Journal of Microbiology

Journal Article

Heterologous expression and enzymatic characterization of γ-glutamyltranspeptidase from Bacillus amyloliquefaciens: Jung-Min Lee , Jaejung Lee , Gyeong-Hwa Nam , Byung-Sam Son , Myoung-Uoon Jang , So-Won Lee , Byung-Serk Hurh , Tae-Jip Kim; J. Microbiol. 2017;55(2):147-152. Published online January 26, 2017; DOI: https://doi.org/10.1007/s12275-017-6638-6

66 View
0 Download
15 Crossref

γ-Glutamyltranspeptidase (GGT) catalyzes the cleavage of γ- glutamyl compounds and the transfer of γ-glutamyl moiety to water or to amino acid/peptide acceptors. GGT can be utilized for the generation of γ-glutamyl peptides or glutamic acid, which are used as food taste enhancers. In the present study, Bacillus amyloliquefaciens SMB469 with high GGT activity was isolated from Doenjang, a traditional fermented soy food of Korea. The gene encoding GGT from B. amyloliquefaciens SMB469 (BaGGT469) was cloned from the isolate, and heterologously expressed in E. coli and B. subtilis. For comparison, three additional GGT genes were cloned from B. subtilis 168, B. licheniformis DSM 13, and B. amyloliquefaciens FZB42. The BaGGT469 protein was composed of 591 amino acids. The final protein comprises two separate polypeptide chains of 45.7 and 19.7 kDa, generated via autocatalytic cleavage. The specific activity of BaGGT469 was determined to be 17.8 U/mg with γ-L-glutamyl-p-nitroanilide as the substrate and diglycine as the acceptor. GGTs from B. amyloliquefaciens showed 1.4- and 1.7-fold higher transpeptidase activities than those from B. subtilis and B. licheniformis, respectively. Especially, recombinant B. subtilis expressing BaGGT469 demonstrated 11- and 23-fold higher GGT activity than recombinant E. coli and the native B. amyloliquefaciens, respectively, did. These results suggest that BaGGT469 can be utilized for the enzymatic production of various γ- glutamyl compounds.

Citations

Citations to this article as recorded by

Exploring the capacity of Bacillus species for production of kokumi γ-glutamyl peptides: A comparative study
Qian Li, Longteng Zhang, Dennis Sandris Nielsen, Lene Jespersen, René Lametsch
LWT.2024; 206: 116542. CrossRef
Functional connexion of bacterioferritin in antibiotic production and morphological differentiation in Streptomyces coelicolor
Javier García-Martín, Laura García-Abad, Ramón I. Santamaría, Margarita Díaz
Microbial Cell Factories.2024;[Epub] CrossRef
Efficient expression of γ-glutamyl transpeptidase in Bacillus subtilis via CRISPR/Cas9n and its immobilization
Qianlin Chen, Bin Wang, Li Pan
Applied Microbiology and Biotechnology.2024;[Epub] CrossRef
Biochemical Characterization of γ-Glutamyl Transpeptidase from Bacillus altitudinis IHB B1644 and Its Application in the Synthesis of l-Theanine
Eshita Sharma, Milan Kumar Lal, Arvind Gulati, Ashu Gulati
Journal of Agricultural and Food Chemistry.2023; 71(14): 5592. CrossRef
Characterization of alkaline Bacillus amyloliquefaciens γ-glutamyltranspeptidase expressed in Bacillus subtilis and its application in enzymatic synthesis of L‑Theanine
Ran Zhang, Luhua Zheng, Licheng Zhou, Longbei Xiang, Bo Jiang, Tao Zhang, Jingjing Chen
Process Biochemistry.2023; 131: 125. CrossRef
Heterologous expression, on-column refolding and characterization of gamma-glutamyl transpeptidase gene from Bacillus altitudinis IHB B1644: A microbial bioresource from Western Himalayas
Eshita Sharma, Milan Kumar Lal, Arvind Gulati, Ashu Gulati
Process Biochemistry.2022; 116: 126. CrossRef
Expression of Bacillus amyloliquefaciens γ-Glutamyltransferase in Lactococcus lactis and Immobilization on Magnetic Nanoparticles
Tiange Ma, Xingjiang Li, Xuefeng Wu, Suwei Jiang, Zhi Zheng, Dongdong Mu
ACS Food Science & Technology.2021; 1(5): 778. CrossRef
Bacterial Gamma-Glutamyl Transpeptidase, an Emerging Biocatalyst: Insights Into Structure–Function Relationship and Its Biotechnological Applications
Meenu Saini, Amuliya Kashyap, Shruti Bindal, Kuldeep Saini, Rani Gupta
Frontiers in Microbiology.2021;[Epub] CrossRef
A chemical screening method for menaquinone-producing strains based on HPLC-UV technology
Sheng Cao, Xia Du, Pingyi Li, Ganjun Yuan, Shanjun Chen, Weiping Chen, Xiaoyuan Song, Bingdi Kuang
Journal of Microbiological Methods.2020; 172: 105907. CrossRef
γ-Glutamyltranspeptidase from Bacillus amyloliquefaciens: transpeptidation activity enhancement and L-theanine production
Zelong Li, Runtao Zhu, Yongqi Liu, Jiaqi Li, Haofeng Gao, Nan Hu
Enzyme and Microbial Technology.2020; 140: 109644. CrossRef
Secretion of Bacillus amyloliquefaciens γ-Glutamyltranspeptidase from Bacillus subtilis and Its Application in Enzymatic Synthesis of l-Theanine
Dongdong Mu, Haowen Li, Qi Chen, Jing Zhu, Xuefeng Wu, Shuizhong Luo, Yanyan Zhao, Lei Wang, Shaotong Jiang, Xingjiang Li, Zhi Zheng
Journal of Agricultural and Food Chemistry.2019; 67(51): 14129. CrossRef
High level extracellular production of recombinant γ-glutamyl transpeptidase from Bacillus licheniformis in Escherichia coli fed-batch culture
Shruti Bindal, Vikas Kumar Dagar, Meenu Saini, Yogender Pal Khasa, Rani Gupta
Enzyme and Microbial Technology.2018; 116: 23. CrossRef
γ-Glutamyl Cysteine Ligase of Lactobacillus reuteri Synthesizes γ-Glutamyl Dipeptides in Sourdough
Bowen Yan, Yuan Yao Chen, Weilan Wang, Jianxin Zhao, Wei Chen, Michael Gänzle
Journal of Agricultural and Food Chemistry.2018; 66(46): 12368. CrossRef
Inference of sigma factor controlled networks by using numerical modeling applied to microarray time series data of the germinating prokaryote
E. Strakova, A. Zikova, J. Vohradsky
Nucleic Acids Research.2014; 42(2): 748. CrossRef
The effectiveness of novel bacteriocin derived from Escherichia coli colonized in the fermented pineapple Ananas comosus (L.) Merr. against pathogenic bacteria isolated from aquaculture sites
S. W. Lee, W. Wendy, L. Montira, A. U. Siti Hazirah
Veterinary World.2014; 7(11): 1014. CrossRef

Research Support, Non-U.S. Gov'ts

Identification and Characterization of an Anti-fungi Fusarium oxysporum f. sp. cucumerium Protease from the Bacillus subtilis Strain N7: Yi Luo , Lifei Sun , Zhen Zhu , Wei Ran , Qirong Shen; J. Microbiol. 2013;51(3):359-366. Published online June 28, 2013; DOI: https://doi.org/10.1007/s12275-013-2627-6

56 View
0 Download
11 Crossref

Abstract

A newly discovered alkaline antifungal protease named P6 from Bacillus subtilis N7 was purified and partially characterized. B. subtilis N7 culture filtrates were purified by 30–60% (NH4)2SO4 precipitation, anion-exchange chromatography and gel filtration chromatography. Sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) revealed a single band of 41.38 kDa. Peptide sequence of protease P6 was determined using a 4800 Plus MALDI TOF/TOFTM Analyzer System. Self-Formed Adaptor PCR (SEFA-PCR) was used to amplify the 1,149 bp open read frame of P6. Dimensional structure prediction using Automatic Modeling Mode software showed that the protease P6 consisted of two β-barrel domains. Purified P6 strongly inhibited spore and mycelium growth of Fusarium oxysporum f. sp. cucumerium (FOC) by causing hypha lysis when the concentration was 25 μg/ml. Characterization of the purified protease indicated that it had substrate specificity for gelatin and was highly active at pH 8.0–10.6 and 70°C. The P6 protease was inhibited by EDTA (2 mmol/L), phenyl methyl sulfonyl fluoride (PMSF, 1 mmol/L), Na+, Fe3+, Cu2+, Mg2+ (5 mmol/L each) and H2O2 (2%, v/v). However, protease activity was activated by Ca2+, K+, Mn2+ (5 mmol/L each), mercaptoethanol (2%, v/v) and Tween 80 (1%, v/v). In additon, activity was also affected by organic solvents such as acetone, normal butanol and ethanol, but not hexane (25%, v/v each).

Citations

Citations to this article as recorded by

Mechanisms of Surfactin from Bacillus subtilis SF1 against Fusarium foetens: A Novel Pathogen Inducing Potato Wilt
Lin Liu, Xiaofan Jin, Xiuhua Lu, Lizhong Guo, Peiwei Lu, Hao Yu, Beibei Lv
Journal of Fungi.2023; 9(3): 367. CrossRef
The Extracellular Lipopeptides and Volatile Organic Compounds of Bacillus subtilis DHA41 Display Broad-Spectrum Antifungal Activity against Soil-Borne Phytopathogenic Fungi
Dhabyan Mutar Kareem Al-Mutar, Muhammad Noman, Noor Salih Abduljaleel Alzawar, Hadi Hussein Qasim, Dayong Li, Fengming Song
Journal of Fungi.2023; 9(8): 797. CrossRef
An Overview of Proteases: Production, Downstream Processes and Industrial Applications
Nathiele Contrera Gimenes, Edgar Silveira, Elias Basile Tambourgi
Separation & Purification Reviews.2021; 50(3): 223. CrossRef
Biocontrol of Orchid-pathogenic Mold,Phytophthora palmivora, by Antifungal Proteins fromPseudomonas aeruginosaRS1
Rapeewan Sowanpreecha, Panan Rerngsamran
Mycobiology.2018; 46(2): 129. CrossRef
Microbial Flora Associated with the Halophyte–Salsola imbricate and Its Biotechnical Potential
Fehmida Bibi, Gary A. Strobel, Muhammad I. Naseer, Muhammad Yasir, Ahmed A. Khalaf Al-Ghamdi, Esam I. Azhar
Frontiers in Microbiology.2018;[Epub] CrossRef
Diversity of indigenous endophytic bacteria associated with the roots of Chinese cabbage (Brassica campestris L.) cultivars and their antagonism towards pathogens
Md. Azizul Haque, Han Dae Yun, Kye Man Cho
Journal of Microbiology.2016; 54(5): 353. CrossRef
Isolation and anti- Verticillium dahliae activity from Bacillus axarquiensis TUBP1 protein
Hong Zeng, Rong Chen, Xiaoxia Luo, Jun Tian
Process Biochemistry.2016; 51(10): 1691. CrossRef
Effect of Two Biological Formulations Based onBacillus subtilisandPseudomonas fluorescenson Control ofDidymella applanata, the Causal Agent of Red Raspberry Cane Spur Blight
Margarita Shternshis, Tatyana Shpatova, Anatoly Belyaev
International Journal of Agronomy.2016; 2016: 1. CrossRef
Cucumber Rhizosphere Microbial Community Response to Biocontrol Agent Bacillus subtilis B068150
Lihua Li, Jincai Ma, A. Ibekwe, Qi Wang, Ching-Hong Yang
Agriculture.2015; 6(1): 2. CrossRef
Construction of a heterologous gene expression system in the banana rhizobacterium strain GW-3 and its colonization ability
Yuguang Wang, Qiyu Xia, He Zhang, Xuehua Lu, Jianbo Sun, Xin Zhang
World Journal of Microbiology and Biotechnology.2014; 30(3): 903. CrossRef
A novel antifungal protein of Bacillus subtilis B25
Zhiqiong Tan, Baoying Lin, Rongyi Zhang
SpringerPlus.2013;[Epub] CrossRef

Characterization, Cloning, and Heterologous Expression of a Subtilisin-Like Serine Protease Gene VlPr1 from Verticillium lecanii: Gang Yu , Jin-Liang Liu , Li-Qin Xie , Xue-Liang Wang , Shi-Hong Zhang , Hong-Yu Pan; J. Microbiol. 2012;50(6):939-946. Published online December 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2199-x

49 View
0 Download
12 Scopus

Abstract: The entomopathogenic fungus Verticillium lecanii is a wellknown biocontrol agent. V. lecanii produces subtilisin-like serine protease (Pr1), which is important in the biological control activity of some insect pests by degrading insect cuticles. In this study, a subtilisin-like serine protease gene VlPr1 was cloned from the fungus and the VlPr1 protein was expressed in Escherichia coli. The VlPr1 gene contains an open reading frame (ORF) interrupted by three short introns, and encodes a protein of 379 amino acids. Protein sequence analysis revealed high homology with subtilisin serine proteases. The molecular mass of the protease was 38 kDa, and the serine protease exhibited its maximal activity at 40°C and pH 9.0. Protease activity was also affected by Mg2+ and Ca2+ concentration. The protease showed inhibitory activity against several plant pathogens, especially towards Fusarium moniliforme.

Journal Article

Chitinase Production by Bacillus thuringiensis and Bacillus licheniformis: Their Potential in Antifungal Biocontrol: Eman Zakaria Gomaa; J. Microbiol. 2012;50(1):103-111. Published online February 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1343-y

55 View
0 Download
108 Crossref

Abstract

Thirty bacterial strains were isolated from the rhizosphere of plants collected from Egypt and screened for production of chitinase enzymes. Bacillus thuringiensis NM101-19 and Bacillus licheniformis NM120-17 had the highest chitinolytic activities amongst those investigated. The production of chitinase by B. thuringiensis and B. licheniformis was optimized using colloidal chitin medium amended with 1.5% colloidal chitin, with casein as a nitrogen source, at 30°C after five days of incubation. An enhancement of chitinase production by the two species was observed by addition of sugar substances and dried fungal mats to the colloidal chitin media. The optimal conditions for chitinase activity by B. thuringiensis and B. licheniformis were at 40°C, pH 7.0 and pH 8.0, respectively. Na+, Mg2+, Cu2+, and Ca2+ caused enhancement of enzyme activities whereas they were markedly inhibited by Zn2+, Hg2+, and Ag+. In vitro, B. thuringiensis and B. licheniformis chitinases had potential for cell wall lysis of many phytopathogenic fungi tested. The addition of B. thuringiensis chitinase was more effective than that of B. licheniformis in increasing the germination of soybean seeds infected with various phytopathogenic fungi.

Citations

Citations to this article as recorded by

Antagonism of Bacillus velezensis ZGE166 Against the Pathogenic Fungi Causing Corm Rot Disease in Saffron (Crocus sativus L.)
Yingqiu Guo, Li Tian, Xinyu Zhu, Shu Liu, Lili Wang, Wankui Li
Microbial Ecology.2025;[Epub] CrossRef
Chitinase-binding gene BvCHiB enhances resistance of Arabidopsis to verticillium wilt through JA and SA pathways
Yujing Liu
Highlights in Science, Engineering and Technology.2025; 129: 213. CrossRef
Effect of Pseudomonas protegens EMM-1 Against Rhizopus oryzae in Interactions with Mexican Autochthonous Red Maize
Bruce Manuel Morales-Barron, Violeta Larios-Serrato, Yolanda Elizabeth Morales-García, Verónica Quintero-Hernández, Paulina Estrada-de los Santos, Jesús Muñoz-Rojas
Life.2025; 15(4): 554. CrossRef
Biocontrol Potential of Streptomyces sp. C2-4 against Ceratobasidium cereale Caused Sharp Eyespot in Wheat
Miju Jo, Sunmin An, Da Som Kim, Won-Jae Chi, Sook-Young Park
Research in Plant Disease.2025; 31(1): 125. CrossRef
Process optimisation for improved chitinase production from marine isolate Bacillus haynesii and bioethanol production with Saccharomyces cerevisiae
Vishnupriya Govindaraj, Dinesh Kumar Anandan, Se-Kwon Kim, Ritu Raval, Keyur Raval
Brazilian Journal of Microbiology.2025; 56(2): 835. CrossRef
The Potential of Beneficial Microbes for Sustainable Alternative Approaches to Control Phytopathogenic Diseases
Ramadan Bakr, Ali Abdelmoteleb, Vianey Mendez-Trujillo, Daniel Gonzalez-Mendoza, Omar Hewedy
Microbiology Research.2025; 16(5): 105. CrossRef
Evaluation of Bacillus isolates as a biological control agents against soilborne phytopathogenic fungi
Derya Maral-Gül, Rengin Eltem
International Microbiology.2024; 28(S1): 75. CrossRef
Chitinase: a potent biocatalyst and its diverse applications
Gaytri Mahajan, Vibhuti Sharma, Reena Gupta
Biocatalysis and Biotransformation.2024; 42(2): 85. CrossRef
Bacillus cereus sensu lato antimicrobial arsenal: An overview
Louis Morandini, Simon Caulier, Claude Bragard, Jacques Mahillon
Microbiological Research.2024; 283: 127697. CrossRef
Effective biological control of Lentil (Lens culinaris) Fusarium wilt and plant growth promotion through native Rhizobacteria
Javeria Ayub, Ayesha Tahir, Umer Iqbal, Huma Ayub, Muhammad Zeeshan Hyder, Amna Kiyani, Fauzia Yusuf Hafeez, Muhammad Kashif Ilyas, Abdul Ghafoor, Tayyaba Yasmin
Physiological and Molecular Plant Pathology.2024; 129: 102203. CrossRef
Bacillus endophytic strains control Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici in tomato cv. Perinha
Guilherme Caldieraro Viana, Leonardo Oliveira Médici, Marcia Soares Vidal, José Ivo Baldani
Brazilian Journal of Microbiology.2024; 55(4): 4019. CrossRef
Exploring chitin: novel pathways and structures as promising targets for biopesticides
Malkiet Kaur, Manju Nagpal, Gitika Arora Dhingra, Ankit Rathee
Zeitschrift für Naturforschung C.2024; 79(5-6): 125. CrossRef
Chitinolytic microorganisms for biological control of plant pathogens: A Comprehensive review and meta-analysis
Sristi Das, Suvasri Dutta, Saibal Ghosh, Abhishek Mukherjee
Crop Protection.2024; 185: 106888. CrossRef
Improved Expression of a Thermostable GH18 Bacterial Chitinase in Two Different Escherichia coli Strains and Its Potential Use in Plant Protection and Biocontrol of Phytopathogenic Fungi
Aymen Ezzine, Safa Ben Hadj Mohamed, Sofiane Bezzine, Yosra Aoudi, Mohamed Rabeh Hajlaoui, Laura Baciou, Issam Smaali
Molecular Biotechnology.2024; 66(9): 2635. CrossRef
A highly active Chitinase-A of Serratia ficaria isolated from Pieris brassicae (Lepidoptera: Pieridae)
Merve Almula Bakirdogen, Gozde Busra Eroglu
Crop Protection.2024; 179: 106623. CrossRef
Bacillus genus industrial applications and innovation: First steps towards a circular bioeconomy
Leonardo Wedderhoff Herrmann, Luiz Alberto Junior Letti, Rafaela de Oliveira Penha, Vanete Thomaz Soccol, Cristine Rodrigues, Carlos Ricardo Soccol
Biotechnology Advances.2024; 70: 108300. CrossRef
In Vitro Antagonistic Activity of Plant Growth Promoting Rhizobacteria Against Aggressive Biotypes of the Green Mold
Baran Mis, Kemal Karaca, Rengin Eltem
Journal of Basic Microbiology.2024;[Epub] CrossRef
Flue-cured tobacco intercropping with insectary floral plants improves rhizosphere soil microbial communities and chemical properties of flue-cured tobacco
Jin Zhong, Wenze Pan, Shenglan Jiang, Yanxia Hu, Guangyuan Yang, Ke Zhang, Zhenyuan Xia, Bin Chen
BMC Microbiology.2024;[Epub] CrossRef
Insights into the whole genome sequence of Bacillus thuringiensis NBAIR BtPl, a strain toxic to the melon fruit fly, Zeugodacus cucurbitae
Nekkanti Aarthi, Vinod K. Dubey, Arakalagud N. Shylesha, Aditya Kukreti, Jagadeesh Patil, Keerthi M. Chandrashekara, Kandan Aravindaram, Ruqiya Seegenahalli, Nanditha Shivakumar, Manjunatha Channappa
Current Genetics.2024;[Epub] CrossRef
Marine chitinase AfChi: green defense management against Colletotrichum gloeosporioides and anthracnose
Rajesh K.M., Keyur Raval, Ritu Raval
AMB Express.2024;[Epub] CrossRef
Biocontrol of fungal phytopathogens by Bacillus pumilus
Jakub Dobrzyński, Zuzanna Jakubowska, Iryna Kulkova, Paweł Kowalczyk, Karol Kramkowski
Frontiers in Microbiology.2023;[Epub] CrossRef
PENGARUH pH, SUHU DAN JENIS SUBSTRAT TERHADAP AKTIVITAS KITINASE Bacillus sp. RNT9
Satrio Adil Pamungkas, Indun Dewi Puspita, Ustadi Ustadi
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology.2023; 19(1): 29. CrossRef
The Prospect of Hydrolytic Enzymes from Bacillus Species in the Biological Control of Pests and Diseases in Forest and Fruit Tree Production
Henry B. Ajuna, Hyo-In Lim, Jae-Hyun Moon, Sang-Jae Won, Vantha Choub, Su-In Choi, Ju-Yeol Yun, Young Sang Ahn
International Journal of Molecular Sciences.2023; 24(23): 16889. CrossRef
Characterization of chitinolytic bacteria newly isolated from the termite Microcerotermes sp. and their biocontrol potential against plant pathogenic fungi
Kittipong Chanworawit, Pachara Wangsoonthorn, Pinsurang Deevong
Bioscience, Biotechnology, and Biochemistry.2023; 87(9): 1077. CrossRef
Enhanced production of N-acetyl-glucosaminidase by marine Aeromonas caviae CHZ306 in bioreactor
Flávio Augusto Cardozo, Valker Feitosa, Carlos Miguel Nóbrega Mendonça, Francisco Vitor Santos da Silva, Attilio Converti, Ricardo Pinheiro de Souza Oliveira, Adalberto Pessoa
Brazilian Journal of Microbiology.2023; 54(3): 1533. CrossRef
Gas Chromatography–Mass Spectrometry Profiling of Volatile Metabolites Produced by Some Bacillus spp. and Evaluation of Their Antibacterial and Antibiotic Activities
Moldir Koilybayeva, Zhanserik Shynykul, Gulbaram Ustenova, Krzysztof Waleron, Joanna Jońca, Kamilya Mustafina, Akerke Amirkhanova, Yekaterina Koloskova, Raushan Bayaliyeva, Tamila Akhayeva, Mereke Alimzhanova, Aknur Turgumbayeva, Gulden Kurmangaliyeva, Ai
Molecules.2023; 28(22): 7556. CrossRef
Thermostable Chitinase Producing Bacterium from Ijen Hot Spring – Indonesia: Isolation, Identification, and Characterization
Ruth Chrisnasari, Liony Priscilla Sutanto, Dian Paulina, Alicia Wahjudi, Tjandra Pantjajani, R.H. Setyobudi, A. Yaro, I. Zekker, M. Zahoor, T. Turkadze
E3S Web of Conferences.2023; 374: 00032. CrossRef
Isolation, biochemical characterization, and genome sequencing of two high‐quality genomes of a novel chitinolytic Jeongeupia species
Nathanael D. Arnold, Daniel Garbe, Thomas B. Brück
MicrobiologyOpen.2023;[Epub] CrossRef
Approach for quick exploration of highly effective broad-spectrum biocontrol strains based on PO8 protein inhibition
Mei Gu, Jiayun Fu, Honglin Yan, Xiaofeng Yue, Shancang Zhao, Qi Zhang, Peiwu Li
npj Science of Food.2023;[Epub] CrossRef
Antifungal Activity of Partially Purified Bacterial Chitinase Against Alternaria alternata
Neslihan Dikbaş, Sevda Uçar, Elif Tozlu, Merve Şenol Kotan, Recep Kotan
Erwerbs-Obstbau.2023; 65(4): 761. CrossRef
Characterization of the chemical fungicides-responsive and bacterial pathogen-preventing Bacillus licheniformis in rice spikelet
Chengfang Zhan, Mengchen Wu, Hongda Fang, Xiaoyu Liu, Jiuyue Pan, Xiaoyan Fan, Mengcen Wang, Haruna Matsumoto
Food Quality and Safety.2023;[Epub] CrossRef
Macrolactin A mediated biocontrol of Fusarium oxysporum and Rhizoctonia solani infestation on Amaranthus hypochondriacus by Bacillus subtilis BS-58
Chitra Pandey, Deepti Prabha, Yogesh Kumar Negi, Dinesh Kumar Maheshwari, Shrivardhan Dheeman, Monika Gupta
Frontiers in Microbiology.2023;[Epub] CrossRef
Formation of recombinant bifunctional fusion protein: A newer approach to combine the activities of two enzymes in a single protein
Patel Nilpa, Kapadia Chintan, R. Z. Sayyed, Hesham El Enshasy, Hala El Adawi, Alaa Alhazmi, Atiah H. Almalki, Shafiul Haque, Rahul Datta
PLOS ONE.2022; 17(4): e0265969. CrossRef
Efficiency and mechanisms of action of pelletized compost loaded with Bacillus velezensis CE 100 for controlling tomato Fusarium wilt
Seo Hyun Hwang, Chaw Ei Htwe Maung, Jun Su Noh, Woon Seon Baek, Jeong-Yong Cho, Kil Yong Kim
Biological Control.2022; 176: 105088. CrossRef
Purification, characterization, and antifungal activity of Bacillus cereus strain NK91 chitinase from rhizospheric soil samples of Himachal Pradesh, India
Nirja Thakur, Amarjit K Nath, Anjali Chauhan, Rakesh Gupta
Biotechnology and Applied Biochemistry.2022; 69(5): 1830. CrossRef
Macrolactin a Mediated Biocontrol of Two Important Fungal Pathogens of Amaranthus Hypochondriacus by Bacillus Subtilis BS-58
Chitra Pandey, Deepti Prabha, Yogesh Kumar Negi, D. K. Maheshwari
SSRN Electronic Journal.2022;[Epub] CrossRef
Morphological and structural characterization of chitin as a substrate for the screening, production, and molecular characterization of chitinase by Bacillus velezensis
Digvijay Dahiya, Akhil Pilli, Pratap Raja Reddy Chirra, Vinay Sreeramula, Nitish Venkateswarlu Mogili, Seenivasan Ayothiraman
Environmental Science and Pollution Research.2022; 29(57): 86550. CrossRef
Recent Advances and Technologies in Chitinase Production Under Solid-State Fermentation
Mini K. Paul, Umesh B.T, Jyothis Mathew
Biosciences Biotechnology Research Asia.2022; 19(4): 815. CrossRef
Effect of soil management systems on the rhizosphere bacterial community structure of tobacco: Continuous cropping vs. paddy-upland rotation
Peng Wang, Shen Yan, Wenshui Zhang, Xiaodan Xie, Mingjie Li, Tianbao Ren, Li Gu, Zhongyi Zhang
Frontiers in Plant Science.2022;[Epub] CrossRef
Broad-spectrum resistance mechanism of serine protease Sp1 in Bacillus licheniformis W10 via dual comparative transcriptome analysis
Lina Yang, Chun Yan, Shuai Peng, Lili Chen, Junjie Guo, Yihe Lu, Lianwei Li, Zhaolin Ji
Frontiers in Microbiology.2022;[Epub] CrossRef
Isolation of Chitinolytic Bacteria from European Sea Bass Gut Microbiota Fed Diets with Distinct Insect Meals
Fábio Rangel, Rafaela A. Santos, Marta Monteiro, Ana Sofia Lavrador, Laura Gasco, Francesco Gai, Aires Oliva-Teles, Paula Enes, Cláudia R. Serra
Biology.2022; 11(7): 964. CrossRef
Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications
Vikram Poria, Anuj Rana, Arti Kumari, Jasneet Grewal, Kumar Pranaw, Surender Singh
Biology.2021; 10(12): 1319. CrossRef
Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats
Delfini CD, Villegas LB, Martínez MA, Baigorí MD
Current Microbiology.2021; 78(7): 2787. CrossRef
Bacilli as sources of agrobiotechnology: recent advances and future directions
Zerihun T. Dame, Mahfuz Rahman, Tofazzal Islam
Green Chemistry Letters and Reviews.2021; 14(2): 246. CrossRef
A Novel Antidipteran Bacillus thuringiensis Strain: Unusual Cry Toxin Genes in a Highly Dynamic Plasmid Environment
Nancy Fayad, Zakaria Kambris, Laure El Chamy, Jacques Mahillon, Mireille Kallassy Awad, Karyn N. Johnson
Applied and Environmental Microbiology.2021;[Epub] CrossRef
Quantitative single molecule RNA-FISH and RNase-free cell wall digestion in Neurospora crassa
Bradley M. Bartholomai, Amy S. Gladfelter, Jennifer J. Loros, Jay C. Dunlap
Fungal Genetics and Biology.2021; 156: 103615. CrossRef
Antifungal Activity of Bacillus velezensis CE 100 against Anthracnose Disease (Colletotrichum gloeosporioides) and Growth Promotion of Walnut (Juglans regia L.) Trees
Vantha Choub, Henry B. Ajuna, Sang-Jae Won, Jae-Hyun Moon, Su-In Choi, Chaw Ei Htwe Maung, Chul-Woo Kim, Young Sang Ahn
International Journal of Molecular Sciences.2021; 22(19): 10438. CrossRef
Key parameters optimization of chitosan production from Aspergillus terreus using apple waste extract as sole carbon source
Alireza Habibi, Salar Karami, Kambiz Varmira, Malihe Hadadi
Bioprocess and Biosystems Engineering.2021; 44(2): 283. CrossRef
Chitinases production: A robust enzyme and its industrial applications
Rahul Vikram Singh, Krishika Sambyal, Anjali Negi, Shubham Sonwani, Ritika Mahajan
Biocatalysis and Biotransformation.2021; 39(3): 161. CrossRef
Microbial chitinases: properties, enhancement and potential applications
Eman Zakaria Gomaa
Protoplasma.2021; 258(4): 695. CrossRef
Drought Exposed Burkholderia seminalis JRBHU6 Exhibits Antimicrobial Potential Through Pyrazine-1,4-Dione Derivatives Targeting Multiple Bacterial and Fungal Proteins
Jay Kishor Prasad, Priyanka Pandey, Richa Anand, Richa Raghuwanshi
Frontiers in Microbiology.2021;[Epub] CrossRef
Pectin lyase enhances cotton resistance to Verticillium wilt by inducing cell apoptosis of Verticillium dahliae
Jing Zhang, Xinru Yu, Chaojun Zhang, Qiong Zhang, Ying Sun, Heqin Zhu, Canming Tang
Journal of Hazardous Materials.2021; 404: 124029. CrossRef
Bacillus licheniformis PR2 Controls Fungal Diseases and Increases Production of Jujube Fruit under Field Conditions
Jun-Hyeok Kwon, Sang-Jae Won, Jae-Hyun Moon, Uk Lee, Yun-Serk Park, Chaw Ei Htwe Maung, Henry B. Ajuna, Young Sang Ahn
Horticulturae.2021; 7(3): 49. CrossRef
Bacillus subtilis and B. licheniformis Isolated from Heterorhabditis indica Infected Apple Root Borer (Dorysthenes huegelii) Suppresses Nematode Production in Galleria mellonella
Akanksha Upadhyay, Sharad Mohan
Acta Parasitologica.2021; 66(3): 989. CrossRef
Dissecting the Environmental Consequences of Bacillus thuringiensis Application for Natural Ecosystems
Maria E. Belousova, Yury V. Malovichko, Anton E. Shikov, Anton A. Nizhnikov, Kirill S. Antonets
Toxins.2021; 13(5): 355. CrossRef
Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers
Tahira Syed, Muhammad Askari, Zhigang Meng, Yanyan Li, Muhammad Abid, Yunxiao Wei, Sandui Guo, Chengzhen Liang, Rui Zhang
Toxins.2020; 12(8): 522. CrossRef
Comparative bioefficacy of Bacillus and Pseudomonas chitinase against Helopeltis theivora in tea (Camellia sinensis (L.) O.Kuntze
M. Suganthi, S. Arvinth, P. Senthilkumar
Physiology and Molecular Biology of Plants.2020; 26(10): 2053. CrossRef
Curing piglets from diarrhea and preparation of a healthy microbiome with Bacillus treatment for industrial animal breeding
Shousong Yue, Zhentian Li, Fuli Hu, Jean-François Picimbon
Scientific Reports.2020;[Epub] CrossRef
Bacillus Thuringiensis – Nowy PotencjaŁ Aplikacyjny
Aleksandra Gęsicka, Agata Henschke, Zuzanna Barańska, Agnieszka Wolna-Maruwka
Postępy Mikrobiologii - Advancements of Microbiology.2020; 59(4): 357. CrossRef
Bioconversion of Colloidal Chitin Using Novel Chitinase from Glutamicibacter uratoxydans Exhibiting Anti-fungal Potential by Hydrolyzing Chitin Within Fungal Cell Wall
Tayyaba Asif, Urooj Javed, Syeda Bushra Zafar, Asma Ansari, Shah Ali Ul Qader, Afsheen Aman
Waste and Biomass Valorization.2020; 11(8): 4129. CrossRef
Biocontrol potential of chitinases produced by newly isolated Chitinophaga sp. S167
Sonia Sharma, Shiv Kumar, Anjali Khajuria, Puja Ohri, Rajinder Kaur, Ramandeep Kaur
World Journal of Microbiology and Biotechnology.2020;[Epub] CrossRef
Screening of an Alkaline CMCase-Producing Strain and the Optimization of its Fermentation Condition
Junmei Zhou, Lianghong Yin, Chenbin Wu, Sijia Wu, Jidong Lu, Hailing Fang, Yongchang Qian
Current Pharmaceutical Biotechnology.2020; 21(13): 1304. CrossRef
Screening of Bacillus thuringiensis strains to identify new potential biocontrol agents against Sclerotinia sclerotiorum and Plutella xylostella in Brassica campestris L.
Meiling Wang, Lili Geng, Xiaoxiao Sun, Changlong Shu, Fuping Song, Jie Zhang
Biological Control.2020; 145: 104262. CrossRef
Chitinases of Bacillus thuringiensis: Phylogeny, Modular Structure, and Applied Potentials
Sheila A. Martínez-Zavala, Uriel E. Barboza-Pérez, Gustavo Hernández-Guzmán, Dennis K. Bideshi, José E. Barboza-Corona
Frontiers in Microbiology.2020;[Epub] CrossRef
Isolation and Identification of Plant Growth-Promoting Bacteria Highly Effective in Suppressing Root Rot in Fava Beans
Mona M. G. Saad, Mahrous Kandil, Youssef M. M. Mohammed
Current Microbiology.2020; 77(9): 2155. CrossRef
Accelerating the Morphogenetic Cycle of the Viral Vector Aedes aegypti Larvae for Faster Larvicidal Bioassays
José Domingos Fontana, Rafael Lopes Ferreira, Tatiana Zuccolotto, Cibelle de Borba Dallagassa, Leonardo Pellizzari Wielewski, Barbara Maria Santano Chalcoski, Mario Antonio Navarro da Silva, Vinicius Sobrinho Richardi, Jonas Golart, Cynara de Melo Rodoval
BioMed Research International.2020;[Epub] CrossRef
Isolation of a novel rhizobacteria having multiple plant growth promoting traits and antifungal activity against certain phytopathogens
Madhurankhi Goswami, Suresh Deka
Microbiological Research.2020; 240: 126516. CrossRef
Long-term continuously monocropped peanut significantly changed the abundance and composition of soil bacterial communities
Mingna Chen, Hu Liu, Shanlin Yu, Mian Wang, Lijuan Pan, Na Chen, Tong Wang, Xiaoyuan Chi, Binghai Du
PeerJ.2020; 8: e9024. CrossRef
Lipopeptide mediated biocontrol activity of endophytic Bacillus subtilis against fungal phytopathogens
Dibya Jyoti Hazarika, Gunajit Goswami, Trishnamoni Gautom, Assma Parveen, Pompi Das, Madhumita Barooah, Robin Chandra Boro
BMC Microbiology.2019;[Epub] CrossRef
Microbial metabolomics: essential definitions and the importance of cultivation conditions for utilizing Bacillus species as bionematicides
I. Horak, G. Engelbrecht, P.J. Jansen Rensburg, S. Claassens
Journal of Applied Microbiology.2019; 127(2): 326. CrossRef
Chitinases As The Key To The Interaction Between Plants And Microorganisms
Anna Kisiel, Katarzyna Jęckowska
Postępy Mikrobiologii - Advancements of Microbiology.2019; 58(3): 317. CrossRef
Biochemical characterization of chitinase A from Bacillus licheniformis DSM8785 expressed in Pichia pastoris KM71H
Gheorghita Menghiu, Vasile Ostafe, Radivoje Prodanovic, Rainer Fischer, Raluca Ostafe
Protein Expression and Purification.2019; 154: 25. CrossRef
Overview of the Antimicrobial Compounds Produced by Members of the Bacillus subtilis Group
Simon Caulier, Catherine Nannan, Annika Gillis, Florent Licciardi, Claude Bragard, Jacques Mahillon
Frontiers in Microbiology.2019;[Epub] CrossRef
Repertoire of the Bacillus thuringiensis Virulence Factors Unrelated to Major Classes of Protein Toxins and Its Role in Specificity of Host-Pathogen Interactions
Yury V. Malovichko, Anton A. Nizhnikov, Kirill S. Antonets
Toxins.2019; 11(6): 347. CrossRef
Unraveling the Optimal Culture Condition for the Antifungal Activity and IAA Production of Phylloplane Serratia plymuthica
Siti Nur Aisyah, Jefri Maldoni, Irma Sulastri, Weni Suryati, Yuli Marlisa, Lissa Herliana, Lily Syukriani, Renfiyeni Renfiyeni, Jamsari Jamsari
Plant Pathology Journal.2019; 18(1): 31. CrossRef
Inhibitory effect and possible mechanism of a Pseudomonas strain QBA5 against gray mold on tomato leaves and fruits caused by Botrytis cinerea
Pan Gao, Jiaxing Qin, Delong Li, Shanyue Zhou, Ya-Wen He
PLOS ONE.2018; 13(1): e0190932. CrossRef
Chitinases—Potential Candidates for Enhanced Plant Resistance towards Fungal Pathogens
Manish Kumar, Amandeep Brar, Monika Yadav, Aakash Chawade, V. Vivekanand, Nidhi Pareek
Agriculture.2018; 8(7): 88. CrossRef
Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host
Nida Toufiq, Bushra Tabassum, Muhammad Umar Bhatti, Anwar Khan, Muhammad Tariq, Naila Shahid, Idrees Ahmad Nasir, Tayyab Husnain
Brazilian Journal of Microbiology.2018; 49(2): 414. CrossRef
Isolation and Evaluation of New Antagonist Bacillus Strains for the Control of Pathogenic and Mycotoxigenic Fungi of Fig Orchards
Özlem Öztopuz, Gülseren Pekin, Ro Dong Park, Rengin Eltem
Applied Biochemistry and Biotechnology.2018; 186(3): 692. CrossRef
Microbial and viral chitinases: Attractive biopesticides for integrated pest management
Francesca Berini, Chen Katz, Nady Gruzdev, Morena Casartelli, Gianluca Tettamanti, Flavia Marinelli
Biotechnology Advances.2018; 36(3): 818. CrossRef
Improvement of Chitinase Production by Bacillus thuringiensis NM101-19 for Antifungal Biocontrol through Physical Mutation
E. Z. Gomaa, O. M. El-Mahdy
Microbiology.2018; 87(4): 472. CrossRef
Bacterial chitinases and their application in biotechnology
Anna Kisiel, Ewa Kępczyńska
Postępy Mikrobiologii - Advancements of Microbiology.2017; 56(3): 306. CrossRef
Combating Fusarium Infection Using Bacillus-Based Antimicrobials
Noor Khan, Maskit Maymon, Ann Hirsch
Microorganisms.2017; 5(4): 75. CrossRef
Screening and Characterisation of Chitinolytic Microorganisms with Potential to Control White Root Disease of Hevea brasiliensis
Nor Afiqah Maiden, Aizat Shamin Noran, Mohd Adi Faiz Ahmad Fauzi, Safiah Atan
Journal of Rubber Research.2017; 20(3): 182. CrossRef
Antifungal Screening of Bioprotective Isolates against Botrytis cinerea, Fusarium pallidoroseum and Fusarium moniliforme
Antoinette De Senna, Amanda Lathrop
Fermentation.2017; 3(4): 53. CrossRef
Simultaneous fermentative chitinase and β-1,3 glucanase production from Streptomyces philanthi RM-1-1-38 and their antifungal activity against rice sheath blight disease
Sawai Boukaew, Wanida Petlamul, Wasana Suyotha, Poonsuk Prasertsan
BioTechnologia.2017; 97(4): 271. CrossRef
Bacillus thuringiensis: a successful insecticide with new environmental features and tidings
Gholamreza Salehi Jouzani, Elena Valijanian, Reza Sharafi
Applied Microbiology and Biotechnology.2017; 101(7): 2691. CrossRef
Enhancement of Exochitinase Production by Bacillus licheniformis AT6 Strain and Improvement of N-Acetylglucosamine Production
Mohamed Amine Aounallah, Imen Ben Slimene-Debez, Kais Djebali, Dorra Gharbi, Majdi Hammami, Sana Azaiez, Ferid Limam, Olfa Tabbene
Applied Biochemistry and Biotechnology.2017; 181(2): 650. CrossRef
Chitinase from Pseudomonas fluorescens and its insecticidal activity against Helopeltis theivora
M. Suganthi, P. Senthilkumar, S. Arvinth, K. N. Chandrashekara
The Journal of General and Applied Microbiology.2017; 63(4): 222. CrossRef
Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria
Eman Zakaria Gomaa
The Journal of General and Applied Microbiology.2017; 63(1): 36. CrossRef
Optimised production of chitinase from a novel mangrove isolate, Bacillus pumilus MCB-7 using response surface methodology
K.S. Rishad, Sharrel Rebello, Vinod Kumar Nathan, S. Shabanamol, M.S. Jisha
Biocatalysis and Agricultural Biotechnology.2016; 5: 143. CrossRef
A new chitinase-D from a plant growth promoting Serratia marcescens GPS5 for enzymatic conversion of chitin
Papa Rao Vaikuntapu, Samudrala Rambabu, Jogi Madhuprakash, Appa Rao Podile
Bioresource Technology.2016; 220: 200. CrossRef
Bacillus thuringiensis C25 which is rich in cell wall degrading enzymes efficiently controls lettuce drop caused by Sclerotinia minor
Anupama Shrestha, Razia Sultana, Jong-Chan Chae, Kangmin Kim, Kui-Jae Lee
European Journal of Plant Pathology.2015; 142(3): 577. CrossRef
Isolation of a Chitinolytic Bacillus licheniformis S213 Strain Exerting a Biological Control Against Phoma medicaginis Infection
Imen Ben Slimene, Olfa Tabbene, Dorra Gharbi, Bacem Mnasri, Jean Marie Schmitter, Maria-Camino Urdaci, Ferid Limam
Applied Biochemistry and Biotechnology.2015; 175(7): 3494. CrossRef
Characterization of regulatory regions involved in the inducible expression of chiB in Bacillus thuringiensis
Chi-Chu Xie, Jin Shi, Hai-Yun Jia, Peng-Fei Li, Yang Luo, Jun Cai, Yue-Hua Chen
Archives of Microbiology.2015; 197(1): 53. CrossRef
YvoA and CcpA Repress the Expression of chiB in Bacillus thuringiensis
Kun Jiang, Li-na Li, Jin-hua Pan, Ting-ting Wang, Yue-hua Chen, Jun Cai, S.-J. Liu
Applied and Environmental Microbiology.2015; 81(19): 6548. CrossRef
Ecology of Bacillaceae
Ines Mandic-Mulec, Polonca Stefanic, Jan Dirk van Elsas, Patrick Eichenberger, Adam Driks
Microbiology Spectrum.2015;[Epub] CrossRef
Chitinase biotechnology: Production, purification, and application
Yuriy Mihaylov Stoykov, Atanas Ivanov Pavlov, Albert Ivanov Krastanov
Engineering in Life Sciences.2015; 15(1): 30. CrossRef
Efficient biosynthesis of a chitinase from Halobacterium salinarum expressed in Escherichia coli
Fatima Moscoso, Myriam Sieira, Alberto Domínguez, Francisco J. Deive, Maria A. Longo, Maria A. Sanromán
Journal of Chemical Technology & Biotechnology.2014; 89(11): 1653. CrossRef
Potential use and mode of action of the new strainBacillus thuringiensisUM96 for the biological control of the grey mould phytopathogenBotrytis cinerea
Sofía Martínez-Absalón, Daniel Rojas-Solís, Rocío Hernández-León, Cristina Prieto-Barajas, Ma. del Carmen Orozco-Mosqueda, Juan José Peña-Cabriales, Shohei Sakuda, Eduardo Valencia-Cantero, Gustavo Santoyo
Biocontrol Science and Technology.2014; 24(12): 1349. CrossRef
Isolation and characterization of an antifungal protein from Bacillus licheniformis HS10
Zhixin Wang, Yunpeng Wang, Li Zheng, Xiaona Yang, Hongxia Liu, Jianhua Guo
Biochemical and Biophysical Research Communications.2014; 454(1): 48. CrossRef
Dual silencing of long and short Amblyomma americanum acidic chitinase forms weakens the tick cement cone stability
Tae K. Kim, Jenny Curran, Albert Mulenga
Journal of Experimental Biology.2014;[Epub] CrossRef
Partial Purification of Bacterial Chitinase as Biocontrol of Leaf Blight Disease on Oil Palm
Muhammad Asril, Nisa Rachmania Mubarik, Aris Tri Wahyudi
Research Journal of Microbiology.2014; 9(6): 265. CrossRef
Characterization and evaluation of Staphylococcus sp. strain LZ16 for the biological control of rice blast caused by Magnaporthe oryzae
Qin Yu, Zhu Liu, Derun Lin, Wei Zhang, Qun Sun, Jianqing Zhu, Min Lin
Biological Control.2013; 65(3): 338. CrossRef
Development of an Industrial Microbial System for Chitinolytic Enzymes Production
F. Moscoso, L. Ferreira, M.A. Fernández de Dios, F.J. Deive, M.A. Longo, M.A. Sanromán
Industrial & Engineering Chemistry Research.2013; 52(30): 10046. CrossRef
Bacillus thuringiensiscolonises plant roots in a phylogeny-dependent manner
J. Cristian Vidal-Quist, Hilary J. Rogers, Eshwar Mahenthiralingam, Colin Berry
FEMS Microbiology Ecology.2013; 86(3): 474. CrossRef
Comparative Genome Analysis of Enterobacter cloacae
Wing-Yee Liu, Chi-Fat Wong, Karl Ming-Kar Chung, Jing-Wei Jiang, Frederick Chi-Ching Leung, Jingfa Xiao
PLoS ONE.2013; 8(9): e74487. CrossRef
Antifungal activity of the lipopeptides produced by Bacillus amyloliquefaciens anti-CA against Candida albicans isolated from clinic
Bo Song, Yan-Jun Rong, Ming-Xin Zhao, Zhen-Ming Chi
Applied Microbiology and Biotechnology.2013; 97(16): 7141. CrossRef

Research Support, Non-U.S. Gov'ts

NOTE] Complete Sequence and Organization of the Sphingobium chungbukense DJ77 pSY2 Plasmid: Sun-Mi Yeon , Young-Chang Kim; J. Microbiol. 2011;49(4):684-688. Published online September 2, 2011; DOI: https://doi.org/10.1007/s12275-011-1262-3

37 View
0 Download
3 Crossref

Abstract

Sphingobium chungbukense DJ77 is capable of metabolizing priority chemicals of human health concern such as polycyclic aromatic hydrocarbons (PAHs), extracellular polysaccharide (EPS), and antibiotics. Here, we report the complete DNA and genetic organization of the plasmid pSY2 from strain DJ77. A DNA sequence analysis revealed that pSY2 comprises 18,779 bp encoding 22 open reading frames (ORFs) with 59.5% G+C content. The ORFs on pSY2 were classified into DNA replication, conjugative function, transposition, plasmid stability/partition, and other functional groups (transport, fatty acid biosynthesis, stress, and growth rate regulation). Three ORFs on pSY2 were hypothetical proteins.

Citations

Citations to this article as recorded by

Biochemical characterization and molecular docking analysis of novel esterases from Sphingobium chungbukense DJ77
Woo-Ri Shin, Hyun-Ju Um, Young-Chang Kim, Sun Chang Kim, Byung-Kwan Cho, Ji-Young Ahn, Jiho Min, Yang-Hoon Kim
International Journal of Biological Macromolecules.2021; 168: 403. CrossRef
Molecular Docking Analysis and Biochemical Evaluation of Levansucrase from Sphingobium chungbukense DJ77
Soo Youn Lee, Woo-Ri Shin, Simranjeet Singh Sekhon, Jin-Pyo Lee, Young-Chang Kim, Ji-Young Ahn, Yang-Hoon Kim
ACS Combinatorial Science.2018; 20(7): 414. CrossRef
Degradative plasmids from sphingomonads
Andreas Stolz
FEMS Microbiology Letters.2014; 350(1): 9. CrossRef

Isolation and Characterization of Ethylbenzene Degrading Pseudomonas putida E41: Lan-Hee Kim , Sang-Seob Lee; J. Microbiol. 2011;49(4):575-584. Published online September 2, 2011; DOI: https://doi.org/10.1007/s12275-011-0399-4

57 View
0 Download
7 Scopus

Abstract: Pseudomonas putida E41 was isolated from oil-contaminated soil and showed its ability to grow on ethylbenzene as the sole carbon and energy source. Moreover, P. putida E41 show the activity of biodegradation of ethylbenzene in the batch culture. E41 showed high efficiency of biodegradation of ethylbenzene with the optimum conditions (a cell concentration of 0.1 g wet cell weight/L, pH 7.0, 25°C, and ethylbenzene concentration of 50 mg/L) from the results of the batch culture. The maximum degradation rate and specific growth rate (μmax) under the optimum conditions were 0.19±0.03 mg/mg-DCW (Dry Cell Weight)/h and 0.87±0.13 h-1, respectively. Benzene, toluene and ethylbenzene were degraded when these compounds were provided together; however, xylene isomers persisted during degradation by P. putida E41. When using a bioreactor batch system with a binary culture with P. putida BJ10, which was isolated previously in our lab, the degradation rate for benzene and toluene was improved in BTE mixed medium (each initial concentration: 50 mg/L). Almost all of the BTE was degraded within 4 h and 70-80% of m-, p-, and o-xylenes within 11 h in a BTEX mixture (initial concentration: 50 mg/L each). In summary, we found a valuable new strain of P. putida, determined the optimal degradation conditions for this isolate and tested a mixed culture of E41 and BJ10 for its ability to degrade a common sample of mixed contaminants containing benzene, toluene, and xylene.

Purification and Partial Characterization of a Detergent and Oxidizing Agent Stable Alkaline Protease from a Newly Isolated Bacillus subtilis VSG-4 of Tropical Soil: Sib Sankar Giri , V. Sukumaran , Shib Sankar Sen , M. Oviya , B. Nazeema Banu , Prasant Kumar Jena; J. Microbiol. 2011;49(3):455-461. Published online June 30, 2011; DOI: https://doi.org/10.1007/s12275-011-0427-4

40 View
0 Download
31 Scopus

Abstract: An extracellular detergent tolerant protease producing strain VSG-4 was isolated from tropical soil sample and identified as Bacillus subtilis based on morphological, biochemical characteristics as well as 16S-rRNA gene sequencing. The VSG-4 protease was purified to homogeneity using ammonium sulphate precipitation, dialysis and sephadex G-200 gel permeation chromatography with a 17.4 purification fold. The purified enzyme was active and stable over a broad range of pH (8.0-11.0, optimum at 9.0) and temperature (40°C to 60°C, optimum at 50°C). The thermostability of the enzyme was significantly increased by the addition CaCl2. This enzyme was strongly inhibited by PMSF and DFP, suggesting that it belongs to the serine protease superfamily. The purified VSG-4 alkaline protease showed remarkable stability in anionic (5 mM SDS) and ionic (1% Trion X-100 and 1% Tween 80) detergents. It retained 97±2% and 83.6±1.1% of its initial activity after 1 h preincubation in the presence of 1% H2O2 and 1% sodium perborate, respectively. Furthermore, the purified enzyme showed excellent stability and compatibility with some commercial laundry detergents besides its stain removal capacity. Considering these promising properties, VSG-4 protease may find tremendous application in laundry detergent formulations.

A Thermostable Phytase from Neosartorya spinosa BCC 41923 and Its Expression in Pichia pastoris: Patcharaporn Pandee , Pijug Summpunn , Suthep Wiyakrutta , Duangnate Isarangkul , Vithaya Meevootisom; J. Microbiol. 2011;49(2):257-264. Published online May 3, 2011; DOI: https://doi.org/10.1007/s12275-011-0369-x

52 View
0 Download
19 Scopus

Abstract: A phytase gene was cloned from Neosartorya spinosa BCC 41923. The gene was 1,455 bp in size, and the mature protein contained a polypeptide of 439 amino acids. The deduced amino acid sequence contains the consensus motif (RHGXRXP) which is conserved among phytases and acid phosphatases. Five possible disulfide bonds and seven potential N-glycosylation sites have been predicted. The gene was expressed in Pichia pastoris KM71 as an extracellular enzyme. The purified enzyme had specific activity of 30.95 U/mg at 37°C and 38.62 U/mg at 42°C. Molecular weight of the deglycosylated recombinant phytase, determined by SDS-PAGE, was approximately 52 kDa. The optimum pH and temperature for activity were pH 5.5 and 50°C. The residual phytase activity remained over 80% of initial activity after the enzyme was stored in pH 3.0 to 7.0 for 1 h, and at 60% of initial activity after heating at 90°C for 20 min. The enzyme exhibited broad substrate specificity, with phytic acid as the most preferred substrate. Its Km and Vmax for sodium phytate were 1.39 mM and 434.78 U/mg, respectively. The enzyme was highly resistant to most metal ions tested, including Fe2+, Fe3+, and Al3+. When incubated with pepsin at a pepsin/phytase ratio of 0.02 (U/U) at 37°C for 2 h, 92% of its initial activity was retained. However, the enzyme was very sensitive to trypsin, as 5% of its initial activity was recovered after treating with trypsin at a trypsin/phytase ratio of 0.01 (U/U).

Characterization, Gene Cloning, and Heterologous Expression of β-Mannanase from a Thermophilic Bacillus subtilis: Pijug Summpunn , Suttidarak Chaijan , Duangnate Isarangkul , Suthep Wiyakrutta , Vithaya Meevootisom; J. Microbiol. 2011;49(1):86-93. Published online March 3, 2011; DOI: https://doi.org/10.1007/s12275-011-0357-1

65 View
0 Download
30 Crossref

Abstract

Bacillus subtilis BCC41051 producing a thermostable β-mannanase was isolated from soybean meal-enriched soil and was unexpectedly found to be thermophilic in nature. The extracellular β-mannanase (ManA) produced was hydrophilic, as it was not precipitated even with ammonium sulfate at 80% saturation. The estimated molecular weight of ManA was 38.0 kDa by SDS-PAGE with a pI value of 5.3. Optimal pH and temperature for mannan-hydrolyzing activity was 7.0 and 60°C, respectively. The enzyme was stable over a pH range of 5.0-11.5, and at temperatures of up to 60°C for 30 min, with more than 80% of its activity retained. ManA was strongly inhibited by Hg2+ (1 mM), but was sensitive to other divalent ions to a lesser degree. The gene of ManA encoded a protein of 362 amino acid residues, with the first 26 residues identified as a signal peptide. High expression of recombinant ManA was achieved in both Escherichia coli BL21 (DE3) (415.18 U/ml) and B. megaterium UNcat (359 U/ml).

Citations

Citations to this article as recorded by

A thermostable and acidophilic mannanase from Bacillus mojavensis: its sustainable production using spent coffee grounds, characterization, and application in grape juice processing
Sümeyye Cilmeli, Tuğrul Doruk, Serpil Könen-Adıgüzel, Ali Osman Adıgüzel
Biomass Conversion and Biorefinery.2024; 14(3): 3811. CrossRef
Production and purification of high activity β-mannanase from Streptomyces rochei and enzymatic hydrolysis of sugar palm fruit for galactomannan oligosaccharide (GMOS) production
Annisyia Zarina Putri, Nanik Rahmani, Rike Rachmayati, Eva Agustriana, Siti Eka Yulianti, Hans Wijaya, Nuryati, Akhirta Atikana, Shanti Ratnakomala, Puspita Lisdiyanti, Yopi, Bambang Prasetya
Bioresource Technology Reports.2024; 25: 101744. CrossRef
Effects of Signal Peptide and Chaperone Co-Expression on Heterologous Protein Production in Escherichia coli
Juntratip Jomrit, Suhardi Suhardi, Pijug Summpunn
Molecules.2023; 28(14): 5594. CrossRef
Comprehensive utilization of palm kernel cake for producing mannose and manno-oligosaccharide mixture and yeast culture
Weiwei Dong, Shengqi Dong, Yongxu Li, Yutian Lei, Nan Peng, Yunxiang Liang, Shumiao Zhao, Xiangyang Ge
Applied Microbiology and Biotechnology.2022; 106(3): 1045. CrossRef
Enhanced extracellular β‐mannanase production by overexpressing PrsA lipoprotein in Bacillus subtilis and optimizing culture conditions
Liyu Xu, Yongyong Zhang, Yuehan Dong, Gang Qin, Xiao Zhao, Yanyan Shen
Journal of Basic Microbiology.2022; 62(7): 815. CrossRef
Expression, Characterization and Structure Analysis of a New GH26 Endo-β-1, 4-Mannanase (Man26E) from Enterobacter aerogenes B19
Huijing Liu, Jie Liu, Tangbing Cui
Applied Sciences.2020; 10(21): 7584. CrossRef
Galactomannan degradation by thermophilic enzymes: a hot topic for biotechnological applications
Martina Aulitto, Salvatore Fusco, Danila Limauro, Gabriella Fiorentino, Simonetta Bartolucci, Patrizia Contursi
World Journal of Microbiology and Biotechnology.2019;[Epub] CrossRef
Characterization and high-efficiency secreted expression in Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain S10
Cheng Zhou, Yanfen Xue, Yanhe Ma
Microbial Cell Factories.2018;[Epub] CrossRef
Purification of β -mannanase derived from Bacillus subtilis ATCC 11774 using ionic liquid as adjuvant in aqueous two-phase system
Nur Fazrin Husna Abdul Aziz, Sahar Abbasiliasi, Hui Suan Ng, Pongsathon Phapugrangkul, Mohamad Hafizi Abu Bakar, Yew Joon Tam, Joo Shun Tan
Journal of Chromatography B.2017; 1055-1056: 104. CrossRef
Production, properties, and applications of endo-β-mannanases
Praveen Kumar Srivastava, Mukesh Kapoor
Biotechnology Advances.2017; 35(1): 1. CrossRef
A novel expression vector for the secretion of abaecin in Bacillus subtilis
Li Li, Lan Mu, Xiaojuan Wang, Jingfeng Yu, Ruiping Hu, Zhen Li
Brazilian Journal of Microbiology.2017; 48(4): 809. CrossRef
Purification, characterization, and overexpression of an endo-1,4-β-mannanase from thermotolerant Bacillus sp. SWU60
Weeranuch Seesom, Polphet Thongket, Tomohiro Yamamoto, Shigeo Takenaka, Tatsuji Sakamoto, Wasana Sukhumsirichart
World Journal of Microbiology and Biotechnology.2017;[Epub] CrossRef
Gene cloning, expression, and characterization of a novel β‐mannanase from the endophyte Paenibacillus sp. CH‐3
Jian‐Xin Zhang, Ze‐Tian Chen, Xiao‐Lin Meng, Guang‐Ya Mu, Wen‐Bo Hu, Jie Zhao, Guo‐Xing Nie
Biotechnology and Applied Biochemistry.2017; 64(4): 471. CrossRef
A Recombinant Highly Thermostable β-Mannanase (ReTMan26) from Thermophilic Bacillus subtilis (TBS2) Expressed in Pichia pastoris and Its pH and Temperature Stability
Zhangcai Luo, Jing Miao, Guoying Li, Yao Du, Xiaobin Yu
Applied Biochemistry and Biotechnology.2017; 182(4): 1259. CrossRef
Production of Extracellular β-mannanase by Bacillus amyloliquefaciens on a Coconut Waste Substrate
Zurmiati ., Wizna ., M. Hafil Abbas, Maria Endo Mahata
Pakistan Journal of Nutrition.2017; 16(9): 700. CrossRef
Bio-chemical characterization of a β-mannanase from Bacillus licheniformis HDYM-04 isolated from flax water-retting liquid and its decolorization ability of dyes
J. P. Ge, R. P. Du, D. Zhao, G. Song, M. Jin, W. X. Ping
RSC Advances.2016; 6(28): 23612. CrossRef
Molecular cloning of kman coding for mannanase from Klebsiella oxytoca KUB-CW2-3 and its hybrid mannanase characters
Nawapan Pongsapipatana, Piyanat Damrongteerapap, Sudathip Chantorn, Wilawan Sintuprapa, Suttipun Keawsompong, Sunee Nitisinprasert
Enzyme and Microbial Technology.2016; 89: 39. CrossRef
Production optimization and functional characterization of thermostable β-mannanase from Malbranchea cinnamomea NFCCI 3724 and its applicability in mannotetraose (M4) generation
Saroj Ahirwar, Hemant Soni, Hemant Kumar Rawat, Mohd. Anis Ganaie, Kumar Pranaw, Naveen Kango
Journal of the Taiwan Institute of Chemical Engineers.2016; 63: 344. CrossRef
Purification and Characterization of a Thermostableβ-Mannanase fromBacillus subtilisBE-91: Potential Application in Inflammatory Diseases
Lifeng Cheng, Shengwen Duan, Xiangyuan Feng, Ke Zheng, Qi Yang, Zhengchu Liu
BioMed Research International.2016; 2016: 1. CrossRef
Degradation of konjac glucomannan by Thermobifida fusca thermostable β-mannanase from yeast transformant
Cheng-Yu Chen, Yu-Chun Huang, Ting-Ya Yang, Jhen-Yi Jian, Wei-Lin Chen, Chao-Hsun Yang
International Journal of Biological Macromolecules.2016; 82: 1. CrossRef
Identification of the C-Terminal GH5 Domain from CbCel9B/Man5A as the First Glycoside Hydrolase with Thermal Activation Property from a Multimodular Bifunctional Enzyme
Rong Wang, Li Gong, Xianli Xue, Xing Qin, Rui Ma, Huiying Luo, Yongjie Zhang, Bin Yao, Xiaoyun Su, Israel Silman
PLOS ONE.2016; 11(6): e0156802. CrossRef
Molecular and biochemical characterizations of a new low-temperature active mannanase
Rui Zhang, Junpei Zhou, Yajie Gao, Yaping Guan, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Zunxi Huang
Folia Microbiologica.2015; 60(6): 483. CrossRef
Secretory expression and characterization of a novel thermo-stable, salt-tolerant endo-1,4-β-mannanase of Bacillus subtilis WD23 by Pichia pastoris
Huiling Li, Zuyan Liu, Chunlei Wang, Shichen Huang, Min Zhao
European Food Research and Technology.2015; 240(4): 671. CrossRef
Production and Properties of Mannanase by a Bacillus amyloliquefaciens Isolate
Ki-Hong Yoon
The Korean Journal of Microbiology.2014; 50(2): 158. CrossRef
Purification and characterization of an alkali-thermostable β-mannanase from Bacillus nealsonii PN-11 and its application in mannooligosaccharides preparation having prebiotic potential
Prakram Singh Chauhan, Prince Sharma, Neena Puri, Naveen Gupta
European Food Research and Technology.2014; 238(6): 927. CrossRef
Comparison of Acidic pH and Temperature Stabilities between Two Bacillus Mannanases Produced from Recombinant Escherichia coli
Ho Jin Jeon, Ki-Hong Yoon
The Korean Journal of Microbiology.2014; 50(4): 327. CrossRef
Insight into microbial hemicellulases other than xylanases: a review
Veeresh Juturu, Jin Chuan Wu
Journal of Chemical Technology & Biotechnology.2013; 88(3): 353. CrossRef
Cloning, Expression, and Characterization of β-mannanase from Bacillus subtilis MAFIC-S11 in Pichia pastoris
Junnan Lv, Yiqun Chen, Honglei Pei, Wenhan Yang, Zhimin Li, Bing Dong, Yunhe Cao
Applied Biochemistry and Biotechnology.2013; 169(8): 2326. CrossRef
Novel low-temperature-active, salt-tolerant and proteases-resistant endo-1,4-β-mannanase from a new Sphingomonas strain
Junpei Zhou, Rui Zhang, Yajie Gao, Junjun Li, Xianghua Tang, Yuelin Mu, Feng Wang, Chao Li, Yanyan Dong, Zunxi Huang
Journal of Bioscience and Bioengineering.2012; 113(5): 568. CrossRef
Mannanases: microbial sources, production, properties and potential biotechnological applications
Prakram Singh Chauhan, Neena Puri, Prince Sharma, Naveen Gupta
Applied Microbiology and Biotechnology.2012; 93(5): 1817. CrossRef

Characterization of Plasmid pSY3 in Sphingobium chungbukense DJ77: Sun-Mi Yeon , Young-Chang Kim; J. Microbiol. 2009;47(6):796-800. Published online February 4, 2010; DOI: https://doi.org/10.1007/s12275-009-0329-x

35 View
0 Download
4 Scopus

Abstract: This study determined the complete nucleotide sequence of the plasmid pSY3 from Sphingobium chungbukense DJ77. It was 35,735 bp long with a G+C content of 61.9%. Forty open reading frames (ORFs) were found. We predicted these ORFs would encode proteins associated with plasmid replication, conjugative transfer, transposition of genes, plasmid stability/partition, hypothetical protein, and some other functions. Genes for biodegradation were not found. No other plasmid homologous to pSY3 in the overall nucleotide sequence or gene organization could be found in the NCBI database.

Journal Article

Characterization of the Bacillus subtilis WL-3 Mannanase from a Recombinant Escherichia coli: Ki-Hong Yoon , Seesub Chung , Byung-Lak Lim; J. Microbiol. 2008;46(3):344-349. Published online July 5, 2008; DOI: https://doi.org/10.1007/s12275-008-0045-y

45 View
0 Download
31 Scopus

Abstract: A mannanase was purified from a cell-free extract of the recombinant Escherichia coli carrying a Bacillus subtilis WL-3 mannanase gene. The molecular mass of the purified mannanase was 38 kDa as estimated by SDS-PAGE. Optimal conditions for the purified enzyme occurred at pH 6.0 and 60°C. The specific activity of the purified mannanase was 5,900 U/mg on locust bean gum (LBG) galactomannan at pH 6.0 and 50°C. The activity of the enzyme was slightly inhibited by Mg2+, Ca2+, EDTA and SDS, and noticeably enhanced by Fe2+. When the enzyme was incubated at 4°C for one day in the presence of 3 mM Fe2+, no residual activity of the mannanase was observed. The enzyme showed higher activity on LBG and konjac glucomannan than on guar gum galactomannan. Furthermore, it could hydrolyze xylans such as arabinoxylan, birchwood xylan and oat spelt xylan, while it did not exhibit any activities towards carboxymethylcellulose and para-nitrophenyl-β-mannopyranoside. The predominant products resulting from the mannanase hydrolysis were mannose, mannobiose and mannotriose for LBG or mannooligosaccharides including mannotriose, mannotetraose, mannopentaose and mannohexaose. The enzyme could hydrolyze mannooligosaccharides larger than mannobiose.

Research Support, Non-U.S. Gov't

Strain Improvement of Candida tropicalis for the Production of Xylitol:Biochemical and Physiological Characterization of Wild-type and Mutant Strain CT-OMV5: Ravella Sreenivas Rao , Cherukuri Pavana Jyothi , Reddy Shetty Prakasham , Chaganti Subba Rao , Ponnupalli Nageshwara Sarma , Linga Venkateswar Rao; J. Microbiol. 2006;44(1):113-120.; DOI: https://doi.org/2328 [pii]

40 View
0 Download

Abstract: Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81g of xylitol per gram of xylose. This was further mutated with N-methyl-N’-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea. The CT-OMV5 mutant strain differs by 12 tests when compared to the wild-type Candida tropicalis strain. The XR activity was higher in mutant CT-OMV5. The distinct difference between the mutant and wild-type strain is the presence of numerous chlamydospores in the mutant. In this investigation, we have demonstrated that mutagenesis was successful in generating a superior xylitol-producing strain, CT-OMV5, and uncovered distinctive biochemical and physiological characteristics of the wild-type and mutant strain, CT-OMV5.

Purification and characterization of a xylanase from alkalophilic cephalosporium sp. RYM-202: Kang, Myoung Kyu , Kwon, Tae Ik , Yang, Young Ki , Rhee, Young Ha; J. Microbiol. 1995;33(2):109-114.

59 View
0 Download

Abstract: Alkalophilic Cephalosporium sp. RYM-202 produced multiple xylanases extracellularly. One of these xylanases was purified to electrophoretical homogeneity by chromatography with DEAE-Sephadex A-50, Sephacryl S-200 HR and Superose 12 HR. The purified xylanase differed from most other microbial xylanases in that it had low-molecular weight and acidic isoelectric point. The molecular weight of the xylanase in that it had low-molecular weight and acidic isoelectric point. The molecular weight of the xylanase was 23 kDa by SDS-polyacrylamide electrophoresis and 24 kDa by gel permeation chromatography, and the isoelectric point was 4.3. The xylanase had the highest activity permentation chromatography, and the isoelectric point was 4.3. The xylanase had the highest activity permeation chromatography, and the isoelectric point was 4.3. The xylanase had the highest activity at pH 8.0 and 50℃. It was stable over a wide range of pH and retained more than 80% of its original activity after 24 h of incubation even at pH 12. The Km values of this enzyme on birchwood xylan and oat spelts xylan were 2.33 and 3.45 mg/ml, respectively. The complete inhibition of the enzyme of n-bromosuccinimide suggests the involvement of tryptophan in the active site. The sylanase lacked activity towards crystalline cellulose and carboxymethyl cellulose.

Purification and Characterization of an Extracellular Protease from Culture Filtrate of Salmonella schttmulleri: Na, Byoung Kuk , Song, Chul Yong; J. Microbiol. 1995;33(3):244-251.

50 View
0 Download

Abstract: An extracellular protease of Salmonella schottmulleri was purified from culture filtrate by using 0-75% ammonium sulfate precipitation, DEAE Sepharose Fast Flow ion exchange chromatography, Ultrogel HA chromatography and Sephacryl S-200 HR molecular sieve chromatography. To measure enzyme activity, synthetic dipeptide substrate (CBZ-arg-arg-AFC) with low molecular weight was employed as substrate. The molecular weight of the purified enzyme was approximately 80 kDa when determined by gel filtration on Sephacryl S-200 HR and 73 kDa when estimated by SDS-PAGE. The isoelectric point was 5.45. The activity of the purified enzyme was inhibited by metal chelating agesnts such as EDTA and 1.10-phenanthroline. The divalent cations, such as Ca^2+, Zn^2+, Fe^2+, Mg^2+ enhanced its activity. These results suggested that it was a metalloprotease. It had a narrow pH optimum of 6.5-7.5 with a maximum at pH 7.0 and a temperature optimum of 40℃. It was stable at least for 1 week at 40℃ and maintained its activity for 24 hours at 50℃, but it was rapidly inactivated at 65℃. This protease was shown to be sensitive to sodium 50℃, but it was rapidly inactivated at 65℃. This protease was shown to be sensitive to sodium 50℃, but it was rapidly inactivated at 65℃. This protease was shown to be sensitive to sodium 50℃, but it was rapidly inactivated at 65℃. This protease was shown to be sensitive to sodium dodecyl sulfate (SDS) and was inactivated in a dose-dependent manner. However, it was resistant to Triton X-100 and the activity was enhanced to 32.3% with treatment of 0.025% Triton X-100.

Partial characterization of proteases from culture filtrate of mycobacterium tuberculosis: Na, Byoung Kuk , Song, Chul Yong , Park, Young Kil , Bai, Gill Han , Ki, Sang Jae; J. Microbiol. 1996;34(2):198-205.

50 View
0 Download

Abstract: Two proteases were partially characterized from culture filtrate of Mycobacterium, tuberculosis KIT110. Their molecular weights were approximately 200 and 180 kDa, respectively and they exhibited similar enzymatic characteristics. These enzymes were inhibited significantly by EDTA and to some extent by EGTA. Their activity was enhanced by Ca^2+ and Mg^2+ to some degree. However, Cu^2+ and Ag^2+ completely inhibited the enzyme activity at the concentration of 2.5 and 5 mM, respectively. The optimal pH was 7.0 and optimal temperature was around 40℃. These enzymes were rapidly inactivated at 80℃. Therefore, they were heat-labile, neutral metalloproteases. These enzymes exhibited antigenicity shown by their reacting with sera from the partients with pulmonary tuberculosis. These enzymes were able to degrade serum proteins including hemoglobin, bovine serum albumin, lysozyme and immunoglobulin G and structural matrix protein such as type I collagen. Therefore, these enzymes may be thought to contribute to tissue necrosis and pathogenesis during infection.

First
Prev
Page of 2
Next
Last

Search