidopsis and pepper [beta]-carotene hydroxylases. Thus, our results suggest that plant carotenoid biosynthetic enzymes can generally complement the bacterial enzymes and could be a means of carotenoid production by molecular breeding and fermentation in bacterial and plant systems.

First
Prev
Page of 1
Next
Last

Search