Research Support, Non-U.S. Gov'ts
- The Identification of Six Novel Proteins with Fibronectin or Collagen Type І Binding Activity from Streptococcus suis Serotype 2
-
Hui Zhang , Junxi Zheng , Li Yi , Yue Li , Zhe Ma , Hongjie Fan , Chengping Lu
-
J. Microbiol. 2014;52(11):963-969. Published online October 31, 2014
-
DOI: https://doi.org/10.1007/s12275-014-4311-x
-
-
48
View
-
0
Download
-
12
Crossref
-
Abstract
-
Streptococcus suis, a major swine pathogen, is an emerging zoonotic agent that causes meningitis and septic shock. Bacterial cell wall and secreted proteins are often involved in interactions with extracellular matrix proteins (ECMs), which play important roles in the initial steps of pathogenesis. In this study, 2D SDS-PAGE, western blotting-based binding affinity measurements, and microtiter plate binding assays were used to identify cell wall and secreted proteins from S. suis that interact with fibronectin and collagen type І. We identified six proteins from S. suis, including three proteins (translation elongation factor G, oligopeptide-binding protein
OppA precursor, and phosphoglycerate mutase) that show both fibronectin and collagen type І binding activity. To the best of our knowledge, these three newly identified proteins had no previously reported fibronectin or collagen type І binding activity. Overall, the aim in this study was to identify proteins with ECM binding activity from S. suis and it represents the first report of six new proteins from S. suis
that interact with fibronectin or collagen type І.
-
Citations
Citations to this article as recorded by

- Orphan response regulator CovR plays positive regulative functions in the survivability and pathogenicity of Streptococcus suis serotype 2 isolated from a pig
Yanyan Zhang, Rui Li, Qian Li, Yongwei Zhu, Xiaopei Yang, Di Zhao, Bingbing Zong
BMC Veterinary Research.2023;[Epub] CrossRef - Schistosoma mansoni phosphoglycerate mutase: a glycolytic ectoenzyme with thrombolytic potential
David B. Pirovich, Akram A. Da’dara, Patrick J. Skelly
Parasite.2022; 29: 41. CrossRef - Quantitative proteomic analysis reveals that serine/threonine kinase is involved in Streptococcus suis virulence and adaption to stress conditions
Haodan Zhu, Junming Zhou, Dandan Wang, Zhengyu Yu, Bin Li, Yanxiu Ni, Kongwang He
Archives of Microbiology.2021; 203(7): 4715. CrossRef - Identification of novel pig and human immunoglobulin G-binding proteins and characterization of the binding regions of enolase from Streptococcus suis serotype 2
Quan Li, Yang Fu, Genglin Guo, Zhuohao Wang, Wei Zhang
AMB Express.2020;[Epub] CrossRef - Identification of two adhesins of Actinobacillus seminis
J. Fernando Montes-García, Delil A. Chincoya Martinez, Sergio Vaca Pacheco, Candelario Vázquez Cruz, Patricia Sanchez Alonso, Juan Xicohtencatl Cortes, Hector Trujillo-Ruiz, Erasmo Negrete-Abascal
Small Ruminant Research.2018; 167: 100. CrossRef - Characterization and functional analysis of PnuC that is involved in the oxidative stress tolerance and virulence of Streptococcus suis serotype 2
Quan Li, Yuhang Zhang, Du Dechao, Yu Yanfei, Wei Zhang
Veterinary Microbiology.2018; 216: 198. CrossRef - Understanding the virulence of Streptococcus suis : A veterinary, medical, and economic challenge
B. Haas, D. Grenier
Médecine et Maladies Infectieuses.2018; 48(3): 159. CrossRef - Factor H specifically capture novel Factor H-binding proteins of Streptococcus suis and contribute to the virulence of the bacteria
Quan Li, Caifeng Ma, Yang Fu, Yanan He, Yanfei Yu, Dechao Du, Huochun Yao, Chengping Lu, Wei Zhang
Microbiological Research.2017; 196: 17. CrossRef - The surface-displayed chaperones GroEL and DnaK of Mycoplasma pneumoniae interact with human plasminogen and components of the extracellular matrix
Lisa Hagemann, Anne Gründel, Enno Jacobs, Roger Dumke
Pathogens and Disease.2017;[Epub] CrossRef - Initial steps of the pathogenesis of the infection caused byStreptococcus suis: fighting against nonspecific defenses
Mariela Segura, Cynthia Calzas, Daniel Grenier, Marcelo Gottschalk
FEBS Letters.2016; 590(21): 3772. CrossRef - Interactions of surface-displayed glycolytic enzymes of Mycoplasma pneumoniae with components of the human extracellular matrix
Anne Gründel, Enno Jacobs, Roger Dumke
International Journal of Medical Microbiology.2016; 306(8): 675. CrossRef - Identification of Novel Laminin- and Fibronectin-binding Proteins by Far-Western Blot: Capturing the Adhesins of Streptococcus suis Type 2
Quan Li, Hanze Liu, Dechao Du, Yanfei Yu, Caifeng Ma, Fangfang Jiao, Huochun Yao, Chengping Lu, Wei Zhang
Frontiers in Cellular and Infection Microbiology.2015;[Epub] CrossRef
- Enhanced Secretion of Cell Wall Bound Enolase into Culture Medium by the soo1-1 Mutation of Saccharomyces cerevisiae
-
Ki-Hyun Kim , Hee-Moon Park
-
J. Microbiol. 2004;42(3):248-252.
-
DOI: https://doi.org/2080 [pii]
-
-
Abstract
-
In order to identify the protein(s) secreted into culture medium by the soo1-1/ret1-1 mutation of Saccharomyces cerevisiae, proteins from the culture medium of cells grown at permissive (28^oC) and non-permissive temperatures (37^oC), were analyzed. Comparison of protein bands separated by SDS-PAGE identified a prominent band of 47-kDa band from a mutant grown at 37^oC. N-terminal amino acid sequencing of this 47-kDa protein showed high identity with enolases 1 and 2. Western blot analysis revealed that most of the cell wall-bound enolase was released into the culture medium of the mutant grown at 37^oC, some of which were separated as those with lower molecular weights. Our results, presented here, indicate the impairment of cell wall enolase biogenesis and assembly by the soo1-1/ret1-1 mutation of S. cerevisiae.