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- Cotesia plutellae Bracovirus Suppresses Expression of an Antimicrobial Peptide, Cecropin, in the Diamondback Moth, Plutella xylostella, Challenged by Bacteria
- Karen P. Barandoc , Jaehyun Kim , Yonggyun Kim
- J. Microbiol. 2010;48(1):117-123. Published online March 11, 2010
- DOI: https://doi.org/10.1007/s12275-009-9261-3
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Abstract
- An endoparasitoid wasp, Cotesia plutellae, induces significant immunosuppression of host insect, Plutella xylostella. This study was focused on suppression in humoral immune response of P. xylostella parasitized by C. plutellae. An EST database of P. xylostella provided a putative cecropin gene (PxCec) which is 627 bp long and encodes 66 amino acids. A signal peptide (22 amino acids) is predicted and two putative O-glycosylation sites in threonine are located at positions 58 and 64. Without bacterial infection, PxCec was expressed in pupa and adult stages but not in the egg and larval stages. Upon bacterial challenge, however, the larvae expressed PxCec as early as 3 h post infection (PI) and maintained high expression levels at 12-24 h PI. By 48 h PI, its expression noticeably diminished. All tested tissues of bacteria-infected P. xylostella showed PxCec expression. However, other microbes, such as virus and fungus, did not induce the PxCec expression. Parasitization by C. plutellae suppressed the expression of PxCec in response to bacterial challenge. Among the parasitic factors of C. plutellae, its symbiotic virus (C. plutellae bracovirus: CpBV) alone was able to inhibit the expression of PxCec of P. xylostella challenged by bacteria. These results indicate that PxCec expression is regulated by both immune and developmental processes in P. xylostella. The parasitization by C. plutellae inhibited the expression of PxCec by the wasp’s symbiotic virus.
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Citations
Citations to this article as recorded by
- Identification and characteristics of a novel cecropin from the armyworm, Mythimna separata
Kaiqi Lian, Mingliang Zhang, Xiuli Liang, Lingling Zhou, Zhiqi Shi, Yajie Tang, Xueping Wang, Yuwei Song, Yuanchen Zhang
BMC Microbiology.2020;[Epub] CrossRef - Induction of stress‐ and immune‐associated genes in the Indian meal moth Plodia interpunctella against envenomation by the ectoparasitoid Bracon hebetor
Tahir Shafeeq, Zain UlAbdin, Kyeong‐Yeoll Lee
Archives of Insect Biochemistry and Physiology.2017;[Epub] CrossRef - Venom of Parasitoid Pteromalus puparum Impairs Host Humoral Antimicrobial Activity by Decreasing Host Cecropin and Lysozyme Gene Expression
Qi Fang, Bei-Bei Wang, Xin-Hai Ye, Fei Wang, Gong-Yin Ye
Toxins.2016; 8(2): 52. CrossRef - Antiviral activity of the inducible humoral immunity and its suppression by eleven BEN family members encoded in Cotesia plutellae bracovirus
Md. Ramjan Ali, Yonggyun Kim
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology.2015; 179: 44. CrossRef - ANTI‐INFLAMMATORY ACTIVITIES OF CECROPIN A AND ITS MECHANISM OF ACTION
Eunjung Lee, Areum Shin, Yangmee Kim
Archives of Insect Biochemistry and Physiology.2015; 88(1): 31. CrossRef - Transcriptome Immune Analysis of the Invasive Beetle Octodonta nipae (Maulik) (Coleoptera: Chrysomelidae) Parasitized by Tetrastichus brontispae Ferrière (Hymenoptera: Eulophidae)
Baozhen Tang, Jun Chen, Youming Hou, E. Meng, Irene Söderhäll
PLoS ONE.2014; 9(3): e91482. CrossRef - Parasitoid polydnaviruses: evolution, pathology and applications
Dawn Gundersen-Rindal, Catherine Dupuy, Elisabeth Huguet, Jean-Michel Drezen
Biocontrol Science and Technology.2013; 23(1): 1. CrossRef - Parasitization by Cotesia chilonis Influences Gene Expression in Fatbody and Hemocytes of Chilo suppressalis
Shun-Fan Wu, Fang-Da Sun, Yi-Xiang Qi, Yao Yao, Qi Fang, Jia Huang, David Stanley, Gong-Yin Ye, Ben J. Mans
PLoS ONE.2013; 8(9): e74309. CrossRef - Production, purification, and characterization of the cecropin from Plutella xylostella, pxCECA1, using an intein-induced self-cleavable system in Escherichia coli
Hong Wang, Xiao-lin Meng, Jin-ping Xu, Jian Wang, Hua Wang, Chun-wei Ma
Applied Microbiology and Biotechnology.2012; 94(4): 1031. CrossRef - Cecropins as a marker of Spodoptera frugiperda immunosuppression during entomopathogenic bacterial challenge
B. Duvic, V. Jouan, N. Essa, P.-A. Girard, S. Pagès, Z. Abi Khattar, N.-A. Volkoff, A. Givaudan, D. Destoumieux-Garzon, J.-M. Escoubas
Journal of Insect Physiology.2012; 58(6): 881. CrossRef - Deep sequencing-based transcriptome analysis of Plutella xylostella larvae parasitized by Diadegma semiclausum
Kayvan Etebari, Robin W Palfreyman, David Schlipalius, Lars K Nielsen, Richard V Glatz, Sassan Asgari
BMC Genomics.2011;[Epub] CrossRef
- Identification and characteristics of a novel cecropin from the armyworm, Mythimna separata
- Structure-antifungal activity relationships of cecropin a hybrid peptides against trichoderma sp.
- Shin, Song Yub , Lee, Dong Gun , Lee, Sung gu , Kim, Kil Lyong , Lee, Myung Kyu , Hahm, Kyung Soo
- J. Microbiol. 1997;35(1):21-24.
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Abstract
- The hybrid peptides, CA-ME, CA-MA and CA-BO, with the N-terminal sequence 1-8 of cecropin A and the N-terminal sequences 1-12 of melittin, magainin 2 and bombinin, respectively, have more improved antibacterial activities. CA-MA was found to have stronger antifungal activity against Trichoderma sp than other hybrid peptides and their parental peptides. In order to elucidate the relationships between the peptide structure and antifungal activity, several analogues of CA-MA or CA-BO were also designed and synthesized by the solid phase method. Antifungal activity was measured against T. reesei and T. viride, and hemolytic activity was measured by a solution method against human red blood cells. The residue 16 of CA-MA, Ser, was found to be important for antifungal activity. When the residue was substituted with Leu, showed powerful antifungal activity was dramatically decreased. CA-MA, P1, P4 and P5 designed in this study showed powerful antifungal activity against T. reesei and T. viride with low hemolytic activity against human red blood cells. These hybrid peptides will be potentially useful model to further design peptides with powerful antifungal activity for the effective therepy of fungal infection and understand the mechanisms of antifungal actions of hybrid peptides.
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