Journal of Microbiology

Research Support, Non-U.S. Gov't

Functional Characterization of Extracellular Chitinase Encoded by the YlCTS1 Gene in a Dimorphic Yeast Yarrowia lipolytica: Jeong-Nam Park , Chang Pyo Han , Dong-Jik Lee , Seon Ah Cheon , Hyun Ah Kang; J. Microbiol. 2014;52(4):284-291. Published online March 29, 2014; DOI: https://doi.org/10.1007/s12275-014-4070-8

73 View
0 Download
4 Crossref

The hemiascomycetes yeast Yarrowia lipolytica is a dimorphic yeast with alternating yeast and mycelia forms. Bioinformatic analysis revealed the presence of three putative chitinase genes, YlCTS1, YlCTS2, and YlCTS3, in the Y. lipolytica genome. Here, we demonstrated that the protein of YlCTS1 (YlCts1p), which contains an N-terminal secretion signal peptide, a long C-terminal Ser/Thr-rich domain, and a chitin-binding domain, is a homologue to Saccharomyces cerevisiae chitinase 1 (ScCts1p). Deletion of YlCTS1 remarkably reduced extracellular endochitinase activity in the culture supernatant of Y. lipolytica and enhanced cell aggregation, suggesting a role of YlCts1p in cell separation as ScCts1p does in S. cerevisiae. However, loss of YlCts1p function did not affect hyphal formation induced by fetal bovine serum addition. The mass of YlCts1p was dramatically decreased by jack bean α-mannosidase digestion but not by PNGase F treatment, indicating that YlCts1p is modified only by Omannosylation without N-glycosylation. Moreover, the O-glycan profile of YlCts1p was identical to that of total cell wall mannoproteins, supporting the notion that YlCts1p can be used as a good model for studying O-glycosylation in this dimorphic yeast.

Citations

Citations to this article as recorded by

Advancing Ultrasensitive, Drift-Correcting Dual Floating Gate Organic Electrochemical Transistors for Yeast Sensing
Jonathan Harris, Michael Brothers, Victoria Coyle, Steve Kim, Erin Ratcliff
Chemistry of Materials.2024; 36(1): 324. CrossRef
The N-Acetylglucosamine Kinase from Yarrowia lipolytica Is a Moonlighting Protein
Carmen-Lisset Flores, Joaquín Ariño, Carlos Gancedo
International Journal of Molecular Sciences.2021; 22(23): 13109. CrossRef
Recovery and valorization of agri-food wastes and by-products using the non-conventional yeast Yarrowia lipolytica
Davide Gottardi, Lorenzo Siroli, Lucia Vannini, Francesca Patrignani, Rosalba Lanciotti
Trends in Food Science & Technology.2021; 115: 74. CrossRef
Functional analysis of recombinant human and Yarrowia lipolytica O-GlcNAc transferases expressed in Saccharomyces cerevisiae
Hye Ji Oh, Hye Yun Moon, Seon Ah Cheon, Yoonsoo Hahn, Hyun Ah Kang
Journal of Microbiology.2016; 54(10): 667. CrossRef

Journal Articles

Note] Antifungal Chitinase against Human Pathogenic Yeasts from Coprinellus congregatus: Yeeun Yoo Hyoung T. Choi; J. Microbiol. 2014;52(5):441-443. Published online February 17, 2014; DOI: https://doi.org/10.1007/s12275-014-3257-3

67 View
0 Download
2 Crossref

Abstract

The inky cap, Coprinellus congregatus, produces mushrooms which become autolyzed rapidly to generate black liquid droplets, in which no cell wall is detected by microscopy. A chitinase (Chi2) which is synthesized during the autolytic phase of C. congregatus inhibits the growths of Candida al-bicans and Cryptococcus neoformans up to 10% at the con-centration of 10 μg/ml, about 50% at concentration of 20 μg/ml, and up to 95% at the concentration of 70 μg/ml. Upon treatment these yeast cells are observed to be severely de-formed, with the formation of large holes in the cell wall. The two yeast species show no growth inhibition at the concen-tration of 5 μg/ml, which means the minimum inhibitory concentrations for both yeast species are 10 μg/ml under these experimental conditions.

Citations

Citations to this article as recorded by

Analysis of the Antifungal Potential of Macrocybe Titans Extract Against Candida Albicans
Fernanda CBN Pereira, Gabrielle C Peiter, Vivian EMS Justo, Gabrieli M Huff, Pollyanna CV Conrado, Mauro AP da Silva, Patrícia S Bonfim-Mendonça, Terezinha IE Svidzinski, Fabio R Rosado, Adriana Fiorini
Future Microbiology.2023; 18(6): 357. CrossRef
Disarming Fungal Pathogens: Bacillus safensis Inhibits Virulence Factor Production and Biofilm Formation by Cryptococcus neoformans and Candida albicans
François L. Mayer, James W. Kronstad, Yong-Sun Bahn, J. Andrew Alspaugh, Deborah Hogan
mBio.2017;[Epub] CrossRef

Biochemical Characterization of Chitinase 2 Expressed during the Autolytic Phase of the Inky Cap, Coprinellus congregatus: Yuri Kang , Hyewon Kim , Hyoung T. Choi; J. Microbiol. 2013;51(2):189-193. Published online April 27, 2013; DOI: https://doi.org/10.1007/s12275-013-2535-9

52 View
0 Download
18 Scopus

Abstract: Fungal cell walls consist of various glucans and chitin. The inky cap, Coprinellus congregatus, produces mushrooms at 25°C in a regime of 15 h light/9 h dark, and then the mushroom is autolyzed rapidly to generate black liquid droplets in which no cell walls are detected by microscopy. Chitinase cDNA from the mature mushroom tissues of C. congregatus, which consisted of 1,622 nucleotides (chi2), was successfully cloned using the rapid amplification of cDNA ends polymerase chain reaction technique. The deduced 498 amino acid sequence of Chi2 had a conserved catalytic domain as in other fungal chitinase family 18 enzymes. The Chi2 enzyme was purified from the Pichia pastoris expression system, and its estimated molecular weight was 68 kDa. The optimum pH and temperature of Chi2 was pH 4.0 and 35°C, respectively when 4-nitrophenyl N,N’-diacetyl-β-D-chitobioside was used as the substrate. The Km value and Vmax for the substrate A, 4-nitrophenyl N,N’-diacetyl-β-D-chitobioside, was 0.175 mM and 0.16 OD min-1unit-1, respectively.

Research Support, Non-U.S. Gov'ts

Construction of a Streptomyces lydicus A01 Transformant with a chit42 Gene from Trichoderma harzianum P1 and Evaluation of Its Biocontrol Activity against Botrytis cinerea: Qiong Wu , Linquan Bai , Weicheng Liu , Yingying Li , Caige Lu , Yaqian Li , Kehe Fu , Chuanjin Yu , Jie Chen; J. Microbiol. 2013;51(2):166-173. Published online April 27, 2013; DOI: https://doi.org/10.1007/s12275-013-2321-8

51 View
0 Download
16 Scopus

Abstract: Streptomyces lydicus A01 and Trichoderma harzianum P1 are potential biocontrol agents of fungal diseases in plants. S. lydicus A01 produces natamycin to bind the ergosterol of the fungal cell membrane and inhibits the growth of Botrytis cinerea. T. harzianum P1, on the other hand, features high chitinase activity and decomposes the chitin in the cell wall of B. cinerea. To obtain the synergistic biocontrol effects of chitinase and natamycin on Botrytis cinerea, this study transformed the chit42 gene from T. harzianum P1 to S. lydicus A01. The conjugal transformant (CT) of S. lydicus A01 with the chit42 gene was detected using polymerase chain reaction (PCR). Associated chitinase activity and natamycin production were examined using the 3, 5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry, respectively. The S. lydicus A01-chit42 CT showed substantially higher chitinase activity and natamycin production than its wild type strain (WT). Consequently, the biocontrol effects of S. lydicus A01-chit42 CT on B. cinerea, including inhibition to spore germination and mycelial growth, were highly improved compared with those of the WT. Our research indicates that the biocontrol effect of Streptomyces can be highly improved by transforming the exogenous resistance gene, i.e. chit42 from Trichoderma, which not only enhances the production of antibiotics, but also provides a supplementary function by degrading the cell walls of the pathogens.

Selection of a Streptomyces Strain Able to Produce Cell Wall Degrading Enzymes and Active against Sclerotinia sclerotiorum: Adriana Fróes , Andrew Macrae , Juliana Rosa , Marcella Franco , Rodrigo Souza , Rosângela Soares , Rosalie Coelho; J. Microbiol. 2012;50(5):798-806. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2060-2

49 View
0 Download
19 Scopus

Abstract: Control of plant pathogen Sclerotinia sclerotiorum is an ongoing challenge because of its wide host range and the persistence of its sclerotia in soil. Fungicides are the most commonly used method to control this fungus but these can have ecotoxicity impacts. Chitinolytic Streptomyces strains isolated from Brazilian tropical soils were capable of inhibiting S. sclerotiorum growth in vitro, offering new possibilities for integrated pest management and biocontrol, with a new approach to dealing with an old problem. Strain Streptomyces sp. 80 was capable of irreversibly inhibiting fungal growth. Compared to other strains, its crude enzymes had the highest chitinolytic levels when measured at 25°C and strongly inhibited sclerotia from S. sclerotiorum. It produced four hydrolytic enzymes involved in fungal cell wall degradation when cultured in presence of the fungal mycelium. The best production, obtained after three days, was 0.75 U/ml for exochitinase, 0.9 U/ml for endochitinase, 0.16 U/ml for glucanase, and 1.78 U/ml for peptidase. Zymogram analysis confirmed two hydrolytic bands of chitinolytic activity with apparent molecular masses of 45.8 and 206.8 kDa. One glucanase activity with an apparent molecular mass of 55 kDa was also recorded, as well as seven bands of peptidase activity with apparent molecular masses ranging from 15.5 to 108.4 kDa. Differential interference contrast microscopy also showed alterations of hyphal morphology after co-culture. Streptomyces sp. 80 seems to be promising as a biocontrol agent against S. sclerotiorum, contributing to the development of new methods for controlling plant diseases and reducing the negative impact of using fungicides.

NOTE] Antifungal Activity of Extracellular Hydrolases Produced by Autolysing Aspergillus nidulans Cultures: Melinda Szilágyi , Fruzsina Anton , Katalin Forgács , Jae-Hyuk Yu , István Pócsi , Tamás Emri; J. Microbiol. 2012;50(5):849-854. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2001-0

42 View
0 Download
8 Crossref

Abstract

Carbon-starving Aspergillus nidulans cultures produce high activities of versatile hydrolytic enzymes and, among these, ChiB endochitinase and EngA β-1,3-endoglucanase showed significant antifungal activity against various fungal species. Double deletion of engA and chiB diminished the antifungal activity of the fermentation broths and increased conidiogenesis and long-term viability of A. nidulans, but decreased the growth rate on culture media containing weak carbon sources. Production of ChiB and EngA can influence fungal communities either directly due to their antifungal properties or indirectly through their effects on vegetative growth. Our data suggest saprophytic fungi as promising future candidates to develop novel biocontrol technologies.

Citations

Citations to this article as recorded by

Application and antagonistic mechanisms of atoxigenic Aspergillus strains for the management of fungal plant diseases
Suyan Wang, Yanxia Wang, Xinchi Shi, Daniela D. Herrera-Balandrano, Xin Chen, Fengquan Liu, Pedro Laborda, Irina S. Druzhinina
Applied and Environmental Microbiology.2024;[Epub] CrossRef
Identification and evaluation of Aspergillus tubingensis as a potential biocontrol agent against grey mould on tomato
Juan Zhao, Weicheng Liu, Dewen Liu, Caige Lu, Dianpeng Zhang, Huiling Wu, Dan Dong, Lingling Meng
Journal of General Plant Pathology.2018; 84(2): 148. CrossRef
Autolytic enzymes are responsible for increased melanization of carbon stressed Aspergillus nidulans cultures
Melinda Szilágyi, Fruzsina Anton, István Pócsi, Tamás Emri
Journal of Basic Microbiology.2018; 58(5): 440. CrossRef
Tricking Arthrinium malaysianum into Producing Industrially Important Enzymes Under 2-Deoxy D-Glucose Treatment
Soumya Mukherjee, Mathu Malar Chandrababunaidu, Arijit Panda, Suman Khowala, Sucheta Tripathy
Frontiers in Microbiology.2016;[Epub] CrossRef
γ-Glutamyl transpeptidase (GgtA) of Aspergillus nidulans is not necessary for bulk degradation of glutathione
Zsolt Spitzmüller, Nak-Jung Kwon, Melinda Szilágyi, Judit Keserű, Viktória Tóth, Jae-Hyuk Yu, István Pócsi, Tamás Emri
Archives of Microbiology.2015; 197(2): 285. CrossRef
Investigating Aspergillus nidulans secretome during colonisation of cork cell walls
Isabel Martins, Helga Garcia, Adélia Varela, Oscar Núñez, Sébastien Planchon, Maria Teresa Galceran, Jenny Renaut, Luís P.N. Rebelo, Cristina Silva Pereira
Journal of Proteomics.2014; 98: 175. CrossRef
Transcriptome changes initiated by carbon starvation in Aspergillus nidulans
Melinda Szilágyi, Márton Miskei, Zsolt Karányi, Béla Lenkey, István Pócsi, Tamás Emri
Microbiology.2013; 159(Pt_1): 176. CrossRef
Interactions between naturally occurring antifungal agents
Viktória Tóth, Melinda Szilágyi, Fruzsina Anton, Éva Leiter, I. Pócsi, T. Emri
Acta Biologica Hungarica.2013; 64(4): 510. CrossRef

Molecular Screening of Streptomyces Isolates for Antifungal Activity and Family 19 Chitinase Enzymes: Youssuf Gherbawy , Hesham Elhariry , Abdulla Altalhi , Bahig El-Deeb , Ghada Khiralla; J. Microbiol. 2012;50(3):459-468. Published online June 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2095-4

55 View
0 Download
33 Scopus

Abstract: Thirty soil-isolates of Streptomyces were analyzed to determine their antagonism against plant-pathogenic fungi including Fusarium oxysporum, Pythium aristosporum, Colletotrichum gossypii, and Rhizoctonia solani. Seven isolates showed antifungal activity against one or more strain of the tested fungi. Based on the 16S rDNA sequence analysis, these isolates were identified as Streptomyces tendae (YH3), S. griseus (YH8), S. variabilis (YH21), S. endus (YH24), S. violaceusniger (YH27A), S. endus (YH27B), and S. griseus (YH27C). The identity percentages ranged from 98 to 100%. Although some isolates belonged to the same species, there were many differences in their cultural and morphological characteristics. Six isolates out of seven showed chitinase activity according to a chitinolytic activity test and on colloidal chitin agar plates. Based on the conserved regions among the family 19 chitinase genes of Streptomyces sp. two primers were used for detection of the chitinase (chiC) gene in the six isolates. A DNA fragment of 1.4 kb was observed only for the isolates YH8, YH27A, and YH27C. In conclusion, six Streptomyces strains with potential chitinolytic activity were identified from the local environment in Taif City, Saudi Arabia. Of these isolates, three belong to family 19 chitinases. To our knowledge, this is the first reported presence of a chiC gene in S. violaceusniger YH27A.

Journal Article

Chitinase Production by Bacillus thuringiensis and Bacillus licheniformis: Their Potential in Antifungal Biocontrol: Eman Zakaria Gomaa; J. Microbiol. 2012;50(1):103-111. Published online February 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1343-y

56 View
0 Download
108 Crossref

Abstract

Thirty bacterial strains were isolated from the rhizosphere of plants collected from Egypt and screened for production of chitinase enzymes. Bacillus thuringiensis NM101-19 and Bacillus licheniformis NM120-17 had the highest chitinolytic activities amongst those investigated. The production of chitinase by B. thuringiensis and B. licheniformis was optimized using colloidal chitin medium amended with 1.5% colloidal chitin, with casein as a nitrogen source, at 30°C after five days of incubation. An enhancement of chitinase production by the two species was observed by addition of sugar substances and dried fungal mats to the colloidal chitin media. The optimal conditions for chitinase activity by B. thuringiensis and B. licheniformis were at 40°C, pH 7.0 and pH 8.0, respectively. Na+, Mg2+, Cu2+, and Ca2+ caused enhancement of enzyme activities whereas they were markedly inhibited by Zn2+, Hg2+, and Ag+. In vitro, B. thuringiensis and B. licheniformis chitinases had potential for cell wall lysis of many phytopathogenic fungi tested. The addition of B. thuringiensis chitinase was more effective than that of B. licheniformis in increasing the germination of soybean seeds infected with various phytopathogenic fungi.

Citations

Citations to this article as recorded by

Antagonism of Bacillus velezensis ZGE166 Against the Pathogenic Fungi Causing Corm Rot Disease in Saffron (Crocus sativus L.)
Yingqiu Guo, Li Tian, Xinyu Zhu, Shu Liu, Lili Wang, Wankui Li
Microbial Ecology.2025;[Epub] CrossRef
Chitinase-binding gene BvCHiB enhances resistance of Arabidopsis to verticillium wilt through JA and SA pathways
Yujing Liu
Highlights in Science, Engineering and Technology.2025; 129: 213. CrossRef
Effect of Pseudomonas protegens EMM-1 Against Rhizopus oryzae in Interactions with Mexican Autochthonous Red Maize
Bruce Manuel Morales-Barron, Violeta Larios-Serrato, Yolanda Elizabeth Morales-García, Verónica Quintero-Hernández, Paulina Estrada-de los Santos, Jesús Muñoz-Rojas
Life.2025; 15(4): 554. CrossRef
Biocontrol Potential of Streptomyces sp. C2-4 against Ceratobasidium cereale Caused Sharp Eyespot in Wheat
Miju Jo, Sunmin An, Da Som Kim, Won-Jae Chi, Sook-Young Park
Research in Plant Disease.2025; 31(1): 125. CrossRef
Process optimisation for improved chitinase production from marine isolate Bacillus haynesii and bioethanol production with Saccharomyces cerevisiae
Vishnupriya Govindaraj, Dinesh Kumar Anandan, Se-Kwon Kim, Ritu Raval, Keyur Raval
Brazilian Journal of Microbiology.2025; 56(2): 835. CrossRef
The Potential of Beneficial Microbes for Sustainable Alternative Approaches to Control Phytopathogenic Diseases
Ramadan Bakr, Ali Abdelmoteleb, Vianey Mendez-Trujillo, Daniel Gonzalez-Mendoza, Omar Hewedy
Microbiology Research.2025; 16(5): 105. CrossRef
Evaluation of Bacillus isolates as a biological control agents against soilborne phytopathogenic fungi
Derya Maral-Gül, Rengin Eltem
International Microbiology.2024; 28(S1): 75. CrossRef
Chitinase: a potent biocatalyst and its diverse applications
Gaytri Mahajan, Vibhuti Sharma, Reena Gupta
Biocatalysis and Biotransformation.2024; 42(2): 85. CrossRef
Bacillus cereus sensu lato antimicrobial arsenal: An overview
Louis Morandini, Simon Caulier, Claude Bragard, Jacques Mahillon
Microbiological Research.2024; 283: 127697. CrossRef
Effective biological control of Lentil (Lens culinaris) Fusarium wilt and plant growth promotion through native Rhizobacteria
Javeria Ayub, Ayesha Tahir, Umer Iqbal, Huma Ayub, Muhammad Zeeshan Hyder, Amna Kiyani, Fauzia Yusuf Hafeez, Muhammad Kashif Ilyas, Abdul Ghafoor, Tayyaba Yasmin
Physiological and Molecular Plant Pathology.2024; 129: 102203. CrossRef
Bacillus endophytic strains control Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici in tomato cv. Perinha
Guilherme Caldieraro Viana, Leonardo Oliveira Médici, Marcia Soares Vidal, José Ivo Baldani
Brazilian Journal of Microbiology.2024; 55(4): 4019. CrossRef
Exploring chitin: novel pathways and structures as promising targets for biopesticides
Malkiet Kaur, Manju Nagpal, Gitika Arora Dhingra, Ankit Rathee
Zeitschrift für Naturforschung C.2024; 79(5-6): 125. CrossRef
Chitinolytic microorganisms for biological control of plant pathogens: A Comprehensive review and meta-analysis
Sristi Das, Suvasri Dutta, Saibal Ghosh, Abhishek Mukherjee
Crop Protection.2024; 185: 106888. CrossRef
Improved Expression of a Thermostable GH18 Bacterial Chitinase in Two Different Escherichia coli Strains and Its Potential Use in Plant Protection and Biocontrol of Phytopathogenic Fungi
Aymen Ezzine, Safa Ben Hadj Mohamed, Sofiane Bezzine, Yosra Aoudi, Mohamed Rabeh Hajlaoui, Laura Baciou, Issam Smaali
Molecular Biotechnology.2024; 66(9): 2635. CrossRef
A highly active Chitinase-A of Serratia ficaria isolated from Pieris brassicae (Lepidoptera: Pieridae)
Merve Almula Bakirdogen, Gozde Busra Eroglu
Crop Protection.2024; 179: 106623. CrossRef
Bacillus genus industrial applications and innovation: First steps towards a circular bioeconomy
Leonardo Wedderhoff Herrmann, Luiz Alberto Junior Letti, Rafaela de Oliveira Penha, Vanete Thomaz Soccol, Cristine Rodrigues, Carlos Ricardo Soccol
Biotechnology Advances.2024; 70: 108300. CrossRef
In Vitro Antagonistic Activity of Plant Growth Promoting Rhizobacteria Against Aggressive Biotypes of the Green Mold
Baran Mis, Kemal Karaca, Rengin Eltem
Journal of Basic Microbiology.2024;[Epub] CrossRef
Flue-cured tobacco intercropping with insectary floral plants improves rhizosphere soil microbial communities and chemical properties of flue-cured tobacco
Jin Zhong, Wenze Pan, Shenglan Jiang, Yanxia Hu, Guangyuan Yang, Ke Zhang, Zhenyuan Xia, Bin Chen
BMC Microbiology.2024;[Epub] CrossRef
Insights into the whole genome sequence of Bacillus thuringiensis NBAIR BtPl, a strain toxic to the melon fruit fly, Zeugodacus cucurbitae
Nekkanti Aarthi, Vinod K. Dubey, Arakalagud N. Shylesha, Aditya Kukreti, Jagadeesh Patil, Keerthi M. Chandrashekara, Kandan Aravindaram, Ruqiya Seegenahalli, Nanditha Shivakumar, Manjunatha Channappa
Current Genetics.2024;[Epub] CrossRef
Marine chitinase AfChi: green defense management against Colletotrichum gloeosporioides and anthracnose
Rajesh K.M., Keyur Raval, Ritu Raval
AMB Express.2024;[Epub] CrossRef
Biocontrol of fungal phytopathogens by Bacillus pumilus
Jakub Dobrzyński, Zuzanna Jakubowska, Iryna Kulkova, Paweł Kowalczyk, Karol Kramkowski
Frontiers in Microbiology.2023;[Epub] CrossRef
PENGARUH pH, SUHU DAN JENIS SUBSTRAT TERHADAP AKTIVITAS KITINASE Bacillus sp. RNT9
Satrio Adil Pamungkas, Indun Dewi Puspita, Ustadi Ustadi
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology.2023; 19(1): 29. CrossRef
The Prospect of Hydrolytic Enzymes from Bacillus Species in the Biological Control of Pests and Diseases in Forest and Fruit Tree Production
Henry B. Ajuna, Hyo-In Lim, Jae-Hyun Moon, Sang-Jae Won, Vantha Choub, Su-In Choi, Ju-Yeol Yun, Young Sang Ahn
International Journal of Molecular Sciences.2023; 24(23): 16889. CrossRef
Characterization of chitinolytic bacteria newly isolated from the termite Microcerotermes sp. and their biocontrol potential against plant pathogenic fungi
Kittipong Chanworawit, Pachara Wangsoonthorn, Pinsurang Deevong
Bioscience, Biotechnology, and Biochemistry.2023; 87(9): 1077. CrossRef
Enhanced production of N-acetyl-glucosaminidase by marine Aeromonas caviae CHZ306 in bioreactor
Flávio Augusto Cardozo, Valker Feitosa, Carlos Miguel Nóbrega Mendonça, Francisco Vitor Santos da Silva, Attilio Converti, Ricardo Pinheiro de Souza Oliveira, Adalberto Pessoa
Brazilian Journal of Microbiology.2023; 54(3): 1533. CrossRef
Gas Chromatography–Mass Spectrometry Profiling of Volatile Metabolites Produced by Some Bacillus spp. and Evaluation of Their Antibacterial and Antibiotic Activities
Moldir Koilybayeva, Zhanserik Shynykul, Gulbaram Ustenova, Krzysztof Waleron, Joanna Jońca, Kamilya Mustafina, Akerke Amirkhanova, Yekaterina Koloskova, Raushan Bayaliyeva, Tamila Akhayeva, Mereke Alimzhanova, Aknur Turgumbayeva, Gulden Kurmangaliyeva, Ai
Molecules.2023; 28(22): 7556. CrossRef
Thermostable Chitinase Producing Bacterium from Ijen Hot Spring – Indonesia: Isolation, Identification, and Characterization
Ruth Chrisnasari, Liony Priscilla Sutanto, Dian Paulina, Alicia Wahjudi, Tjandra Pantjajani, R.H. Setyobudi, A. Yaro, I. Zekker, M. Zahoor, T. Turkadze
E3S Web of Conferences.2023; 374: 00032. CrossRef
Isolation, biochemical characterization, and genome sequencing of two high‐quality genomes of a novel chitinolytic Jeongeupia species
Nathanael D. Arnold, Daniel Garbe, Thomas B. Brück
MicrobiologyOpen.2023;[Epub] CrossRef
Approach for quick exploration of highly effective broad-spectrum biocontrol strains based on PO8 protein inhibition
Mei Gu, Jiayun Fu, Honglin Yan, Xiaofeng Yue, Shancang Zhao, Qi Zhang, Peiwu Li
npj Science of Food.2023;[Epub] CrossRef
Antifungal Activity of Partially Purified Bacterial Chitinase Against Alternaria alternata
Neslihan Dikbaş, Sevda Uçar, Elif Tozlu, Merve Şenol Kotan, Recep Kotan
Erwerbs-Obstbau.2023; 65(4): 761. CrossRef
Characterization of the chemical fungicides-responsive and bacterial pathogen-preventing Bacillus licheniformis in rice spikelet
Chengfang Zhan, Mengchen Wu, Hongda Fang, Xiaoyu Liu, Jiuyue Pan, Xiaoyan Fan, Mengcen Wang, Haruna Matsumoto
Food Quality and Safety.2023;[Epub] CrossRef
Macrolactin A mediated biocontrol of Fusarium oxysporum and Rhizoctonia solani infestation on Amaranthus hypochondriacus by Bacillus subtilis BS-58
Chitra Pandey, Deepti Prabha, Yogesh Kumar Negi, Dinesh Kumar Maheshwari, Shrivardhan Dheeman, Monika Gupta
Frontiers in Microbiology.2023;[Epub] CrossRef
Formation of recombinant bifunctional fusion protein: A newer approach to combine the activities of two enzymes in a single protein
Patel Nilpa, Kapadia Chintan, R. Z. Sayyed, Hesham El Enshasy, Hala El Adawi, Alaa Alhazmi, Atiah H. Almalki, Shafiul Haque, Rahul Datta
PLOS ONE.2022; 17(4): e0265969. CrossRef
Efficiency and mechanisms of action of pelletized compost loaded with Bacillus velezensis CE 100 for controlling tomato Fusarium wilt
Seo Hyun Hwang, Chaw Ei Htwe Maung, Jun Su Noh, Woon Seon Baek, Jeong-Yong Cho, Kil Yong Kim
Biological Control.2022; 176: 105088. CrossRef
Purification, characterization, and antifungal activity of Bacillus cereus strain NK91 chitinase from rhizospheric soil samples of Himachal Pradesh, India
Nirja Thakur, Amarjit K Nath, Anjali Chauhan, Rakesh Gupta
Biotechnology and Applied Biochemistry.2022; 69(5): 1830. CrossRef
Macrolactin a Mediated Biocontrol of Two Important Fungal Pathogens of Amaranthus Hypochondriacus by Bacillus Subtilis BS-58
Chitra Pandey, Deepti Prabha, Yogesh Kumar Negi, D. K. Maheshwari
SSRN Electronic Journal.2022;[Epub] CrossRef
Morphological and structural characterization of chitin as a substrate for the screening, production, and molecular characterization of chitinase by Bacillus velezensis
Digvijay Dahiya, Akhil Pilli, Pratap Raja Reddy Chirra, Vinay Sreeramula, Nitish Venkateswarlu Mogili, Seenivasan Ayothiraman
Environmental Science and Pollution Research.2022; 29(57): 86550. CrossRef
Recent Advances and Technologies in Chitinase Production Under Solid-State Fermentation
Mini K. Paul, Umesh B.T, Jyothis Mathew
Biosciences Biotechnology Research Asia.2022; 19(4): 815. CrossRef
Effect of soil management systems on the rhizosphere bacterial community structure of tobacco: Continuous cropping vs. paddy-upland rotation
Peng Wang, Shen Yan, Wenshui Zhang, Xiaodan Xie, Mingjie Li, Tianbao Ren, Li Gu, Zhongyi Zhang
Frontiers in Plant Science.2022;[Epub] CrossRef
Broad-spectrum resistance mechanism of serine protease Sp1 in Bacillus licheniformis W10 via dual comparative transcriptome analysis
Lina Yang, Chun Yan, Shuai Peng, Lili Chen, Junjie Guo, Yihe Lu, Lianwei Li, Zhaolin Ji
Frontiers in Microbiology.2022;[Epub] CrossRef
Isolation of Chitinolytic Bacteria from European Sea Bass Gut Microbiota Fed Diets with Distinct Insect Meals
Fábio Rangel, Rafaela A. Santos, Marta Monteiro, Ana Sofia Lavrador, Laura Gasco, Francesco Gai, Aires Oliva-Teles, Paula Enes, Cláudia R. Serra
Biology.2022; 11(7): 964. CrossRef
Current Perspectives on Chitinolytic Enzymes and Their Agro-Industrial Applications
Vikram Poria, Anuj Rana, Arti Kumari, Jasneet Grewal, Kumar Pranaw, Surender Singh
Biology.2021; 10(12): 1319. CrossRef
Extracellular Antifungal Activity of Chitinase-Producing Bacteria Isolated From Guano of Insectivorous Bats
Delfini CD, Villegas LB, Martínez MA, Baigorí MD
Current Microbiology.2021; 78(7): 2787. CrossRef
Bacilli as sources of agrobiotechnology: recent advances and future directions
Zerihun T. Dame, Mahfuz Rahman, Tofazzal Islam
Green Chemistry Letters and Reviews.2021; 14(2): 246. CrossRef
A Novel Antidipteran Bacillus thuringiensis Strain: Unusual Cry Toxin Genes in a Highly Dynamic Plasmid Environment
Nancy Fayad, Zakaria Kambris, Laure El Chamy, Jacques Mahillon, Mireille Kallassy Awad, Karyn N. Johnson
Applied and Environmental Microbiology.2021;[Epub] CrossRef
Quantitative single molecule RNA-FISH and RNase-free cell wall digestion in Neurospora crassa
Bradley M. Bartholomai, Amy S. Gladfelter, Jennifer J. Loros, Jay C. Dunlap
Fungal Genetics and Biology.2021; 156: 103615. CrossRef
Antifungal Activity of Bacillus velezensis CE 100 against Anthracnose Disease (Colletotrichum gloeosporioides) and Growth Promotion of Walnut (Juglans regia L.) Trees
Vantha Choub, Henry B. Ajuna, Sang-Jae Won, Jae-Hyun Moon, Su-In Choi, Chaw Ei Htwe Maung, Chul-Woo Kim, Young Sang Ahn
International Journal of Molecular Sciences.2021; 22(19): 10438. CrossRef
Key parameters optimization of chitosan production from Aspergillus terreus using apple waste extract as sole carbon source
Alireza Habibi, Salar Karami, Kambiz Varmira, Malihe Hadadi
Bioprocess and Biosystems Engineering.2021; 44(2): 283. CrossRef
Chitinases production: A robust enzyme and its industrial applications
Rahul Vikram Singh, Krishika Sambyal, Anjali Negi, Shubham Sonwani, Ritika Mahajan
Biocatalysis and Biotransformation.2021; 39(3): 161. CrossRef
Microbial chitinases: properties, enhancement and potential applications
Eman Zakaria Gomaa
Protoplasma.2021; 258(4): 695. CrossRef
Drought Exposed Burkholderia seminalis JRBHU6 Exhibits Antimicrobial Potential Through Pyrazine-1,4-Dione Derivatives Targeting Multiple Bacterial and Fungal Proteins
Jay Kishor Prasad, Priyanka Pandey, Richa Anand, Richa Raghuwanshi
Frontiers in Microbiology.2021;[Epub] CrossRef
Pectin lyase enhances cotton resistance to Verticillium wilt by inducing cell apoptosis of Verticillium dahliae
Jing Zhang, Xinru Yu, Chaojun Zhang, Qiong Zhang, Ying Sun, Heqin Zhu, Canming Tang
Journal of Hazardous Materials.2021; 404: 124029. CrossRef
Bacillus licheniformis PR2 Controls Fungal Diseases and Increases Production of Jujube Fruit under Field Conditions
Jun-Hyeok Kwon, Sang-Jae Won, Jae-Hyun Moon, Uk Lee, Yun-Serk Park, Chaw Ei Htwe Maung, Henry B. Ajuna, Young Sang Ahn
Horticulturae.2021; 7(3): 49. CrossRef
Bacillus subtilis and B. licheniformis Isolated from Heterorhabditis indica Infected Apple Root Borer (Dorysthenes huegelii) Suppresses Nematode Production in Galleria mellonella
Akanksha Upadhyay, Sharad Mohan
Acta Parasitologica.2021; 66(3): 989. CrossRef
Dissecting the Environmental Consequences of Bacillus thuringiensis Application for Natural Ecosystems
Maria E. Belousova, Yury V. Malovichko, Anton E. Shikov, Anton A. Nizhnikov, Kirill S. Antonets
Toxins.2021; 13(5): 355. CrossRef
Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers
Tahira Syed, Muhammad Askari, Zhigang Meng, Yanyan Li, Muhammad Abid, Yunxiao Wei, Sandui Guo, Chengzhen Liang, Rui Zhang
Toxins.2020; 12(8): 522. CrossRef
Comparative bioefficacy of Bacillus and Pseudomonas chitinase against Helopeltis theivora in tea (Camellia sinensis (L.) O.Kuntze
M. Suganthi, S. Arvinth, P. Senthilkumar
Physiology and Molecular Biology of Plants.2020; 26(10): 2053. CrossRef
Curing piglets from diarrhea and preparation of a healthy microbiome with Bacillus treatment for industrial animal breeding
Shousong Yue, Zhentian Li, Fuli Hu, Jean-François Picimbon
Scientific Reports.2020;[Epub] CrossRef
Bacillus Thuringiensis – Nowy PotencjaŁ Aplikacyjny
Aleksandra Gęsicka, Agata Henschke, Zuzanna Barańska, Agnieszka Wolna-Maruwka
Postępy Mikrobiologii - Advancements of Microbiology.2020; 59(4): 357. CrossRef
Bioconversion of Colloidal Chitin Using Novel Chitinase from Glutamicibacter uratoxydans Exhibiting Anti-fungal Potential by Hydrolyzing Chitin Within Fungal Cell Wall
Tayyaba Asif, Urooj Javed, Syeda Bushra Zafar, Asma Ansari, Shah Ali Ul Qader, Afsheen Aman
Waste and Biomass Valorization.2020; 11(8): 4129. CrossRef
Biocontrol potential of chitinases produced by newly isolated Chitinophaga sp. S167
Sonia Sharma, Shiv Kumar, Anjali Khajuria, Puja Ohri, Rajinder Kaur, Ramandeep Kaur
World Journal of Microbiology and Biotechnology.2020;[Epub] CrossRef
Screening of an Alkaline CMCase-Producing Strain and the Optimization of its Fermentation Condition
Junmei Zhou, Lianghong Yin, Chenbin Wu, Sijia Wu, Jidong Lu, Hailing Fang, Yongchang Qian
Current Pharmaceutical Biotechnology.2020; 21(13): 1304. CrossRef
Screening of Bacillus thuringiensis strains to identify new potential biocontrol agents against Sclerotinia sclerotiorum and Plutella xylostella in Brassica campestris L.
Meiling Wang, Lili Geng, Xiaoxiao Sun, Changlong Shu, Fuping Song, Jie Zhang
Biological Control.2020; 145: 104262. CrossRef
Chitinases of Bacillus thuringiensis: Phylogeny, Modular Structure, and Applied Potentials
Sheila A. Martínez-Zavala, Uriel E. Barboza-Pérez, Gustavo Hernández-Guzmán, Dennis K. Bideshi, José E. Barboza-Corona
Frontiers in Microbiology.2020;[Epub] CrossRef
Isolation and Identification of Plant Growth-Promoting Bacteria Highly Effective in Suppressing Root Rot in Fava Beans
Mona M. G. Saad, Mahrous Kandil, Youssef M. M. Mohammed
Current Microbiology.2020; 77(9): 2155. CrossRef
Accelerating the Morphogenetic Cycle of the Viral Vector Aedes aegypti Larvae for Faster Larvicidal Bioassays
José Domingos Fontana, Rafael Lopes Ferreira, Tatiana Zuccolotto, Cibelle de Borba Dallagassa, Leonardo Pellizzari Wielewski, Barbara Maria Santano Chalcoski, Mario Antonio Navarro da Silva, Vinicius Sobrinho Richardi, Jonas Golart, Cynara de Melo Rodoval
BioMed Research International.2020;[Epub] CrossRef
Isolation of a novel rhizobacteria having multiple plant growth promoting traits and antifungal activity against certain phytopathogens
Madhurankhi Goswami, Suresh Deka
Microbiological Research.2020; 240: 126516. CrossRef
Long-term continuously monocropped peanut significantly changed the abundance and composition of soil bacterial communities
Mingna Chen, Hu Liu, Shanlin Yu, Mian Wang, Lijuan Pan, Na Chen, Tong Wang, Xiaoyuan Chi, Binghai Du
PeerJ.2020; 8: e9024. CrossRef
Lipopeptide mediated biocontrol activity of endophytic Bacillus subtilis against fungal phytopathogens
Dibya Jyoti Hazarika, Gunajit Goswami, Trishnamoni Gautom, Assma Parveen, Pompi Das, Madhumita Barooah, Robin Chandra Boro
BMC Microbiology.2019;[Epub] CrossRef
Microbial metabolomics: essential definitions and the importance of cultivation conditions for utilizing Bacillus species as bionematicides
I. Horak, G. Engelbrecht, P.J. Jansen Rensburg, S. Claassens
Journal of Applied Microbiology.2019; 127(2): 326. CrossRef
Chitinases As The Key To The Interaction Between Plants And Microorganisms
Anna Kisiel, Katarzyna Jęckowska
Postępy Mikrobiologii - Advancements of Microbiology.2019; 58(3): 317. CrossRef
Biochemical characterization of chitinase A from Bacillus licheniformis DSM8785 expressed in Pichia pastoris KM71H
Gheorghita Menghiu, Vasile Ostafe, Radivoje Prodanovic, Rainer Fischer, Raluca Ostafe
Protein Expression and Purification.2019; 154: 25. CrossRef
Overview of the Antimicrobial Compounds Produced by Members of the Bacillus subtilis Group
Simon Caulier, Catherine Nannan, Annika Gillis, Florent Licciardi, Claude Bragard, Jacques Mahillon
Frontiers in Microbiology.2019;[Epub] CrossRef
Repertoire of the Bacillus thuringiensis Virulence Factors Unrelated to Major Classes of Protein Toxins and Its Role in Specificity of Host-Pathogen Interactions
Yury V. Malovichko, Anton A. Nizhnikov, Kirill S. Antonets
Toxins.2019; 11(6): 347. CrossRef
Unraveling the Optimal Culture Condition for the Antifungal Activity and IAA Production of Phylloplane Serratia plymuthica
Siti Nur Aisyah, Jefri Maldoni, Irma Sulastri, Weni Suryati, Yuli Marlisa, Lissa Herliana, Lily Syukriani, Renfiyeni Renfiyeni, Jamsari Jamsari
Plant Pathology Journal.2019; 18(1): 31. CrossRef
Inhibitory effect and possible mechanism of a Pseudomonas strain QBA5 against gray mold on tomato leaves and fruits caused by Botrytis cinerea
Pan Gao, Jiaxing Qin, Delong Li, Shanyue Zhou, Ya-Wen He
PLOS ONE.2018; 13(1): e0190932. CrossRef
Chitinases—Potential Candidates for Enhanced Plant Resistance towards Fungal Pathogens
Manish Kumar, Amandeep Brar, Monika Yadav, Aakash Chawade, V. Vivekanand, Nidhi Pareek
Agriculture.2018; 8(7): 88. CrossRef
Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host
Nida Toufiq, Bushra Tabassum, Muhammad Umar Bhatti, Anwar Khan, Muhammad Tariq, Naila Shahid, Idrees Ahmad Nasir, Tayyab Husnain
Brazilian Journal of Microbiology.2018; 49(2): 414. CrossRef
Isolation and Evaluation of New Antagonist Bacillus Strains for the Control of Pathogenic and Mycotoxigenic Fungi of Fig Orchards
Özlem Öztopuz, Gülseren Pekin, Ro Dong Park, Rengin Eltem
Applied Biochemistry and Biotechnology.2018; 186(3): 692. CrossRef
Microbial and viral chitinases: Attractive biopesticides for integrated pest management
Francesca Berini, Chen Katz, Nady Gruzdev, Morena Casartelli, Gianluca Tettamanti, Flavia Marinelli
Biotechnology Advances.2018; 36(3): 818. CrossRef
Improvement of Chitinase Production by Bacillus thuringiensis NM101-19 for Antifungal Biocontrol through Physical Mutation
E. Z. Gomaa, O. M. El-Mahdy
Microbiology.2018; 87(4): 472. CrossRef
Bacterial chitinases and their application in biotechnology
Anna Kisiel, Ewa Kępczyńska
Postępy Mikrobiologii - Advancements of Microbiology.2017; 56(3): 306. CrossRef
Combating Fusarium Infection Using Bacillus-Based Antimicrobials
Noor Khan, Maskit Maymon, Ann Hirsch
Microorganisms.2017; 5(4): 75. CrossRef
Screening and Characterisation of Chitinolytic Microorganisms with Potential to Control White Root Disease of Hevea brasiliensis
Nor Afiqah Maiden, Aizat Shamin Noran, Mohd Adi Faiz Ahmad Fauzi, Safiah Atan
Journal of Rubber Research.2017; 20(3): 182. CrossRef
Antifungal Screening of Bioprotective Isolates against Botrytis cinerea, Fusarium pallidoroseum and Fusarium moniliforme
Antoinette De Senna, Amanda Lathrop
Fermentation.2017; 3(4): 53. CrossRef
Simultaneous fermentative chitinase and β-1,3 glucanase production from Streptomyces philanthi RM-1-1-38 and their antifungal activity against rice sheath blight disease
Sawai Boukaew, Wanida Petlamul, Wasana Suyotha, Poonsuk Prasertsan
BioTechnologia.2017; 97(4): 271. CrossRef
Bacillus thuringiensis: a successful insecticide with new environmental features and tidings
Gholamreza Salehi Jouzani, Elena Valijanian, Reza Sharafi
Applied Microbiology and Biotechnology.2017; 101(7): 2691. CrossRef
Enhancement of Exochitinase Production by Bacillus licheniformis AT6 Strain and Improvement of N-Acetylglucosamine Production
Mohamed Amine Aounallah, Imen Ben Slimene-Debez, Kais Djebali, Dorra Gharbi, Majdi Hammami, Sana Azaiez, Ferid Limam, Olfa Tabbene
Applied Biochemistry and Biotechnology.2017; 181(2): 650. CrossRef
Chitinase from Pseudomonas fluorescens and its insecticidal activity against Helopeltis theivora
M. Suganthi, P. Senthilkumar, S. Arvinth, K. N. Chandrashekara
The Journal of General and Applied Microbiology.2017; 63(4): 222. CrossRef
Silver nanoparticles as an antimicrobial agent: A case study on Staphylococcus aureus and Escherichia coli as models for Gram-positive and Gram-negative bacteria
Eman Zakaria Gomaa
The Journal of General and Applied Microbiology.2017; 63(1): 36. CrossRef
Optimised production of chitinase from a novel mangrove isolate, Bacillus pumilus MCB-7 using response surface methodology
K.S. Rishad, Sharrel Rebello, Vinod Kumar Nathan, S. Shabanamol, M.S. Jisha
Biocatalysis and Agricultural Biotechnology.2016; 5: 143. CrossRef
A new chitinase-D from a plant growth promoting Serratia marcescens GPS5 for enzymatic conversion of chitin
Papa Rao Vaikuntapu, Samudrala Rambabu, Jogi Madhuprakash, Appa Rao Podile
Bioresource Technology.2016; 220: 200. CrossRef
Bacillus thuringiensis C25 which is rich in cell wall degrading enzymes efficiently controls lettuce drop caused by Sclerotinia minor
Anupama Shrestha, Razia Sultana, Jong-Chan Chae, Kangmin Kim, Kui-Jae Lee
European Journal of Plant Pathology.2015; 142(3): 577. CrossRef
Isolation of a Chitinolytic Bacillus licheniformis S213 Strain Exerting a Biological Control Against Phoma medicaginis Infection
Imen Ben Slimene, Olfa Tabbene, Dorra Gharbi, Bacem Mnasri, Jean Marie Schmitter, Maria-Camino Urdaci, Ferid Limam
Applied Biochemistry and Biotechnology.2015; 175(7): 3494. CrossRef
Characterization of regulatory regions involved in the inducible expression of chiB in Bacillus thuringiensis
Chi-Chu Xie, Jin Shi, Hai-Yun Jia, Peng-Fei Li, Yang Luo, Jun Cai, Yue-Hua Chen
Archives of Microbiology.2015; 197(1): 53. CrossRef
YvoA and CcpA Repress the Expression of chiB in Bacillus thuringiensis
Kun Jiang, Li-na Li, Jin-hua Pan, Ting-ting Wang, Yue-hua Chen, Jun Cai, S.-J. Liu
Applied and Environmental Microbiology.2015; 81(19): 6548. CrossRef
Ecology of Bacillaceae
Ines Mandic-Mulec, Polonca Stefanic, Jan Dirk van Elsas, Patrick Eichenberger, Adam Driks
Microbiology Spectrum.2015;[Epub] CrossRef
Chitinase biotechnology: Production, purification, and application
Yuriy Mihaylov Stoykov, Atanas Ivanov Pavlov, Albert Ivanov Krastanov
Engineering in Life Sciences.2015; 15(1): 30. CrossRef
Efficient biosynthesis of a chitinase from Halobacterium salinarum expressed in Escherichia coli
Fatima Moscoso, Myriam Sieira, Alberto Domínguez, Francisco J. Deive, Maria A. Longo, Maria A. Sanromán
Journal of Chemical Technology & Biotechnology.2014; 89(11): 1653. CrossRef
Potential use and mode of action of the new strainBacillus thuringiensisUM96 for the biological control of the grey mould phytopathogenBotrytis cinerea
Sofía Martínez-Absalón, Daniel Rojas-Solís, Rocío Hernández-León, Cristina Prieto-Barajas, Ma. del Carmen Orozco-Mosqueda, Juan José Peña-Cabriales, Shohei Sakuda, Eduardo Valencia-Cantero, Gustavo Santoyo
Biocontrol Science and Technology.2014; 24(12): 1349. CrossRef
Isolation and characterization of an antifungal protein from Bacillus licheniformis HS10
Zhixin Wang, Yunpeng Wang, Li Zheng, Xiaona Yang, Hongxia Liu, Jianhua Guo
Biochemical and Biophysical Research Communications.2014; 454(1): 48. CrossRef
Dual silencing of long and short Amblyomma americanum acidic chitinase forms weakens the tick cement cone stability
Tae K. Kim, Jenny Curran, Albert Mulenga
Journal of Experimental Biology.2014;[Epub] CrossRef
Partial Purification of Bacterial Chitinase as Biocontrol of Leaf Blight Disease on Oil Palm
Muhammad Asril, Nisa Rachmania Mubarik, Aris Tri Wahyudi
Research Journal of Microbiology.2014; 9(6): 265. CrossRef
Characterization and evaluation of Staphylococcus sp. strain LZ16 for the biological control of rice blast caused by Magnaporthe oryzae
Qin Yu, Zhu Liu, Derun Lin, Wei Zhang, Qun Sun, Jianqing Zhu, Min Lin
Biological Control.2013; 65(3): 338. CrossRef
Development of an Industrial Microbial System for Chitinolytic Enzymes Production
F. Moscoso, L. Ferreira, M.A. Fernández de Dios, F.J. Deive, M.A. Longo, M.A. Sanromán
Industrial & Engineering Chemistry Research.2013; 52(30): 10046. CrossRef
Bacillus thuringiensiscolonises plant roots in a phylogeny-dependent manner
J. Cristian Vidal-Quist, Hilary J. Rogers, Eshwar Mahenthiralingam, Colin Berry
FEMS Microbiology Ecology.2013; 86(3): 474. CrossRef
Comparative Genome Analysis of Enterobacter cloacae
Wing-Yee Liu, Chi-Fat Wong, Karl Ming-Kar Chung, Jing-Wei Jiang, Frederick Chi-Ching Leung, Jingfa Xiao
PLoS ONE.2013; 8(9): e74487. CrossRef
Antifungal activity of the lipopeptides produced by Bacillus amyloliquefaciens anti-CA against Candida albicans isolated from clinic
Bo Song, Yan-Jun Rong, Ming-Xin Zhao, Zhen-Ming Chi
Applied Microbiology and Biotechnology.2013; 97(16): 7141. CrossRef

Research Support, Non-U.S. Gov'ts

Growth Inhibition of the Yeast Transformant by the Expression of a Chitinase from Coprinellus congregatus: Hyangsoon Lim , Hyoung T. Choi; J. Microbiol. 2010;48(5):706-708. Published online November 3, 2010; DOI: https://doi.org/10.1007/s12275-010-0272-x

43 View
0 Download
6 Scopus

Abstract: Coprinellus congregatus generates several chitinases during its entire life cycle: at the growing hyphal stage and at the mushroom autolysis stage. We have isolated a chitinase gene (chi1) from the mushroom tissue at the autolysing stage, and constructed a chitinase expression vector to get large amount of enzyme protein. Chitinase 1 (chi1) cDNA was heterologously expressed in Saccharomyces cerevisiae by gal1 promoter. The transformants showed no specific change in growth characteristics under normal growth conditions. However the expression of the gene by the gal1 promoter in the yeast transformants resulted in complete growth inhibition, while laccase expression by the gal1 promoter showed normal growth. The chitinase activities from the transformants were also more than 3 times higher than that of the recipient strain, and the chitinase expression by the real time-PCR also showed increased expression of the chi1 in the yeast transformant. Expression of a chitinase which was produced at the mushroom autolysing stage of C. congregatus resulted in yeast growth inhibition.

Note] Enhanced Expression of Chitinase during the Autolysis of Mushroom in Coprinellus congregatus: Hyangsoon Lim , Hyoung T. Choi; J. Microbiol. 2009;47(2):225-228. Published online May 2, 2009; DOI: https://doi.org/10.1007/s12275-008-0247-3

45 View
0 Download
21 Scopus

Abstract: Fungal cell walls consist of various glucans and chitin. An inky cap, Coprinellus congregatus, produced mushrooms at 25°C in a regime of 15 h light/9 h dark, and then the mushroom was autolyzed rapidly to generate black liquid droplets where no cell wall was detected by microscopy. A chitinase cDNA from the matured mushroom cells of C. congregatus that consisted of 1,541 nucleotides was successfully cloned using the rapid amplification of cDNA ends (RACE)-PCR technique. Its deduced 441 amino acid sequence had the conserved catalytic domain as in other fungal chitinase family 18. Chitinase activity was higher at the matured mushroom stage than primordial and young mushroom stage. When the expression of the cloned chitinase was examined by real-time PCR using the chitinase-specific primers, it was increased more than twice to 20 times during the autolytic process of mushroom than young mushroom or primordial stages, respectively.

Cloning and Expression Analysis of a Chitinase Gene Crchi1 from the Mycoparasitic Fungus Clonostachys rosea (syn. Gliocladium roseum): Zhongwei Gan , Jinkui Yang , Nan Tao , Zefen Yu , Ke-Qin Zhang; J. Microbiol. 2007;45(5):422-430.; DOI: https://doi.org/2594 [pii]

50 View
0 Download

Abstract: Clonostachys rosea (syn. Gliocladium roseum) is a well-known biocontrol agent and widely distributed around the world. In this study, an endochitinase gene Crchi1 was isolated from the mycoparasitic fungus C. rosea using the DNA walking strategy. The Crchi1 ORF is 1,746 bp long and interrupted by three introns. The cloned gene Crchi1 encodes 426 amino acid residues and shares a high degree of similarity with other chitinases from entomopathogenic and mycoparasitic fungi. Several putative binding sites for transcriptional regulation of Crchi1 in response to carbon (5''-SYGGRG-3'') and nitrogen (5''-GATA-3'') were identified in the upstream of Crchi1. Expression of Crchi1 gene in different carbon sources was analyzed using real-time PCR (RT-PCR). We found that the Crchi1 expression was suppressed by glucose but strongly stimulated by chitin or solubilized components of the cell wall from Rhizoctonia solani. Phylogenetic analysis of chitinases from entomopathogenic and mycoparasitic fungi suggests that these chitinases have probably evolved from a common ancestor.

The role and characterization of β-1,3-glucanase in biocontrol of fusarium solani by pseudomonas stutzeri YPL-1: Lim, Ho Seong , Kim, Sang Dal; J. Microbiol. 1995;33(4):295-301.

52 View
0 Download

Abstract: An antifungal Pseudomonas stutzeri YPL-1 produced extracellular chitinase and β-1,3-glucanase that were key enzymes in the decomposition of fungal hyphal walls. These lytic extracellular enzymes markedly inhibited mycelial growth of the phytopathogenic fungus Fusarium solani. A chitinase from P. stutzeri YPL-1 inhibited fungal mycelial growth by 87%, whereas a β-1,3-glucanase from the bacterium inhibited growth by 53%. Furthermore, co-operative action of the enzymes synergistically inhibited 95% of the fungal growth. The lytic enzymes caused abnormal swelling and retreating on the fungal hyphal walls in a dual cultures. Scanning electron microscopy clearly showed hyphal degradation of F. solani in the regions interacting with P. stutzeri YPL-1. In an in vivo pot test, P. stutzeri YPL-1 proved to have biocontrol ability as a powerful agent in controlling plant disease. Planting of kidney bean (Phaseolus vulgaris L.) seedlings with the bacterial suspension in F. solani-infested soil significantly suppressed the development of fusarial root-rot. The characteristics of a crude preparation of β-1,3-glucanase produced from P. stutzeri YPL-1 were investigated. The bacterium detected after 2 hr of incubation. The enzyme had optimum temperature and pH of 40℃ and pH 5.5, respectively. The enzyme was stable in the pH range of 4.5 to 7.0 and at temperatures below 40℃, with a half-life of 40 min at 60℃.

Purification and Characterization of Chitinase from a Marine Bacterium, Vibrio sp. 98CJ11027: Shin Hye Park , Jung-Hyun Lee , Hong Kum Lee; J. Microbiol. 2000;38(4):224-229.

49 View
0 Download

Abstract: Chitin-degrading marine bacterial strain 98CJ11027 was isolated from bryozoa from the coastal area of Cheju Island, Korea, and identified as a member of the genus Vibrio. The molecular mass of the main extracellular chitinase (chitinase I), purified from strain 98CJ11027, was estimated to be 98 kDa. The optimal condition for chitinase I activity is pH 6.0 and 45 C. The activity was inhibited by Fe^+2 and Cu^+2. Chitinase I displayed the hydrolysis type of chitobiosidase and catalyzed reversed hydrolysis leading to the synthesis of tetraacetylchitotetraose.

First
Prev
Page of 1
Next
Last

Search